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研究生:李宗翰
研究生(外文):Tzung-Han Lee
論文名稱:禽畜糞堆肥病原微生物安全性研究
論文名稱(外文):Study on pathogenic microbial safety of animal manure composts
指導教授:譚鎮中譚鎮中引用關係
口試委員:廖乾華黃政華
口試日期:2017-07-31
學位類別:碩士
校院名稱:國立中興大學
系所名稱:土壤環境科學系所
學門:農業科學學門
學類:農業化學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:中文
論文頁數:58
中文關鍵詞:病原微生物禽畜糞堆肥有機
外文關鍵詞:PathogenManureorganic
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近年來有機資材的使用大幅增加,處理不當的有機質肥料可能含有大量的病原微細菌,經施用後可能最影響到土攘及農作物而造成環境衛生的問題,因此本研究的目的主要是1、堆肥化過程中病原指標微生物變化情形,2、觀察施用不同有機質肥料對土壤及植體中病原指標微生物之影響,以作為日後提供政府規範堆肥中病原菌狀況之根據。試驗結果顯示堆肥化過程確實能降低病原指標菌含量,最後一次分析結果顯示大腸桿菌群菌數B(牛糞:穀殼:米糠=4.5:4.5:1 )(3.1×104 cfu g-1)>A(牛糞:豬糞:雞糞:穀殼:米糠=1:1:1:1:1 )(5.5×103 cfu g-1)>C(雞糞:穀殼:米糠=4:5:1 )(1.1×101 cfu g-1),糞大腸桿菌菌數A(3.6×102 cfu g-1)≒B(1.7×102 cfu g-1)>C(nd),沙門氏菌菌數B(2.1×103 cfu g-1)>C(2.9×102 cfu g-1) ≒ A(2.4×102 cfu g-1 ),顯示經過堆肥處理,確實可降低堆肥中病原微生物數量,但仍要進行適當的翻堆才可完全殺菌。另外,隨著禽畜糞堆肥的施用,土壤及植體中的大腸桿菌群、糞大腸桿菌及沙門氏菌菌數含量確實有逐漸上升的趨勢,反之CK(5號複合肥料)則沒有病原菌上升的情況,顯示隨著施用禽畜糞堆肥確實可能造成土壤及植體病原微生物數量的上升。
Application of organic fertilizers such as compost in crop production has been increasing drastically. However, pathogenic bacteria in composts may transfer into plant tissue and cause severe public health problems if the composts are not treated properly. The primary purpose of this study was to observe the variations of pathogenic microorganisms during the composting process and in soil and plant after application of different manures. The result showed that the composting process could reduce the number of pathogens in manures. Composting treatment B ranked first in the final number of coliforms after composing, followed by treatment A and C. Composting treatment A ranked first in the final number of fecal coliform after composing, followed by treatment B and C. Composting treatment B ranked first in the final number of Salmonella after composing, followed by treatment C and A. The amount of pathogens in soils and plant tissues increased with increasing of application amounts of manures.
目錄………………………………………………………….………….け
摘要……………………………………….………………...…………..こ
Abstract…………………………...…………………………..…………ご
表次…………………………………………………………….……….し
圖次………………………………………………..……………………じ

壹、 前言…………………………………..……………………………1
貳、 前人研究…………………………………………………………..3
一、 指標微生物…………………………………………………..3
(一)定義………………………………...……………………3
(二)特性…………………………………...…………………3
(三)大腸桿菌群………………………...……………………3
(四)糞大腸桿菌………………………………...……………4
(五)沙門氏菌……………………………………...…………4
二、堆肥安全性研究…………………………………....…………5
三、堆肥中病原微生物存活之研究………………………………8
四、國外堆肥病原微生物法規……………………………………9
參、材料與方法………………………………………..……….………10
一、堆肥化對病原指標微生物變化之影響………………………10
(一)試驗材料……………………………………….………10
(二)試驗處理……………………………………….………10
(三)試驗方法……………………………………….………11
(四)調查項目……………………………………….………11
(五)堆肥樣品中病原指標菌之測定……………….………11
(六)有機質肥料理化性質分析…………………….………13
二、施用堆肥對土壤及植體病原指標微生物之影響…………...19
(一)試驗作物……………………………………….………..19
(二)試驗處理……………………………………….………..19
(三)施肥量………………………………………….………..19
(四)調查項目……………………………………….………..20
(五)堆肥樣品中病原指標菌之測定………………….……..20
(六)有機質肥料理化性及成分分析………………….……..20
(七)土壤理化性質分析…………………………….………..21
肆、結果…………………………………………………….………….26
一、堆肥化對病原指標微生物變化之影響…………………….26
二、施用禽畜糞堆肥對於土壤及植體之影響………………….32
伍、討論……………………………………………….…….…………43
一、堆肥化對病原指標微生物變化之影響…………………….....43
二、施用禽畜糞堆肥對於土壤及植體之影響………………...…..45
陸、結論………………………………………………………………..48
柒、參考文獻…………………………………………………………..49
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