跳到主要內容

臺灣博碩士論文加值系統

(44.200.86.95) 您好!臺灣時間:2024/05/20 09:02
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

: 
twitterline
研究生:侯文萍
研究生(外文):Hou, Wen-Ping
論文名稱:海蘊褐藻膠多醣之體外免疫調節作用
論文名稱(外文):In Vitro immunomodulatory Function of Fucoidan from Cladosiphon okamuranus
指導教授:龔瑞林龔瑞林引用關係
指導教授(外文):Kong, Zwe-Ling
口試委員:蘇正德劉興華林培正潘崇良
口試日期:2015-06-15
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:食品科學系
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2015
畢業學年度:103
語文別:中文
論文頁數:48
中文關鍵詞:褐藻膠多醣免疫調節吞噬細胞
外文關鍵詞:Fucoidanimmunomodulatoryphagocytosis
相關次數:
  • 被引用被引用:2
  • 點閱點閱:379
  • 評分評分:
  • 下載下載:63
  • 收藏至我的研究室書目清單書目收藏:0
許多的研究顯示,褐藻中的褐藻膠多醣 (fucoidan) 含有 L-fucose 及硫酸根等基團,具有許多生理活性,如抗腫瘤、免疫調節、抗發炎、抗凝血、抗血栓、抗病毒、抗氧化、降血脂等功能。本研究爲瞭解褐藻膠多醣對於免疫系統之影響,因此以細胞實驗模式探討褐藻膠多醣之免疫調節作用。在體外抗氧化試驗中結果顯示,褐藻膠多醣具有清除自由基能力;而在醣解酵素抑制試驗中,結果顯示褐藻膠多醣具有抑制 α-glucosidase 與 α-amylase 活性能力。在細胞實驗方面以 RAW264.7、Jurkat 及 HB4C5 三株細胞來評估免疫活性並分析其免疫調節機制。實驗結果顯示褐藻膠多醣可以有效的促使以上三株免疫細胞之增生,lipopolysaccharide (LPS)與 RAW 264.7巨噬細胞株共同培養下具有良好抗發炎效果,降低 Nitro-Blue tetrazolium chloride (NBT) 產生,抑制 TNF-α、IL-6,並促使細胞激素 IL-10 分泌,也可提升巨噬細胞吞噬活性;對人類融合瘤細胞株 HB4C5 可以促使其分泌抗體 IgM,未顯著促使分泌IgG抗體;有效促使 Jurkat分泌 IFN-γ。綜合上述結果顯示褐藻膠多醣具有體外調節免疫之作用。
Fucoidan refers to a type of polysaccharide which contains substantial percentages of L-fucose and sulfate ester groups. It has many physiological activities such as anti-tumor, immunomodulatory, anti-inflammatory, anti-clotting, anti-thrombotic, anti-viral, anti-oxidation, and anti-lipemia. In this study, fucoidan isolated from Cladosiphon okamuranus investigated for its immunomodulatory potential by using in vitro assays. The glycosidase inhibitory assay showed that fucoidan has the ability to inhibit α-glucosidase and α-amylase. In vitro, fucoidan has the ability to scavenge free radicals. The immune-stimulating activity of fucoidan was elucidated by using RAW264.7, Jurkat and HB4C5 cells. The result showed that fucoidan enhanced proliferation of RAW 264.7 cells under the influence of significantly decreased the level of Nitro-Blue tetrazolium chloride (NBT), restrained the secretion of IL-6 and TNF-α, stimulated production of IL-10 and also enhanced phagocytosis activity of RAW264.7. Fucoidan also enhanced HB4C5 cell proliferation and significantly stimulated secretion of IgM and does not affect the IgG, as well as stimulated interferon-γ production of Jurkat cell. Based on these results, fucoidan has a potential to be used as a natural reagent for immune regulation.
