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研究生:陳威志
研究生(外文):Wei-Chih Chen
論文名稱:漆酶催化天然酚類的合成應用於染色劑具抗氧化性之研究
論文名稱(外文):Application of Laccase-catalyzed synthesis of natural phenols with antioxidative properties in coloring agent
指導教授:陳文章陳文章引用關係
指導教授(外文):Wen-Jang Chen
口試委員:蘇至善蘇文達黃孟槺
口試委員(外文):Wen-Jang ChenWen-Jang ChenWen-Jang Chen
口試日期:2016-07-19
學位類別:碩士
校院名稱:國立臺北科技大學
系所名稱:化學工程研究所
學門:工程學門
學類:化學工程學類
論文種類:學術論文
畢業學年度:104
語文別:中文
中文關鍵詞:漆酶、染料、染色性、抗氧化、酚酸、生物材料
外文關鍵詞:laccasedyestuffdyeabilityantioxidantphenolic acidbiobased materials
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本研究利用生物漆酶,催化天然植物酚酸製成染劑具染色性及抗氧化性質並符合綠色化學精神,取代過氧化氫或是對苯二胺具刺激性傳統染色劑。進一步研究漆酶對天然酚類催化情形,並得知漆酶對天然酚類催化效果各有不同,漆酶作為氧化混和酚酸阿魏酸與沒食子酸,致力於對白色毛髮進行染色性的處理。利用實驗設計來探討漆酶催化混合酚酸對毛髮染色條件:染色溫度、時間、漆酶活性、pH等因子具染色性影響。天然酚酸具有芳香環及羥基結構為良好的抗氧化劑,經由ABTS清除自由基能力檢測漆酶催化天然酚酸之抗氧化性。在60C及pH=4下使用活性30U的漆酶,阿魏酸與沒食子酸以體積比4:1混合,染色時間5小時,此條件進行染色之色差為40.03,飽和度為37.13,染色後之毛髮抗氧化能力TEAC=2.64mM高於市售染髮劑染色後之毛髮TEAC=0.48mM,使毛髮具有色彩鮮明的飽和度及色牢度。最後透過加入界面活性劑進行護髮,達到保護毛髮受損並提升色牢度。因此利用天然酚酸的抗氧化性質及生物漆酶應用於染色技術具有很大的潛能。
In line with the principles of green chemistry, the present study proposed an alternative biological method of using laccase in catalyzing natural phenols to yield coloring agents with antioxidant properties; thereby, eliminating the use of hydrogen peroxide and p-phenylenediamine (PPD). Laccase can catalyze combinations of phenolic acid, ferulic acid, and gallic acid to provide coloring agents suitable for the dyeing of gray hairs. The study was designed to examine the effect of different parameters, including dyeing temperature, time, laccase activity, and pH, on the laccase catalysis of natural phenols. Results revealed that treatment conditions influenced the catalyzing outcome. In addition, natural phenols, which contain an aromatic ring and a hydroxyl group, are capable antioxidants. The ABTS free radical scavenging test was used to probe the antioxidant properties of laccase-catalyzed phenols. Use at 60C, pH = 4 and 30U laccase activity, ferulic acid and gallic acid at a volume ratio of 4: 1 mixture of dyed 5 hours, and this condition is color difference of ΔE = 40.03, chroma of C* = 37.13, antioxidant capacity after the hair dyeing TEAC = 2.64mM higher than the commercially available hair dye hair after dyeing TEAC = 0.48mM. This allowed the hair to retain its gloss, color saturation, and color fastness. Lastly, the application of surfactants during the treatment step further prevented hair damage and promoted color fastness. Overall, these results suggest the potential of natural phenols as antioxidants as well as laccase as a catalyzer in dyeing technologies.
目 錄

摘 要 i
ABSTRACT ii
誌 謝 iv
目 錄 v
表目錄 viii
圖目錄 ix
縮寫對照表 xi
第一章 緒 論 1
1.1研究動機 1
1.2研究目的 2
第二章 文獻回顧 3
2.1染髮介紹 3
2.1.1染髮的關鍵因素 3
2.1.2毛髮構造 4
2.1.3染髮劑種類 7
2.2漆酶 8
2.3自由基的介紹 9
2.3.1自由基的定義 9
2.3.2自由基反應機制 10
2.3.3抗氧化性的酚酸化合物 10
2.4色彩學 11
2.4.1可見光與人眼 11
2.4.2色彩空間 13
2.4.3色差 14
2.5護色 14
2.5.1色牢度 14
2.5.2界面活性劑 15
第三章 實驗材料及方法 17
3.1實驗設備器材 17
3.1.1桌上型色差計 18
3.2實驗藥品與材料 20
3.3實驗流程 21
3.4實驗方法與步驟 22
3.4.1漆酶活性測試 22
3.4.2染色前處理 24
3.4.3染色與時間之關係 25
3.4.4染色與溫度之關係 25
3.4.5染色與漆酶添加量之關係 26
3.4.6染色與pH值之關係 26
3.4.7染色後毛髮經過護色之色牢度比較 27
3.4.8抗氧化能力 28
第四章 結果與討論 30
4.1漆酶催化酚酸 30
4.1.1漆酶活性測試 30
4.1.2酚酸混合的UV分析 32
4.1.3酚酸混合的FTIR分析 34
4.2毛髮染色實驗 36
4.2.1染色與染劑比例之關係 36
4.2.2染色與時間之關係 39
4.2.3染色與溫度之關係 41
4.2.4染色與漆酶濃度之關係 43
4.2.5染色與pH值之關係 45
4.3漆酶酚酸染料抗氧化能力 48
4.3.1 ABTS自由基清除能力 48
4.3.2市售染髮劑與酵素染劑之染色毛髮抗氧化能力比較 51
4.4酵素染劑經界面活性劑護色毛髮及色牢度比較 52
第五章 結論與建議 55
5.1結論 55
5.2建議 55
參考文獻 56
附錄A 60
參考文獻

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