臺灣博碩士論文加值系統

茶樹是一種多年生作物，具有在低溫條件下的山區生長之特點，並且很少種植在平原地區。本研究的目的是探討遮蔭對平原地區茶樹生長的影響以及抗氧化能力.本研究選用台茶12號 (金萱) 為試驗材料。種植於台灣台中市中興大學實驗田兩年(2013-2015年)。以遮陰與非遮陰做處理。觀測茶苗去頂後30天和45天抽稍的生長。結果表示，幼苗存活率、株高和抽稍數在陰影中高於非陰影。在（2015年7月）和春季（2016年3月），茶樹去頂後的結果顯示茶樹的株高在陰影中較高，在夏季沒有不同的枝條數和葉片數。春季的結果表示，茶樹株高在無陰影的處理顯著較高，但抽稍數與葉片數沒有顯著差異。在夏季，冬季和春季，遮蔭對切割後茶芽生長的影響是不同的。在DPPH試驗中，遮陰處理顯示出比非遮陰處理有更高的抗氧化能力。因此，可以得出結論，於平原地區，遮蔭處理對於茶苗的生長更好。然而，遮蔭處理對茶苗去頂後生長的影響取決於季節。

Tea plant is one of perennial industry crop that has characteristics of growing in mountain areas with low-temperature condition, and seldom planted in plain area. The purpose of this research was testing the effect of shading on the growth of tea plant in plain area, and on the antioxidant capacity. This research chose cultivar of Taiwan Tea No. 12 as testing materials. Tea was planting at experimental field in National Chung-Hsing University, Taichung, Taiwan for two years (2013-2015).Treatments included shade and non-shade. The growth of tea shoots were monitored after 30 days and 45 days of tea cutting. The results showed that seedling survival rate, plant height and branch number were higher in shade than in non-shade. During summer (July 2015) and spring (March 2016), results of after tea cutting showed that the length of tea shoot was higher in shade significantly and no different in shoot number and leaf number at summer season. The results at spring showed that the length of tea shoot was higher in non-shade significantly and no different in shoot number and leaf number. The effects of shade on the growth of tea shoots after cutting were different between summer, winter and spring season. In DPPH testing shade treatment showed higher antioxidant capacity than non-shade treatment. Therefore, it might be concluded that shade treatment is better for the growth of tea seedling in a plain area. However, the effects of shade on shoot growth after cutting depends on the season.

Acknowledgment i
Abstract iii
List of Figure vii
Chapter 1: Introduction 1
1.1 Background and motivation 1
1.2 Study objectives 1
Chapter 2: Literature reviews 2
2.1 Altitude for the tea growing 2
2.2 The Effect of shade 2
2.3 The Effect of light 3
2.4 Antioxidant activity in tea leaves 4
2.5 Various growing seasons 4
Chapter 3: Materials and methods 7
3.1 Experimental site 7
3.2 Tea leaf collection for antioxidant analysis 7
3.3 Parameters of agronomic traits after 2 years planting at tea field 10
3.3.1 The seedling survival rate (%) 10
3.3.2 The plant height (cm) 10
3.3.3 The branch number (Number) 10
3.4 Parameters of agronomic trait after 30 days and 45 days cutting 10
3.4.1 The length of shoot (cm) 10
3.4.2 Total new leaf number / plant 10
3.4.3 The Shoot number (Number) 11
3.5 Preparation of tea brewing 11
3.6 Making Black Tea 14
3.7 Leaf preparation 14
3.8 Antioxidant capacity by 2, 2 –Diphenyl -1- picrylhydrazyl (DPPH) radical assay
17
3.9 Antioxidant capacity by ferric reducing antioxidant power (FRAP) assay 19
3.10 Tea leaves temperature measurement 19
3.11 Spectrum color of black tea 19
3.12 Statistical Analysis 19
Chapter 4: Results 20
4.1 Result 20
4.1.1 The seedling survival rate (%) 20
4.1.2 The Plant height (cm) 20
4.1.3 The branch number (Number) 20
4.1.4 Tea shoot emerge after cutting in summer season 24
a. The length of shoot (cm) 24
b. Total new leaf/ plant 24
c. The shoot number (Number) 24
4.1.5 Winter season 27
4.1.6 Spring season 28
4.1.7 The leaf temperature 31
4.1.8 Investigation of leaf temperature 31
4.1.9 Spectrum color of black tea 31
4.1.10 Percentage radical scavenging DPPH in fresh tea leaves 36
4.1.11 Percentage radical scavenging DPPH of black tea 36
4.1.12 FRAP Assay 36
Chapter 5: Discussion 40
5.1 Discussion 40
Chapter 6: Conclusion 43
6.1 Conclusion 43
6.2 Further work and suggestion 43
References 44

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