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研究生:張凱傑
研究生(外文):Kai-Jei Zhang
論文名稱:細懸浮微粒(PM2.5)誘導人類肺支氣管細胞DNA鏈斷裂(BEAS-2B)之研究
論文名稱(外文):Investigation of Fine Airborne Particulate (PM2.5) Induces DNA StrandBreakesin Human Bronchus Cell Lines (BEAS-2B)
指導教授:林伯雄林伯雄引用關係
口試委員:林家驊柯俊良李崇垓
口試日期:2017-06-23
學位類別:碩士
校院名稱:國立中興大學
系所名稱:環境工程學系所
學門:工程學門
學類:環境工程學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:中文
論文頁數:54
中文關鍵詞:細懸浮微粒DNA斷裂
外文關鍵詞:PM2.5DNA break
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在台灣有許多家庭有焚香拜拜的傳統,本次研究使用五種不同來源的線香A、B、C、D、E於實驗室中燃燒,以空氣採樣器收集燃燒線香十分鐘之空氣細懸浮微粒之有機溶液萃取物與水溶液萃取物之混合物對正常人類肺上皮細胞的細胞毒性、氧化壓力與DNA損害之影響。其中A為混和香,B、C、E為沉檀香,D是烏沉香。
在細胞毒性的測試是採用染色計數算存活率的方式,其24小時時毒性由大到小為A、C、D、B、E;在活性氧測試裡30分鐘時氧化壓力的由大到小為B、D、C、E、A,其中A的氧化壓力與負控制組比約1.5倍、B約為2.67倍、C約為2倍、D約2.4倍、E約為1.7倍。結果有所差異的原因可能是線香中的能引發活性氧分子產生的物質作用的速度沒有這麼快,所以短時間內產生的量並不多。
DNA損害是採用彗星試驗進行測定,測試時間為暴露24小時,其DNA損害由大到小為B、A、E、C、D,A與控制組比約14倍,B約為35倍,C約12倍,D約1.35倍,而E約為11.5倍。
There are many families in Taiwan that have a tradition of incense and worship. This study uses five different sources of incense A, B, C, D, and E in the laboratory to burn the air sampler to collect the burning line for ten minutes. Effects of the mixture of organic solution extract and aqueous solution on the cytotoxicity, oxidative stress and DNA damage of normal human lung epithelial cells. Where A is mixed incense, B, C, E for the sink sandalwood, D is black incense.
In the cytotoxicity test is the use of dyeing count the survival rate of the way, the 24 hours when the toxicity from large to small for the A, C, D, B, E; in the active oxygen test 30 minutes when the oxidation pressure from large to small For B, D, C, E, A, where the oxidation pressure of A is about 1.5 times that of negative control group, B is about 2.67 times, C is about 2 times, D is about 2.4 times and E is about 1.7 times. The reason for the difference may be that the amount of substances in the line that can cause the action of the active oxygen molecules is not so fast, so the amount produced in a short time is not much.
DNA damage was measured by comet assay. The test time was 24 hours. The DNA damage was from B to A, E, C, D, A was about 14 times higher than that of control group, B was about 35 times, and C About 12 times, D about 1.35 times, and E is about 11.5 times. 