摘要 IV
Abstract V
目錄 VI
名詞縮寫 X
壹、緒論 1
貳、文獻回顧 2
一、褐藻膠多醣(Fucoidan)之簡介 2
1.1 藻類簡介 2
1.2 海藻多醣定義 2
1.3 Fucoidan 2
1.4 Fucoidan來源及各結構 3
1.5 褐藻膠多醣之生理功效 6
1.5-1 抗癌、抗腫瘤 6
1.5-2 調節免疫 7
1.5-3 抗發炎作用 7
1.5-4 抗氧化作用 7
1.5-5 抗凝血活性 8
1.5-6 抗血栓活性 8
1.5-7 抗病毒作用 8
1.5-8 預防高血脂症 9
1.5-9 保護肝臟 9
1.5-10 表皮細胞保護與修復 9
二、免疫調節系統 10
2.1 免疫細胞的活化 10
2.2-1 T細胞的活化 10
2.2-2 吞噬細胞的活化 11
2.2-3 自然殺手細胞之作用及活性 11
2.3 免疫反應的調節機制 12
2.3-1 發炎反應 12
2.3-2 細胞激素 (Cytokines) 12
2.3-3 IFN-γ 12
2.3-4 NF-κB 12
三、免疫調節功能評估方法 13
叁、整體實驗設計 14
肆、實驗材料與方法 15
一、實驗材料 15
1.1 褐藻膠多醣樣品 15
1.2 細胞株 15
1.3 藥品 15
1.3-1 細胞培養 15
1.3-2 總醣含量測定 15
1.3-3 DPPH自由基清除測定 16
1.3-4 細胞存活率測定 (MTT assay) 16
1.3-5 超氧陰離子測定 (NBT) 16
1.3-6 吞噬細胞活性分析 16
1.4 設備 16
二、實驗方法 17
2.1 樣品分析 17
2.1-1 總醣含量測定 17
2.1-2 硫酸根分析 17
2.2 抗氧化活性分析 17
DPPH自由基清除能力之測定 17
2.3 醣解酵素抑制活性之測定 18
2.3-1 α-glucosidase抑制作用之測定 18
2.3-2 α-amylase 抑制作用之測定 18
2.4 細胞實驗 18
2.4-1 懸浮性細胞培養 18
2.4-2 附著性細胞培養 19
2.4-3 細胞存活率 19
2.4-4 抗體產量測定 (ELISA assay) 19
2.4-5 RAW264.7細胞激素分泌測定 20
2.4-6 Jurkat細胞激素分泌測定 20
2.4-7 超氧陰離子測定 (NBT reducting assay) 20
2.4-8 吞噬細胞活性分析 21
三、統計分析 21
伍、結果與討論 22
一、褐藻膠多醣總醣含量及硫酸根分析 22
1. 總醣含量測定 22
2. 硫酸根分析 22
二、褐藻膠多醣抗氧化活性分析 22
DPPH 自由基清除能力 22
三、褐藻膠多醣對抑制α-glucosidase和α-amylase之活性之影響 22
四、以體外實驗探討褐藻膠多醣對於細胞免疫活性之影響 23
1. 褐藻膠多醣對細胞存活率探討 23
2. 褐藻膠多醣對胞內超氧陰離子產生之影響 23
3. 褐藻膠多醣對RAW264.7細胞細胞激素分泌試驗 (ELISA assay) 23
4. 褐藻膠多醣對RAW264.7細胞株吞噬活性之影響 24
5. 褐藻膠多醣對HB4C5 細胞抗體產量試驗 (ELISA assay) 24
6. 褐藻膠多醣對Jurkat細胞細胞激素分泌試驗 (ELISA assay) 24
陸、結論 25
柒、參考文獻 26
捌、圖表 36

王升陽。2011。你所不知道的牛樟芝。蘋果屋出版社。新北市,臺灣。
王彥容。2007。重緣葉馬尾藻海藻多醣類及其奈米粒抗細胞增生效果之探討。國立台灣海洋大學食品科學系碩士學位論文。基隆,臺灣。
王莉瑩。2013。馬尾藻岩藻多醣之免疫調節功能評估。國立臺灣海洋大學食品科學系碩士學位論文。基隆,臺灣。
方季堂。1995。鮑魚萃取物對人體免疫細胞活化因子的純化與特性研究。國立臺灣海洋大學水產食品科學系碩士學位論文。基隆,臺灣。
江仁淳。2001。臺灣重緣葉馬尾藻海藻多醣體之免疫調節功能評估。國立臺灣海洋大學食品科學系碩士學位論文。基隆,臺灣。
行政院衛生署。2002。健康食品之免疫調節功能評估方法。臺北,臺灣。
行政院衛生署。2006。健康食品管理法。臺北,臺灣。
行政院衛生福利部。2014。民國102年台灣國人主要死因統計資料。行政院衛福部統計室。臺北,臺灣。
林明泉。2000。臨床血清免疫學。藝軒圖書出版社。新北市,臺灣。
柯慧君。2008。烏苓参萃取物之免疫調節功效研究。大仁科技大學生物科技研究所碩士學位論文。屏東,臺灣。
徐筱玫。2012。海藻多醣之硫酸基含量及分子量大小對小鼠巨噬細胞 RAW 264.7 抗發炎活性之影響。國立臺灣海洋大學食品科學系碩士學位論文。基隆,臺灣。
陳怡穎。2003。台灣土肉桂葉精油脂組成與抗氧化性。國立臺灣大學食品科技研究所碩士學位論文。臺北,臺灣。
黃淑芳。1989。認識藻類。台灣省立博物館出版。臺北,臺灣。
Abdel-Fattah AF, Hussein MM-D, Fouad ST. 1978. Carbohydrates of the brown seaweed Dictyota dichotoma. Phytochemistry 17(4):741-3.
