跳到主要內容

臺灣博碩士論文加值系統

(18.97.9.175) 您好!臺灣時間:2024/12/07 22:22
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:李宗穎
研究生(外文):Zong-Ying Li
論文名稱:應用抗凍輔助劑促進珊瑚卵母細胞低溫 保存能力之研究
論文名稱(外文):Investigation of cryoprotective supplements on low temperature-preserved coral oocytes
指導教授:林家興林家興引用關係
指導教授(外文):Chia-Hsin Lin
學位類別:碩士
校院名稱:國立東華大學
系所名稱:海洋生物研究所
學門:自然科學學門
學類:海洋科學學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
論文頁數:54
中文關鍵詞:珊瑚卵母細胞低溫冷凍保存冷凍保護劑超微結構
相關次數:
  • 被引用被引用:0
  • 點閱點閱:345
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
全球珊瑚礁遭受嚴重威脅,過去30 年,世界各地珊瑚礁對於溫暖的熱擾動已
成為司空見慣。而這些變動會導致居住於珊瑚組織內部的共生藻之共生關係造成
崩潰,迫使共生藻離開宿主,最終珊瑚出現白化並面臨死亡的局面。低溫冷凍保
存技術,主要是利用低溫將細胞、組織、胚胎的代謝活動趨近於停滯狀態,可有
效地保存遺傳物質。超微結構分析技術,能使我們更加精確去了解與分析細胞內
部結構。本次實驗利用低溫儀進行(5°C 和-5°C)低溫實驗探討低溫下,不同濃度的
抗凍劑對於石珊瑚小芽棘孔珊瑚(Echinopora gemmacea)與柳珊瑚紅鞭珊瑚
(Junceella juncea)卵母細胞在低溫存下和暴露時間的不同所造成的影響。利用穿透
式電子顯微鏡探討多孔表孔珊瑚(Montipora millepora)、青灰表孔珊瑚(Montipora
grisea),珊瑚卵母細胞其結構。探討低溫對於卵母細胞添加同濃度抗凍劑之影響。
總體而言,添加了抗凍劑(葡萄醣,蔗醣和海藻醣)在5°C 低溫實驗中,能提高珊瑚
卵母細胞在低溫下存活率和耐受性,而蔗醣與甲醇的組合為本次實驗的最佳抗凍
劑。而J. juncea 相較於E. gemmacea 對於低溫更具有耐受性。多孔表孔珊瑚、青
灰表孔珊瑚石珊瑚卵母細胞超微結構分析,觀察內部胞器結構與分布,微絨毛能
提供卵母細胞保護機制,提高精子附著機率,卵黃體與脂質體可作為能量儲備之
胞器,且多孔表孔珊瑚、青灰表孔珊瑚內部皆觀察到共生渦邊毛藻,共生藻能提
供養分並使脂質體產生型態的改變。本次研究可應用於其他珊瑚物種之卵母細胞
低溫保存與比較不用種珊瑚的結構差異性,並提升爾後低溫保存技術之研究與發
展。
In the past 30 years, global warming caused serious damage towards the coral
species which affected the mutualism relationship between algae and coral and caused
algae to leave its coral host and consequently, coral bleaching. cryopreservation is a
technique that use low temperature to stop the metabolic activity of cell, tissues and
embryos in order to preserve the genetic material effectively. The used of transmission
electron microscopy (TEM) technique help to analyze and study internal ultrastructure
of the cell more accurately. In this experiment, the effect of low temperature cooling and
the exposure time toward Echinopora gemmacea and Junceella juncea oocytes with a
variety of cryoprotectant were studied by using dry bath (5°C and -5°C). The effect of
different concentration of cryoprotectants towards Montipora millepora and Montipora
grisea oocytes structure were studied by using TEM. Based on the research, the used of
cryprotectants (glucose, sucrose and trehalose) improved the survival rate of the coral
oocytes under 5°C cooling, whereas combination of sucrose with methanol proved to be
the most efficient cryoprotectant. Besides, J. juncea showed better cold tolerance
compare to both E. gemmacea. Based on the TEM observation of the coral internal
ultrastructure, it showed that micovilli provided protection towards coral oocytes and
improve the attachment of sperms towards oocytes while yolk body and lipid granules
acted as source of energy for coral oocytes. Meanwhile, algae not only provided energy
to the coral, it can also change the structure of lipid granules. This research can be
conducted on different species of coral oocytes to improve and develop future
cryopreservation method.