摘要 i
Abstract ii
縮寫表 iii
目錄 iv
圖目錄 vii
表目錄 ix
第一章 1
1.1 研究緣起 1
1.2研究目的 1
第二章文獻回顧 2
2.1空氣懸浮微粒(PM) 2
2.1.1分類 2
2.1.2成分組成 2
2.2可能吸附之物質與總懸浮微粒 介紹可能吸附在空氣懸浮微粒上的物質和懸浮顆粒有關之實驗研究。 4
2.2.1五氯苯酚(PCP) 4
2.2.2萘醌類衍生物(Nap) 4
2.2.32,3,7,8-四氯二苯並對二噁英(TCDD) 4
2.2.4總懸浮微粒(TSP) 5
2.3空氣懸浮微粒及奈米顆粒對人及生物造成之影響 6
2.3.1細胞毒性 6
2.3.2活性氧分子 7
2.3.3DNA損害 9
2.3.4健康之危害效應 9
2.4 BEAS-2B細胞相關 10
2.5萃取方法 11
第三章實驗設計架構與假設 12
3.1研究假設 12
想了解不同成分的線香樣本燃燒後的PM2.5對細胞毒性、氧化壓力、DNA損害等實驗上能否判斷出何者是最需要被注意的。 12
3.2實驗設計架構 13
第四章實驗材料與方法 14
4.1 實驗設備與材料 14
4.1.1 藥品試劑 14
4.1.2 細胞株 16
4.1.3實驗設備 16
4.2 實驗方法 19
4.2.1細胞培養 19
4.2.2 濾紙萃取 20
4.2.3細胞毒性測試(Trypan blue dye exclusion) 20
4.2.4 螢光分析活潑性氧分子生成量 (Fluorescence assay) 21
4.2.5 單細胞凝膠電泳分析(Comet assay) 24
4.2.6核酸醛基損害分析方法 (Aldehyde Reactive Probe-Slot-Blot assay, ASB assay) 27
4.2.7 數據分析 29
第五章實驗結果 30
5.1 空白濾紙細胞毒性測試(MCF-7,BEAS-2B細胞) 30
5.2 QA/QC 30
5.3核酸醛基損害分析方法標準品定量結果 30
5.4 樣本濾紙BEAS-2B細胞實驗 30
5.4.1 細胞毒性測試 31
5.4.2螢光分析活潑性氧分子生成量 31
5.4.3 Comet assay 31
第六章 結果與討論 44
6.1 細胞毒性 44
6.2 活性氧分子 44
6.3 DNA損害 44
第七章未來方向與建議 46
第八章參考文獻 47
Bai, N., Khazaei, M., van Eeden, S.F. et al. 2007. The Pharmacology of Particulate Matter Air Pollution-Induced Cardiovascular Dysfunction. Pharmacol. Ther. , 113, 16-29.
Bai, Y., Suzuki, A.K., Sagai, M. 2001. The Cytotoxic Effects of Diesel Exhaust Particles on Human Pulmonary Artery Endothelial Cells in Vitro: Role of Active Oxygen Species. Free Radic. Biol. Med. , 30, 555-562.
Bayer-Oglesby, L., Schindler, C., Hazenkamp-von Arx, M.E. et al. 2006. Living Near Main Streets and Respiratory Symptoms in Adults: The Swiss Cohort Study on Air Pollution and Lung Diseases in Adults. Am. J. Epidemiol. , 164, 1190-1198.
Binkova, B., Cerna, M., Pastorkova, A. et al. 2003. Biological Activities of Organic Compounds Adsorbed Onto Ambient Air Particles: Comparison between the Cities of Teplice and Prague during the Summer and Winter Seasons 2000-2001. Mutat. Res. , 525, 43-59.
Bonner, M.R., Han, D., Nie, J. et al. 2005. Breast cancer risk and exposure in early life to polycyclic aromatic hydrocarbons using total suspended particulates as a proxy measure. Cancer Epidemiol Biomarkers Prev. , 14(1), 53-60
Brits, E., Schoeters, G., Verschaeve, L. 2004. Genotoxicity of PM10 and Extracted Organics Collected in an Industrial, Urban and Rural Area in Flanders, Belgium. Environ. Res. , 96, 109-118.
Bronzetti, G., Cini, M., Paoli, M. et al. 1997. Mutagenicity and Chemical Analysis of Airborne Particulate Matter Collected in Pisa. J. Environ. Pathol. Toxicol. Oncol. , 16, 147-156.
Brown, R.K., Wyatt, H., Price, J.F. et al. 1996. Pulmonary Dysfunction in Cystic Fibrosis is Associated with Oxidative Stress. Eur. Respir. J. , 9, 334-339.
Buschini, A., Cassoni, F., Anceschi, E. et al. 2001. Urban Airborne Particulate: Genotoxicity Evaluation of Different Size Fractions by Mutagenesis Tests on Microorganisms and Comet Assay. Chemosphere , 44, 1723-1736.
Calderon-Garciduenas, L., Franco-Lira, M., Torres-Jardon, R. et al. 2007. Pediatric Respiratory and Systemic Effects of Chronic Air Pollution Exposure: Nose, Lung, Heart, and Brain Pathology. Toxicol. Pathol. , 35, 154-162.
Chen, K.S., Lin, C.F., Chou, Y.M. 2001. Determination of Source Contributions to Ambient PM2.5 in Kaohsiung, Taiwan, using a Receptor Model. J. Air Waste Manag. Assoc. , 51, 489-498.
Chiaverini, L. 2002. Asthma, Particulates, and Diesel Exhaust. Med. Health R. I. , 85, 140-142.
Courter, L.A., Musafia-Jeknic, T., Fischer, K. et al. 2007. Urban Dust Particulate Matter Alters PAH-Induced Carcinogenesis by Inhibition of CYP1A1 and CYP1B1. Toxicol. Sci. , 95, 63-73.