Aisa Y, Miyakawa Y, Nakazato T, Shibata H, Saito K, Ikeda Y, Kizaki M. 2005. Fucoidan induces apoptosis of human HS-sultan cells accompanied by activation of caspase-3 and down-regulation of ERK pathways. American Journal of Hematology 78(1):7-14.
Albuquerque IRL, Queiroz KCS, Alves LG, Santos EA, Leite EL, Rocha HAO. 2004. Heterofucans from Dictyota menstrualis have anticoagulant activity. Brazilian Journal of Medical and Biological Research 37(2):167-171.
Alves AP, Mulloy B, Diniz JA, Mourao PAS. 1997. Sulfated polysaccharides from the egg jelly layer are species-specific inducers of acrosomal reaction in sperms of sea urchins. Journal of Biological Chemistry 272(11):6965-6971.
Alves AP, Mulloy B, Moy GW, Vacquier VD, Mourao PAS. 1998. Females of the sea urchin Strongylocentrotus purpuratus differ in the structure of their egg jelly sulphated fucans. Glycobiology 8(9):939-946.
Anno K, Terahata H, Hayashi Y, Seno N, 1966. Isolation and purification of fucoidin from brown seaweed Pelvetia wrightii. Agricultural and Biological Chemistry 30(5):495-499.
Apostolidis E, Kwon YI, Shetty K. 2007. Inhibitory potential of herb, fruit, and fungal-enriched cheese against key enzymes linked to type 2 diabetes and hypertension. Innovative Food Science and Emerging Technologies 8(1):46-54.
Azuma M, Cayabyab M, Buck D, Phillips JH, Lanier LL. 1992. Involvement of CD28 in MHC-unrestricted cytotoxicity mediated by a human natural killer leukemia cell line. Journal of Immunology 149(4):1115-1123.
Baeuerle PA, Henkel T. 1994. Function and activation of NF-kappa B in the immune system. Annual Review of Immunology 12:141-79
Baeuerle PA, Baltimore D. 1996. NF-kappa B: Ten years after. Cell 87(1):13-20.
Benbernou N, Esnault S, Shin HC, Fekkar H, Guenounou M. 1997. Differential regulation of IFN-gamma, IL-10 and inducible nitric oxide synthase in human T cells by cyclic AMP-dependent signal transduction pathway. Immunology 91(3), 361-368.
Berkower I, Kawamura H, Matis LA, Berzofsky JA. 1985. T cell clones to two major T cell epitopes of myoglobin: effect of I-A/I-E restriction on epitope dominance. Journal of Immunology 135(4):2628-2634.
Berteau O, Mulloy B. 2003. Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharide. Glycobiology 13(6):29R-40R.
Black WAP, 1954. The seasonal variation in the combined L-fucose content of the common British laminariaceae and fucaceae. Journal of the Science of Food and Agriculture 5(9):445-448.
Chizhov AO, Dell A, Morris HR, Haslam SM, McDowell RA, Shashkov AS, Nifant'ev NE, Khatuntseva EA, Usov AI. 1999. A study of fucoidan from the brown seaweed Chorda filum. Carbohydrate Research 320(1-2):108-119.
Choi EM, Koo SJ, Hwang JK. 2004. Immune cell stimulating activity of mucopolysaccharide isolated from yam (Dioscorea batatas). Journal of Ethnopharmacology 91(1):1-6.