摘要 I
Abstract II
目錄 III
圖目錄 V
表目錄 VI
第一章 前言 1
1.1 冷凍保存技術 2
1.2 影響冷凍保存之因子 3
1.2.1 冷凍條件之影響 3
1.2.2 細胞滲透壓 3
1.2.3 冰晶的形成 4
1.3 冷凍保護劑 4
1.3.1 穿透性抗凍劑( Permeating cryoprotectant) 5
1.3.2 非穿透性抗凍劑(Non-permeating cryoprotectant) 5
1.4 醣類 7
1.4.1 醣類功能 7
1.5 低溫傷害 10
1.6 低溫冷凍保存之應用 10
1.6.1 低溫生物學之應用 10
1.6.2 微生物與生物工程 10
1.6.3 農牧和水產養殖 11
1.7 超微結構分析 11
1.8 低溫冷凍保存之超微結構分析應用 12
1.8.1 魚類配子低溫冷凍保存之超微結構之分析 13
1.8.1.1 精子 13
1.8.1.2 胚胎 13
1.8.1.3 卵母細胞 15
1.8.2 無脊椎動物低溫冷凍保存之超微結構之分析 15
1.8.3 珊瑚低溫冷凍保存超微結構之分析 17
第二章 材料與方法 19
2.1 採集地點 19
2.2 低溫下抗凍劑對於卵母細胞之影響 20
2.2.1 ATP測定 20
2.2.2 統計分析 20
2.3 穿透式電子顯微鏡Transmission ElectronMicroscopy:TEM 21
第三章 珊瑚卵母細胞低溫保存之比較 23
3.1 前言 23
3.2 結果 24
3.3 討論 29
3.4 結論 31
第四章 珊瑚卵母細胞之超微結構 33
4.1 前言 33
4.2 結果 34
4.2.1 M. grisea卵母細胞超微結構 35
4.2.2 M. millepora卵母細胞超微結構 36
4.3 卵母細胞之胞器統計結果 53
4.4 討論 54
4.5 結論 57
參考文獻 59
附錄一 71
附錄二 73
Adams, SL., Smith, JF., Roberts, RD., Janke, AR., Kaspar, HF., Tervit, HR., Pugh, PA.,Webb, SC., King, NG (2004) Cryopreservation of sperm of the Pacific oyster (Crassostrea gigas): development of a practical method for commercial spat production. Aquaculture. 242(1-4), 271-282.
Aeby, GS., Callahan, S., Cox, EF., Runyon, C., Smith, A., Stanton, FG., Work, TM (2016) Emerging coral diseases in Kāne ‘ohe Bay, O ‘ahu, Hawai ‘i (USA): two major disease outbreaks of acute Montipora white syndrome. Diseases of aquatic organisms. 119(3), 189-198.
Afzelius, BA (1978) Fine structure of the garfish spermatozoon. Journal of ultrastructure research. 64(3), 309-314.
Allison, S., Chang, B., Randolph, T., Carpenter, J (1999) Hydrogen bonding between sugar and protein is responsible for inhibition of dehydration-induced protein unfolding. Archives of Biochemistry and Biophysics. 365(2), 289-298.
Amann, RP (1999) Cryopreservation of sperm. In: Knobil, E., Neill, J.D. (Eds.), Encyclopedia of Reproduction. Academic Press, Burlington, MA, pp. 773-783.