Dagher, Z., Garcon, G., Gosset, P. et al. 2005. Pro-Inflammatory Effects of Dunkerque City Air Pollution Particulate Matter 2.5 in Human Epithelial Lung Cells (L132) in Culture. J. Appl. Toxicol. , 25, 166-175.
de Kok, T.M., Driece, H.A., Hogervorst, J.G. et al. 2006. Toxicological Assessment of Ambient and Traffic-Related Particulate Matter: A Review of Recent Studies. Mutat. Res. , 613, 103-122.
de Kok, T.M., Hogervorst, J.G., Briede, J.J. et al. 2005. Genotoxicity and Physicochemical Characteristics of Traffic-Related Ambient Particulate Matter. Environ. Mol. Mutagen. , 46, 71-80.
Delgado-Rodriguez, A., Ortiz-Marttelo, R., Villalobos-Pietrini, R. et al. 1999. Genotoxicity of Organic Extracts of Airborne Particles in Somatic Cells of Drosophila Melanogaster. Chemosphere , 39, 33-43.
Dellinger, B., Pryor, W.A., Cueto, R. et al. 2001. Role of Free Radicals in the Toxicity of Airborne Fine Particulate Matter. Chem. Res. Toxicol. , 14, 1371-1377.
Dockery, D.W., Pope, C.A.,3rd, Xu, X. et al. 1993. An Association between Air Pollution and Mortality in Six U.S. Cities. N. Engl. J. Med. , 329, 1753-1759.
Don Porto Carero, A., Hoet, P.H., Verschaeve, L. et al. 2001. Genotoxic Effects of Carbon Black Particles, Diesel Exhaust Particles, and Urban Air Particulates and their Extracts on a Human Alveolar Epithelial Cell Line (A549) and a Human Monocytic Cell Line (THP-1). Environ. Mol. Mutagen. , 37, 155-163.
Donaldson, K., Brown, D.M., Mitchell, C. et al. 1997. Free Radical Activity of PM10: Iron-Mediated Generation of Hydroxyl Radicals. Environ. Health Perspect. , 105 Suppl 5, 1285-1289.
Ducatti, A., Vargas, V.M. 2003. Mutagenic Activity of Airborne Particulate Matter as an Indicative Measure of Atmospheric Pollution. Mutat. Res. , 540, 67-77.
Fang, G.C., Chang, K.F., Lu, C. et al. 2004. Estimation of PAHs Dry Deposition and BaP Toxic Equivalency Factors (TEFs) Study at Urban, Industry Park and Rural Sampling Sites in Central Taiwan, Taichung. Chemosphere , 55, 787-796.
Fang, G.C., Wu, Y.S., Chang, C.N. et al. 2006. A Study of Polycyclic Aromatic Hydrocarbons Concentrations and Source Identifications by Methods of Diagnostic Ratio and Principal Component Analysis at Taichung Chemical Harbor Near Taiwan Strait. Chemosphere , 64, 1233-1242.
Fang, G.C., Chang, K.F., Lu, C. and Bai, H. 2004. Estimation of PAHs dry deposition and BaP toxic equivalency factors (TEFs) study at Urban, Industry Park and rural sampling sites in central Taiwan, Taichung. Chemosphere, 55:787-796.
Farmer, P.B., Singh, R., Kaur, B. et al. 2003. Molecular Epidemiology Studies of Carcinogenic Environmental Pollutants. Effects of Polycyclic Aromatic Hydrocarbons (PAHs) in Environmental Pollution on Exogenous and Oxidative DNA Damage. Mutat. Res. , 544, 397-402.
Fubini, B., Hubbard, A. 2003. Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS) Generation by Silica in Inflammation and Fibrosis. Free Radic. Biol. Med. , 34, 1507-1516.
Gabelova, A., Valovicova, Z., Horvathova, E. et al. 2004. Genotoxicity of Environmental Air Pollution in Three European Cities: Prague, Kosice and Sofia. Mutat. Res. , 563, 49-59.
Gilli, G., Pignata, C., Schiliro, T. et al. 2007. The Mutagenic Hazards of Environmental PM2.5 in Turin. Environ. Res. , 103, 168-175.
Gilmour, P.S., Brown, D.M., Lindsay, T.G. et al. 1996. Adverse Health Effects of PM10 Particles: Involvement of Iron in Generation of Hydroxyl Radical. Occup. Environ. Med. , 53, 817-822.
