臺灣博碩士論文加值系統

本論文利用反摺積技術重建R6G螢光分子在金屬奈米結構上隨機發光的過程，以獲得一張超解析影像。透過金屬電漿子增強螢光訊號強度，來凸顯金屬與基板訊號上的差異，再控制螢光分子濃度使之稀疏發光，進而達到單分子發光，由於布朗運動的關係，螢光分子會隨機出現在樣品上，錄製每個時刻的螢光影像，再透過反摺積運算，疊加一張張反摺積後的螢光影像，便能描繪出金屬奈米結構。若能成功達到與電子顯微鏡相同解析度，便是建立了一項超解析影像技術，此技術將超越光學顯微鏡的解析度，且不具有像電子顯微鏡有破壞樣品的風險。

This research by using the method of deconvolution rebuild the process of random blinking caused by Rhodamine 6G on nanoscale metal structures, obtaining a super resolution image as result. In order to enhance the S/N ratio between nanoscale metal structures and substrates, we take advantage of surface plasmons to rise the intensity of fluorescence on nanoscale metal structures. Further, by adjusting the concentration of Rhodamine 6G solution to achieve the goal of single molecular blinking.
By Brownian motion theory, Rhodamine 6G molecules would appear on sample randomly. With the films and images of fluorescence taking place on nanoscale metal structures, we could rebuild the morphology of nanoscale metal structures through deconvolution calculations. If the resolution of the final result reach the same level as SEM, it can be seen as a super resolution technique. This technique could provide higher resolution images than conventional optical microscopes, also could avoid the risk of destroying samples by using SEM.

中文摘要 i
Abstract ii
誌謝 iii
目錄 iv
圖目錄 vi
表目錄 viii
第一章 緒論 1
1.1前言 1
1.2研究背景 3
 近場掃描光學顯微術 4
 共焦掃描顯微術 5
 受激放射消去顯微術 6
 單分子定位顯微術 8
 結構化照明顯微術 8
1.3研究動機與目的 11
1.4論文架構概述 12
第二章 原理與研究方法 13
2.1 螢光發光原理 13
2.1.1 Jablonski 能階圖 13
2.1.2 生命週期、量子產率 16
2.1.3 螢光特性介紹 18
2.2 金屬與螢光分子之交互作用 19
2.2.1金屬物質特性 19
2.2.2表面增強效應 27
2.2.3 金屬與螢光分子間的增強與猝滅 33
2.3 單分子定位 36
2.3.1 Localization-based－質心座標定位 37
2.3.2 Image-deconvolution-based 41
第三章 樣品製作與實驗架構 47
3.1實驗流程 47
3.2樣品製作 48
3.3螢光量測 50
3.4 單分子中心定位 53
第四章 實驗結果與討論 59
4.1遠場量測結果 59
4.2重建超解析影像結果與分析 64
第五章 結論與未來展望 68
參考文獻 69

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