Cippitelli M, Sica A, Viggiano V, Ye J, Ghosh P, Birrer MJ, Young HA. 1995. Negative transcriptional regulation of the interferon-gamma promoter by glucocorticoids and dominant negative mutants of c-Jun. Journal of Biological Chemistry 270(21):12548-12556.
Colliec-Jouault S, Millet J, Helley D, Sinquin C, Fischer AM. 2003. Effect of low-molecular-weight fucoidan on experimental arterial thrombosis in the rabbit and rat. Journal of Thrombosis and Haemostasis 1(5):1114-1115.
Costa LS, Fidelis GP, Telles CB, Dantas-Santos N, Camara RB, Cordeiro SL, Costa MS, Almeida-Lima J, Melo-Silveira RF, Oliveira RM, Albuquerque IR, Andrade GP, Rocha HA. 2011. Antioxidant and antiproliferative activities of heterofucans from the seaweed Sargassum filipendula. Marine Drugs 9(6):952-66.
Coughlan S. 1977. Sulfate uptake in Fucus serratus. Journal of Experimental Botany 28(5):1207-1215.
Crayton M A, Wilson E, Quatrano RS. 1974. Sulfation of fucoidan in Fucus embryosⅡ. Seperation from initiation of polar growth. Developmental Biology 39(1):164-167.
Cumashi A, Ushakova NA, Preobrazhenskaya ME, D'InceccoA, Piccoli A, Totani L, Tinari N, Morozevich GE, Berman AE, Bilan MI, Usov AI, Ustyuzhanina NE, Grachev AA, Sanderson CJ, Kelly M, Rabinovich GA, Iacobelli S, Nifantiev N. E. 2007. A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds. Glycobiology 17(5):541-552.
Curnutte JT, Babior BM. 1987. Chronic granulomatous disease. Advances in Human Genetics 16(0):229-297.
Delgado M, Ganea D. 2000. Inhibition of IFN-gamma-induced janus kinase-1-STAT1 activation in macrophages by vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide. Journal of Immunology 165(6):3051-3057.
de Reviers B, Mabeau S, Kloareg B. 1983. Essai d'interpr_etation de la structure des fucoidanes en liaison avec leur localisation dans la paroi des pheophycees. Cryptogamie Algologie 4(1-2):55-62.
Ding Q, Jin T, Wang Z, Chen Y. 2007. Catalase potentiates retinoic acid-induced THP-1 monocyte differentiation into macrophage through inhibition of peroxisome proliferator-activated receptor gamma. Journal of Leukocyte Biology 81(6):1568-1576.
Dobashi K, Nishino T, Fujihara M, Nagumo T. 1989. Isolation and preliminary characterization of fucose-containing sulfated polysaccharides with blood-anticoagulant activity from the brown seaweed Hizikia fusiforme. Carbohydrate Research 194:315-320.
Duarte MER, Cardoso MA, Noseda MD, Cerezo AS. 2001. Structural studies on fucoidans from the brown seaweed Sargassum stenophyllum. Carbohydrate Research 333(4):281-293.
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28(3):350-356.
Durand E, Helley D, Zen A, Dujols C, Bruneval P, Colliec-Jouault S, Fischer AM, Lafont A. 2008. Effect of low molecular weight fucoidan and low molecular weight heparin in a rabbit model of arterial thrombosis. Journal of Vascular Research 45(6):529-537.
Falasca GF, Ramachandrula A, Kelley KA, O'Connor CR, Reginato AJ. 1993. Superoxide anion production and phagocytosis of crystals by cultured endothelial cells. Arthritis and Rheumatism 36(1):105-116.
Farrar MA, Schreiber RD. 1993. The molecular cell biology of interferon-gamma and its receptor. Annual Review of Immunology 11:571-611.
Fennema OR. 1985. Food Chemistry, 2nd ed., revised and expanded. Marcel Dekker, Inc., New York.
Ferrari M, Fornasiero MC, Isetta AM. 1990. MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. Journal of Immunological Methods 131(2):165-172.
Foxwell BM, Willcocks JL, Taylor-Fishwick DA, Kulig K, Ryffel B, Londei M. 1993. Inhibition of activation-induced changes in the structure of the T cell interleukin-7 receptor by cyclosporin A and FK506. European Journal of Immunology 23(1):85-89.
