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研究生:龍秉廷
研究生(外文):Long, Bing-Ting
論文名稱:乳油木果萃取物對高脂飲食下半月軟骨及十字韌帶損傷誘導骨關節炎大鼠之影響
論文名稱(外文):Effects of Vitellaria paradoxa Nut Extract on Osteoarthritis of High-Fat Diet Fed Rats after Meniscal/Ligamentous Injury
指導教授:龔瑞林龔瑞林引用關係
指導教授(外文):Kong, Zwe-Ling
口試委員:鄧文炳黃效民張基隆吳亮宜廖若川
口試委員(外文):Deng, Win-PingHwang, Shiaw-MinChang, Kee-LungWu, Liang-YiLiu, Yeuk-Chuen
口試日期:2016-06-24
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:食品科學系
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2016
畢業學年度:104
語文別:中文
論文頁數:47
中文關鍵詞:骨關節炎乳油木果肥胖
外文關鍵詞:OsteoarthritisVitellaria paradoxa nutObesity
相關次數:
  • 被引用被引用:2
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  • 下載下載:15
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骨關節炎 (Osteoarthritis, OA) 是成人中最常見的關節炎疾病,通常發生在 45 歲後,危險因素如年齡,性別,肥胖,遺傳和飲食,肥胖在關節層面的因素,包括損傷和關節的異常載荷等。其臨床症狀為關節疼痛、滑液囊發炎腫脹和軟骨崩解。乳油木果萃取物已被證實具有抗氧化以及抗發炎的活性。故本研究將以乳油木果萃取物 (Vitellaria paradoxa Nut Extract, VP) 探討高脂飲食 (High fat diet, HFD) 誘發肥胖後,並給予半月軟骨及十字韌帶損傷造成骨關節炎之改善功效。動物實驗以 Wistar 雄性大鼠,給予五週 45 % 高脂飲食誘導肥胖後,進行手術,使其半月軟骨及前十字韌帶損傷,實驗分組:控制組、骨關節炎組、肥胖假手術組、肥胖手術管餵 VP 組 (111.6, 223.2, 446.4 mg/kg),持續八週。我們觀察到,餵食 VP 可以減少膝關節腫脹以及減少關節疼痛。另外, VP 可降低 TG 和增加 HDL-C 在高劑量 VP 組。其次,這項研究發現抗氧化酶酵素以及脂質過氧化反應和發炎細胞因子,包括 NO 、 IL-6 和 TNF-α 在高劑量 VP 組均降低。我們的研究表明餵食乳油木果萃取物將改善肥胖引起的骨關節炎。
Osteoarthritis is an arthritis disease that usually happened in adulthood after age of 45. Risk factors for osteoarthritis include age, sex, obesity, heredity and diet. Obesity caused osteoarthritis is attributed to joint loading, cartilage disintegration, bone loss, and inflammation as well. Treatments for this disease include moderate exercise, oral medication, intra-articular injection and artificial joint displacement surgery. Vitellaria paradoxa nut extract have been used for anti-oxidative, anti-inflammatory function. The aim of study is to investigate the effect of Vitellaria paradoxa nut extract (VP) for meniscal/ligamentous injury and high fat diet double induced-osteoarthritis. Male Wistar rats were fed 45% high-fat diet for 4 weeks to induce obesity before transecting the anterior cruciate ligament and excising a segment of the medial meniscus of the knee to initiate osteoarthritis. Rats were treated with three doses of Vitellaria paradoxa nut extract (111.6, 223.2, 446.4 mg/kg per day, p.o. daily) with 8 weeks high fat diet in parallel. We observed that the swelling in the osteoarthritis knee was the alleviated and the hind paw weight distribution was rectified after feeding Vitellaria paradoxa nut extract. The results showed that VP decrease TG and increase HDL-C level in the high dose VP group. We also observed that high dose VP may increase antioxidant enzyme, lipid peroxidation and decrease inflammation cytokines, including NO, IL-6 and TNF-α. Our study demonstrates that the feeding of Vitellaria paradoxa nut extract shall improve meniscal/ligamentous injury of obesity-induced osteoarthritis.
摘要 I
Abstract II
目錄 III
圖目錄 V
表目錄 VI
附錄目錄 VII
名詞縮寫表 VIII
壹、緒論 1
貳、文獻回顧 2
一、骨關節炎 2
二、肥胖 3
三、骨關節炎與肥胖 3
四、乳油木果 (Vitellaria paradoxa) 5
4.1 乳油木果之介紹 5
4.2 乳油木果之成分 5
4.3 乳油木果之生理活性 5
參、實驗流程 7
一、動物實驗 7
肆、實驗材料 8
一、材料與樣品 8
1.1 乳油木果樣品 8
1.2 實驗動物 8
1.3 動物飼料 8
1.4 藥品 9
1.5 儀器設備 10
伍、實驗方法 11
一、動物試驗 11
1.1 骨關節炎大鼠之誘導與分組 11
1.2 退化性關節炎之動物模式 11
1.3 實驗動物飼養 11
1.4 骨關節炎相關分析 12
1.5 血漿功能性指標評估 12
陸、統計分析 17
柒、結果 18
捌、討論 20
玖、結論 22
壹拾、參考文獻 23
壹拾壹、圖表 29
壹拾貳、附錄 43

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