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研究生:陳俊廷
研究生(外文):Chun-Ting Chen
論文名稱:動物醫院中微生物分佈與環境因子之關係
論文名稱(外文):The Relationship between Microbial Pathogens and Environmental Factors in Veterinary Hospitals
指導教授:林辰栖
口試委員:廖泰慶周崇熙邱亦涵
口試日期:2015-07-02
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:獸醫學研究所
學門:獸醫學門
學類:獸醫學類
論文種類:學術論文
論文出版年:2015
畢業學年度:103
語文別:英文
論文頁數:127
中文關鍵詞:職業危害生物氣膠真菌細菌人畜共通傳染病
外文關鍵詞:occupational hazardsbio-aerosolfungusbacteriumzoonotic disease
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獸醫職業與相關從業人員暴露於各式各樣職業危害的風險,例如:物理性危害、化學性危害、生物性危害。在臨床與流行病學的研究調查報告指出,獸醫在許多職業疾病方面,處於不斷增加的風險,包含部位特定癌症、胎兒流產、急性相關農藥毒性、人畜共通傳染病、職業皮膚病及呼吸道疾病。在台灣,目前只有在民國88年的一項調查報告顯示,本地獸醫職業所面臨的職業危害,大多數來自於動物的抓、咬或踢。大部分的生物性危害因子會以氣膠形式分佈在職業工作場所中。然而,目前沒有進一步的建議或努力,已改善這樣的狀況。本文研究旨在探討獸醫及相關工作人員在他們的工作場所,透過空氣中採樣的方式,進行微生物病原調查。使用衝擊式(impactor)標準生物氣膠採樣器進行生物氣膠採樣,此採樣器上有400孔,孔徑為0.25 mm,採樣流量是28.3 L/min,且採樣高度則為距離地面1-1.5 m高,同時記錄環境因子條件,包含溫度與濕度。採樣結束後,樣品進行培養和分析。細菌利用抽取DNA進行16S rDNA 序列比對鑑定,真菌則是抽取DNA進行18S rDNA 序列比對鑑定。因此,在目前所獲得結果的研究中,最常出現的真菌菌屬是Coriolopsis spp. 和 Microporus spp.,及最常出現的細菌菌屬是Micrococcus spp. 和 Staphylococcus spp. 。因此,我們希望這將有助於我們了解在獸醫臨床上可能產生的危害,已可以在不久的將來得到進一步修正。

Contents
誌謝 I
中文摘要 II
Abstract III
Contents V
Chapter 1 Introduction 1
Chapter 2 Literature Review 3
2.1. Occupational hazard factors of veterinary medicine 3
2.2. Physical hazards 4
2.3. Chemical hazards 6
2.3.1. Anaesthetic gases 6
2.3.2. Pesticides 7
2.3.3. Formalin 7
2.3.4. Abuse of drug and suicide 8
2.4. Biological hazards 8
2.4.1. Common fungus bio-aerosol in indoor environments 9
2.4.2. Common bacteria bio-aerosol in indoor environments 10
2.4.3. Allergens 10
2.4.4. Zoonotic diseases 11
2.5. Indoor air quality (IAQ) 12
Chapter 3. Materials and methods 14
3.1. Sampling areas 14
3.2. Preparation of media plates 16
3.2.1. Trypticas soy agar (TSA) 16
3.2.2 Malt extract agar (MEA) 16
3.3. Sampling collection 16
3.4. Identification of microbial organisms 18
3.4.1. DNA extraction for 16S ribosomal DNA of bacterium 18
3.4.2. DNA extraction for 18S ribosomal DNA of fungus 18
3.5. Sequence analysis and alignment 19
3.6. Statistical analysis 20
Chapter 4. Results 22
4.1. The ranged of environmental factors in different teaching hospitals and clinics 22
4.1.1. Teaching hospitals and clinics 22
4.1.2. Teaching hospital A 22
4.1.3. Teaching hospital B 23
4.1.4. Teaching hospital C 24
4.1.5. Clinic D 25
4.1.6. Clinic E 25
4.1.7. Clinic F 26
4.1.8. Clinic G 27
4.1.9. Clinic H 28
4.2. The distribution differences of fungus in different teaching hospitals and clinics 29
4.2.1. Teaching hospitals and clinics 29
4.2.2. Teaching hospital A 29
4.2.3. Teaching hospital B 30
4.2.4. Teaching hospital C 31
4.2.5. Clinic D 32
4.2.6. Clinic E 32
4.2.7. Clinic F 33
4.2.8. Clinic G 34
4.2.9. Clinic H 34
4.2.10. Statistic analysis 35
4.3. The distribution difference of bacteria in different teaching hospitals and clinics 36
4.3.1. Teaching hospitals and clinics 36
4.3.2. Teaching hospital A 36
4.3.3. Teaching hospital B 37
4.3.4. Teaching hospital C 38
4.3.5. Clinic D 38
4.3.6. Clinic E 39
4.3.7. Clinic F 40
4.3.8. Clinic G 40
4.3.9. Clinic H 41
4.3.10. Statistic analysis 42
4.3.11. Statistic difference between environment factor and microbial 42
4.3.11.1. Statistic difference for teaching hospital A 44
4.3.11.2. Statistic difference for teaching hospital B 44
4.3.11.3. Statistic difference for teaching hospital C 45
4.3.11.4. Statistic difference for clinic D 46
4.3.11.5. Statistic difference for clinic E 46
4.3.11.6. Statistic difference for clinic F 47
4.3.11.7. Statistic difference for clinic G 48
4.3.11.8. Statistic difference for clinic H 49
Chapter 5. Discussion 51
Reference 61


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