網版印刷碳電極（Screen-printed carbon electrodes，簡稱SPCE），具成本低廉、製程簡單、造型自由和一次性使用等優點，在現今的學術領域以及商業化傳感器中佔有很重要的地位。但SPCE的碳膠中為了使碳粉粉末附著於基版上使用了許多疏水性的高分子膠而影響電極的再現性及靈敏度。
在本篇研究中，將分別探討Poly-L-lysine、Polymelamine以及Gold nanoparticles修飾於電極表面，探討修飾膜對SPCE電化學特性的影響及電極保存的穩定性。篩選出最適當的基材並探討應用於瘦肉精的偵測之可行性。

Screen-printed carbon electrodes (SPCE) play an important role in academic research and development of commercial sensors because of the advantages of low cost, simple process, freedom of modeling and one-time use. However, carbon powder was attached to the electrode substrate of SPCE with the use of hydrophobic macro molecular, adhesive, affecting the reproducibility and sensitivity of SPCE.
In this study, poly-L-lysine, poly-melamine, and gold nanoparticles were modified on the surface of the electrode to change the electrochemical properties of SPCE and storage stability. The feasibility study of β-agonist detection was investigated using the modified SPCE.

中文摘要............................................................A
Abstract............................................................B
目次................................................................C
圖次................................................................E第一章　研究背景與目的..............................................1
1.1 研究背景......................................................1
1.2 文獻回顧......................................................4
1.3 研究目的......................................................8
第二章 實驗部分.....................................................9
2.1 藥品..........................................................9
2.2 儀器.........................................................10
2.3 藥品配製.....................................................11
2.4 實驗步驟.....................................................13
2.5 偵測Rac.....................................................15
第三章 結果與討論..................................................16
3.1 網版印刷碳電極預處理.........................................16
3.2 SPCE*表面修飾................................................ 18
3.2.1 SPCE*-PLL的修飾..........................................18
3.2.2 SPCE*-pM的修飾...........................................22
3.2.3 SPCE*-AuNP的修飾.........................................24
3.3 SPCE*修飾後的穩定性..........................................33
3.4 自製與市售奈米金電極比較.....................................36

3.5 SPCE*對Rac偵測的最適化修飾基材探討..........................40
3.5.1 SPCE*-pM偵測Rac..........................................40
3.5.2 SPCE*-AuNP偵測Rac........................................42
3.6 探討Rac偵測的最適化pH值.....................................45
3.6.1 SPCE*偵測................................................46
3.6.2 SPCE*-AuNP偵測...........................................49
3.7 SPCE*-AuNP對Rac的選擇性初步探討.............................51
第四章 結論................................................ ..... ..53
參考文獻................................................... ..... ..54

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