Anchordoguy, TJ., Rudolph, AS., Carpenter, JF., Crowe, JH (1987) Modes of interaction of cryoprotectants with membrane phospholipids during freezing. Cryobiology. 24, 324-331.
Aramli, MS., Golshahi, K., Nazari, RM., Aramli, S., Banan, A (2015) Effectiveness of glucose-methanol extender for cryopreservation of Huso huso spermatozoa. Animal Reproduction Science. 162, 37-42.
Asahina, E., Takahashi, T (1978) Freezing tolerance in embryos and spermatozoa of the sea urchin. Cryobiology. 15(1), 122-127.
Asahina, E., Takahashi, T (1979) Cryopreservation of sea urchin embryos and sperm. Development, Growth & Differentiation. 21(5), 423-430.
Azevedo, C., Castilho, F., Coimbra, A (1984) Fine structure and cytochemistry of the oocyte nucleolus in the mollusk Helcion pellucidus (Prosobranchia). Journal of ultrastructure research. 89(1), 1-11.
Bellas, J., Paredes, E (2011) Advances in the cryopreservation of sea–urchin embryos: potential application in marine water quality assessment. Cryobiology. 62,174-180.
Benson, EE (2008) Cryopreservation theory. In: Reed, B.M. (ed) Plant Cryopreservation. A Practical Guide. Springer. New York. 15-32. Benson, SM., Cole, DR (2008) CO2 sequestration in deep sedimentary formations. Elements. 4(5), 325-331.
Blades‐Eckelbarger, PI., Youngbluth, MJ (1984) The ultrastructure of oogenesis and yolk formation in Labidocera aestiva (Copepoda: Calanoida). Journal of Morphology. 179(1), 33-46.
Blaxter, JHS (1953) Sperm storage and cross-fertilization of spring and autumn spawning herring. Nature. 172, 1189-1190.
Brown, BE (1990) Damage and recovery of coral reefs affected by El Niño related seawater warming in the Thousand Islands, Indonesia. Coral reefs. 8(4), 163-170.
Burns, JH., Takabayashi, M (2011) Histopathology of growth anomaly affecting the coral, Montipora capitata: implications on biological functions and population viability. PloS ONE. 6(12), e28854.
Calcott, PH (1978) Freezing and thawing microbes. Meadowfield Press, ISA Building.
Cárdenas, R., Chávez, M., González, JL., Aley, P., Espinosa, J., Jiménez-García, LF (2008) Oocyte structure and ultrastructure in the Mexican silverside fish Chirostoma humboldtianum(Atheriniformes: Atherinopsidae). Revista de biología tropical. 56(4), 1825-1835.
Chao, NH., Lin, TT., Chen, YJ., Hsua, H. W., Liao, IC (1997) Cryopreservation of late embryos and early larvae in the oyster and hard clam. Aquaculture. 155(1-4), 31-44.
Choi, YH., Chang, YJ (2003) The influence of cooling rate, developmental stage, and the addition of sugar on the cryopreservation of larvae of the pearl oyster Pinctada fucata martensii. Cryobiology. 46, 190-193.
Choi, YH., Chang, YJ (2014) Influences of developmental stages, protective additives and concentrations of cryoprotective agents on the cryopreservation of pacific oyster (Crassostrea gigas) larvae. CryoLetters. 35, 495-500.
Choi, YH., Lee, JY., Chang, YJ (2008) The influence of developmental stages and protective additives on cryopreservation of surf clam (Spisula sachalinensis) larvae. Journal of Environmental Biology. 29(4), 461-463.
Ciereszko, A., Dietrich, GJ., Nynca, J., Dobosz, S., Zalewski, T (2014) Cryopreservation of rainbow trout semen using a glucose-methanol extender. Aquaculture. 275-281.