Greenwell, L.L., Moreno, T., Jones, T.P. et al. 2002. Particle-Induced Oxidative Damage is Ameliorated by Pulmonary Antioxidants. Free Radic. Biol. Med. , 32, 898-905.
Gutierrez-Castillo, M.E., Roubicek, D.A., Cebrian-Garcia, M.E. et al. 2006. Effect of Chemical Composition on the Induction of DNA Damage by Urban Airborne Particulate Matter. Environ. Mol. Mutagen. , 47, 199-211.
Healey, K., Lingard, J.J., Tomlin, A.S. et al. 2005. Genotoxicity of Size-Fractionated Samples of Urban Particulate Matter. Environ. Mol. Mutagen. , 45, 380-387.
Hetland, R.B., Cassee, F.R., Refsnes, M. et al. 2004. Release of Inflammatory Cytokines, Cell Toxicity and Apoptosis in Epithelial Lung Cells After Exposure to Ambient Air Particles of Different Size Fractions. Toxicol. In. Vitro. , 18, 203-212.
Ho, W.C., Hartley, W.R., Myers, L. et al. 2007. Air Pollution, Weather, and Associated Risk Factors Related to Asthma Prevalence and Attack Rate. Environ. Res.
Hsiao, W.L., Mo, Z.Y., Fang, M. et al. 2000. Cytotoxicity of PM(2.5) and PM(2.5--10) Ambient Air Pollutants Assessed by the MTT and the Comet Assays. Mutat. Res. , 471, 45-55.
Hukkanen, J., Lassila, A., Paivarinta, K. et al. 2000. Induction and Regulation of Xenobiotic-Metabolizing Cytochrome P450s in the Human A549 Lung Adenocarcinoma Cell Line. Am. J. Respir. Cell Mol. Biol. , 22, 360-366.
Karlsson, H.L., Ljungman, A.G., Lindbom, J. et al. 2006. Comparison of Genotoxic and Inflammatory Effects of Particles Generated by Wood Combustion, a Road Simulator and Collected from Street and Subway. Toxicol. Lett. , 165, 203-211.
Karlsson, H.L., Nygren, J., Moller, L. 2004. Genotoxicity of Airborne Particulate Matter: The Role of Cell-Particle Interaction and of Substances with Adduct-Forming and Oxidizing Capacity. Mutat. Res. , 565, 1-10.
Kato, M., Nakano, M., Morikawa, A., Kimura, H., Shigeta, M., Kuroume, T. 1991. Ability of polymorphonuclear leukocytes to generate active oxygen species in children with bronchial asthma. Use of chemiluminescence probes with a Cypridina luciferin analog and luminal. Int. Arch. Allergy Appl. Immunol. 95, 17– 22.
Knaapen, A.M., Shi, T., Borm, P.J. et al. 2002. Soluble Metals as Well as the Insoluble Particle Fraction are Involved in Cellular DNA Damage Induced by Particulate Matter. Mol. Cell. Biochem. , 234-235, 317-326.
Krewski, D., Rainham, D. 2007. Ambient Air Pollution and Population Health: Overview. J. Toxicol. Environ. Health A , 70, 275-283.
Kuo, C.Y., Hsu, Y.W., Lee, H.S. 2003. Study of Human Exposure to Particulate PAHs using Personal Air Samplers. Arch. Environ. Contam. Toxicol. , 44, 454-459.
Li, C.S. and Ro, Y.S. 2000. Indoor characteristics of polycyclic aromatic hydrocarbons in the urban atmosphere of Taipei. Atom. Environ., 34, 611-620.
Li, N., Kim, S., Wang, M., Froines, J., Sioutas, C., Nel, A. 2002. Use of a stratified oxidative stress model to study the biological effects of ambient concentrated and diesel exhaust particulate matter. Inhal. Toxicol. 14, 459–486.
Li, N., Sioutas, C., Cho, A. et al. 2003. Ultrafine Particulate Pollutants Induce Oxidative Stress and Mitochondrial Damage. Environ. Health Perspect. , 111, 455-460.
Lin, T.C., Chang, F.H., Hsieh, J.H. et al. 2002. Characteristics of Polycyclic Aromatic Hydrocarbons and Total Suspended Particulate in Indoor and Outdoor Atmosphere of a Taiwanese Temple. J. Hazard. Mater. , 95, 1-12.
Massolo, L., Muller, A., Tueros, M. et al. 2002. Assessment of Mutagenicity and Toxicity of Different-Size Fractions of Air Particulates from La Plata, Argentina, and Leipzig, Germany. Environ. Toxicol. , 17, 219-231.