Franzoso G, Carlson L, Poljak L, Shores EW, Epstein S, Leonardi A, Grinberg A, Tran T, Scharton-Kersten T, Anver M, Love P, Brown K, &; Siebenlist U. 1998. Mice deficient in nuclear factor (NF)-kappa B/p52 present with defects in humoral responses, germinal center reactions, and splenic microarchitecture. Journal of Experimental Medicine 187(2):147-159.
Fried SK, Russell CD, Grauso NL, Brolin RE. 1993. Lipoprotein lipase regulation by insulin and glucocorticoid in subcutaneous and omental adipose tissues of obese women and men. Journal of Clinical Investigation 92(5):2191-2198.
Giraux JL, Tapon-Bretaudiere J, Matou S, Fischer AM. 1998. Fucoidan, as heparin, induces tissue factor pathway inhibitor release from cultured human endothelial cells. Thrombosis and Haemostasis 80(4):692-695.
Grauffel V, Kloareg B, Mabeau S, Durand P, Jozefonvicz J. 1989. New natural polysaccharides with potent antithrombic activity: Fucans from brown algae. Biomaterials 10(6):363-368.
Halvorson H, Ellias L. 1958. The purification and properties of an α-glucosideof Saccharomyces italicus Y1225. Biochimica et Biophysica Acta 30(1):28-40.
Hanada T, Yoshimura A. 2002. Regulation of cytokine signaling and inflammation. Cytokine and Growth Factor Reviews 13(4-5): 413-421.
Heo SJ, Park EJ, Lee KW, Jeon YJ. 2005. Antioxidant activities of enzymatic extracts from brown seaweeds. Bioresource Technology 96(14):1613-1623.
Heo SJ, Hwang JY, Choi JI, Han JS, Kim HJ, Jeon YJ. 2009. Diphlorethohydroxycarmalol isolated from Ishige okamurae, a brown algae, a potent α-glucosidase and α-amylase inhibitor, alleviates postprandial hyperglycemia in diabetic mice. European Journal of Pharmacology 615(1-3):252-256.
Hwang PA, Wu CH, Gau SH, Chien SY, Hwang DF. 2010. Antioxidant and immune-stimulating activities of hot-water extract from seaweed Sargassum hemiphyllum. Journal of Marine Science and Technology 18(1)41-46.
Jiang Z, Hama Y, Yamaguchi K, Oda T. 2012. Inhibitory effect of sulphated polysaccharide porphyran on nitric oxide production in lipopolysaccharide-stimulated RAW264.7 macrophages. Journal of Biochemistry 151(1):65-74.
Killing H. 1913. Zur biochemie der Meersalgen. Hoppe-Seyler's Zeitschrift für Physiologische Chemie 83(3):171-197.
Kim KY, Nam KA, Kurihara H, Kim SM. 2008. Potent α-glucosidase inhibitors purified from the red alga Grateloupia elliptica. Phytochemistry 69(16):2820-2825.
Kim WJ, Koo YK, Jung MK, Moon HR, Kim SM, Synytsya A, Yun-Choi HS, Kim YS, Park JK, Park YI. 2010. Anticoagulating activities of low-molecular weight fuco-oligosaccharides prepared by enzymatic digestion of fucoidan from the sporophyll of Korean Undaria pinnatifida. Archives of Pharmacal Research 33(1):125-131.
Kitamura K, Matsuo M, Yasui T. 1992. Enzymic degradation of fucoidan by fucoidanase from the hepatopancreas of Patinopecten yessoensis. Bioscience, Biotechnology, and Biochemistry 56(3):490-494.
Kumalasari ID, Harmayani E, Lestari LA, Raharjo S, Asmara W, Nishi K, Sugahara T. 2012. Evaluation of immunostimulatory effect of the arrowroot (Maranta arundinacea. L) in vitro and in vivo. Cytotechnology 64(2):131-7.
Lee D.S, Lee SH. 2001. Genistein, a soy isoflavone, is a potent α-glucosidase inhibitor. FEBS Letters 501(1):84-86.
Lee KH, Bae SW, Cho CH, Rhee KH. 2009. Fucoidan protects human skin fibroblast cell line HS68 against γ-radiation-induced damage. The Open Natural Products Journal 2:38-41.
Lehere RI, Ganz T, Selsted ME, Babior BM, Curntte JT. 1988. Neutrophils and host defense. Annals of Internal Medicine 109(2):127-142.