Corteel, JM (1974) Viability of goat spermatozoa deep frozen with or without seminal plasma: glucose effect. Annales de Biologie Animale, Biochimie, Biophysique. 14, 741-745.
Crain, CM., Halpern, BS., Beck, MW., Kappel, CV (2009) Understanding and managing human threats to the coastal marine environment. Annals of the New York Academy of Sciences. 1162(1), 39-62.
Crowe, JH., Carpenter, JF., Crowe, LM., Anchordoguy, TJ (1990) Are freezing and dehydration similar stress vectors? A comparison of modes of interaction of stabilizing solutes with biomolecules. Cryobiology. 27, 219-231.
Crowe, LM., Crowe, JH., Chapman, D (1985a) Interaction of carbohydrates with dry dipalmitoylphosphatidylcholine. Archives of Biochemistry and Biophysics. 236, 289-296.
Crowe, LM., Crowe, JH., Rudolph, A., Womersley, C., Appel, L (1985b) Preservation of freeze-dried liposomes by trehalose. Archives of Biochemistry and Biophysics. 242(1), 240-247.
Dinnyes, A., Urbányi, B., Baranyai, B., Magyary, I. (1998) Chilling sensitivity of carp (Cyprinus carpio) embryos at different developmental stages in the presence or absence of cryoprotectants: Work in progress. Theriogenology. 50(1), 1-13.
Donner, SD (2011) An evaluation of the effect of recent temperature variability on the prediction of coral bleaching events. Ecological Applications. 21(5), 1718-1730 DOI 10.1890/10-0107.1.
Eckelbarger, KJ., Davis, CV (1996) Ultrastructure of the gonad and gametogenesis in the eastern oyster, Crassostrea virginica. I. Ovary and oogenesis. Marine Biology. 127(1), 79-87.
Eddy, EM., Shapiro, BM (1976) Changes in the topography of the sea urchin egg after fertilization. The Journal of Cell Biology. 71(3), 5-48.
Eghbert, EA., Johan, O., Menkes, CE., Niño, F., Birol, F., Ouillon, S., Andréfouët, S (2017) Coral mortality induced by the 2015-2016 El-Niño in Indonesia: the effect of rapid sea level fall. Biogeosciences. 14(4), 817.
Eroğlu, A (2010) Cryopreservation of mammalian oocytes by using sugars: Intra- and extracellular raffinose with small amounts of dimethylsulfoxide yields high cryosurvival, fertilization, and development rates. Cryobiology. 60(3S), S54-S59.
Eroğlu, A., Bailey, SE., Toner, M., Toth, TL (2009) Successful cryopreservation of mouse oocytes by using low concentrations of trehalose and dimethylsulfoxide. Biology of Reproduction. 80, 70-78.
Fernandez-Santos, MR., Martınez-Pastor, F., Garcıa-Macıas, V., Esteso, MC., Soler, AJ., de Paz, P., Anel, L., Garde, JJ (2007) Extender osmolality and sugar supplementation exert a complex effect on the cryopreservation of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa. Theriogenology. 67, 738-753.
Gaino, E., Scoccia, F (2008) Female gametes of the black coral Cirrhipathes cfr. anguina (Anthozoa, Antipatharia) from the Indonesian Marine Park of Bunaken. Invertebrate Reproduction & Development. 51(3), 119-126.
Gaino, E., Scoccia, F (2009) Release of sperm clusters in spheres by the black coral Cupressopathes pumila (Anthozoa, Antipatharia). Coral reefs. 28(4), 851-857.
Gest, H (2004) The discovery of microorganisms by Robert Hooke and Antoni Van Leeuwenhoek, fellows of the Royal Society. Notes and Records of the Royal Society. 58(2), 187-201.
Griffiths, JB., Cox, CS., Beadle, DJ., Hunt, CJ., Reid, DS (1979) Changes in cell size during the cooling, warming and post-thawing periods of the freeze-thaw cycle. Cryobiology. 16(2), 141-151.
