臺灣博碩士論文加值系統

隨著科技的不斷發達與進步，電磁場早已無所不在於我們的生活當中。而近幾年許多研究團隊投入對電磁場與生物相互關係的研究裡，無論是從正常的人類細胞甚至到癌細胞都對電磁場有不同程度上的反應,現在有越來越多的研究結果顯示癌症細胞對於極低頻電磁場具有高度的敏感性，在電磁場的刺激下癌症細胞會有抑制現象的發生。現今相關的理論基礎多建立在鈣離子共振後引起的鈣離子傳輸改變來解釋其中可能的機制，因此針對鈣離子共振頻率來進行一連串的探討。基於以上的理由，我們嘗試利用舒曼共振頻率7.83 Hz結合電磁場來探討其對黑色素腫瘤細胞B16F10的影響性，並進一步利用掃頻的方式來測試是否會有不同的結果。
我們的研究結果顯示，B16F10癌症細胞在舒曼共振頻率7.83 Hz電磁場刺激之下有明顯的抑制現象，除此之外，癌細胞的存活率隨著掃頻區間不同也有所變化，而我們推論此結果可能與鈣離子共振現象有關，進一步影響到癌細胞正常的生長。

Along with the advancement of technology, electromagnetic field (EMF) is omnipresent in our daily lives nowadays. Recently, many research teams invest effort at the influence between EMF and biological. From normal cells to cancer cells, they have a difference response under EMF stimulation. There are more and more researches have indicated that cancer cells possess a high sensitivity to the extreme-low frequency EMF, most of them display an obviously inhibitory effects. Many related theories are established in the resonance effect of calcium ions, leading an abnormal ion transport to change cell functions, further investigate possible resonance frequency calcium ion. Base on above reasons, we attempt to use 7.83 Hz which is called Schumann resonance frequency, combining with low magnetic field intensity to influence B16F10 cancer cells.
Our results showed an obviously inhibitory effects under 7.83 Hz and presented a tendency with different frequency sweep intervals. Then we inference that the results may be associated with calcium ion resonance effect.

CONTENTS
中文摘要……………………………………………………….……………………I
ABSTRATE…………………………………………………………………………II
ACKNOELEDGE…………………………………………………………………III
CONTENTS………………………………………………………………………V
LIST OF TABLE………………………………………………………………..VII
LIST OF FIGURE………………………………………………………………VIII
CHAPTER 1 INTRODUCTION…………………………………………….1
1-1 Background and Motivation…….………………………………………...…1
1-1-1Background……………………………………………………………...1
1-1-2 Motivation………………………………………………………………3
1-2 EMF Theoretical Mechanisms on cells…………………………...…………7
CHAPTER 2 MATERIAL AND METHOD…………………………13
2-1 Cell line……………………………………………………………………..13
2-2 EMF device design…………………………………………………………14
2-2-1 Signal types…………………………………………………………….15
2-2-2 Magnetic field………………………………………………………….18
2-3 Cell analysis method………………………………………………………..21
2-3-1 MTT assay………………………………………………………..……21
2-3-2 Optical density value (O.D value)……………………………………..21
2-3-3 Statistical analysis……………………………………………………...22
CHAPTER 3 EXPERIMENT SETUP………………………………..23
3-1 Experimental setups…………………………………………………………23
3-2 Exciting condition......................25
3-2-1 Frequency performs……………………………………………………25
3-2-2 Magnetic field magnitude……………………………………………...25
3-2-3 Durations………………………………………………………………25
CHAPTER 4 EXPERIMENT RESULTS AND DISSCUSION……..27
4-1 Experiment results………………………………………………………….27
4-1-1 Experiment results of 7.83 Hz……….………………………………27
4-1-2 Experiment results of 7.83±0.3 Hz…………………………………...30
4-1-3 Experiment results of 7.83±1 Hz……………………………………..33
4-1-4 Experiment results of 7.83±2 Hz…………………………………..…36
4-1-5 Experiment results of 7.83±0.5 Hz…………………………………...37
4-1-6 Experiment results of 7.83±0.1 Hz………………………………...…39
4-2 Discussions of experiment results…………………………………………40
CHAPTER 5 CONCLUSIONS………………………………….…...47
REFERENCES……………………………………………………………………….48

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