臺灣博碩士論文加值系統

甜羅勒（Ocimum basillicum L.）、青莖種羅勒(Ocimum basilicum L. cv. green)、紫莖種羅勒(Ocimum basilicum L. var. purpurascens)、丁香羅勒(Ocimum gratissimum L.)四種植物在民間俗名相似，氣味相近，型態略有不同(Hussain, Anwar et al. 2008)；長久以來被民間認為是相同的物種，功用相同，但是其精油成分差異(Patel, Singh et al. 2016)與功能的不同是本研究的主要目的。經由文獻的蒐集，以預實驗蒸餾來選取最佳萃取條件，依據預實驗選取的最佳條件對栽種於相同產地的四種植物進行第二次蒸餾萃取，並利用氣相層析-質譜儀(GC-Mass)分析四種植物精油成分。然後以芳香療法界對精油化學成分常用的分類法，例如蛋型圖(簡稱茹絲的蛋)、精油四象限圖、雷達圖比較精油成分差異。最後由化學成分分析結果提出五點香氣味道與療效的關聯性假設，藉由三十位資深芳香療癒從業人員進行香氣問卷評估，印證五點假設：1.台灣產紫莖種、青莖種羅勒兩種植物精油成分相同、含量接近；2.甜羅勒與羅勒和丁香羅勒是三種香氣成分完全不同的植物；3.丁香羅勒主要精油成分是丁香酚（Eugenol），含量是四種植物裡最高，吸聞其香氣時專家們都認為最能達到舒緩肺部的效果；4.甜羅勒精油含有沉香醇(Linalool)精油化學成分，專家評估結果也都認為聞起來最能促進心神安寧、身心放鬆；5.甜羅勒的桉油醇(Eucalyptol)成分聞起來給人清新活潑之感受，沉香醇氣味有木質調沉穩且芬芳宜人，這兩種主要成分混和出來的氣味與羅勒和丁香羅勒氣味相比較，在專家評分結果中是最討人喜歡。

Ocimum basillicum L., Ocimum basilicum L. cv. Green, Ocimum basilicum L. var. Purpurascens, Ocimum gratissimum L., smell similarly, but the types are slightly different (Hussain, Anwar et al. 2008); The four plants had been considered by the people of the same species and the same function for a long time. However, the essential oils were different between the four plants (Patel, Singh et al. 2016). The main purpose of the study was to identify the difference between the four plants and the aroma functions. Through the background of the literature, five-point hypothesis were proposed to carry out in this research.
The four plants were extracted by distillation to harvest the crude extracts. And the crude extracts were analyzed by Gas Chromatography-Mass Spectrometer (GC-Mass) for the difference between the essential oil components to compare with the results of the five-point hypothesis. Sent the four kinds of plant crude extract spray and questionnaire assessment to aromatherapy experts to evaluate the fragrance and ease of mind on the points. Collected the points result to further statistical analysis and confirmed the proposed five-point hypothesis.
The result of Gas Chromatography-Mass Spectrometer (GC-Mass) Analysis of Ocimum basilicum L. var. Purpurascens and Ocimum basilicum L. cv. Green crude extracts showed that the same essential oil components. And the content were close. Through the expert evaluation results also believe that aroma ingredients of the two plants are almost the same. From the results of GC-Mass analysis of four plant extracts (Crude Extract), it was determined that sweet basil and Ocimum basilicum L. cv. Green and Ocimum gratissimum L. were completely different from the three aroma components. The results of the assessment are consistent. Eucalyptol was smelled fresh and lively feeling, Linalool was smelled like wood calm and fragrant and pleasant. The two main ingredients mixed with the smell of the Ocimum basilicum L. cv. Green and Ocimum gratissimum L. odor phase Comparison, in the expert score results were the most pleasing. The result of Eugenol in the gas chromatography - mass spectrometer (GC-Mass) analysis showed that it was the main essential oil component of Ocimum gratissimum L., the highest of the four plants. So, experts believe that the aroma of Ocimum gratissimum L. was the best to achieve the effect of soothing the lungs. Gas Chromatography-Mass Spectrometer (GC-Mass) analysis of the sweet basil crude extract showed that it contained Linalool, essential oil ingredients. In Expert evaluation results, sweet basil was also thought that the most likely to promote peace of mind, physical and mental relaxation. Gas chromatography-mass spectrometry (GC-Mass) analysis of the essential oil compositions of the results were fully confirmed with the proposed five-point hypothesis. The statistical analysis of the expert scores was also fully confirmed with the proposed five-point hypothesis, and matched the results of the GC-Mass analysis.

