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研究生:吳孟芳
研究生(外文):WU,MENG-FANG
論文名稱:以小鼠模式探討電子菸對於運動能力及血液生化值之影響
論文名稱(外文):Effects of E-Cigarette Exposure on Exercise Performance and Biochemical Profile in Mice
指導教授:黃啟彰黃啟彰引用關係甘乃文甘乃文引用關係
指導教授(外文):HUANG,CHI-CHANGKAN,NAI-WEN
口試委員:黃啟彰甘乃文陳文詮
口試委員(外文):HUANG,CHI-CHANGKAN,NAI-WENCHEN,WEN-CHYUAN
口試日期:2017-06-05
學位類別:碩士
校院名稱:國立體育大學
系所名稱:運動與健康科學學院
學門:民生學門
學類:運動科技學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:中文
論文頁數:46
中文關鍵詞:電子菸尼古丁運動能力肝醣
外文關鍵詞:Electronic cigarettesnicotineexercise performanceglycogen
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目的:探討電子菸 (e-cigarette,EC) 吸入在小鼠中的影響,並檢查EC中不同劑量的尼古丁對運動表現和基本血液生化指數產生的作用。方法:本實驗室透過研究室已建立之小鼠平台,針對電子菸以吸入不同濃度之尼古丁劑量菸液,ICR雌性小鼠隨機分作5組,每組6隻小鼠:正常對照組 (Control),空氣對照組 (Air) ,甘油無尼古丁組 (ELN-0X),1倍電子菸 (ELN-1X),10倍電子菸(ELN-10X),介入14天後,進行前肢抓力、游泳力竭時間以及相關血液生化指標等項目進行分析。以SAS統計軟體進行單因子變異數分析 (one way analysis of variance, ANOVA) ,並以Duncan’s test 測試不同組別處理時是否具有差異,以P<0.05代表具有統計上之意義。結果:實驗數據顯示10倍尼古丁電子菸 (ELN-10X)的前肢抓力分別與其他四組比較起來皆有明顯差異 (p<0.0001)。此外,根據趨勢統計分析 (trend analysis)顯示,前肢抓力的表現也隨著尼古丁劑量倍數增加而有下降趨勢 (p=0.0024)。肝臟和肌肉部位能源儲存量在10倍尼古丁電子菸 (ELN-10X)中,也有顯著差異 (p <0.001),且隨著不同尼古丁劑量倍數吸入趨勢統計分析中呈現明顯下降趨勢。血液相關指標在肝、腎功能,統計分析上並無明顯差異。結論:短期14天暴露於高劑量尼古丁中會影響小鼠肌力及肝醣儲存,在血液生化中肝腎功能及病理組織切片不受影響。
Electronic cigarettes (EC) are becoming a preferred replace for nicotine delivery among many smokers in recent year. In some research support that EC could be useful for smoking cessation. However, EC effects of on human health are inconclusive due to lack of empirical research investigating the presence of EC-induced health hazards or benefits. Thus, we examined the effect of vapor produced by EC on exercise performance and health-related profile in mice model. Female ICR mice were divided into 5 groups (n = 6 per group) for 14 days with no intervention, exposure in air, 0, 0.6mg/mL and 6mg/mL nicotine designated control, Air, ELN-0X, ELN‐1X and ELN‐10X groups, respectively. The results indicated that the EC decreased the grip strength (p = 0.0024) with ELN-10X. EC treatments also produced dose-dependent decreases in liver and muscle glycogen storage (p = 0.0009; p = 0.0003) with ELN-10X. In addition, EC treatment had no negative effect on levels of biochemical indices. We also found the major organ had no adverse effect on morphology. Therefore, we suggest that after use EC could reduce explosive force and glycogen storage capacity. In addition, we could not found any negative effect on biochemical profile and organ morphology.
目 錄
中文摘要
英文摘要
致謝
目錄
表目錄
圖目錄
第壹章 緒論 1
第一節 研究背景與研究動機………………………………………………… 1
第二節 研究目的……………………………………………………………… 4
第貳章 文獻探討 5
第一節 電子菸介紹…………………………………………………………… 5
第二節 香菸與電子菸對健康的影響………………………………………… 9
第三節 尼古丁對運動表現相關研究…………………………………………14
第參章 研究材料與方法 15
第一節 實驗測試樣品………………………………………………………… 15
第二節 實驗動物之飼養與分組……………………………………………… 17
第三節 實驗內容與方法……………………………………………………… 19
第四節 血液與組織樣本之收集及前處理…………………………………… 23
第五節 統計分析……………………………………………………………… 24
第肆章 結果 25
第一節 體重、骨骼肌與代謝器官重量……………………………………… 25
第二節 前肢抓力的表現……………………………………………………… 27
第三節 游泳力竭運動的表現………………………………………………… 28
第四節 電子菸吸入後血液生化值變化情形………………………………… 29
第五節 組織中肝醣含量儲存之效果………………………………………… 31
第六節 體內重要組織病理切片觀察結果…………………………………… 32
第伍章 討論 33
第一節 電子菸吸入不同濃度之尼古丁對於前肢抓力表現影響…………… 33
第二節 電子菸吸入不同濃度之尼古丁對於游泳力竭表現影響…………… 35
第三節 電子菸吸入不同濃度之尼古丁對於血液生化值造成影響………… 36
第四節 電子菸吸入不同濃度之尼古丁對於組織中肝醣儲存之效果……… 38
第陸章 結論 39
參考文獻 40
附錄一 動物實驗審查同意書 46
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