臺灣博碩士論文加值系統

在快速成型程序中，利用光聚合反應能賦予產品高解析度、高機械強度以及高穩定性，高解析度更有利於快速成型產品在生物醫學領域的應用，而能被光聚合的寡聚物與單體在這些製程中扮演著重要的角色。然而，現今大部分光聚合的材料生物相容性不佳、難以在臨床上被應用。在此論文中，我們開發新型快速成型且具有良好生物相容性的紫外光固化聚合物系統，採用臨床常用的高生物相容性寡聚物進行末端改質，經過末端酯化的寡聚物呈現光反應型的官能基碳碳雙鍵(C=C)，並具有可調控的碳碳雙鍵官能基數目。

The UV-induced polymerization process used in rapid prototyping results in excellent resolution, mechanical strength and stability of products. High resolution has more conducive to product with rapid prototyping applications in the biomedical field. However, the oligomers used in this process must be UV-curable, which was difficult for most of polymers used clinically nowadays. In this study, a series of novel, biocompatible and UV-curable oligomers were developed for rapid prototyping by modifying polymers, commonly be used in clinical high biocompatible oligomer. After the esterification on the ends of oiligomers, appearing photoreactive functional group with carbon-carbon double bond (C = C), the number of reactive functional group, carbon-carbon double bond could be controlled in one oligomer.

摘要 I
Abstract II
致謝 IV
目錄 VI
圖目錄 XII
表目錄 XXII
方程式目錄 XXIV
專有名詞及縮寫 XXV
第一章 緒論 1
第二章 文獻回顧 3
2.1 3D列印的發展現況及生醫應用 3
2.1.1 未來與隱憂 4
2.2 硬化型樹脂之種類 4
2.2.1 乙烯基類反應性樹脂 5
2.3 光硬化樹脂的主要成分 6
2.3.1 寡聚物 6
2.3.2 單體 7
2.3.3 光起始劑 10
2.4 影響硬化之因素 12
2.4.1 UV燈源之強度、波長 12
2.4.2 熱 12
2.4.3 氧阻聚 12
2.4.4 層膜之厚度 13
2.4.5 寡聚物本身的性質 14
2.4.6 光起始劑之含量 14
2.5 生物相容光固化樹脂的應用 14
2.6 樹脂比例和成分對機械性質的影響 15
2.7 3D列印醫療應用的回顧 16
第三章 實驗材料與方法 18
3.1 實驗藥品 18
3.2 實驗儀器 20
3.3 實驗材料與流程 22
3.3.1 寡聚物合成 22
3.3.2 樹脂配方及混合方式 22
3.4 材料鑑定與性質檢測 26
3.4.1 傅立葉轉換紅外線光譜儀(FTIR)分析 26
3.4.2 拉伸試驗 26
3.4.1 黏度測試 29
3.4.2 降解測試 30
3.4.3 熱重量分析測試(TGA) 30
3.4.4 熱示差掃描分析儀(DSC) 31
3.4.5 接觸角量測 31
3.5 體外細胞實驗 32
3.5.1 實驗操作 32
3.5.2 光固化材料試片製作 33
3.5.3 細胞來源 35
3.5.4 細胞培養 35
3.5.5 細胞冷凍保存 36
3.5.6 細胞解凍及培養 37
3.5.7 細胞計數 37
3.5.8 粒線體活性測試 39
3.5.9 細胞骨架染色(Cytoskeleton immunostaining) 41
3.5.1 細胞的毒性測試(Annexin V-FITC/PI試劑雙染) 41
第四章 結果與討論 43
4.1 傅立葉轉換紅外線光譜儀(FTIR)分析 43
4.1.1 藉由FTIR 分析量測光固化材料的轉化率 43
4.2 反應型稀釋單體濃度對黏度的影響 48
4.3 不同溫度對黏度的影響 57
4.4 稀釋單體和寡聚物比例對光固化材料的機械性質影響 62
4.5 交聯劑對機械性質之影響 66
4.6 不同稀釋單體對材料機械性質影響 69
4.7 不同寡聚體對材料機械性質的影響 72
4.8 牙科應用在3D列印之光固化材料開發 74
4.9 重量損失量測 83
4.10 材料熱性質分析 85
4.11 接觸角量測 90
4.12 骨母細胞在光固化材料上的活性表現 93
4.13 骨母細胞在含有交聯劑的光固化材料上之活性表現 96
4.14 骨母細胞在混合不同稀釋單體的光固化材料上之活性表現 98
4.15 肌動蛋白骨架及細胞核染色 100
4.16 光固化材料的毒性測試(Annexin V-FITC/PI 試劑雙染) 105
4.17 以光固化材料製備臨床醫療裝置 113
第五章 結論 115
參考文獻 118

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