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研究生:韓亞妮
研究生(外文):Maytri Handayani
論文名稱:微生物膠結作用對砂質土壤性質的影響
論文名稱(外文):Effectivity of Biological Cement’s Application to Sandy Soil
指導教授:洪汶宜
指導教授(外文):Emma Yuliani
學位類別:碩士
校院名稱:國立中央大學
系所名稱:土木工程學系
學門:工程學門
學類:土木工程學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:英文
論文頁數:88
中文關鍵詞:生物性膠結接種.
外文關鍵詞:Biological cementInoculation
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本研究目的在瞭解砂土中添加細菌所產生的生物性膠結,探討生物性膠結對砂土的滲透性與剪力強度的改變成度,以期能作為土壤改良的方法。試體準備過程中,將假單胞桿菌注入土壤以產生尿素酶,其中尿素酶轉化為尿素並跟水反應產生碳酸鹽類,引致碳酸鈣在砂質土壤中沉澱。透過碳酸鈣沉澱可以填補砂土之間的縫隙和增加砂土顆粒的鍵結力,提高土壤的剪力強度。試驗中,使用尚未滅菌的土壤在室外進行實驗,其目的在模擬於現地場址中利用生物性膠結改良工法之實際情況,將微生物引致碳酸鈣沉澱方法應用於地盤改良為大地工程領域新的分支,利用定水頭試驗、直接剪力試驗、加州承載比試驗和ESEM (環境掃描電子顯微鏡) 觀測其物理及化學性質變化,經由試驗結果評估該工法於現地施作之可行性。
In this research, the authors tried to analyze the addition of bacterial producer of biological cement on sandy soil to increase its stability. It is expected that the biological cement of bacteria can enhance soil stability by reducing its permeability and increase its shear strength. Pseudomonas sp was injected into the soil to produce urease enzyme, which converts urea that reacted with water become ammonium and carbonate, causing calcite precipitation. Soil stability can be improved by the production of pore-filling materials and particle-binding materials through the calcite precipitation process. This method will be applied to sandy soil by modeling the soil in situ where the soil is non-sterilized, placed outside the room, also exposed to sunlight and air humidity. The aim of these applications is to improve the mechanical properties of soil that it will be more suitable for construction or environmental purposes. This is a new branch of geotechnical engineering that deals with the applications of biological microorganism methods to geological materials used in engineering. After bacterial inoculation, the permeability of sand samples will be tested with constant head test, shear strength with direct shear test, compaction with California Bearing Ratio test, and ESEM (Environmental Scanning Electron Microscopy) test.
摘要 .......................................................................................................................................... I
ABSTRACT ............................................................................................................................ II
ACKNOWLEDGEMENT ................................................................................................... III
TABLE OF CONTENT ...................................................................................................... IV
TABLE CAPTIONS ............................................................................................................ VI
FIGURE CAPTIONS ......................................................................................................... VII
NOTATIONS ........................................................................................................................ IX
CHAPTER 1 INTRODUCTION
1.1 Research Motivation........................................................................................................... 1
1.2 Problems Identification ...................................................................................................... 3
1.3 Research Purposes .............................................................................................................. 4
1.4 Organization Of Thesis ...................................................................................................... 5
CHAPTER 2 LITERATURE REVIEW
2.1 Soil as Resource Material ................................................................................................... 6
2.2 Soil Permeability ................................................................................................................ 7
2.3 Soil Shear Strength ........................................................................................................... 10
2.4 California Bearing Ratio Test .......................................................................................... 14
2.5 Scanning Electron Microscope Test ................................................................................. 16
2.6 Bacterial Can Be Used for Biological Cement‘s Application into The Soil..................... 17
2.6.1 Pseudomonas sp. .................................................................................................... 18
2.7 Growth and Development of Bacteria Inside The Soil .................................................... 19
2.8 Taiwan Zhongli District Temperature and Air Humidity ................................................ 20
2.9 Recapitulation of Relative Study .................................................................................... 22
CHAPTER 3 RESEARCH METHODOLOGY
3.1 Primary Data Collection Technique ................................................................................. 24
3.2 Research Location ............................................................................................................ 24
V
3.3 Research Material and Equipment ................................................................................... 25
3.3.1 Material .................................................................................................................. 26
3.3.2 Equipment .............................................................................................................. 29
3.4 Research Stages ................................................................................................................ 31
3.5 Treatments and Conditions of Sample ............................................................................. 32
3.6 Relative Density of Soil .................................................................................................... 34
3.7 Material (Sample) Preparation ......................................................................................... 37
3.7.1 Direct Shear Mould and Constant Head Mould Sample Preparation .................... 37
3.7.2 California Bearing Ratio Mould Sample Preparation ............................................ 39
3.7.3 Scanning Electron Microscope Test Sample Preparation ...................................... 40
3.8 Inoculation Bacteria .......................................................................................................... 41
3.9 Environment Conditions for Bacterial Growth and Development ................................... 45
3.10 Experiment‘s Temperature And Air Humidity ............................................................. 46
CHAPTER 4 TEST RESULTS AND DISCUSSIONS
4.1 Effect of Inoculation on Sample Structure ........................................................................ 49
4.2 Constant Head Permeability Test Results ......................................................................... 52
4.3 Direct Shear Test Results .................................................................................................. 56
4.4 California Bearing Ratio Test Result ............................................................................... 59
4.5 Scanning Electron Microscope Test Result ..................................................................... 63
4.6 Comparison With Previous Research in Indonesia .......................................................... 65
4.7 Limitation of Biological Cement‘s Application into The Soil ......................................... 66
CHAPTER 5 CONCLUSIONS AND RECOMMENDATIONS
5.1 Conclusions ....................................................................................................................... 68
5.2 Recommendations And Suggestions ................................................................................ 69
REFERENCES ..................................................................................................................... 71
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