臺灣博碩士論文加值系統

為了修復人體骨缺損，以骨植體做為骨替換材料已成為不可或缺的生醫材料。其為了加速骨骼和身體組織骨癒合進行骨植體表面修飾的研究越來越多。其也發現當骨植體植入人體時，骨癒合的成功取決於細菌感染有關。近年來，氧化鋯骨植體被廣泛應用，然而由於惰性表面，氧化鋯在植入後的生物活性差值入後材料與骨組織間無法產生化學結合。根據文獻，本實驗使用氧化鋯作為表面修飾塗層基材，其表面添加鈣矽其幫助骨骼癒合，使用硝酸銅5%、2%、1%，硝酸銀0.001%，幾丁聚醣1%、0.5%、0.1%，幾丁寡醣10%、5%、1%，幾丁聚醣四級銨1%加入不同比例的銨劑，作為抑菌劑以抑制細菌生長。通過SEM、XRD、FTIR、XPS和微生物進行分析。實驗結果顯示，各層元素分析在XRD與FTIR可發現Zircona與Poly-dopamine和鈣矽元素，無法顯示抑菌層元素，但從XPS分析能確定表面塗層元素，後續塗層抑菌測試透過存活率以及細菌貼附表面形貌圖兩項分析得到一致的結果，各組培養24小時均能有效抑制細菌生長更隨著濃度提高抑制細菌越高，與控制組相比具有顯著差異，其幾丁聚醣四級銨與幾丁聚醣兩組結果顯示較相似的。幾丁寡醣10% 與0.5%幾丁聚醣相似，幾丁聚醣四級銨與幾丁聚醣提供比幾丁寡醣具有更好抑制細菌效果，硝酸銀相似於硝酸銅組，其抑制細菌效果比幾丁聚醣四級銨與幾丁聚醣好。

The bone implants has become an indispensable material for reconstruction of cortical bone. A variety of surface modification techniques on implants have been developed to achieve the accelerated bone healing. However, the success of bone healing depends on bacterial infection when implant is implanted into human body. Recently, zirconia material is widely used in clinical applications, but it has a poor antibacterial efficacy. In this study, copper nitrate, silver nitrate, chitosan polysaccharide (CS), chitosan oligosaccharide (COS), and chitosan quaternary ammonium (QTS) were used as antibacterial top layers whereas calcium silicate interlayer was used for osteogenesis. The characterization of the coated layers on the ZrO2 substrates was performed, including SEM, XRD, FTIR, XPS, and antibacterial test. The experimental results showed that the use of XRD and FTIR was difficult to clarify coating composition, whereas the XPS analysis could determine the surface coating differences. The antimicrobial analyses including viability and morphology examinations consistently demonstrated the antibacterial efficacy of the used coating layers. Each group of antibacterial agents cultivated for 24 hours can effectively reduce the growth of bacteria. As expected, the higher concentration of the agent, the more effective was found. It has quite difference as compared to control group. The antibacterial effectiveness of QTS and CS were similar to each other, and COS had a lower effectively than CS. The Ag and Cu, layer had a greater antibacterial efficacy than QTS and CS.

誌謝...........................................................I
摘要..........................................................II
ABSTRACT....................................................III
目錄..........................................................IV
圖目錄............................................... .......VII
表目錄......................................................VIII
第一章
緒論................................................... .......1
1-1研究背景....................................................1
1-2研究目的............................................. ......2
第二章 文獻回顧............................................... 3
2-1 生醫材料.................................................. 3
2-2 骨替代材料................................................ 5
2-3 骨生醫材料................................................ 6
2-4 植體表面修飾.............................................. 6
2-5 植體表面修飾方法.................................... .......7
2-5-1 電漿表面處理...................................... ......9
2-5-2 滴塗層................................................. 10
2-6 骨替代目標............................................... 10
2-7 表面修飾材料....................................... ......12
2-7-1聚多巴胺.......................................... ......12
2-7-2 幾丁聚醣......................................... ......13
2-7-3 幾丁聚醣衍生物................................... ......15
2-7-4 矽酸鈣................................................. 16
2-7-5 銅、銀離子............................................. 17
第三章 實驗材料與方法................................... ......19
3-1 人工植體表面修飾................................... .......19
3-2 材料合成................................................. 20
3-2-1 聚多巴胺層............................................. 20
3-2-2 矽酸鈣層........................................ .......20
3-2-3 抑菌層.......................................... .......21
3-3 基本性質分析............................................. 22
3-3-1 粉體分析............................................... 22
3-3-2 液體分析............................................... 23
3-3-3 塗層表面分析......................................... ..23
3-3-4 塗層微生物測試........................................ .24
第四章 實驗結果............................................. .26
4-1 粉體分析FTIR............................................. 26
4-2 液體分析-微生物試驗....................................... 27
4-2-1 混濁度測試............................................. 27
4-2-2 生存率測試............................................. 28
4-2-3 培養基測試............................................. 29
4-3 塗層表面元素分析.......................................... 31
4-3-1 表面形貌............................................... 31
4-3-2 XRD............................................ .......34
4-3-3 FTIR ................................................. 35
4-3-4 XPS................................................... 36
4-3-5塗層抑菌性.............................................. 37
第五章 討論.................................................. 40
第六章 結論.................................................. 42
參考文獻..................................................... 43

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