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研究生:林淑靜
研究生(外文):LIN, SHU-CHING
論文名稱:以不同尿素酶檢測試劑,進行胃幽門桿菌靈敏度比較之研究
論文名稱(外文):Comparison Studies for the Effect of Different Urease Detection Reagents on the Sensitivity of the Gastric Helicobacter pylori
指導教授:張淑微張淑微引用關係
指導教授(外文):CHANG, SHU-WEI
口試委員:張世良林娉婷
口試委員(外文):CHANG,SHIH-LIANGLIN PING-TING
口試日期:2016-07-28
學位類別:碩士
校院名稱:大葉大學
系所名稱:生物科技碩士在職學位學程
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2016
畢業學年度:104
語文別:中文
論文頁數:75
中文關鍵詞:胃幽門桿菌胃潰瘍胃癌尿素酶靈敏度
外文關鍵詞:Helicobacter pylorigastric ulcerstomach cancerUreaseSensitivity
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世界上大約有一半的人口遭受胃幽門桿菌(Helicobacter pylori)的感染,其中亦包含約50%的台灣成年人,由於胃幽門桿菌感染者較易得到胃癌癌前病變,因此世界衛生組織也已經將胃幽門桿菌列為胃癌發生的一級致病因子。而根除胃幽門桿菌感染可以解決胃潰瘍、十二指腸潰瘍,並且可以防止胃癌的發生。一個簡單、快速和經濟的檢測試劑,能夠準確地識別胃幽門桿菌感染,且可加快醫生治療的決定,顯得格外重要。有鑑於利用胃幽門桿菌會產生尿素酶的特性所發展的胃幽門桿菌尿素酶測試組,為患者感染胃幽門桿菌的診斷工具,具有重要的價值,本研究將不同菌落數的胃幽門桿菌以市售不同廠牌胃幽門桿菌尿素酶測試組(ASAN和IF)用不同濃度的菌落數及不同溫度進行比較,用以探討二種產品的靈敏度。研究結果顯示市售的IF胃幽門桿菌尿素酶測試組只需要5×105 CFU/mL的菌落數,室溫16小時便可偵測是否感染胃幽門桿菌,37℃培養箱的環境下,5×105 CFU/mL的菌落數,只要1小時,就可偵測是否感染胃幽門桿菌,表示市售IF胃幽門桿菌尿素酶測試組是具有相當高的靈敏度。
Infection with Helicobacter pylori (H. pylori) is a problem affecting around half of the world’s population, among which are approximately 50% of the adult population in Taiwan. Due to people being infected with H. pylori suffering a higher risk to develop gastric precancerous lesions, the WTO has classified H. pylori as a group 1 carcinogen in stomach cancer.
Eradicating H. pylori infections can help to treat gastric and duodenal ulcers and can prevent gastric cancer. Hence, it is of crucial importance to have easy, fast and economical testing systems that detect H. pylori infections accurately and help to accelerate the therapy decision making of the physicians.
Based on the bacteria’s innate capability to produce Urease, Urease Activity Tests (CLO-Tests) serve as a highly important instrument to diagnose H. pylori infections in patients. This research uses commercial CLO-Tests from two different manufacturers (ASAN and IF) and compares their sensitivity levels at varying bacterial colony counts and temperatures.
The results of this research show that the commercial CLO-tests from IF (IF Helicobacter Test) at a colony count of 5×105 CFU/mL needs 16 hours at room temperature and only one hour at an incubation temperature of 37°C to detect infections with H. pylori, thus illustrating the high sensitivity of the IF Helicobacter Test.

目錄
封面內頁
簽名頁
中文摘要 iii
ABSTRACT iv
誌謝 v
目錄 vi
圖目錄 viii
表目錄 x

1. 前言 1
2. 文獻回顧 2
2.1 胃潰瘍(Stomach ulcer) 4
2.2胃幽門螺旋桿菌 5
2.2.1發現與命名 5
2.2.2胃幽門桿菌感染的途徑及時期 7
2.2.3檢測方法 8
3.材料與方法 18
3.1 材料與試藥 ...18
3.1.1 菌株 18
3.1.2 試藥 18
3.1.3 耗材 19
3.1.4儀器設備 20
3.2實驗與方法 21
3.2.1實驗流程 21
3.2.2 Helicobacter pylori之培養 22
3.2.3 Helicobacter pylori保存 24
3.2.4 Helicobacter pylori菌株確認 24
3.2.5菌落數測定 27
3.2.6  37℃培養箱溫度測定 28
4. 結果與討論 30
4.1 不同時間偵測的影響 31
4.2 不同菌落濃度的影響 33
4.3 不同溫度偵測的影響 46
5.結論 52
參考文獻 53
附錄 59


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