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研究生(外文):Yen-An Chen
論文名稱(外文):Development of a gelatin-based artificial skin phantom for electrical treatments
指導教授(外文):Wen-Pin ShihChien-Hao Liu
口試委員(外文):Chii-Wann LinPo-Jen ShihYao-Chuan Tsai
外文關鍵詞:electrotherapyartificial skin phantomlaser ablationgelatin
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An electrotherapy has become a popular physical therapy in the medical field in recent years. The wide applications include pain managements, cell repairing, and controls or treatments of various diseases. Since an electrotherapy is a relative new technology, there exists many potential applications under investigations. However, it’s still challenging to verify the effectiveness of electrotherapies on diseases because it requires clinical tests for certain illness. The improper use of the applied electricity can cause irreversible damage to patients.
The aim of this research is to develop an artificial skin phantom via MEMS fabrication and laser ablation techniques. Its mechanical and electrical properties are close to those of real human skins. In addition, this phantom is biocompatible and can be used to culture cells for electrical stimulation study.
In this research, the detailed fabrication process and the measured properties of the proposed phantom are mentioned and discussed. We also cultured cells on the phantom to investigate the biocompatibility. In the end of the thesis, we propose a potential device to investigate cell reactions to electrical stimulations for the research of

口試委員審定書 #
誌謝 i
中文摘要 ii
Chapter 1 Introduction 1
1.1 Backgrounds and motivation 1
1.2 Electrotherapy 1
1.2.1 Overview of electrotherapy 1
1.2.2 Clinical parameters to be considered for electrotherapy 3
1.3 Skin Phantoms and in vitro culture skin 5
1.3.1 Artificial skin phantoms 5
1.3.2 In vitro tissue-engineered skin model 8
1.3.3 Appropriate phantoms for electrotherapy and research objective 9
1.4 Thesis organization 11
Chapter 2 Design of artificial skin phantom including sweat pores 12
2.1 Skin Properties 12
2.1.1 Skin morphology 13
2.1.2 Sweat, sweat pores and sweat duct 14
2.1.3 Electrical properties of skin and equivalent circuit model 17
2.1.4 Mechanical properties of skin 21
2.2 Gelatin 22
2.2.1 Introduction to gelatin 22
2.2.2 Patterning technique of gelatin 23
2.2.3 Conductive gelatin 26
2.2.4 Biocompatibility of gelatin 27
2.3 Phantom Design 29
Chapter 3 Fabrication 33
3.1 Fabrication process for the SU-8 film 33
3.2 Combination of the SU-8 film with conductive gelatin 40
3.3 Patterning pores on gelatin 42
3.3.1 Thermal model for laser ablation 43
3.3.2 Process from patterning gelatin 51
Chapter 4 Phantom property test 54
4.1 Morphology observation 54
4.2 Mechanical property test 58
4.2.1 Experiment design 59
4.2.2 Measurement results and discussion 60
4.3 Electrical property test and the equivalent circuit 62
4.3.1 Experiment design 63
4.3.2 Measurement results and discussion 65
Chapter 5 Biocompatibility test and cell electrical stimulation 68
5.1 Techniques for cell culture of 3T3 cells 68
5.1.1 Materials 69
5.1.2 Cell culture method 70
5.2 Tests for biocompatibility on gelatin film 72
5.3 Design for electrical stimulating to cells on the phantom 74
Chapter 6 Conclusion and future work 77
6.1 Conclusion 77
6.2 Future Work 79

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