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研究生(外文):Kai-Jei Zhang
論文名稱(外文):Investigation of Fine Airborne Particulate (PM2.5) Induces DNA StrandBreakesin Human Bronchus Cell Lines (BEAS-2B)
外文關鍵詞:PM2.5DNA break
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There are many families in Taiwan that have a tradition of incense and worship. This study uses five different sources of incense A, B, C, D, and E in the laboratory to burn the air sampler to collect the burning line for ten minutes. Effects of the mixture of organic solution extract and aqueous solution on the cytotoxicity, oxidative stress and DNA damage of normal human lung epithelial cells. Where A is mixed incense, B, C, E for the sink sandalwood, D is black incense.
In the cytotoxicity test is the use of dyeing count the survival rate of the way, the 24 hours when the toxicity from large to small for the A, C, D, B, E; in the active oxygen test 30 minutes when the oxidation pressure from large to small For B, D, C, E, A, where the oxidation pressure of A is about 1.5 times that of negative control group, B is about 2.67 times, C is about 2 times, D is about 2.4 times and E is about 1.7 times. The reason for the difference may be that the amount of substances in the line that can cause the action of the active oxygen molecules is not so fast, so the amount produced in a short time is not much.
DNA damage was measured by comet assay. The test time was 24 hours. The DNA damage was from B to A, E, C, D, A was about 14 times higher than that of control group, B was about 35 times, and C About 12 times, D about 1.35 times, and E is about 11.5 times. 
摘要 i
Abstract ii
縮寫表 iii
目錄 iv
圖目錄 vii
表目錄 ix
第一章 1
1.1 研究緣起 1
1.2研究目的 1
第二章文獻回顧 2
2.1空氣懸浮微粒(PM) 2
2.1.1分類 2
2.1.2成分組成 2
2.2可能吸附之物質與總懸浮微粒 介紹可能吸附在空氣懸浮微粒上的物質和懸浮顆粒有關之實驗研究。 4
2.2.1五氯苯酚(PCP) 4
2.2.2萘醌類衍生物(Nap) 4
2.2.32,3,7,8-四氯二苯並對二噁英(TCDD) 4
2.2.4總懸浮微粒(TSP) 5
2.3空氣懸浮微粒及奈米顆粒對人及生物造成之影響 6
2.3.1細胞毒性 6
2.3.2活性氧分子 7
2.3.3DNA損害 9
2.3.4健康之危害效應 9
2.4 BEAS-2B細胞相關 10
2.5萃取方法 11
第三章實驗設計架構與假設 12
3.1研究假設 12
想了解不同成分的線香樣本燃燒後的PM2.5對細胞毒性、氧化壓力、DNA損害等實驗上能否判斷出何者是最需要被注意的。 12
3.2實驗設計架構 13
第四章實驗材料與方法 14
4.1 實驗設備與材料 14
4.1.1 藥品試劑 14
4.1.2 細胞株 16
4.1.3實驗設備 16
4.2 實驗方法 19
4.2.1細胞培養 19
4.2.2 濾紙萃取 20
4.2.3細胞毒性測試(Trypan blue dye exclusion) 20
4.2.4 螢光分析活潑性氧分子生成量 (Fluorescence assay) 21
4.2.5 單細胞凝膠電泳分析(Comet assay) 24
4.2.6核酸醛基損害分析方法 (Aldehyde Reactive Probe-Slot-Blot assay, ASB assay) 27
4.2.7 數據分析 29
第五章實驗結果 30
5.1 空白濾紙細胞毒性測試(MCF-7,BEAS-2B細胞) 30
5.2 QA/QC 30
5.3核酸醛基損害分析方法標準品定量結果 30
5.4 樣本濾紙BEAS-2B細胞實驗 30
5.4.1 細胞毒性測試 31
5.4.2螢光分析活潑性氧分子生成量 31
5.4.3 Comet assay 31
第六章 結果與討論 44
6.1 細胞毒性 44
6.2 活性氧分子 44
6.3 DNA損害 44
第七章未來方向與建議 46
第八章參考文獻 47
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