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研究生:楊貽婷
研究生(外文):Yi-Ting Yang
論文名稱:探討Puf6與Loc1於核醣體大單元體60S生合成途徑中功能之關聯性
論文名稱(外文):Study the Functional Connection between Puf6 and Loc1 in 60S Biogenesis
指導教授:羅凱尹
指導教授(外文):Kai-Yin Lo
口試委員:呂俊毅黃偉邦冀宏源林晉玄
口試委員(外文):Jun-Yi LeuWei-Pang HuangChi-Hung YuanChing-Hsuan Lin
口試日期:2015-07-22
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:農業化學研究所
學門:農業科學學門
學類:農業化學類
論文種類:學術論文
論文出版年:2015
畢業學年度:103
語文別:中文
論文頁數:71
中文關鍵詞:核醣體核醣體生合成Puf6Loc1Rpl43
外文關鍵詞:RibosomeRibosome biogenesisPuf6Loc1Rpl43
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在真核細胞中,Puf6與Loc1同時參與兩條重要的途徑: mRNA之不對稱運輸,以及核醣體生合成。此外,Puf6與Loc1在細胞中主要分布位置皆位於核仁。此兩個蛋白結合ASH1 mRNA,將mRNA運輸至子細胞尖端(bud tip),而mRNA之不對稱分布對於真核細胞之分化相當地重要。另外,Puf6與Loc1也被發現與60S生合成有關聯。若將細胞中PUF6或LOC1移除,pre-rRNA之剪切 (Processing),60S之出核情形,以及60S的生成量皆會受到影響。然而,目前對於Puf6與Loc1之研究大部分是針對ASH1 mRNA運輸之途徑,關於此兩個蛋白如何於60S生合成途徑執行其功能,目前仍尚未明瞭。
在此篇研究中,發現RPL43是puf6∆之High-copy suppressor,以及Puf6、Rpl43與Loc1三個蛋白質之間具有直接的結合,且於60S生合成途徑中的功能有緊密之關聯性。Loc1為Rpl43之伴護子,並和Puf6協助Rpl43結合60S。且Puf6及Loc1需要彼此才能在正確的時間點結合60S。接著,待Puf6與Loc1執行完其功能後,離開60S則需要Rpl43。


Puf6 and Loc1 have two important functional roles in the cells, asymmetric mRNA distribution and ribosome biogenesis. Puf6 and Loc1 are localized predominantly in the nucleolus. They bind ASH1 mRNA, repress its translation, and facilitate the transport to the daughter cells. Asymmetric mRNA distribution is important for cell differentiation. Besides, Puf6 and Loc1 have been shown to involve in 60S biogenesis. In puf6∆ or loc1∆ cells, the pre-rRNA processing and 60S export are impaired and 60S subunits are under-accumulated. The functional studies of Puf6 and Loc1 have been focused on ASH1 mRNA pathway, but the functional roles in 60S biogenesis are still not clear.
In this study, we identified that RPL43 is the high-copy suppressor of puf6∆. Besides, Puf6, Loc1 and Rpl43 have direct physical interaction, and these proteins are tightly connected in 60S biogenesis. Loc1 is the chaperon of Rpl43. Loc1 and Puf6 facilitate the loading of Rpl43. Furthermore, Puf6 and Loc1 depend on each other for joining 60S biogenesis pathway properly. Finally, the recruitment of Rpl43 is required for release of Puf6 and Loc1.


目錄
一、文獻回顧與探討 1
1.1 核醣體生合成 1
1.1.1 rRNA之生成與剪切 1
1.1.2 核醣體之組裝 2
1.1.3 40S之組裝 3
1.1.4 60S之組裝 4
1.1.5細胞核內外物質運輸之調控 5
1.1.6 60S出核之調控 6
1.2 ASH1 mRNA主動運輸 8
1.3與本研究相關之因子 10
二、研究動機與問題 12
三、研究材料與方法 14
3.1 質體之建構 14
3.1.1 PCR 14
3.1.2 電泳 14
3.1.3 純化DNA膠體 14
3.1.4 限制酶切割 14
3.1.5 接合 15
3.1.6 選殖菌體 15
3.2 轉型至啤酒酵母 15
3.3 螢光顯微鏡 15
3.4 生長測試 16
3.5 High-copy suppressor screen 16
3.6核醣體圖譜分析(polysome profile) 16
3.7 Sucrose cushion 17
3.8免疫沉澱 17
3.9 In vitro interaction 18
四、結果 19
4.1 Puf6和Loc1參與60S生合成之階段相似 19
4.2 Puf6與Loc1協助彼此於正確之階段執行功能 21
4.3 於loc1Δ去除PUF6基因可以部分修復loc1Δ突變株之缺失 22
4.4 PUF6之High-copy suppressor為RPL43B 23
4.5 Puf6與Loc1協助Rpl43結合60S 24
4.6 Rpl43、Puf6與Loc1三者之間有直接之結合 26
4.7 Loc1協助Rpl43維持其穩定性 27
4.8 Rpl43幫助Puf6與Loc1離開60S 28
4.9 分析Puf6的功能性區塊 30
4.10 分析Loc1的功能性區塊 32
五、結論 33
六、討論 34
6.1 Rpl43、Loc1與Puf6於60S生合成途徑中之關聯 34
6.2 Puf6與Loc1參與60S生合成之階段 35
6.3 RPL43B無法修復loc1Δ所造成之生長缺失 36
6.4 Puf6於loc1Δ之分布由核仁擴散至細胞核 37
七、參考文獻 38












表目錄
表一、本研究中使用之啤酒酵母菌株 46
表二、本研究中使用之質體 47
































圖目錄
Figure 1. Puf6和Loc1參與60S生合成之階段相似 49
Figure 2. Puf6與Loc1協助彼此於正確之階段執行功能 51
Figure 3. 去除PUF6基因可以部分修復loc1Δ突變株之缺失 53
Figure 4. puf6Δ之High-copy suppressor為RPL43B 54
Figure 5. Puf6與Loc1協助Rpl43結合60S 55
Figure 6. Rpl43、Puf6與Loc1三者之間有直接之結合 56
Figure 7. Loc1協助Rpl43維持其穩定性 57
Figure 8. Rpl43B幫助Puf6與Loc1離開60S 60
Figure 9. 分析Puf6的功能性區塊 64
Figure 10. 分析Loc1的功能性區塊 66






















附錄目錄
附錄一、 rRNA加工示意圖 68
附錄二、 ASH1 mRNA主動運輸示意圖 69
附錄三、 60S生合成之不同階段示意圖 70
附錄四、 大量表現RPL43無法修復loc1Δ之生長缺失 71


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