Leite EL, Medeiros MGL, Rocha HAO, Farias GGM, daSilva LF, Chavante SF, de Abreu LD, Dietrich CP, Nader HB. 1998. Structure and pharmacological activities of a sulfated xylofucoglucuronan from the alga Spatoglossum schroederi. Plant Science 132:215-228.
Li B, Lu F, Wei X, Zhao R. 2008. Fucoidan: Structure and bioactivity. Molecules 13(8):1671-1695.
Lu Y, Foo Y. 2000. Antioxidant radical scavenging activies of polyphenols from apple pomaceae. Food Chemistry 68(1):81-85.
Mabeau S, Kloareg B, Joseleau JP. 1990. Fractionation and analysis of fucans from brown algae. Phytochemistry 29(8):2441-2445.
Maruyama H, Nakajima J, Yamamoto I. 1987. A study on the anticoagulant and fibrinolytic activities of a crude fucoidan from the edible brown seaweed Laminaria religiosa, with special reference to its inhibitory effect on the growth of sarcoma-180 ascites cells subcutaneously implanted into mice. Kitasato Archives of Experimental Medicine 60(3):105-121.
Matsui T, Tanaka T, Tamura S, Toshima A, Miyata Y, Tanaka K, Matsumoto K. 2007. α-glucosidase inhibitory profile of catechins and theaflavins. Journal of Agricultural and Food Chemistry 55(1):99-105.
Meng Y, Zou QW, Bai JY, Cheng GF, Xiao X, Zhang S, Zhu XY, Wang XL. 2014. Effect of artificial musk aqueous extract on the expressions of inflammatory mediators released from lipopolysaccharide-stimulated RAW264.7 cells. Chinese Academy of Medical Sciences 36(6):583-586.
Mian AJ, Percival E. 1973. Carbohydrates of the brown seaweeds himanthalia lorea, bifurcaria bifurcata, and Padina pavonia : Part I. extraction and fractionation. Carbohydrate Research 26(1):133-46.
Minami, Y., T. Kono, T. Miyazaki and T. Taniguchi. 1993. The IL-2 receptor complex: its structure, function, and target genes. Annual Review of Immunology 11:245-268.
Minato N, Reid L, Bloom BR. 1981. On the heterogeneity of murine natural killer cells. Journal of Experimental Medicine 154(3):750-762.
Mori H, Nisizawa K. 1982. Sugar constituents of sulfated polysaccharides from the fronds of Sargassum ringgoldianum. Nippon Suisan Gakkaishi 48(7):981-986.
Mori H, Kamei H, Nishide E, Nisizawa K. 1982. Sugar constituents of some sulfated polysaccharides from the sporophylls of wakame (Undaria pinnatifida) and their biological activities. Marine Algae in Pharmaceutical Science 2:109-121.
Mourão PAS, Bastos IG. 1987. Highly acidic glycans from sea cucumbers. European Journal of Biochemistry 166(3):639-645.
Mulloy B, Ribeiro AC, Alves AP, Vieira RP, Mourao PAS. 1994. Sulfated fucans from echinoderms have a regular tetrasaccharide repeating unit defined by specific patterns of sulfation at the O-2 and O-4 positions. Journal of Biological Chemistry 269(35):22113-22123.
Nagaoka M, Shibata H, Kimura-Takagi I, Hashimoto S, Kimura K.,Makino T, Aiyama R, Ueyama S, Yokokura T. 1999. Structural study of fucoidan from Cladosiphon okamuranus Tokida. Glycoconjugate Journal 16(1):19-26.
Nick JA, Avdi NJ, Gerwins P, Johnson GL, Worthen GS. 1996. Activation of a p38 mitogen-activated protein kinase in human neutrophils by lipopolysaccharide. Journal of Immunology 156(12):4867-4875.
Nishino T, Nagumo T. 1991.Structural characterization of a new anticoagulant fucan sulfate from the brown seaweed Ecklonia kurome. Carbohydrate Research 211(1): 77-90.
Percival EGV, Ross AG. 1950. The constitution of laminarin. Part II. The soluble laminarin of Laminaria digitata. Journal of the Chemical Society 720-726.
Percival E, McDowell, RH. 1967. Chemistry and enzymology of marine algal polysaccharides. London Academic Press 157-164.
Pereira MS, Mulloy B, Mourao PA. 1999. Structure and anticoagulant activity of sulfated fucans. Comparison between the regular, repetitive, and linear fucans from echinoderms with the more heterogeneous and branched polymers from brown algae. Journal of Biological Chemistry 274(12):7656-7667.
