臺灣博碩士論文加值系統

　　本研究探討以吳郭魚水解蛋白及水解大豆蛋白，添加及取代在飼料當中對點帶石斑成長與體組成之影響。
　　第一個實驗當中，添加吳郭魚水解蛋白0、1、2、3、4、5%於飼料中，配製成6組等蛋白(46%)及等油脂(11%)之實驗飼料，魚粉、蝦粉及烏賊粉作為蛋白來源，每組實驗做三重複；實驗魚體初重1.26±0.01克；每日投餵三餐，投餵量為魚體體重的6%；實驗週期為8週。實驗結果顯示增重率在添加3％水解蛋魚白的處理組(2690.89%)顯著高於添加5%水解魚蛋白的處理組(1995.40%)，而其他各組間沒有顯著差異；魚體肌肉組織粗蛋白、粗油脂、水分及灰分隨著飼料中水解魚蛋白添加量上升而有下降的趨勢。因此本實驗的結論為飼料中添加3%的水解魚蛋白對點帶石斑有最好的成長表現。
　　第二個實驗當中，使用水解大豆蛋白取代飼料中0、5、10、15、20%的魚粉蛋白含量，配置成5組等蛋白(46%)及等油脂(13%)之實驗飼料，每組實驗做三重複；每缸7隻魚，初重為2.86±0.01克，實驗系統為循環水系統，每日投餵三餐，實驗為期6週。在成長實驗結束後，取代20SPH在體增重和每日特定成長率顯著低於其他處理組，而在飼料轉換率顯著高於其他處理組。魚體組織在粗蛋白、粗油脂、水灰份均無顯著差異。本實驗結論為水解大豆蛋白能作為石斑魚一良好蛋白質來源並能取代點帶石斑魚飼料中魚粉蛋白含量15%。

Two experiments were carried out to evaluate the effects of dietary tilapia protein hydrolysate (TH) and soy protein hydrolysate (SPH) on the growth performance of orange-spotted grouper Epinephelus coioides.
In the first experiment, six isonitrogenous (46%) and isolipidic (11%) diets were formulated to contain 0, 1, 2, 3, 4 and 5% tilapia protein hydrolysate. Fish meal, shrimp meal and squid meal were served as the protein sources. Triplicate groups of orange-spotted grouper (initial body weight, 1.26 ± 0.01 g) were fed six experimental diets (approximate 6% body weight) three times daily for 8 weeks. The weight gain percentages of fish fed treatment diets (1, 2, 3, 4 and 5% TH) were not significantly different from those of fish fed control diet (0% TH). However, the weight gain percentage of fish fed diet containing 3% TH was significantly higher (2690.89%) than that of fish fed diet containing 5% TH (1995.40%). The crude protein, crude lipid, moisture and ash of fish muscle tended to decrease with increasing dietary TH. Based on the experimental results, Epinephelus coioides fed diet supplemented with 3% tilapia protein hydrolysate (TH) had the best growth performance.
In the second experiment, five isonitrogenous and isolipidic diets (crude protein 46%, crude lipid 13%) were formulated to contain 0, 5, 10, 15 and 20% SPH to replace fish meal protein. Each diet was randomly assigned to triplicate tanks with 7 fish (initial weight 2.86±0.01 g) per tank in a circulating water system. Fish were fed three times daily for 6 weeks. At the end of the feeding trial, fish fed diet containing 20% SPH had the significantly lower weight gain percentage and specific growth rate than those fed other treatments. Feed conversion ratio of grouper fed 20SPH diet was significantly higher than that of grouper fed the other diets. Muscle proximate compositions of grouper showed no significantly different among dietary treatments. The soy protein hydrolysate appeared to be a good protein source for Epinephelus coioides and up to 15% SPH may be a preferable level for grouper diet without negative effects.

CHINESE ABSTRACT I
ABSTRACT II
TABLE OF CONTENTS III
LIST OF TABLES V
1. INTRODUCTION 1
2. LITERATURE REVIEW 4
2.1. Orange-spotted grouper (Epinephelus coioides) 4
2.1.1. Biology 4
2.1.2. Protein and lipid requirements 4
2.2. Current status of aquaculture and fish meal 6
2.3. Alternative protein sources for fish feeds 8
2.3.1. Fish protein hydrolysate 9
2.3.2. Soy protein hydrolysate 12
2.4. Studies on the use of protein hydrolysate in grouper diets 14
3. MATERIALS AND METHODS 15
3.1. Experimental Diets 15
3.2. Feeding Trial 16
3.3. Proximate Analysis 17
3.3.1. Moisture 17
3.3.2. Ash 18
3.3.3. Crude protein 18
3.3.4. Crude lipid 19
3.3.5. Crude fiber 19
3.3.6. Gross energy 20
3.4. Data Calculation and Statistical Analysis 20
4. RESULTS 21
4.1. Experiment I 21
4.2. Experiment II 21
5. DISCUSSION 23
5.1. Experiment I 23
5.2. Experiment II 25
6. CONCLUSION 27
REFERENCES 28

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