目 錄

摘 要 I
ABSTRACT II
誌 謝 IV
目 錄 V
表 目 錄 VII
圖 目 錄 VIII

第一章 緒 論 1
第一節 研究背景及動機 1
第二節 研究目的 2
第三節 研究範圍與限制 2
第四節 研究架構 2

第二章 文獻探討 4
第一節 甜羅勒簡介 4
第二節 青莖種羅勒與紫莖種羅勒簡介 5
第三節 丁香羅勒簡介 7
第四節 其他分類方法的羅勒 7
第五節 常見的精油萃取方法 8
第六節 芳香療癒 9
第七節 香氣成分探討 10
第八節 不同羅勒品種的差異性研究 13

第三章 實驗方法與材料、設備 15
第一節 栽種與採收 15
第二節 實驗儀器與設備 16
第三節 第二次蒸餾的萃取步驟 18
第四節 GC-MS分析條件 18
第五節 香氣療癒評估 18

第四章 研究結果與討論 20
第一節 栽種與採收 20
第二節 第一次萃取：最適萃取條件選取 21
第三節 第二次蒸餾GC-MS分析與香氣療癒假設 24
第四節 四種羅勒與國際ISO比較 27
第五節 四種羅勒的茹絲蛋型圖與精油四象限圖 32
第六節 香氣評估統計結果 39
第七節 網路謠傳台灣九層塔 39