Ponce NMA, Pujol CA, Damonte EB, Flores ML, Stortz CA. 2003. Fucoidans from the brown seaweed Adenocystis utricularis: extraction methods, antiviral activity and structural studies. Carbohydrate Research 338(2):153-165.
Preeprame S, Hayashi K, Lee JB, Sankawa U, Hayashi T. 2001. A novel antivirally active fucan sulfate derived from an edible brown alga, Sargassum horneri. Chemical and Pharmaceutical Bulletin 49(4):484-485.
Qiu XD, Amarasekara A, Doctor V. 2006. Effect of oversulfation on the chemical and biological properties of fucoidan. Carbohydrate Polymers 63(2):224-228.
Ribeiro AC, Vieira RP, Mourao PAS, Mulloy B. 1994. A sulfated α-L-fucan from sea cucumber. Carbohydrate Research 255:225-240.
Roberts AM, Ovechkin AV, Mowbray JG, Robinson TW, Lominadze D. 2004. Effects of pulmonary ischemia-reperfusion on platelet adhesion in subpleural arterioles in rabbits. Microvascular Research 67(1):29-37.
Rocha de Souza MC, Marques CT, Guerra Dore CM, Ferreira da Silva FR, Oliveira Rocha HA, Leite EL. 2007. Antioxidant activities of sulfated polysaccharides from brown and red seaweeds. Journal of Applied Phycology 19(2):153-160.
Rozkin MY, Levina MN, Kameneva NS, Usov AI, Yefimov VS. 1989. Investigation of the mechanism of the anticoagulant action of fucoidans. Farmakologiia I Toksikologiia 3:48-51.
Saito A, Yoneda M, Yokohama S, Okada M, Haneda M, Nakamura K. 2006. Fucoidan prevents concanavalin A-induced liver injury through induction of endogenous IL-10 in mice. Hepatology Research 35(3):190-198.
Sanceau J, Hiscott J, Delattre O, Wietzerbin J. 2000. IFN-beta induces serine phosphorylation of Stat-1 in Ewing's sarcoma cells and mediates apoptosis via induction of IRF-1 and activation of caspase-7. Oncogene 19(30):3372-3383.
Schwacha MG. 2003. Macrophages and post-burn immune dysfunction. Burns 29(1):1-14.
Schweiger RG. 1962. Methanolysis of fucoidan. Ⅰ. Preparation of methyl α-L-fucose and L-fucose. Journal of Organic Chemistry 27(12):4267-4272.
Silva TMA, Alves LG, de Queiroz KCS, Santos MGL, Marques CT, Chavante SF, Rocha HAO, Leite EL. 2005. Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora. Brazilian Journal of Medical and Biological Research 38(4):523-533.
Tanaka K, Sorai S. 1970. Hydrolysis of fucoidan by abalone liver α-L-fucosidase. FEBS Letters 9(1):45-48.
Teng FY, Wu SK, Hsia YA, Kao WB, Hsieh YD. 2006. Modulation of inflammation, apoptosis, and oncogenesis by the nuclear transcription factor, NF-κB. Chinese Journal of Dentistry 25(1):12-24.
Tenzaki T, Ishiguro T. 1989. Response of human lymphocytes to phytohemagglutinin (PHA) as measured by fluorescence polarization technique--differences between malignant conditions and benign or healthy conditions. Nihon Gan Chiryo Gakkai Shi, 24(4):817-825
Terho TT, Hartiala K. 1971. Method for determination of the sulfate content of glycosaminoglycans. Analytical Biochemistry 41(2):471-476.
Teruya T, Konishi T, Uechi S, Tamaki H, Tako M. 2007. Anti-proliferative activity of oversulfated fucoidan from commercially cultured Cladosiphon okamuranus TOKIDA in U937 cells. International Journal of Biological Macromolecules 41(3):221-226.
Thomes P, Rajendran M, Pasanban B, Rengasamy R. 2010. Cardioprotective activity of Cladosiphon okamuranus fucoidan against isoproterenol induced myocardial infarction in rats. Phytomedicine 18(1):52-57.
Trinchero J, Ponce NM, Cordoba OL, Flores ML, Pampuro S, Stortz CA, Salomon H, Turk G. 2009. Antiretroviral activity of fucoidans extracted from the brown seaweed Adenocystis utricularis. Phytotherapy Research 23(5):707-712.
