鉛蓄積於人體會誘發氧化性壓力，造成嚴重的發炎反應，導致許多組織與器官受損，進而影響生物體正常的生長發育，嚴重甚至可能導致死亡。鉛中毒的臨床治療，目前主要使用熬合劑或洗胃來清除循環與消化系統中殘餘的鉛，但這些治療方式無法有效的降低蓄積於組織或器官中的鉛。過去文獻指出遠紅外線(非一般的三溫暖或運動)，可促進鉛經由汗液排出，遠紅外線因此被認為可能有助於加速鉛的排除，或許在治療及預防鉛中毒方面可以發揮作用。本篇論文利用特定波長(9~14μm)之遠紅外線照射皮膚角質細胞( HaCaT )，在未通電且沒有明顯溫度變化的情況下，證實遠紅外線確實可以加速傷口癒合。細胞實驗中我們發現，鉛會誘導細胞內的氧化性壓力(R.O.S.)上升導致腎臟細胞死亡，而遠紅外線處理組，細胞內鉛含量與氧化性壓力相對較低。在動物實驗中，老鼠持續飲用鉛水(3g/500ml)七週，之後將之分為兩組，一組不做任何處理(鉛單獨處理組)，另一組則定時給予遠紅外線照射，量測老鼠血液及尿液中鉛濃度後發現，鉛單獨處理組血中鉛濃度(71μg/ml)明顯高於遠紅外線治療組(30μg/ml)。此外，鉛所造成的老鼠體重減輕與腎絲球病變，在遠紅外線治療組明顯比鉛單獨處理組少。我們的研究結果顯示遠紅外線或許可以透過加速鉛代謝，降低鉛的蓄積與所誘導的氧化性壓力，降低鉛對腎臟造成的傷害。

Lead, once accumulated in the body, can cause oxidative stress, leading to severe inflammation, organ injury, inhibition of growth development, and even death. Presently, clinical treatments, including both gastrointestinal decontamination and chelation therapy, cannot effectively reduce lead accumulation in tissues or organs. As previous studies showed that far-infrared (FIR) exposure removed a significant amount of lead from the body through sweat excretion, therefore, we investigated whether FIR had a beneficial effect on the treatment of lead toxicity. To examine the effect, pure FIR ceramic semiconductor (c-FIR), ranged from 9 to 14 μm, was adopted in this study. Previous studies showed that FIR promoted cell migration and stimulated cell proliferation, our studies using wound-healing assays in keratinocytes (HaCaT) show that c-FIR produces similar results without thermal effects. Using renal cells as in vitro model, our results show that, in comparison to controls, c-FIR markedly reduced ROS production and its consequent cell death. In vivo studies were conducted with two groups of rats fed with drinking water containing lead for 7 weeks, with or without FIR-cotreatment, and the concentration of Pb in blood and urine were determined. Blood concentrations of Pb in rats that were exposed to Pb with co-application of FIR were significantly lower (30μg/ml) than those without FIR exposure (71μg/ml). Moreover, the weight loss and glomerulosclerosis caused by Pb consumption were significantly reduced in the FIR-treated group. We also find that FIR can accelerate the metabolism of lead in rats. Overall, our data supports the notion that FIR may provide a novel therapeutic avenue for the treatment of lead toxicity.

目錄............ 1
縮寫表.......... 5
中文摘要........ 7
英文摘要........ 8
第壹章、 緒論.... 9
第一節 過去的研究....... 9
第二節 研究目標.......... 14
第貳章、 實驗材料與方法... 15
第一節 實驗材料........ 15
(一) 一般藥品試劑........ 15
(二) 遠紅外線陶瓷半導體與照射器.... 16
(三) 一般儀器............ 16
(四) 一般溶液之配置...... 17
(五) 細胞株............. 17
(六) 試驗動物........... 17
第二節 實驗方法........ 18
(一) 遠紅外線陶瓷半導體發射光譜分析........ 18
(二) 溫度測量............ 18
(三) 細胞解凍.... ........18
(四) 細胞培養............ 19
(五) 細胞冷凍............ 19
(六) 細胞計數............ 20
(七) 傷口癒合實驗( Wound healing assay).. 20
(八) 細胞存活率偵測分析( MTT assay)....... 20
(九) 偵測鉛濃度.......................... 21
(十) 測量自由基( Free radical)含量....... 21
(十一) 動物實驗.......................... 21
(十二) 組織切片染色( H&E stain).......... 22
(十三) 統計分析.. ........................22
第參章、 實驗結果........................ 23
第一節 遠紅外線陶瓷半導體發射光譜表現....... 23
第二節 遠紅外線陶瓷半導體對細胞培養環境溫度之影響 ........................................23
第三節 遠紅外線促進皮膚角質細胞傷口癒合及增生....... 24
第四節 鉛蓄積造成腎小管細胞( NRK52E)之毒性. 24
第五節 遠紅外線降低腎小管細胞內鉛蓄積的濃度. 26
第六節 遠紅外線降低鉛誘導腎小管細胞產生之氧化性壓
力...................................... 26
第七節 遠紅外線改善鉛毒性造成的生長抑制..... 27
第八節 遠紅外線降低血中鉛濃度並提升腎臟代謝能力 ........................................27
第九節 遠紅外線加速鉛的排出....... 28
第十節 遠紅外線保護鉛誘導之腎臟損傷........ 29
第肆章、 討論............................ 30
第伍章、 結論............................ 32
參考文獻................................ 33
實驗結果圖表..... ........................37
圖一. 遠紅外線陶瓷半導體發射光譜........... 37
圖二. 遠紅外線對細胞培養環境溫度的影響...... 38
圖三. 遠紅外線促進皮膚角質細胞傷口癒合及增生 39
圖四. 鉛蓄積造成腎小管細胞( NRK52E)之毒性.. 40
圖五. 遠紅外線降低腎小管細胞( NRK52E)內鉛蓄積的
濃度.................................... 42
圖六. 遠紅外線降低鉛誘導腎小管細胞產生之氧化性壓
力...................................... 43
圖七. 遠紅外線改善鉛毒性造成的生長抑制...... 44
圖八. 遠紅外線降低血中鉛濃度並提升腎臟代謝能力...... 45
圖九. 遠紅外線加速鉛的排出................ 46
圖十. 遠紅外線保護鉛誘導之腎臟損傷......... 47

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