Gutiérrez, J., Jones CG (2008) Ecosystem engineers. In Encyclopedia of Life Sciences. John Wiley & Sons: Chichester.
Gwo, JC (2000) Cryopreservation of sperm of some fishes. In:Tiersch TR, Mazik PM (eds) Cryopreservation in Aquatic Species,pp. 138-160. World Aquaculture Society, Baton Rouge, LA.
Hagedorn, M. Carter, VL (2015) Seasonal preservation success of the marine dinoflagellate coral symbiont, Symbiodinium sp. PLoS ONE. 10(9), e0136358.
Hagedorn, M., Carter, V., Martorana, K., Paresa, MK., Acker, J (2012) Preserving and using germplasm and dissociated embryonic cells for conserving Caribbean and Pacific coral. PLoS ONE.7(3), e33354.
Hagedorn, M., Carter, VL., Leong, JC., Kleinhans, FW (2010) Physiology and cryosensitivity of coral endosymbiotic algae (Symbiodinium). Cryobiology. 60(2), 147-58.
Hagedorn, M., Farrell, A., Carter, VL (2013) Cryobiology of coral fragments. Cryobiology. 66(1), 17-23.
Hagedorn, M., Lance, SL., Fonseca, DM., Kleinhans, FW., Artimov, D., Fleischer, R., Hoque, ATMS., Hamilton, MB.., Pukazhenthi, BS (2002) Altering Fish Embryos with Aquaporin-3: An Essential Step Toward Successful Cryopreservation. Biology of reproduction. 67(3), 961-966.
Hagedorn, M., Pan, R., Cox, EF., Hollingsworth, L., Krupp, D., Lewis, TD., Leong, JC., Mazur, P., Rall, WF., MacFarlane, DR., Fahy, G., Kleinhans, FW (2006) Coral larvae conservation: Physiology and reproduction. Cryobiology. 52, 33-47.
Hamaratoglu, F., Roglu, A., Toner, M., Sadler, KC (2005) Cryopreservation of starfish oocytes. Cryobiology. 50, 38-47.
He S., Woods, LC (2004) Changes in motility, ultrastructure, and fertilization capacity of striped bass Morone saxatilis spermatozoa following cryopreservation. Aquaculture. 236(1), 677-686.
He, S., Woods, LC (2004). Effects of dimethyl sulfoxide and glycine on cryopreservation induced damage of plasma membranes and mitochondria to striped bass (Morone saxatilis) sperm. Cryobiology. 48(3), 254-262.
Hincha, DK., Popova, AV., Cacela, C. (2006) Effects of sugars on the stability and structure of lipid membranes during drying. In: Leitmannova A, editor. Advances in planar lipid bilayers and liposomes. Academic Press. pp. 127-89.
Hinsch, GW., Cone, MV (1969) Ultrastructural observations of vitellogenesis in the spider crab, Libinia emarginata L. The Journal of cell biology. 40(2), 336-342.
Hisaoka, KK., Battle, HI (1958) The normal developmental stages of the zebrafish, brachydanio rerio (hamilton‐buchanan). Journal of Morphology. 102(2), 311-327.
Horváth, A., Miskolczi, E., Urbányi, B (2003) Cryopreservation of common carp sperm. Aquatic Living Resources, 16(5), 457-460.
Isachenko, E., Isachenko, V., Weiss, JM., Kreienberg, R., Katkov, II., Schulz, M., Lula, AGMI., Risopatron, MJ., Sanchez, R (2008) Acrosomal status and mitochondrial activity of human spermatozoa vitrified with sucrose. Reproduction. 136(2), 167-173.
Jafaroghli, M., Khalili, B., Farshad, A., Zamiri, MJ (2011) The effect of supplementation of cryopreservation diluents with sugars on the post-thawing fertility of ram semen, Small Ruminant Research. 96, 58-63.