Trinchieri G. 1989. Biology of natural killer cells. Advances in Immunolgy 47:187-376.
Tsuchiya S, Kobayashi Y, Goto Y, Okumura H, Nakae S, Konno T, Tada K. 1982. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Research 42(4):1530-1536.
Usov AI, Smirnova GP, Klochkova NG. 2001. Algae polysaccharides. 55. Polysaccharide composition of some brown Kamchatka algae. Bioorganicheskaia Khimiia 27(6):444-448.
Usui T, Asari K, Mizuno T. 1980. Isolation of highly purified fucoidan from Eisenia bicyclis and its anticoagulant and antitumor activities. Agricultural and Biological Chemistry 44(8):1965-1966.
Vilela-Silva AC, Alves AP, Valente AP. 1999. Structure of the sulfated α-L-fucan from the egg jelly coat of the sea urchin Strongylocentrotus franciscanus: Patterns of preferential 2-O-and 4- O-sulfation determine sperm cell recognition. Glycobiology 9(9):927-933.
Vilela-Silva AC, Castro MO, Valente AP, Biermann CH, Mourao PAS. 2002. Sulfated fucans from the egg jellies of the closely related sea urchins Strongylocentrotus droebachiensis and Strongylocentrotus pallidus ensure species-specific fertilization. Journal of Biological Chemistry 277(1):379-387.
Wang WT, Zhou JH, Xing ST, Guan HS. 1994. Immunomodulating action of marine algae sulfated polysaccharides on normal and immunosuppressed mice. Chinese Journal of Pharmacology and Toxicology 8(3):199-202.
Wort DJ. 1955. The seasonal variation in chemical composition of Macrocystis integrifolia and Neroecystis luetkeana in British Colombia coastal waters. Canadian Journal of Botany 33(4):323-340.
Wu X, Yang M, Huang X, Yan J, Luo Q. 2003. Effect of fucoidan on splenic lymphocyte apoptosis induced by radiation. Chinese Journal of Radiological Medicine and Protection 23(6):430-432.
Wu X, Yang M, Wuang X, Yan J, Luo Q. 2004. Effect of Laminaria japonica polysaccharides on radioprotection and splenic lymphocyte apoptosis. Medical Journal of Wuhan University 25(3):239-241
Yamamoto I, Takahashi M, Tamura E, Maruyama H. 1982. Antitumor activity of crude extracts from edible marine algae against L-1210 leukemia. Botanica Marina 25(9): 455-457.
Yang JW, Yoon SY, Oh SJ, Kim SK, Kang KW. 2006. Bifunctional effects of fucoidan on the expression of inducible nitric oxide synthase. Biochemical and Biophysical Research Communications 346(1):345-350.
Yang M, Ma C, Sun J, Shao Q, Gao W, Zhang Y, Li Z, Xie Q, Dong Z, Qu X. 2008. Fucoidan stimulation induces a functional maturation of human monocyte-derived dendritic cells. International Immunopharmacology 8(13-14):1754-1760.
Ye J, Li Y, Teruya K, Katakura Y, Ichikawa A, Eto H, Hosoi M, Hosoi M, Nishimoto S, Shirahata S. 2005. Enzyme-digested fucoidan extracts derived from seaweed Mozuku of Cladosiphon novae-caledoniae kylin inhibit invasion and angiogenesis of tumor cells. Cytotechnology 47(1-3):117-126.
Ye J, Ortaldo JR, Conlon K, Winkler-Pickett R, Young HA. 1995. Cellular and molecular mechanisms of IFN-gamma production induced by IL-2 and IL-12 in a human NK cell line. Journal of Leukocyte Biology 58(2):225-233.
Yokota T, Nagashima M, Ghazizadeh M, Kawanami O. 2009. Increased effect of fucoidan on lipoprotein lipase secretion in adipocytes. Life Sciences 84(15-16):523-529.
Zhang QB, Yu PZ, Zhou GF, Li ZE, Xu ZH. 2003. Studies on antioxidant activities of fucoidan from Laminaria japonica. Chinese Traditional Herbal Drugs, 34(9):824-826.

連結至畢業學校之論文網頁點我開啟連結
註: 此連結為研究生畢業學校所提供,不一定有電子全文可供下載,若連結有誤,請點選上方之〝勘誤回報〞功能,我們會盡快修正,謝謝!
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