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研究生:游雅清
研究生(外文):Ya-Ching Yu
論文名稱:小球藻增強紫杉醇引發白血球減少症的恢復力
論文名稱(外文):Chlorella sorokiniana Health Supplement Enhances Recovery from Taxol-Induced Leukopenia
指導教授:林季千
指導教授(外文):Cheng-Chieh Lin
口試委員:陳與國林民昆
口試委員(外文):Yu-Kuo ChenMing-Kuem Lin
口試日期:2015-07-30
學位類別:碩士
校院名稱:國立中興大學
系所名稱:生命科學院碩士在職專班
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2015
畢業學年度:103
語文別:中文
論文頁數:61
中文關鍵詞:紫杉醇成骨細胞
外文關鍵詞:taxolosteoblast
相關次數:
  • 被引用被引用:1
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  • 下載下載:15
  • 收藏至我的研究室書目清單書目收藏:0
小球藻是一種單細胞綠藻,綠藻含有豐富的營養物質,如豐富蛋白質、胺基酸、抗氧化酵素及礦物質等,並可引起多種有益的藥理作用,因此本研究主要是評量小球藻萃取物在改善化療抑制骨髓功能上的能力。模擬老鼠在施與紫杉醇(根據體重A組每公斤17mg,B組每公斤34mg)一天之後7天內,每天皆給予萃取的小球藻精(根據體重每公斤50mg)。在A組第7天與B組第3天接受小球藻精老鼠的末梢血白血球數與紫杉醇治療後老鼠相較雖無顯著差異,但我們也發現接受小球藻精老鼠組的neutrophil皆有顯著升高。在體外試驗顯示,小球藻萃取物能增強顆粒細胞-巨噬細胞與骨髓上的成骨細胞之集落形成能力。此外它還能促進骨髓基質幹細胞分化成脂肪細胞,鹼性磷酸酶陽性成骨細胞與骨組織。而這個結果可能是因為增強核心結合因子(Cbfal)與骨形態發生蛋白(BMP-2)的表現,並同時抑制蝕骨細胞分化因子(ODF)受體。依實驗結果,小球藻萃取物不僅能影響骨髓與成骨細胞分化,且小球藻萃取物對紫杉醇治療老鼠引發的白細胞減少症也會增強它的回復力。

Chlorella sorokiniana (CS) is a single-cell green algae, green algae rich in nutrients, such as protein, amino acids, antioxidant enzymes and minerals. In
addition,Chlorella sorokiniana can also cause a variety of beneficial pharmacological effects.Therefore this study is focused on evaluating the ability Chlorella sorokiniana extract in improving chemotherapy supressed bone marrow function. One day after Taxol treatment, laboratory mice had been given hot water extracted Chlorella every day within following 7 days(Based on subject weight, group A given 17mg/kg, group B given 34mg/kg), laboratory mice had been given hot water extracted Chlorella sorokiniana every day within three weeks (Based on its weight,. 50mg/kg). Compare with mice which received only Taxol treatment, white blood cell counts in peripheral blood which sampled 7 (Group A) and 3 (Group B) days after additional Chlorella extract treatment don''t show significant difference. On the other hand, neutrophil level for both groups had been significantly increased. In vitro assays showed that Chlorella sorokiniana enhanced the colony-forming ability of both granulocyte macrophage colony forming unit (GM-CFU) and osteogenic cells from bone marrow preparations and promoted the differentiation of bone marrow mesenchymal stromal cells into adipocytes, alkaline phosphatase–positive osteoblasts, and bone tissue.his result could be attributed to enhanced expression of Cbfa1 (core binding factor a) and BMP-2 (bone morphogenetic protein) with concurrent suppression of ODF (osteoclast differentiation factor/RANK [receptor activator of NF-κB]) ligand. In summary, Based on results of this experinment, Chlorella extract not only affects bone marrow and osteoblast differentiation but also enhances recovery of mice from Taxol treatment induced leukopenia.

目錄
中文摘要 ⅲ
英文摘要 ⅳ
目錄 ⅶ
圖目錄 ⅸ
表目錄 xi
第一章 緒論 1
1-1研究動機 1
1-2研究目的 2
第二章文獻回顧 3
2-1藻類 3
2-2血液幹細胞調動 16
2-3 TAXOL(紫杉醇) 19
第三章材料與方法 21
3-1 實驗設計 21
3-2實驗材料 23

3-3設備與耗材 24
3-4實驗方法 26
第四章結果 30
4-1血液細胞分析 30
4-2免疫細胞分析 35
4-3小球藻對骨隨細胞的影響 42
4-4 小球藻會影響BMP-2與ODF/RANK配體對成骨細胞MRNA表現 44
第四章 討論 46
參考文獻 49



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