Kato, KH., Washitani‐Nemoto, S., Hino, A., Nemoto, SI (1990) Ultrastructural studies on the behavior of centrioles during meiosis of starfish oocytes. Development, growth & differentiation. 32(1), 41-49.
Kawaroe, M., Soedharma, D (2007) Oogenesis Karang Sclerectinia Caulastrea furcata dan Lobophyllia corymbosa. Hayati Journal of Biosciences. 14(1), 31-35.
Kim, KT., Lim, HK., Chang, YJ (2013) Survival rates with time course of frozen–thawed pacific oyster larvae in indoor rearing system. Development & Reproduction. 17, 337-343.
Kirsop, BE., Doyle, A (1991) Maintenance of microorganisms and cultured cells (pp. 17-18). London: Academic Press.
Koseoglu, M., Eroglu, A., Toner, M., Sadler, KC (2001) Starfish oocytes form intracellular ice at unusually high temperatures. Cryobiology. 43, 248-259.
Koster, KL., Lei YP., Anderson, M., Martin, S., Bryant, G (2000) Effects of vitrified and non–vitrified sugars on phosphatidylcholine fluid–to–gel phase transitions. Biophysical Journal. 78,1932-1946.
Kudo, M., Kameda, J., Saruwatari, K., Ozaki, N., Okano, K., Nagasawa, H., Kogure, T (2010) Microtexture of larval shell of oyster, Crassostrea nippona: A FIB-TEM study. Journal of structural biology. 169(1), 1-5.
Lam, KK (2000) Sexual reproduction of a low-temperature tolerant coral Oulastrea crispata (Scleractinia, Faviidae) in Hong Kong, China. Marine Ecology Progress Series. 205, 101-111.
Leekumjorn, S., Sum, AK (2008) Molecular dynamics study on the stabilization of dehydrated lipid bilayers with glucose and trehalose. The Journal of Physical Chemistry. 112, 10732-10740.
Levine, H., Slade, L (1992) Another view of trehalose for drying and stabilizing biological materials. BioPharm. 5, 36-40.
Levitt, J (1980) Responses of Plants to Environmental Stress, Volume 1: Chilling, Freezing, and High Temperature Stresses. Academic Press.
Lin, C., Tsai, S (2012) The effect of chilling and cryoprotectants on hard coral (Echinopora spp.) oocytes during short-term low temperature preservation. Theriogenology. 77(6), 1257-1261.
Lin, C., Zhang, T., Kuo, F. W., Tsai, S (2011) Gorgonian coral (Junceella juncea and Junceella fragilis) oocyte chilling sensitivity in the context of adenosine triphosphate response (ATP). Cryoletters. 32(2), 141-148.
Lin., C., Wang, LH., Meng, PJ., Chen, CS., Tsai, S (2013) Lipid content and composition of oocytes from five coral species: potential implications for future cryopreservation efforts. PLoS ONE. 8(2), e57823.
Liu, QH., Li, J., Zhang, SC., Xiao, ZZ., Ding, FH., Yu, DD., Xu, XZ (2007) Flow cytometry and ultrastructure of cryopreserved red seabream (Pagrus major) sperm. Theriogenology. 67(6), 1168-1174.
Liu, Y., Xu, T., Robinson, N., Qin, J., Li, X (2014) Cryopreservation of sperm in farmed blacklip abalone ( Haliotis rubra Leach, 1814). Aquaculture Research. 1-9.
Liu, YB., Xu, T., Robinson, N., Qin, JG., Li, XX (2014) Cryopreservation of sperm in farmed Australian greenlip abalone Haliotis laevigata. Cryobiology. 68, 185-193.
Liu, YB., Xu, T., Robinson, N., Qin, JG., Li, XX (2015) Cryopreservation of sperm in farmed blacklip abalone (Haliotis rubra Leach, 1814). Aquaculture Research. 46, 2628-2636.