Mates, J.M., Perez-Gomez, C., Blanca, M. 2000. Chemical and Biological Activity of Free Radical 'Scavengers' in Allergic Diseases. Clin. Chim. Acta , 296, 1-15.
Matsumoto, H., Adachi, S., Suzuki, Y. 2005. Bisphenol A in Ambient Air Particulates Responsible for the Proliferation of MCF-7 Human Breast Cancer Cells and its Concentration Changes Over 6 Months. Arch. Environ. Contam. Toxicol. , 48, 459-466.
Meng, Z., Zhang, Q. 2007. Damage Effects of Dust Storm PM(2.5) on DNA in Alveolar Macrophages and Lung Cells of Rats. Food Chem. Toxicol.
Muller, A., Alzuet, P., Herbarth, O. et al. 2001. Assessment of Toxicity and Mutagenicity in Air Particulate Matter from an Urban Industrial Area in the Coast of the Rio De La Plata. Environ. Toxicol. , 16, 151-157.
Nielsen, T., Feilberg, A., Binderup, M.L. 1999. The variation of street air levels of PAH and other mutagenic PAC in relation to regulations of traffic emissions and the impact of atmospheric processes. Environ. Sci. Pollut. Res. 6, 133–137.
Palmgren, F., Wahlin, P., Loft, S. 2003. The Pollution of Air with Particles in Copenhagen. Ministry of the Environment, Copenhagen, (in Danish).
Pohjola, S.K., Lappi, M., Honkanen, M. et al. 2003. Comparison of Mutagenicity and Calf Thymus DNA Adducts Formed by the Particulate and Semivolatile Fractions of Vehicle Exhausts. Environ. Mol. Mutagen. , 42, 26-36.
Pozzi, R., Berardis, B.D., Paoletti, L.,and Guastadisegni, C. 2003. Inflammatory mediators induced by coarse (PM 2.5-10) and fine (PM 2.5) urban air particles in RAW 264.7 cells. Toxicology 183, 243-254.
Prahalad, A.K., Inmon, J., Dailey, L.A. et al. 2001. Air Pollution Particles Mediated Oxidative DNA Base Damage in a Cell Free System and in Human Airway Epithelial Cells in Relation to Particulate Metal Content and Bioreactivity. Chem. Res. Toxicol. , 14, 879-887.
Samet, J.M., Dominici, F., Curriero, F.C. et al. 2000. Fine Particulate Air Pollution and Mortality in 20 U.S. Cities, 1987-1994. N. Engl. J. Med. , 343, 1742-1749.
Shi, T., Knaapen, A.M., Begerow, J. et al. 2003. Temporal Variation of Hydroxyl Radical Generation and 8-Hydroxy-2'-Deoxyguanosine Formation by Coarse and Fine Particulate Matter. Occup. Environ. Med. , 60, 315-321.
Sorensen, M., Autrup, H., Moller, P. et al. 2003. Linking Exposure to Environmental Pollutants with Biological Effects. Mutat. Res. , 544, 255-271.
Tong, Y., Ni, X., Zhang, Y. et al. 2002. Study of the Toxicological Mechanism of Acidified Aerosols. Biol. Trace Elem. Res. , 85, 149-156.
Upadhyay, D., Panduri, V., Ghio, A. et al. 2003. Particulate Matter Induces Alveolar Epithelial Cell DNA Damage and Apoptosis: Role of Free Radicals and the Mitochondria. Am. J. Respir. Cell Mol. Biol. , 29, 180-187.
Valavanidis, A., Vlahoyianni, T., Fiotakis, K. 2005. Comparative Study of the Formation of Oxidative Damage Marker 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) Adduct from the Nucleoside 2'-Deoxyguanosine by Transition Metals and Suspensions of Particulate Matter in Relation to Metal Content and Redox Reactivity. Free Radic. Res. , 39, 1071-1081.
Vineis, P., Husgafvel-Pursiainen, K. 2005. Air Pollution and Cancer: Biomarker Studies in Human Populations. Carcinogenesis , 26, 1846-1855.
Zhao, X., Wan, Z., Chen, G. et al. 2002. Genotoxic Activity of Extractable Organic Matter from Urban Airborne Particles in Shanghai, China. Mutat. Res. , 514, 177-192.
Zhao, X.H., Wang, X.L., Li, X.Y. 2001. Automobile Exhaust Particle-Induced Apoptosis and Necrosis in MRC-5 Cells. Toxicol. Lett. , 122, 103-110.
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