本研究中利用化學沉澱及離子交換兩種方式對發酵液中的己二烯二酸進行分離。在化學沉澱部分，探討pH值、靜置時間及活性碳比例對產率及純度之關係。在pH 2.5，靜置時間30 min以上且活性碳添加量為沉澱物重量之12.5%時，己二烯二酸產率可達到85%，純度達到96%。離子交換方面，利用批次實驗篩選最適合之樹脂，探討離子型態、己二烯二酸濃度及pH值對吸附之影響，發現氯型在保存與實驗上具有優勢，而Amberlite IRA 400樹脂最大吸收量為每克樹脂可吸附110毫克己二烯二酸，且吸附模式符合Freundlich方程式。並比較溫度對吸附之影響與樹脂選擇性，在溫度25℃與35℃其吸附能力皆相當穩定；在溶液中同時存在苯甲酸或琥珀酸時，其吸附能力也不受其影響。管柱實驗部分，探討不同流速對吸附之影響，最終流速選擇3.84 BV/hr，吸附率可達到44.47%。脫附劑則比較不同濃度NaCl對樹脂之脫附效果，最終選定0.5M NaCl作為脫附劑，其脫附率可達到88%。

In this study, chemical precipitation and ion exchange were used to the recovery of muconic acid from the fermentation broth. To investigate the effects of the experimental conditions on the yield and purity in chemical precipitation treatment, we examined the pH value, the precipitation time and the used weight ratio of the activated charcoal to the precipitate in the operation. The results show that the maximum yield and purity achieved to 85% and 96% while the manipulated pH was at 2.5, the precipitation time was operated more than 30 minutes and the used weight ratio of the activated charcoal to the precipitate was controlled at 12.5%.
In order to find the suitable resin for the recovery of muconic acid, we checked the ionic forms of different resin to identify the influence of the inlet muconic acid concentrations and the operating pH values. The results showed that Cl form had more advantages than OH form in experimental operation and the storage of resins. We also found the Amberlite IRA 400 had the highest adsorption capacity of 110 mg muconic acid/g resin in this system. In addition, the muconic acid adsorption behavior by using IRA 400 resin followed the Freundlich isotherm model. Furthermore, Amberlite IRA 400 not only had a stable adsorption capacity at 25℃-35℃, but also had a good selectivity to muconic acid recovery while other organic acids in solution simultaneously.
The breakthrough curve was obtained by using Amberlite IRA 400 as an adsorbent and NaCl as a desorbent. After investigation of the different flow rates and the desorbent concentrations, it could be found the adsorption percentage was 44.47% and the desorption percentage was 88% by carrying out a optimal flow rate of 3.84 BV/hr and desorbent concentration of 0.5 M.

中文摘要 i
Abstract ii
誌謝 iii
目錄 iv
圖目錄 vi
表目錄 viii
縮寫與代號 ix
第一章 緒論 1
1.1前言 1
1.2己二烯二酸介紹 4
1.2.1己二烯二酸的特性與發現 4
1.2.2己二烯二酸的生產方法 6
1.2.3己二烯二酸的市場與應用 10
1.3研究動機與目的 11
第二章 文獻回顧 12
2.1發酵液中有機酸的回收 12
2.1.1溶劑萃取與電透析 12
2.1.2化學沉澱 13
2.1.3吸附 15
2.1.4離子交換 18
2.2不同分離方式比較 20
第三章 實驗材料與方法 22
3.1實驗藥品 22
3.2實驗儀器 23
3.3分析方法 24
3.4實驗步驟 28
3.4.1醱酵實驗與醱酵液預處理 28
3.4.2化學沉澱法實驗步驟與條件 28
3.4.3離子交換法實驗步驟 29
3.5實驗參數定義與公式計算 31
3.5.1各實驗參數定義 31
3.5.2本研究使用之離子交換樹脂與其代號 34
第四章 實驗結果與討論 36
4.1醱酵實驗 36
4.1.1性質鑑定 37
4.2化學沉澱與吸附 39
4.2.1酸沉澱 39
4.2.2活性碳吸附 43
4.3離子交換 45
4.3.1離子交換靜態吸附 45
4.3.2離子交換動態吸附 53
第五章 結論 59
第六章 未來展望 61
參考文獻 62

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