臺灣博碩士論文加值系統

噴霧熱解法被成功的用於合成不同SiO2-CaO-P2O5成分之介孔生物活性玻璃(Mesoporous bioactive glasses, MBGs)，於本研究之重點為將分別58S、68S以及76S等三種不同成分之MBG對其生物活性與降解性進行更深入得探討。人體模擬體液(Simulated body fluid, SBF) 被做為MBG之降解性以及生物活性檢測之介質進行為期30天的體外生物檢測，期間每天對於MBG之塊材進行量測其重量，並記錄重量損失，並每天替換SBF同時測量其pH值用已推測MBG於浸泡入SBF之中可能產生之反應。最後分別使用X光繞射儀進行其相分析鑑定、傅立業轉換紅外線光譜儀得知其化學鍵結之改變、及其表面形貌以及成份分析運用掃描式電子顯微鏡及能量色散光譜之技術得到結果。在本研究之中探討了MBG之成份對於降解性以及生物活性之影響，並於研究結果顯示58S MBG有最快的降解速率其次為68S最後為76S，其中68S以及76S MBG在浸泡入SBF之後一天即形成磷灰石層，然而58S需要三天的時間，正說明MBG的降解行為與磷灰石層的形成有非常重要的關聯，以此對於MBG降解性與磷灰石層形成之機制也做了更進一步的探討。

Spray pyrolysis has been successfully used to synthesize mesoporous bioactive glasses (MBGs) with different compositions of SiO2-CaO-P2O5. In this study, the degradability and bioactivity of 58S, 68S and 76S MBG samples were studied. Simulated body fluid (SBF) solution was used as a medium to conduct the degradation test and also bioactivity for 30 days. The weight loss of MBG bulks were measured every day during the in-vitro degradation test. The pH value changes in SBF solution was also monitored every day in order to know the reaction that may occur in SBF solution. Some characterization methods, including x-ray diffraction analysis (XRD), fourier transform infrared (FTIR), scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) have been used to know the phase changes, chemical bonding, surface morphology and compositional analysis respectively. In this research, the effect of composition on the degradation behavior and bioactivity were discussed. Since the degradation behavior of MBG was correlated to the apatite conversion. Furthermore, the mechanism of biodegradability on MBG to apatite conversion was also investigated. 58S MBG had the high degradation rate followed by 68S and 76S respectively. 68S and 76S MBG had ability to form apatite phase with 1 day of immersion while 58S MBG required 3 days of immersion.

摘要 I
ABSTRACT II
ACKNOWLEDGEMENTS III
CONTENTS IV
LISTS OF TABLES VII
LISTS OF FIGURES VIII
CHAPTER 1 INTRODUCTION 1
CHAPTER 2 LITERATURE REVIEW 4
2.1 Bone and scaffolding 4
2.1.1 Basic of bones 4
2.1.2 Scaffolding 6
2.2 Bioceramics 9
2.3 Bioactive materials 12
2.3.1 Introduction 12
2.3.2 Characteristics 12
2.3.3 Types 14
2.4 Bioactive glass 15
2.4.1 Compositions 16
2.4.2 Synthesis methods 18
2.4.3 Bonding mechanism of bioactive glass 20
2.5 Mesoporous bioactive glass 23
2.5.1 Introduction 23
2.5.2 Synthesis methods 25
2.6 Degradation of bioactive glass through apatite conversion and the effect of its compositions 26
2.7 Spray pyrolysis 28
2.7.1 Equipment 29
2.7.2 Particle formation mechanism 31
CHAPTER 3 MATERIAL AND EXPERIMENTAL PROCEDURE 32
3.1 Chemicals 32
3.2 Experimental equipment 33
3.3 Material preparation 34
3.3.1 Spray pyrolysis procedures 34
3.3.2 Die pressing and sintering 37
3.4 Materials characterization 37
3.4.1 X-ray diffraction 38
3.4.2 Fourier transform infrared spectroscopy 38
3.4.3 Scanning electron microscope 38
3.4.4 Transmission electron microscope 39
3.4.5 Thermogravimetric analysis 39
3.5 In-vitro degradation test 40
3.5.1 Weight loss test 40
3.5.2 pH change 43
3.5.3 Bioactivity 43
CHAPTER 4 RESULTS AND DISCUSSION 44
4.1 Results 44
4.1.1 Initial characterizations of MBG powders 44
4.1.1.1 Morphological and compositional analysis 44
4.1.1.1 Transmission electron microscope 47
4.1.1.2 Phase analysis 50
4.1.1.3 Thermal analysis 55
4.1.1.4 Chemical bonding analysis 56
4.1.2 In-vitro degradation test 57
4.1.2.1 Accumulated weigh loss 58
4.1.2.2 pH changes in SBF solution 60
4.1.3 Phase and chemical bonding identification after in-vitro test 62
4.1.3.1 Phase analysis 62
4.1.3.2 Chemical bonding analysis 66
4.1.4 Morphological and compositional analysis after in-vitro test 70
4.1.4.1 Surface morphology 70
4.1.4.2 Change of Si, Ca, P elements on surface of MBG 74
4.2 Discussions 78
4.2.1 Initial characterization of MBG powder 78
4.2.1.1 Morphological and compositional analysis 78
4.2.1.2 Crystallization of as-received MBG powders 80
4.2.1.3 Remained carbon during spray pyrolysis process 83
4.2.2 In-vitro degradation test and bioactivity 83
4.2.2.1 Degradability of MBGs 84
4.2.2.2 Bioactivity of MBGs 89
4.2.3 Mechanism of apatite growth on MBG surface 93
CHAPTER 5 CONCLUSIONS 100
CHAPTER 6 FUTURE WORKS 101
REFERENCES 102

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