臺灣博碩士論文加值系統

本研究透過沉澱法、改良式沉澱法及檸檬酸鈉表面處理法等三種方式完成磁性奈米偶聯體的製備，並透過FT-IR、UV-vis、XRD、TEM及雷射粒徑儀等儀器進行偶聯體特性測試，結果成功證實磁性奈米鐵氧體具備水溶、順磁性與奈米尺寸等特性並能與抗體有效的接合。
運用前面敘述三種方式製備之磁性偶聯體取代市售側流免疫層析法快篩片上膠體金偶聯體之設計，進行三聚氰胺快篩靈敏度測試，檢測極限分別為沉澱法2.4ppm、改良式沉澱法0.5 ppm及檸檬酸鈉表面處理法3.5 ppm。其中改良式沉澱法可達原廠商販售快篩片0.5 ppm之檢測極限。

In this study, the preparation of magnetic nanoparticle-antibody conjugates was carried out by three methods, such as precipitation method, improved precipitation method and sodium citrate surface treatment method.As describedThree kinds of magnetic nanoparticle conjugates were tested by instruments like FT-IR, UV-vis, XRD, TEM and Laser Particle Size Analyzer to test the characteristics.The results have successfully confirmed the preparation of the magnetic ferriteparticles are water-soluble paramagnetic nanoparticles and the effective bonding between antibodies and magnetic nanoparticles.
Using the above three methods to produce and replace the commercially available strips made with gold collidal to test the melamine sensitivity.The detection limit of the melamine rapid test was precipitation method 2.4ppm, the improved precipitation method 0.4 ppm and Sodium Citrate Surface Treatment 3.5 ppm. The improved precipitation method has successfully reachedthe commercially availablerapid test 0.5 ppm detection limit.

誌謝 ii
摘要 iii
Abstract iv
圖目錄 vii
1.緒論 1
1.1 前言 1
1.2研究動機及目的 2
1.3研究方法 4
2.文獻回顧與整理 5
2.1側流免疫層析檢測技術(Lateral Flow Immuno Assay)之簡介與應用 5
2.2偶聯體之簡介 8
2.3磁性奈米偶聯體 10
2.3.1. 磁性奈米材料之運用與特性 11
2.3.2 磁性流體之特性與製備 13
2.3.3 偶聯抗體之特性 17
2.3.4 偶聯體之製備 18
3.實驗 20
3.1實驗材料 20
3.2實驗儀器與器材 21
3.3鑑定儀器 21
3.4實驗架構與流程 22
3.4.1 偶聯體製備 22
3.4.2快篩片實際測試 26
4.結果與討論 27
4.1沉澱法製備磁性奈米偶聯體 27
4.2改良式沉澱法基質製備之偶聯體 32
4.3檸檬酸表面處理製備磁性奈米偶聯體 37
4.4 偶聯體於快篩片上之測試與運用 44
5.結論 46
參考文獻 47
自傳 55

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