臺灣博碩士論文加值系統

本研究開發一快速方法合成氧化銅/氧化亞銅/聚吡咯複合材料修飾紙電極，具有類似葡萄糖氧化酶活性的氧化銅/氧化亞銅/聚吡咯粒徑大小194 ± 6奈米，在鹼性電解液中(0.5 M 氫氧化鈉與0.2 M氯化鈉)，偵測葡萄糖具有較低的偵測電位( -0.075 伏特對於銀/氯化銀參考電極)，最低偵測極限為0.34 mM。利用掃描式電子顯微鏡、穿透式電子顯微鏡、表面增強拉曼散射光譜以及光電子能譜儀確定氧化銅/氧化亞銅/聚吡咯複合材料修飾紙電極表面的型態及組成，並將氧化銅/氧化亞銅/聚吡咯複合材料修飾紙電極於葡萄糖檢測具有低檢測電位(大約 -0.075伏特)、高靈敏度( 42 μA-1 mM-1 cm-2 )、良好的選擇性以及穩定度。氧化銅/氧化亞銅/聚吡咯複合材料修飾紙電極應用於實際血液樣品檢測與血糖機之結果相輔合，顯示本修飾紙電極對於血糖檢測具有極大的發展潛力。本研究利用裁剪紙電極建立一個微小化的三電極系統，藉由氧化銅/氧化亞銅/聚吡咯複合材料修飾紙電極檢測β-半乳糖苷酶分解乳糖所產生的葡萄糖，造成氧化訊號強度的改變，將本電極應用於β-半乳糖苷酶之酵素活性檢測，得到之最低偵測極限為0.0053 mU mL-1，亦可以應用於大腸桿菌樣品檢測。

A simple method has been reported for the preparation of a non-enzymatic glucose sensor based on a CuO/Cu2O/Ppy composite. CuO/Cu2O/Ppy composites with an average diameter of 194 ± 6 nm coated onto Linen texture (LT) papers show glucose oxidase (GOx) like activity, which allowed for the detection of glucose at a much lower potential (-0.075 V vs. Ag/AgCl reference electrode) in alkaline solution (0.5 M NaOH), with a limit of detection (LOD) of 0.34 mM at a signal-to-noise ratio of 3. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), surface-enhanced Raman spectroscopy (SERS), and X-ray photoelectron spectroscopy (XPS) methods were used to characterize the surface morphology and composition of the CuO/Cu2O/Ppy composites. This sensor has the advantages of a low detection potential (ca. -0.075 V), high sensitivity (42 μA-1 mM-1 cm-2), selectivity, and stability. The sensor is utilized for glucose detection in blood samples, with results in good agreement with that obtained from a commercial meter. Our results reveal that the functional paper electrode holds great potential for monitoring of blood glucose levels. We also utilized CuO/Cu2O/Ppy composite modified LT paper to construct a microdevice to quantify the β-galactosidase enzymatic activity by monitoring the generation of glucose. Escherichia coli (E. coli) was employed as a model and β-galactosidase activity was induced by isopropyl-β-D-thiogalactoside (IPTG). The LOD is 0.0053 mU/mL. We show that the electrochemical assay works with the lysates of cells, allowing for the determination of β-galactosidase activity in E. coli.

謝誌 I
中文摘要 II
Abstract III
目錄 V
圖表目錄 VIII
第一章、緒論 1
1.1電化學分析法介紹 1
1.1.1 電化學系統簡介 1
1.1.2 工作電極簡介 2
1.1.3奈米材料於電分析化學介紹 3
1.1.4紙電極介紹 5
1.2 葡萄糖偵測簡介 6
1.2.1葡萄糖偵測之目的 6
1.2.2 葡萄糖之生理意義 6
1.2.3葡萄糖偵測 8
1.2.4電化學方法檢測葡萄糖 8
1.2.5 電性選擇聚合物在電化學之運用 12
1.3 Β-半乳糖苷酶偵測之簡介 12
1.3.1 β-半乳糖苷酶檢測 12
1.3.2 偵測方式 15
1.4 研究動機 16
1.5 參考文獻 17
第二章、氧化銅/氧化亞銅/聚吡咯奈米複合材料修飾紙電極製備與其電化學應用 26
2.1 前言 26
2.2 實驗材料與方法 26
2.2.1 實驗藥品與儀器 26
2.2.2 氧化銅/氧化亞銅/聚吡咯奈米複合材料修飾紙電極合成與電極之組裝 28
2.2.3 氧化銅/氧化亞銅/聚吡咯奈米複合材料修飾紙電極特性鑑定 29
2.2.4 血液樣品製備 29
2.2.5 電化學量測葡萄糖 30
2.2.6 大腸桿菌培養以及誘導基因表現 30
2.2.7 電化學量測β-半乳糖苷酶 31
2.3 實驗結果與討論 31
2.3.1 氧化銅/氧化亞銅/聚吡咯奈米複合材料修飾紙電極之合成與鑑定 31
2.3.2 氧化銅/氧化亞銅/聚吡咯奈米複合材料修飾紙電極電化學催化活性 33
2.3.3 氧化銅/氧化亞銅/聚吡咯奈米複合材料修飾紙電極於偵測葡萄糖應用 35
2.3.4 氧化銅/氧化亞銅/聚吡咯奈米複合材料修飾紙電極偵測β-半乳糖苷酶 37
2.4參考文獻 39
第三章、總結與未來展望 53

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