臺灣博碩士論文加值系統

中文摘要
蝦紅素Astaxanthin已證實為有效抗氧化及保健功用的食品，故如何在眾多來源中找尋可提取最高蝦紅素濃度、成本便宜，促為使製程簡單化，為本研究主要課題。本文中係以蘋果螺卵Pomacea canaliculata為主軸，利用類胡蘿蔔素蛋白質加入乙醇使蛋白質與蝦紅素分離，探討乙醇濃度、攪拌時間，再使用抽氣過濾裝製分離固體蛋白質與液體蝦紅素乙醇溶液。實驗結果，可成功建立螺卵中萃取出蝦紅素之生產策略。過程較紅藻類、酵母菌、海產廢棄物簡單。且能夠提煉出更具成本優勢之蝦紅素，濃度上也非常突出，後續動物試驗也都顯示出，此種方法所提煉出之蝦紅素完全可以應用於飼料、食品及保養品上面。

關鍵字：蝦紅素、蘋果螺卵、萃取

Abstract
Astaxanthin has been verified as food with effective anti-oxidation and health function. This article will be presenting how to search for the economical way of extracting the highest Astaxanthin from various sources and simplification of the production. Pomacea canaliculata will be the main source, the use of carotenoid-protein by adding ethanol to separate protein and Astaxanthin, investigating the concentration of ethanol, duration of mixing, then using suction filtration to separate solid protein and Astaxanthin ethanoate solution. Successful experiment result could depend on the production strategy of extracting Astaxanthin from Pomacea canaliculata. The process is simpler for dulse, yeast, waste products and seafood. This method of extracting Astaxanthin is more profitable and its concentration is also prominent. The following-up animal testing also showed that the extracted Astaxanthin from this method could be fully applied to feeds, food and skin products.

Keywords: Astaxanthin、Pomacea canaliculata、Extracted

目錄
明志科技大學碩士學位論文口試委員審定書 i
中文摘要 ii
Abstract iii
目錄 iv
圖目錄 vi
第一章 緒論 1
第二章 文獻回顧 3
2.1 前言 3
2.1.1 蝦紅素來源 3
2.1.2 紅球藻 3
2.1.3 紅酵母菌 4
2.1.4 海產廢棄物 4
2.1.5 研究方向 4
2.2 蝦紅素物理化學性質 5
2.3 蝦紅素萃取 6
2.3.1 蝦卵取得 6
2.3.2 蛋白質變性溶劑 6
2.3.3 超臨界萃取 6
第三章 方法結果與討論 7
3.1 最佳萃取重量比例 7
3.2 最佳溶劑萃取比例 9
3.3 最佳時間萃取試驗 10
3.4 油溶性萃取試驗 12
3.5 抗腫瘤試驗 13
3.6 美白試驗 14
第四章 結果與展望 15
參考文獻 16

參考文獻
Baldea, I.; Mocan, T.; Cosgarea, R. The role of ultraviolet radiation and tyrosine stimulated melanogenesis in the induction of oxidative stress alterations in fair skin melanocytes. Exp. Oncol. 2009, 31, 200–208.
Briganti, S.; Picardo, M. Antioxidant activity, lipid peroxidation and skin diseases. What’s new. J. Eur. Acad. Dermatol. Venereol. 2003, 17, 663–669.
Weinstock, M. A. Early detection of melanoma. J. Am. Med. Assoc. 2000, 284, 886–889.
Halachmi, S.; Gilchrest, B. A. Update on genetic events in the pathogenesis of melanoma. Curr. Opin. Oncol. 2001, 13, 129–136.
Jemal, A.; Devesa, S. S.; Hartge, P.; Tucker, M. A. Recent trends in cutaneous melanoma incidence among whites in the United States. J. Natl. Cancer Inst. 2001, 93, 678–683.
R. Todd Lorenz and Gerald R. Cysewski Commercial potential for Haematococcus microalgae as a natural source of astaxanthin. TIBTECH APRIL. 2000, 18, 160-167
Tran, H.; Chen, K.; Shumack, S. Epidemiology and aetiology of basal cell carcinoma. Br. J. Dermatol. Supplement 66. 2003, 149, 50–52.
White, N.; Knight, G. E.; Butler, P. E.; Burnstock, G. An in vivo model of melanoma: Treatment with ATP. Purinergic Signalling. 2009, 5, 327–333.
Wang, H. M.; Chen, C. Y.; Wen, Z. H. Identifying melanogenesis inhibitors from Cinnamomum subavenium with in vitro and in vivo screening systems by targeting the human tyrosinase. Exp. Dermatol. 2011, 20, 242–248.

Wang, H. M.; Pan, J. L.; Chen, C. Y.; Chiu, C. C.; Yang, M. H.; Chang, H. W.; Chang, J. S. Identification of anti-lung cancer extract from Chlorella vulgaris C_C by antioxidant property using supercritical carbon dioxide extraction. Process Biochem. 2010, 45, 1865–1872.