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研究生:黃浩瑋
研究生(外文):Hao-Wei Huang
論文名稱:旋轉碗形缸用於Oikopleura dioica尾蟲飼育之研究
論文名稱(外文):Study of The Rotating Bowl-Shaped Tank For The Appendicularian, Oikopleura dioica Rearing
指導教授:羅秀婉
指導教授(外文):Show-Wan Lou
口試委員:徐崇仁王永松
口試日期:2015-07-20
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:漁業科學研究所
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2015
畢業學年度:103
語文別:中文
論文頁數:53
中文關鍵詞:尾蟲餌料生物旋轉碗形缸水流
外文關鍵詞:Oikopleura dioicafood organismrotating bowl-shaped tankwater flow
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Oikopleura dioica種尾蟲已被視為生物研究之模式動物並在國外行之有年,而在國內研究仍稀少,其可利用領域除了海洋生物學、環境毒物學、基因體學與農學外,也曾被討論作為餌料生物之可能性。為使Oikopleura dioica飼育更接近產業化養殖,本研究從研發適用於尾蟲飼育的特殊缸體切入,目標為以新式設計碗形缸達成尾蟲飼育及簡化飼養中的日常管理操作,研究中同時使用當前尾蟲研究者採用之飼育器材飼養尾蟲,並對兩類器材之飼育成效進行比較。結果顯示新式碗形缸形成之水流除能匯集生物於飼育水體中央,最高約可有70%浮游生物個體聚集且水流狀態平緩,而現行缸體則無分內外水體各採樣點百分約為
1.7±0.36%至3.9±1.5%且底層個體數量高達10%以上,顯示旋轉碗型缸或可減少生物因接觸接觸缸壁與受水流衝擊而造成損傷之機會。在尾蟲飼育成果,新式碗形缸可飼育尾蟲至生殖成熟,實驗期間活存率趨勢與現行缸體相近,而實驗終了之生物活存率旋轉碗形缸可達49.0%較現行缸體高出13.0%,有更好的表現。經歷6天飼育飼育後水質結果,旋轉碗形缸換缸可將氨氮濃度維持於0.25 ppm以下,而現行缸體約為0.75 ppm。


口試委員會審定書 i
謝辭 ii
摘要 iii
Abstract iv
目錄 v
第一章 前言 1
1.1尾蟲概述 1
1.2尾蟲飼育研究回顧 2
1.3尾蟲飼育器材回顧 2
1.4新式器材設計方向 3
1.5研究方法與目標 3
第二章 材料與方法 5
2.1實驗架構 5
2.2實驗器材 5
2.2.1旋轉碗形缸 5
2.2.2緩速擾流罐 6
2.3實驗動物 6
2.3.1浮游動物分佈與水流觀察實驗 6
2.3.2 野生尾蟲實驗 6
2.3.3.人工尾蟲實驗 7
2.4實驗設計 7
2.4.1器材運作中浮游動物分佈與水流觀察 7
2.4.2尾蟲飼育觀察 8
2.4.3每日缸體清潔 9
2.5資料分析 9
2.5.1水流狀態觀察 9
2.5.2尾蟲飼育 9
第三章 結果 11
3.1旋轉碗形缸水流觀察 11
3.1.1水流狀態觀察 11
3.1.2豐年蝦無節幼蟲採樣 11
3.2緩速擾流罐豐年蝦無節幼蟲採樣 13
3.3尾蟲飼育 13
3.3.1人工尾蟲飼育 13
3.3.2野生尾蟲飼育 13
第四章 討論 15
4.1器材與實驗設計 15
4.2豐年蝦無節幼蟲採集 16
4.3水流狀態 16
4.4尾蟲飼養 17
4.5旋轉碗形缸之意義與展望 18
第五章 結論 19
參考文獻 20
附錄 27


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