第五章 結 論 40

參考文獻 41

附錄一 44

參考文獻

中央研究院 台灣本土植物資料庫. 數位典藏計畫.
王人澍, et al. (2009). "史蒂文生氏症候群合毒性皮膚上皮壞死中西醫結合治療病例." 中西整合醫學雜誌 11(2): 21-27.
王禮陽 (1994). 台灣果菜誌, 時報文化出版企業有限公司.
吳正忠 (2009). "九層塔 (Ocimum basilicum L.) 揮發性成分及抑菌性之探討." 屏東科技大學食品科學系所學位論文: 1-75.
吳佳玲, et al. (2015). "古今中外芳香療法之探討." 長庚科技學刊(22): 119-129.
李明銀, et al. (2008). "欧洲罗勒的引种栽培研究." 綿陽師範學院學報 27(5): 54-59.
李晶晶, et al. (2008). "细辛的现代药理研究概况." 中华中医药学会中药基础理论分会首届临床中药学学术研讨会论文集 92.
李靖芳 (2000). "芳香療法大百科." 世茂, 台北新店: 34-36.
易光輝, et al. (2014). 精油之化學基礎與實務應用, 華杏.
林耿志 (2012). "七層塔水萃取物透過誘發細胞週期停止抑制肝癌細胞生長." 中興大學生命科學系所學位論文: 1-64.
林榮貴, et al. (1993). "臺灣民間藥七層塔之保肝藥效評估." 嘉南學報, 19 期:: 21-32.
邱年永 and 張光雄 (1995). 原色臺灣藥用植物圖鑑(I), 南天.
柯美慧 (2010). "七層塔水萃物減少人類肺腺癌 A549 細胞株 MMP-2 與 u-PA 蛋白表現之訊息路徑探討." 中山醫學大學生化暨生物科技研究所學位論文: 1-39.
洪銓成 (2006). "七層塔水萃取物保肝功能之評估." 中山醫學大學生化暨生物科技研究所學位論文: 1-91.
胡芳晴 (2009). "評估乙醯胺基苯酚誘導慢性肝損傷之動物模式並以此模式探討七層塔精油之護肝功效." 臺灣大學食品科技研究所學位論文: 1-139.
范文穗, et al. (2015). "耐多药肺结核治疗中常见药物不良反应发生分析." 今日药学(6): 446-449.
張定霖, et al. (2003). 香藥草植物圖鑑, 行政院農業委員會種苗改良繁殖場.
張曼 (2004). "丁香酚对血压正常大鼠心血管的作用." 国外医药: 植物药分册 19(5): 209-209.
張隆仁 and 邱建中 (2003). "羅勒 (Basil)-芳香成分來源, 香辛調味及景觀用途之植物." 臺中區農業專訊(43): 3-6.
張隆仁, et al. (2010). "藥用植物之開發與應用." 臺中區農業改良場特刊 (101): 204-208.
莊雯綾 (2008). "利用外表性狀與 ISSR 分子標誌評估羅勒屬之遺傳變異." 中興大學農藝學系所學位論文: 1-40.
陳千純, et al. (2016). "探討市售九層塔 (Ocimum basilicum L.) 莖與葉之化學組成與釐清是否含有黃樟素 (Safrole) 與丁香酚 (Eugenol) 等致癌成分." 弘光學報(78): 289-298.
陶弘景 (2015). 本草經集注, 朔雪寒.
楊天鹏, et al. (2007). "丁香酚通过嗅觉通路改善昆明鼠学习记忆的机制." 中國康復醫學雜誌 22(6): 487-489.
楊遠波, et al. (1999). "台灣維管束植物簡誌第二卷 行政院農業委員會."
溫佑君 (2003). "精油圖鑑." 商週出版社, 第一版.
葉茂生 (1999). "台灣山地作物資源彩色圖鑑." 樹豆. P11e 台灣省政府農林廳. 南投.
趙秀麗, et al. (2006). "丁香酚前体药物的合成及其微乳的制备." 以岭医药杯” 第八届全国青年药学工作者最新科研成果交流会论文集.
蔡月夏 (2002). "香草植物 羅勒的栽培與利用." 花蓮區農業專訊(41): 8-8.
鄭武燦 (2000). 台灣植物圖鑑, 茂昌圖書有限公司.
繆希雍 (1982). 神農本草經疏, 商務.
謝文全 (2002). "臺灣常用藥用植物圖鑑." Ι, 臺北: 114.
謝文全, et al. (2003). "台灣常用藥用植物圖鑑 第三冊."
蘇熙元 (2010). "探討七層塔水萃物對 LPS 所誘發上皮細胞發炎之抗發炎機制." 中山醫學大學生化暨生物科技研究所學位論文: 1-63.
Ali, B., et al. (2015). "Essential oils used in aromatherapy: A systemic review." Asian Pacific Journal of Tropical Biomedicine 5(8): 601-611.
Azimova, S. S. and A. I. Glushenkova (2012). Ocimum gratissimum L. Lipids, Lipophilic Components and Essential Oils from Plant Sources, Springer: 448-449.
Burzo, I. and D. Mihaiescu (2005). "CONTRIBUTIONS TO THE DATA CONCERNING PHYSIOLOGICAL AND BIOCHEMICAL PROCESSES OF OCIMUM BASILICUM L." Analele Stiintifice ale Universitatii" Al. I. Cuza" din Iasi 51: 47.
Darrah, H. H. (1980). "cultivated basils."
Fiori, A., et al. (2000). "Antifungal activity of leaf extracts and essential oils of some medicinal plants against Didymella bryoniae." Journal of Phytopathology 148(7‐8): 483-487.
Grayer, R. J., et al. (1996). "Infraspecific taxonomy and essential oil chemotypes in sweet basil, Ocimum basilicum." Phytochemistry 43(5): 1033-1039.
Javanmardi, J., et al. (2002). "Chemical characterization of basil (Ocimum basilicum L.) found in local accessions and used in traditional medicines in Iran." Journal of agricultural and food chemistry 50(21): 5878-5883.
Juergens, U., et al. (2003). "Anti-inflammatory activity of 1.8-cineol (eucalyptol) in bronchial asthma: a double-blind placebo-controlled trial." Respiratory medicine 97(3): 250-256.
