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研究生:林筱蓁
研究生(外文):Lin, Xiao-Zhen
論文名稱:褐藻醣膠結合順鉑的奈米粒子之製備及其特性
論文名稱(外文):The fabrication and characterization of fucoidan-cisplatin conjugate nanoparticles
指導教授:許富銀
指導教授(外文):Hsu, Fu-Yin
口試委員:蔡曉雯黃培安
口試委員(外文):Tsai, Shiao-WenHawng, Pei-An
口試日期:2015-07-27
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:生命科學暨生物科技學系
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2015
畢業學年度:103
語文別:中文
論文頁數:40
中文關鍵詞:褐藻醣膠順鉑化療免疫奈米粒子
外文關鍵詞:FucoidanCisplatinChemotherapyImmuneNanoparticles
相關次數:
  • 被引用被引用:0
  • 點閱點閱:400
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  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
褐藻醣膠是一種硫酸化的多醣並且被證實有許多的免疫調節功能,如:促進T細胞活化,增強抗腫瘤反應,誘導癌細胞走向凋亡路徑等,更可以抑制使癌細胞的藥物外排幫浦進而減少癌細胞的多重抗藥性。順鉑為一種臨床腫瘤治療中廣泛使用的化療藥物。Thakral等人研究指出,鉑(II)原子與陰離子聚合物的負電荷相互作用可能會導致自發性的摺疊,以形成奈米複合物。因此,本研究的目的為製備褐藻醣膠結合順鉑的奈米粒子。所合成的褐藻醣膠-順鉑奈米粒子(FuCis)的粒徑大小介於150到600 nm之間。並且體外細胞實驗顯示FuCis奈米粒子能夠增強免疫細胞活性並且具有良好的化療效果。
Fucoidan is a sulfated polysaccharide and has been demonstrated having a variety of immunomodulatory effects, such as promoting activation of T cells and enhancing anti-tumor responses, inhibit cancer cell drug efflux pump of multidrug resistance. Cisplatin is a widely used as chemotherapy drug in the treatment of human solid tumors. Thakral et al. showed that the interaction of the platinum (II) atom with the negative charge of the anion polymer could cause spontaneous folding to form a nano-conjugate. Hence, the aim of this study was to fabricate the fucoidan-cisplatin conjugate nanoparticles. The diameter of fucoidan-cisplatin conjugate nanoparticles (FuCis) was between 150 and 600 nm. Moreover, the FuCis could enhance the immune activity and reveal a better chemotherapeutic efficiencies in vitro.
目錄
1 前言 - 1 -
1.1 Cisplatin順鉑 - 2 -
1.2 EPR效應(Enhanced Permeability and Retention, EPR Effect) - 3 -
1.3 藥物運送系統(Drug-delivery systems, DDS) - 4 -
1.4 多醣藥物載體 - 4 -
1.5 褐藻醣膠 (Fucoidan) - 5 -
1.6 多重抗藥性 (Multidrug resistance, MDR) - 6 -
2 研究動機與目的 - 7 -
3 材料與方法 - 8 -
3.1 材料 - 8 -
3.1.1 藥品 - 8 -
3.1.2 耗材與儀器 - 9 -
3.1.3 細胞株 - 10 -
3.2 方法 - 11 -
3.2.1 FuCis奈米粒子製備 - 12 -
3.2.2 Cisplatin濃度分析 - 12 -
3.2.3 細胞實驗 - 13 -
3.2.4 統計分析方法 - 13 -
4 結果與討論 - 14 -
4.1 FuCis奈米粒子之大小分析 - 14 -
4.2 Cisplatin 濃度分析 - 22 -
4.3 細胞實驗 - 28 -
5 結論 - 34 -
參考文獻: - 35 -


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