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研究生:柯雅青
研究生(外文):Ya-Ching Ko
論文名稱:臺灣檫樹之遺傳保育學
論文名稱(外文):Genetic Conservation of Sassafras randaiense
指導教授:鍾國芳鍾國芳引用關係
指導教授(外文):Kuo-Fang Chung
口試委員:江友中廖培鈞
口試委員(外文):Yu-Chung ChiangPei-Chun Liao
口試日期:2013-07-23
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:森林環境暨資源學研究所
學門:農業科學學門
學類:林業學類
論文種類:學術論文
論文出版年:2015
畢業學年度:103
語文別:中文
論文頁數:72
中文關鍵詞:樟科微衛星體親緣地理近交衰退物種分布模擬
外文關鍵詞:LauraceaeInbreeding depressionmicrosatellitephylogeograpyspecies distribution model
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樟科檫樹屬全世界僅三種,為北美東部—東亞間斷分布的代表類群。臺灣檫樹為臺灣特有種,因材質優良獲選臺灣闊五木之一,且為瀕臨絕種之寬尾鳳蝶唯一食草,該物種在生態、經濟、學術上皆佔有重要地位,但相對於檫木及北美檫樹兩種在原生地廣泛分布的樹種,臺灣檫樹族群僅零星分布且人工繁殖困難,為IUCN列名之易受害物種。本研究係以遺傳層面探討臺灣檫樹之物種保育,以具有高突變率的17組微衛星體分子標記及葉綠體分子標記rps16 -trnK、psbD-trnT兩片段進行研究,主要探討目標為:(1) 以分子資料檢視族群遺傳結構,以探討目前臺灣檫樹所面臨之問題。(2) 推估歷史族群變動及遷移方向。(3) 瞭解在全球氣候劇烈變遷的影響下,臺灣檫樹族群量及分布的改變。(4) 擬具臺灣檫樹的保育方針。
本研究於臺灣本島十個臺灣檫樹分布族群共採集142株個體,結果顯示臺灣檫樹分為北部及南部兩群,族群內近親交配程度高,且族群間分化程度及距離隔離效應明顯,顯示族群間基因交流程度低,且臺灣檫樹母樹林內小苗生存率低、林木生長狀況不佳,故推論臺灣檫樹族群面臨嚴重近交衰退,使後代繁殖困難,同時,對照貝氏天際線分析顯示未來族群量可能會持續下降。
以生態棲位模擬臺灣檫樹在末次盛冰期可分布至中低海拔,臺灣檫樹最原始的祖先推測為阿里山族群,經由連續性的族群範圍擴張至東部及中部,北部各族群間基因交流受侷限而在各區域產生獨特的基因單型。在面對全球環境變遷的情況下,臺灣檫樹族群在2080年可能面臨滅絕的危機。因此建議除目前就地保育方案外,應適當的促進族群間基因交流,並同時進行保種作業,以維持基因多樣性,避免該物種的消失。


目錄
摘要............................................I
Abstract.......................................II
目錄............................................IV
圖目錄..........................................VI
表目錄..........................................VII
1 前言....................................1
1.1 檫樹屬之生物地理學........................1
1.2 臺灣檫樹之介紹............................2
1.3 臺灣檫樹與小規模族群遺傳研究...............4
1.4 分子標記.................................7
1.5 研究目的.................................9
2 材料與方法...............................10
2.1 樣本採集.................................10
2.2 DNA萃取..................................12
2.3 微衛星基因片段分析.........................16
2.4 葉綠體基因片段分析.........................22
2.5 生態棲位模擬..............................26
3 結果.....................................28
3.1 微衛星基因座變異分析.......................28
3.2 葉綠體基因座變異分析.......................42
3.3 生態棲位模擬..............................52
4 討論.....................................54
4.1 臺灣檫樹之遺傳多樣性.......................54
4.2 臺灣檫樹親緣關係及族群間顯著的距離隔離效應....56
4.3 族群遺傳結構及分化.........................57
4.4 推估臺灣檫樹過去及未來之族群動態.............58
4.5 臺灣檫樹之保育建議 .........................60
5 結論......................................63
6 參考文獻..................................65


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