Karapinar, M. and Ş. E. Aktuǧ (1987). "Inhibition of foodborne pathogens by thymol, eugenol, menthol and anethole." International Journal of Food Microbiology 4(2): 161-166.
Khalid, K. A. (2006). "Influence of water stress on growth, essential oil, and chemical composition of herbs (Ocimum sp.)." Int. Agrophys 20(4): 289-296.
Knobloch, K., et al. (1989). "Antibacterial and antifungal properties of essential oil components." Journal of Essential Oil Research 1(3): 119-128.
Kothari, S., et al. (2004). "Essential oil yield and quality of methyl eugenol rich Ocimum tenuiflorum Lf (syn. O. sanctum L.) grown in south India as influenced by method of harvest." Journal of Chromatography A 1054(1): 67-72.
Lewinsohn, E., et al. (2000). "Biosynthesis of estragole and methyl-eugenol in sweet basil (Ocimum basilicum L). Developmental and chemotypic association of allylphenol O-methyltransferase activities." Plant Science 160(1): 27-35.
Mondello, L., et al. (2002). "Studies on the essential oil‐bearing plants of Bangladesh. Part VIII. Composition of some Ocimum oils O. basilicum L. var. purpurascens; O. sanctum L. green; O. sanctum L. purple; O. americanum L., citral type; O. americanum L., camphor type." Flavour and fragrance journal 17(5): 335-340.
Nakamura, C. V., et al. (1999). "Antibacterial activity of Ocimum gratissimum L. essential oil." Memórias do Instituto Oswaldo Cruz 94(5): 675-678.
Ogendo, J., et al. (2008). "Bioactivity of Ocimum gratissimum L. oil and two of its constituents against five insect pests attacking stored food products." Journal of Stored Products Research 44(4): 328-334.
Özgen, U., et al. (2006). "Antioxidant properties of some medicinal Lamiaceae (Labiatae) species." Pharmaceutical biology 44(2): 107-112.
Paton, A. (1992). "A synopsis of Ocimum L.(Labiatae) in Africa." Kew Bulletin: 403-435.
Pessoa, L., et al. (2002). "Anthelmintic activity of essential oil of Ocimum gratissimum Linn. and eugenol against Haemonchus contortus." Veterinary Parasitology 109(1): 59-63.
Purkayastha, J. and S. C. Nath (2006). "Composition of the camphor-rich essential oil of Ocimum basilicum L. native to Northeast India." Journal of Essential Oil Research 18(3): 332-334.
Sajjadi, S. E. (2006). "Analysis of the essential oils of two cultivated basil (Ocimum basilicum L.) from Iran." DARU Journal of Pharmaceutical Sciences 14(3): 128-130.
Shetty, S., et al. (2008). "Evaluation of antioxidant and wound healing effects of alcoholic and aqueous extract of Ocimum sanctum Linn in rats." Evidence-Based Complementary and Alternative Medicine 5(1): 95-101.
Simon, J. E., et al. (1992). "Water stress-induced alterations in essential oil content and composition of sweet basil." Journal of Essential Oil Research 4(1): 71-75.
Valnet, J. (2012). The practice of aromatherapy, Random House.
Wakazono, H., et al. (1998). "Immunochemical identification of hepatic protein adducts derived from estragole." Chemical research in toxicology 11(8): 863-872.
Xu, H., et al. (2007). Other Kinds of Medicine. Chinese Medicine in Pictures 1 (Zhongcaoyao Tupu 1), Southern Publishing & Media Co., Ltd. 330: 330-354.