近年來隨著生醫產業的蓬勃發展,無論是在生物檢測、病原體分析、藥物篩檢、化學合成、食品安檢、環境評鑑，微米粒子的分離或排序都佔有重要的一席之地。簡便的分離方式較能順利進行分析和鑑定，而傳統方法的缺點為，儀器設備昂貴、分析時間冗長、分離步驟繁雜等都會增加上述流程的難度。雖然現今已開發出許多分離與聚焦粒子的技術，但相較於其他方式，聲輻射力根據粒子或細胞的大小、密度、壓縮比來進行區分的特性，造成其不需要對細胞預先標記或是表面改質，可以使細胞在新鮮的狀態下進行分離，且適合幾乎所有種類的微粒。同時，利用SSAW裝置來控制粒子或細胞移動到壓力節點或反壓節點，分離和聚焦的過程具有很高的操作性、靈活性和生物相容性。
本研究旨在探討高電壓聚焦所可能產生的邊壁聚焦效應，及相應的解決辦法，當Polystyrene粒子，小球藻(chlorella vulgaris)或是大腸桿菌(E.coli)進行聚焦時，如何完善的將Polystyrene粒子或微藻進行連續式排列至正確的位置。由實驗結果可知，當我們藉由調配溶劑的物理組成，可以處理幾乎所有流道尺寸、粒子濃度、流體流速的邊壁效應問題。

In this study, both preventing aggregation of microparticles near the channel side walls and separation of microparticles by SSAW were investigated. Aggregation of microparticles near the side walls (ie, side wall effect) occurs when using the PDMS based microchannels where the pressure nodes are near the side walls. It was shown that , by using 5:1 glycerol solution as the medium, the acoustic contrast factor became negative, the acoustic microparticles were focused at the pressure anti-nodes, which were away from the channel walls. As to the particle separation, a serpentine channel was placed asymmetrically between the IDTs, such that microparticles with different sizes experienced different acoustic radiant force. The result showed that 5 and 10 um particles can be separated with high separation efficiency.

目錄
中文摘要 I
Extended Abstract II
誌謝 IX
目錄 X
圖目錄 XIII
表目錄 XIX
第一章 緒論 1
1.1 前言 1
1.2 研究動機與方法 2
第二章 文獻回顧 3
2.1 表面聲波 3
2.1.1 表面聲波的源起 3
2.1.2 壓電材料 5
2.1.3 表面駐波聚焦原理 10
2.1.4 表面駐波聚焦的應用 17
2.2聲泳聚焦模型分析 23
2.2.1一維簡諧共振駐波模型 (1D harmonic standing waves model) 23
2.2.2二維聲泳能階聚焦模型(2D SSAW energy potential model) 24
2.2.3 邊壁聚焦效應 29
2.2.4 不同微流道、微流場組合對聲波物理因子的影響 33
2.3 粒子分離技術 36
2.4 微藻 48
第三章 實驗材料與方法 50
3.1 實驗藥品與材料 50
3.2 實驗儀器 56
3.3微藻培養 66
3.3.1藻種與藻種保存 66
3.3.2實驗方法 67
3.4 表面聲波元件之製作 73
3.4.1 指叉狀電極的製作 73
3.4.2 PDMS微流道的製作 78
3.4.3 表面聲波元件的製作 83
3.4.4 表面駐波聚焦測試 84
3.5 螢光強度的檢測 85
3.5.1 Nile red染色法 85
3.5.2 分析與檢測 85
第四章 結果與討論 89
4.1　SSAW聚焦影響參數與邊壁聚焦現象 89
4.2　不同變數於側壁效應的影響 99
4.2.1.1水作為介質 100
4.2.1.2甘油水溶液作為介質 103
4.2.2 微粒子濃度對邊壁聚焦效應的影響 107
4.3　小球藻之聚焦 110
4.4　利用螢光分析法計數待測物粒徑分布 113
4.5　利用蛇行流道(serpentine)設計進行粒子分離 115
第五章 結論 123
第六章 未來工作與建議 124
第七章 參考文獻 125

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