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研究生:劉維慶
研究生(外文):Wei-ChingLiu
論文名稱:藉由結構與功能特性分析蛋白質形變區段
論文名稱(外文):Studying protein conformational change from structural and functional properties
指導教授:張天豪
指導教授(外文):Tien-Hao Chang
學位類別:碩士
校院名稱:國立成功大學
系所名稱:電機工程學系
學門:工程學門
學類:電資工程學類
論文種類:學術論文
論文出版年:2015
畢業學年度:103
語文別:中文
論文頁數:41
中文關鍵詞:結構變化蛋白質結合
外文關鍵詞:conformational changesprotein interaction
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蛋白質的結構變化經常伴隨著蛋白質與其他分子間交互作用一起發生,在交互作用發生前後,蛋白質上的不同區域經常形成大小不同的形變,因此,研究蛋白質結構變化能夠有助於我們觀察蛋白質與分子交互作用的過程,進而更加了解細胞運作的機制。
本研究針對蛋白質交互作用前後的結構變化進行分析,將蛋白質的結構變化區分成大型變區域及小型變區域,再蒐集某些與形變相關的資訊來進行分析及探討,其相關資訊包含:binding target、hydropathy information、order to disorder、disorder to order、order to order、disorder to disorder、phosphorylation sites以及catalytic sites等,利用這八種資訊來探討這些資訊與影響蛋白質結構變化的相關程度,從結果看來,我們發現,在形變較大的區域中,酵素位點與磷酸化位點這兩者因素所含的比例較形變較小的區域來的多,可以推論,這兩者對於形變的影響較其他因素來的重要,除此之外,我們也分析了在大小型變區域中,上述八項因素的分佈情況。

Protein conformational change often occur with interactions by protein and other molecules, after interaction happened, different conformational change may form in each region of protein, since, analyzing the protein conformational change could help us to observe the process of interaction, and we can know more about the operation of cell.
This research analyses the conformational change after interaction, we distinguish each protein into large-conformational-change region (LCC) and small-conformational-change region (SCC), and collect some information related with conformational change to do it, the information include: binding target、hydropathy information、order to disorder、disorder to order、order to order、 disorder to disorder、phosphorylation sites and catalytic sites. We discuss the correlation between protein conformational change and the information mentioned above. According to the result, we found that the rate of catalytic sites and phosphorylation sites in LCC is higher than that in SCC, it may reveal that they are more important than others.
目錄 1
圖目錄 3
表目錄 4
第 一 章 緒論 5
第 二 章 相關研究 7
2.1生物體中的主要分子 7
2.1.1蛋白質 7
2.1.2去氧核醣核酸 9
2.1.3配體 11
2.1.4離子 11
2.2蛋白質的結構轉變 12
2.2.1蛋白質的二級結構 12
2.2.2蛋白質的穩定與非穩定結構 12
2.3相關資料庫 13
2.3.1 蛋白質資料庫 13
2.3.2 分子結構資料庫 13
2.3.3蛋白質結構轉換資料庫 14
第 三 章 資料蒐集與方法 16
3.1產生蛋白質結構對 16
3.2蛋白質形變計算 17
3.3 PDB sites資料蒐集 18
3.4穩定與非穩定的結構轉變 20
3.5親水性/疏水性資料 20
3.6磷酸化位點與酵素位點 21
3.6.1 磷酸化位點(Phosphorylation site) 21
3.6.2 酵素位點(Catalytic site) 21
3.7定義大小形變區域 21
3.7.1 區域最大/最小值(local maximum/minimum) 22
3.7.2 區分形變區域 22
第 四 章 實驗結果與分析 24
4.1區域數量 24
4.2整體資料分佈 26
4.3不同長度中的資料分佈 30
4.4大小形變比較 36
第 五 章 結論與未來展望 38
5.1結論 38
5.2未來展望 38
參考文獻 39

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