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研究生:周彥萍
論文名稱:利用稻稈水解液進行同時糖化與醱酵生產氫氣和乙醇之研究
論文名稱(外文):Rice Straw Hydrolyzateof Simultaneous Saccharification and Fermentation processes (SSF) for Production of Hydrogen with Ethanol
指導教授:吳石乙
口試委員:朱正永林祺能
口試日期:2015-06-26
學位類別:碩士
校院名稱:逢甲大學
系所名稱:化學工程學系
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2015
畢業學年度:103
語文別:英文
論文頁數:127
中文關鍵詞:稻稈稀酸水解醱酵氫氣乙醇
相關次數:
  • 被引用被引用:1
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  • 下載下載:35
  • 收藏至我的研究室書目清單書目收藏:1
稻稈在亞洲地區為主要農作物之一,傳統上其廢棄物處理方法以焚燒為主,此方法會產生溫室氣體如二氧化碳,而加劇溫室效應的產生。如能將其廢棄物以水解的方式處理,將其碳水化合物轉變成小分子醣類,進而醱酵生產為可利用能源如氫氣、乙醇,不但能解決環境汙染問題,更能轉換成能源加以利用。
在此研究中實驗分成三大部分,分別為水解前處理、纖維酶固定化以及將水解液醱酵產氫及乙醇。在水解前處理中,在稻稈濃度為100 g/L混合1 M 稀鹽酸並在121℃中反應20分鐘可得最佳總醣產率為52.9% (w/w)。接續稻稈前處理後置入固定化纖維酶中,而最佳的纖維酶固定化製程為將纖維酶添加20% (w/w)活性碳在8℃下吸附兩天後,利用2.0% (w/w)海藻酸鈉混和吸附纖維酶後的活性碳,在5% (w/w)氯化鈣中反應30分鐘,使其成為凝膠球。以批次試驗稻稈前處理後之水解液直接加入15%的菌液,在不同的水解液濃度下,進行同時糖化與醱酵生產氫氣與乙醇之試驗,在水解液濃度為10 g/g rice straw可得最大氫氣與乙醇產率分別為44.65 mL/g rice straw;55.24 mg/g rice straw,能源效益為124 kJ/g Polysaccharide。而置入固定化纖維酶試驗中,在10 g/g rice straw可得最大氫氣與乙醇產率分別為95.30 mL/g rice straw;70.82 mg/g rice straw,能源效益為158.01 kJ/g Polysaccharide,比未添加固定化纖維酶之批次試驗提高了1.2倍的能源效益;在最佳初始pH值中,在pH為6.5時可得最大氫氣與乙醇產率分別為72.02 mL/g rice straw;61.02 mg/g rice straw,能源效益為138.17 kJ/g Polysaccharide。由菌種分析可得知,其菌液主要菌種為Clostridium sporogenes。
摘要 I
Abstract III
Contents V
List of Figures VIII
List of Tables XII
Chapter 1 Introduction 1
1-1 Motivation and objective 1
1-2 Experimental design 3
Chapter 2 Literature review 5
2-1 Hydrolysis 5
2-1-1 Pretreatment of lignocellulosic biomass 5
2-1-2 Physical pretreatment 10
2-1-3 Chemical hydrolysis/pretreatment 11
2-1-4 Physiochemical pretreatment 15
2-1-5 Enzymatic hydrolysis of cellulose andhemicellulose 16
2-2 Enzyme immobilization 19
2-2-1 Physical method of enzyme immobilization 21
2-2-2 Chemical method of enzyme immobilization 23
2-3 Biological hydrogen and ethanol production from Biomass residues 24
2-3-1 Photosynthesis and fermentation 24
2-3-2 Strains Used/Mixed culture and metabolic pathways 26
2-3-3 Different types of feedstock from biomass residues 26
Chapter 3 Material and Methods 29
3-1 Experimental chemicals 29
3-2 Feedstock pretreatment of rice straw 31
3-2-1 Dilute acid pretreatment of rice straw 31
3-2-2 Enzymatic hydrolysis 32
3-3 Enzyme immobilization 32
3-4 Fermentation 33
3-4-1 Seed sludge 33
3-4-2 Experimental Setup 34
3-5 Analytical Methods 36
3-5-1 Total sugar analysis (phenol-sulfuric acid method) 36
3-5-2 Reducing sugar analysis 36
3-5-3 Gas analysis 37
3-5-4 High performance liquid chromatograph analysis 38
3-6 Bacteria community analysis 39
3-6-1 DNA extraction 39
3-6-2 Polymerase chain reaction (PCR) 41
3-6-3 Electrophoresis 42
3-6-4 Denaturing gradient gel electrophoresis (DGGE) 43
3-6-5 Electrophoretic imaging 43
Chapter 4 Results and discussion 44
4-1 Pretreatment of rice straw with dilute hydrochloric acid 44
4-1-1 Effect of concentration of rice straw 44
4-1-2 Effect of rice straw particle size 44
4-1-3 Effect of reaction time 45
4-1-4 Effect of hydrochloric acid and FeCl3 concentrations 46
4-1-5 Enzymatic hydrolysis of dilute acid pretreated rice straw 52
4-2 Enzyme immobilization 55
4-2-1 Different enzyme immobilization matrices tested 55
4-2-2 Effect of calcium chloride concentration on hydrolysis by Ca-alginate immobilized cellulose 58
4-2-3 Effect of initial pH of hydrolyzate on hydrolysis by Ca-alginate immobilized cellulase 60
4-3 Fermentation of hydrolysis rice straw in batch mode and energy production efficiency 62
4-3-1 Preliminary tests on using different concentrations of hydrolyzate for hydrogen production with ethanol 62
4-3-2 Effect of rice straw concentration of hydrogen production with ethanol on batchSSF by immobilized cellulase 73
4-3-3 Effect of initial cultivation pH of hydrogen production with ethanol on batchSSF by immobilized cellulase 78
4-4 Bacteria community analysis 82
4-4-1 Observing bacteria under Scanning Electron Microscope (S3400N) 82
4-4-2 DGGE analysis of Bacteria communities 85
Chapter 5 Conclusions and suggestions 86
5-1 Conclusions 86
5-2 Future Prospect 87
References 88
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