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研究生:江明穎
研究生(外文):Ming-Ying Jiang
論文名稱:探討蝦青素對阿茲海默症大鼠的效用
論文名稱(外文):To Study the Efficacy of Astaxanthin in Alzheimer’s Disease Rat Model
指導教授:陳建榮陳建榮引用關係
指導教授(外文):Jeng-Rung Chen
口試委員:陳儷今王慈娟
口試日期:2017-06-23
學位類別:碩士
校院名稱:國立中興大學
系所名稱:獸醫學系所
學門:獸醫學門
學類:獸醫學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:中文
論文頁數:39
中文關鍵詞:阿茲海默症蝦青素氧化壓力海馬迴樹突棘微小膠細胞
外文關鍵詞:Alzheimer’s diseaseAstaxanthinOxidative stresshippocampusdendritic spinemicroglia
相關次數:
  • 被引用被引用:1
  • 點閱點閱:317
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阿茲海默症是目前最常見的失智症,其早期的症狀表現以記憶退化最為明顯,並隨著病程進展,慢慢會出現語言、空間、計算能力退化。患者的腦部的型態觀察發現神經細胞受到破壞,大腦皮質常可發現Aβ斑塊的堆積。目前有許多理論被提出,近年來腦中氧化壓力的提升被認為可能是阿茲海默症的主因,而蝦青素是一種強抗氧化胡蘿蔔素,且具有長鏈脂肪酸及兩頭親水極性端,能夠穩定的存在細胞膜上,也證實可以通過血腦障壁到達中樞神經系統。因此,我們利用阿茲海默症動物模式探討蝦青素來是否對阿茲海默症有改善的效果。本實驗利用FAB連續四週灌注到側腦室來誘導阿茲海默症大鼠,並在最後一週以腹腔注射給予蝦青素。FAB誘導大鼠在水迷宮的行為檢測發現其空間學習記憶能力變差,組織學的觀察發現FAB誘導後海馬迴CA1錐體細胞凋亡的數量、細胞內Aβ堆積、炎症反應(iNOS、微小膠細胞及星狀膠細胞)均顯著上昇及MS核區內的膽鹼性神經元數量明顯減少,利用細胞內染料注射技術來呈現樹突型態也發現FAB大鼠海馬迴CA1錐體細胞頂樹突的樹突棘密度明顯降低,給予蝦青素處理後明顯讓海馬迴CA1細胞內Aβ堆積、星狀膠細胞數量、膽鹼性神經元的存活數量回復至正常值,也讓動物的空間學習記憶表現獲得回復及部份回復CA1錐體細胞的樹突棘密度至正常狀態。綜合以上的結果顯示蝦青素能降低阿茲海默症大鼠模式下神經細胞損傷、炎症反應、細胞內Aβ堆積及動物行為缺損。
中文摘要 i
Abstract ii
圖表目次 v
前言 - 1 -
材料及方法- 7 -
結果 - 13 -
討論 - 16 -
結論 - 20 -
圖表說明 - 21 -
參考文獻 - 31 -
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