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研究生:洪健庭
研究生(外文):Jian-Ting Hung
論文名稱:氣體飽和溶液包覆苦瓜籽油之降體脂成分以提升其抗氧化性
論文名稱(外文):Co-precipitation of Bitter Gourd Seed Oil and Sucrose Stearate Using Gas Saturated Solutions to Protect α-ESA and Enhance its Oxidation Resistance
指導教授:張傑明
口試委員:楊宏達吳佳娟
口試日期:2015-07-14
學位類別:碩士
校院名稱:國立中興大學
系所名稱:化學工程學系所
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2015
畢業學年度:103
語文別:中文
論文頁數:80
中文關鍵詞:超臨界氣體飽和溶液苦瓜α-eleostearic acid硬脂酸蔗糖酯包覆
外文關鍵詞:particles from gas-saturated solutionbitten melonα-eleostearic acidsucrose stearateencapsulation
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本研究以超臨界氣體飽和溶液(particles from gas-saturated solution, PGSS)製備微粒法,製備硬脂酸蔗糖酯(sucrose stearate, SS)包覆苦瓜籽油(bitter gourd seed oil, BGSO)的微米顆粒。在超臨界 PGSS 共沉澱預備實驗時,藉由改變壓力、溫度、苦瓜籽油與硬脂酸蔗糖酯進料比值,探討對包覆物的產率、單位載藥量及載藥效率。接著進行壓力(120 bar至160 bar)及苦瓜籽油與硬脂酸蔗糖酯進料比值(0.5至1.5)兩變數的應答曲面實驗設計(response surface methodology, RSM),探討兩變數對包覆物的產率、單位載藥量、載藥效率及α-eleostearic acid (α-ESA)回收率所造成的影響。實驗結果顯示包覆物的產率與單位載藥量呈現相反趨勢。RSM 預測 4 個應答值在最適化條件為壓力 120 bar、進料比值 1.5,可獲得預測值為包覆物的產率 45.96%、單位載藥量 25.76%、效率載藥量 69.27%、α-ESA 回收率 29.82%,其與實驗值相近。歸納發現進料比值對包覆物的單位載藥量影響甚大。安定性試驗方面,將 PGSS 包覆物置於室溫 30 天,皆有減緩 α-ESA 濃度之下降。應用 PGSS 製備硬脂酸蔗糖酯包覆苦瓜籽油確實能延緩 α-ESA 之降解,具有保護效果。

關鍵字:超臨界氣體飽和溶液、苦瓜、α-eleostearic acid、硬脂酸蔗糖酯、包覆


In this study the method of supercritical particles from gas-saturated solution (PGSS) has been used for producing the micro-particles of bitter gourd seed oil (BGSO) coated by sucrose stearate (SS). During supercritical PGSS co-precipitation preliminary experiment, total yield, drug content of the coating have been investigated by changing the pressure, temperature, and feed ratio of BGSO and SS. And then the response surface methodology (RSM) experimental design based on the two variables of pressure (120 bar to 160 bar) and the feed ratio (0.5 to 1.5) of BGSO and SS has been implemented to investigate the impacts of the two variables on total yield, drug content, and α-eleostearic acid (α-ESA) recovery rate of the coating. The experimental results indicate the correlation of opposite trend between the total yield and drug content of the coating. The three responses predicted by RSM with optimal conditions of pressure at 120 bar, feed ratio at 1.5 are the total yield of coating at 45.96%, the drug content at 25.76%, and α-ESA recovery rate at 29.82%, all of which are similar to the experimental values. It has been revealed by the induction that the drug content of the coating has been significantly affected by the feed ratio. As for the stability test, keeping the PGSS coating in room temperature for 30 days can slow down the decline of α-ESA concentration. The bitter gourd seed oil coated by sucrose stearate produced by supercritical PGSS can indeed slow down the degradation of α-ESA, which can serve as a protection.
Keywords: particles from gas-saturated solution, bitten melon, α-eleostearic acid, sucrose stearate, encapsulation


摘要 i
Abstract iii
目錄 iv
表目錄 vi
圖目錄 vii
縮寫表 viii
第一章 緒論 1
1-1 研究動機 1
1-2 研究目的與規劃 2
第二章 文獻回顧 3
2-1 苦瓜籽油和 α-eleostearic acid 的性質和功能 3
2-2 超臨界流體萃取技術 5
2-3 超臨界二氧化碳技術與應用 6
2-3-1 超臨界快速膨脹法 8
2-3-2 氣體抗溶劑法 9
2-3-3 氣體飽和溶液製備微粒法 10
2-4 蔗糖酯 11
第三章 實驗方法與步驟 13
3-1 原料與材料 13
3-2 氣體與藥品 13
3-2-1 氣體 13
3-2-2 溶劑與藥品 14
3-2-3 標準品 15
3-3 實驗設備 16
3-3-1 超臨界萃取設備 16
3-3-2 氣相層析儀 18
3-3-3 超臨界抗溶沉澱設備 19
3-3-4 紫外-可見分光光度儀 21
3-3-5 氣體飽和溶液製備微粒設備 22
3-3-6 場發射掃描式電子顯微鏡 24
3-3-7 粒徑分析儀 24
3-3-8 其他設備 24
3-4 實驗方法與步驟 25
3-4-1 種籽前處理 25
3-4-2 超音波萃取 25
3-4-3 索式溶劑萃取 26
3-4-4 超臨界二氧化碳萃取製備苦瓜籽油 26
3-4-5 溶劑對苦瓜籽油與包覆材之溶解度實驗 27
3-4-6 SAS 沉澱包覆程序 28
3-4-7 PGSS 包覆程序 30
3-4-8 樣品分析與定量 31
一、 內標溶液配置 31
二、 甲酯化反應 31
三、 氣相層析儀定量分析 32
四、 包覆產物成分分析 32
3-4-9 α-ESA 儲存安定性 33
3-4-10 粒徑分析 33
3-4-11 電子顯微鏡(SEM)分析 34
第四章 結果與討論 35
4-1 萃取苦瓜籽油 35
4-1-1 索式萃取苦瓜籽油 35
4-1-2 超音波萃取苦瓜籽油 36
4-1-3 超臨界流體萃取與其他萃取比較 36
4-2 苦瓜籽油與包覆材之溶解度實驗 37
4-3 包覆程序預實驗 38
4-3-1 SAS 包覆苦瓜籽油之預實驗 38
1. SAS 包覆程序之壓力探討 38
2. SAS 包覆程序之溫度探討 39
3. SAS 包覆程序之進料比值探討 39
4-3-2 PGSS 包覆苦瓜籽油之預實驗 39
1. PGSS 包覆程序之壓力探討 40
2. PGSS 包覆程序之溫度探討 40
3. PGSS 包覆程序之進料比值探討 41
4-3-3 二種包覆程序之比較 41
4-4 PGSS 之應答曲面實驗設計包覆苦瓜籽油 41
4-4-1 包覆物之產率(TY)應答分析值 42
4-4-2 PGSS 的單位載藥量(DC)應答分析值 42
4-4-3 PGSS 的回收率(R)應答分析值 43
4-4-4 RSM 之預測值與實驗值比較 43
4-5 α-ESA 儲存安定性 44
4-6 粒徑分析 44
4-7 電子顯微鏡(SEM)分析 45
第五章 結論 46
參考文獻 47


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