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研究生:陳彥安
研究生(外文):Yen-An Chen
論文名稱:研發人工皮膚仿體應用於電刺激治療
論文名稱(外文):Development of a gelatin-based artificial skin phantom for electrical treatments
指導教授:施文彬劉建豪劉建豪引用關係
指導教授(外文):Wen-Pin ShihChien-Hao Liu
口試委員:林啟萬施博仁蔡燿全
口試委員(外文):Chii-Wann LinPo-Jen ShihYao-Chuan Tsai
口試日期:2016-07-20
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:機械工程學研究所
學門:工程學門
學類:機械工程學類
論文種類:學術論文
論文出版年:2016
畢業學年度:104
語文別:英文
論文頁數:92
中文關鍵詞:電療皮膚仿體明膠雷射蝕刻
外文關鍵詞:electrotherapyartificial skin phantomlaser ablationgelatin
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近年來,電療已是普及於醫療領域的物理治療療程,它被泛用於紓解疼痛、細胞修復及各種疾病的控制或治療,例如,下背痛可用電刺激療法來治療。電療是近數十年來才興起的新興療法,還有許多應用上的可能性待研究者去探討。然而,開發新療程的過程十分艱困,每次開發都需要找到有相對應疾病的病人進行電擊測試,十分沒效率,且測試的電擊又可能導致病人不必要的傷害。因此,本研究利用微機電及雷射蝕刻製程開發一種皮膚仿體,可供電療研究者做為測試平台。此仿體的力學特性及電特性和人類的表皮層相仿,且其電特性可依需求做調整。此外,此仿體具有生物相容性,這使得研究者可以在此仿體上培養電療的目標細胞,並對其施以電擊並觀察細胞反應,進而探討電療之療效。
在本文中仿體的製程、力學及電學特性量測都被詳細討論,亦實際在仿體上操作細胞培養以討論其生物相容性。最後,本文亦提出一個可能可用來探討細胞電反應的簡單實驗設計,供電療研究者參考。

An electrotherapy has become a popular physical therapy in the medical field in recent years. The wide applications include pain managements, cell repairing, and controls or treatments of various diseases. Since an electrotherapy is a relative new technology, there exists many potential applications under investigations. However, it’s still challenging to verify the effectiveness of electrotherapies on diseases because it requires clinical tests for certain illness. The improper use of the applied electricity can cause irreversible damage to patients.
The aim of this research is to develop an artificial skin phantom via MEMS fabrication and laser ablation techniques. Its mechanical and electrical properties are close to those of real human skins. In addition, this phantom is biocompatible and can be used to culture cells for electrical stimulation study.
In this research, the detailed fabrication process and the measured properties of the proposed phantom are mentioned and discussed. We also cultured cells on the phantom to investigate the biocompatibility. In the end of the thesis, we propose a potential device to investigate cell reactions to electrical stimulations for the research of
electrotherapy.

CONTENTS
口試委員審定書 #
誌謝 i
中文摘要 ii
ABSTRACT iii
SYMBOL TABLE iv
CONTENTS v
LIST OF FIGURE ix
LIST OF TABLE xv
Chapter 1 Introduction 1
1.1 Backgrounds and motivation 1
1.2 Electrotherapy 1
1.2.1 Overview of electrotherapy 1
1.2.2 Clinical parameters to be considered for electrotherapy 3
1.3 Skin Phantoms and in vitro culture skin 5
1.3.1 Artificial skin phantoms 5
1.3.2 In vitro tissue-engineered skin model 8
1.3.3 Appropriate phantoms for electrotherapy and research objective 9
1.4 Thesis organization 11
Chapter 2 Design of artificial skin phantom including sweat pores 12
2.1 Skin Properties 12
2.1.1 Skin morphology 13
2.1.2 Sweat, sweat pores and sweat duct 14
2.1.3 Electrical properties of skin and equivalent circuit model 17
2.1.4 Mechanical properties of skin 21
2.2 Gelatin 22
2.2.1 Introduction to gelatin 22
2.2.2 Patterning technique of gelatin 23
2.2.3 Conductive gelatin 26
2.2.4 Biocompatibility of gelatin 27
2.3 Phantom Design 29
Chapter 3 Fabrication 33
3.1 Fabrication process for the SU-8 film 33
3.2 Combination of the SU-8 film with conductive gelatin 40
3.3 Patterning pores on gelatin 42
3.3.1 Thermal model for laser ablation 43
3.3.2 Process from patterning gelatin 51
Chapter 4 Phantom property test 54
4.1 Morphology observation 54
4.2 Mechanical property test 58
4.2.1 Experiment design 59
4.2.2 Measurement results and discussion 60
4.3 Electrical property test and the equivalent circuit 62
4.3.1 Experiment design 63
4.3.2 Measurement results and discussion 65
Chapter 5 Biocompatibility test and cell electrical stimulation 68
5.1 Techniques for cell culture of 3T3 cells 68
5.1.1 Materials 69
5.1.2 Cell culture method 70
5.2 Tests for biocompatibility on gelatin film 72
5.3 Design for electrical stimulating to cells on the phantom 74
Chapter 6 Conclusion and future work 77
6.1 Conclusion 77
6.2 Future Work 79
REFERENCE 81



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