Lough, J (2007) Chapter 2: Climate and Climate Change on the Great Barrier Reef. In Climate Change and the Great Barrier Reef, eds. Johnson JE and Marshall PA. Great Barrier Reef Marine Park Authoritycand Australian Greenhouse Office, Australia. pp. 13.
Maisse, G (1996) Cryopreservation of fish semen: a review. Proceedings of the refrigeration science and technology conference, refrigeration and aquaculture. Institut International du Froid, Paris, France, pp. 443-467.
Mazur, P (1984) Freezing of living cells: mechanisms and implications. American Journal of Physiology Cell Physiology. 247(3), C125-C142.
McClanahan, T., Polunin, N., Done, T (2002) Ecological states and the resilience of coral reefs. Conservation ecology. 6(2), 18.
Meryman, HT (2007) Cryopreservation of living cells: principles and practice. Transfusion. 47(5), 935-945.
Meryman, HT., Williams, RJ., Douglas, MSJ (1977) Freezing injury from “solution effects” and its prevention by natural or artificial cryoprotection. Cryobiology. 14(3), 287-302.
Moran, AL., McAlister, JS (2009) Egg size as a life history character of marine invertebrates: is it all it's cracked up to be?. The Biological Bulletin. 216(3), 226-242.
Morris, GJ. Watson, PF (1984) Cold shock injury - a comprehensive bibliography. CryoLetters. 5, 352-372.
Morris.GJ., Clarke,A (1987) Cells at low temperatures. In Grout,B.W.W. and Morris.GJ. (eds), The Effects of Low Temperature on Biological Systems. Edward Arnold, London, pp. 72-119.
Muldrew, K., McGann, LE (1994) The osmotic rupture hypothesis of intracellular freezing injury. Biophysical Journal. 66(2), 532-541.
Naing, SW., Wahid, H., Azam, KM., Rosnina, Y., Zuki, AB., Kazhal, S., Bukar, MM., Thein, M., Kyaw, T., San, MM (2010) Effect of sugars on characteristics of Boer goat semen after cryopreservation, Animal Reproduction Science. 122, 23-28.
Najmudeen, TM (2008) Ultrastructural studies of oogenesis in the variable abalone Haliotis varia (Vetigastropoda: Haliotidae). Aquatic Biology. 2, 143-151.
Ninhaus Silveira, A., Foresti, F., de Azevedo, A., Agostinho, CÂ., Veríssimo-Silveira, R (2009) Cryogenic preservation of embryos of Prochilodus lineatus (Valenciennes, 1836)(Characiforme; Prochilodontidae). Zygote. 17(01), 45-55.
Obura, DO (2005) Resilience and climate change: lessons from coral reefs and bleaching in the Western Indian Ocean. Estuarine Coastal and Shelf Science. 63(3), 353-372.
Odintsova, NA., Ageenko, NV., Kipryushina, YO., Maiorova, MA., Boroda, AV (2015) Freezing tolerance of sea urchin embryoniccells: differentiation commitment and cytoskeletal disturbancesin culture. Cryobiology. 71(1), 54-63.
Ohki, S., Morita, M., Kitanobo, S., Kowalska, AA., Kowalski, RK (2014) Cryopreservation of Acropora digitifera sperm with use of sucrose and methanol based solution. Cryobiology. 69, 134-139.
Oliveira- Almeida, IR., Buzollo, H., da Silva Costa, R., Veríssimo Silveira, R., Porto Foresti, F., Ninhaus Silveira, A (2015). Structural analysis of embryogenesis of Leiarius marmoratus (Siluriformes: Pimelodidae). Zygote. 23(05), 742-757.
Padilla-Gamiño, JL., Hédouin, L., Waller, RG., Smith, D., Truong, W., Gates, RD (2014) Sedimentation and the reproductive biology of the Hawaiian reef-building coral Montipora capitata. The Biological Bulletin. 226(1), 8-18.
Padilla-Gamiño, JL., Weatherby, TM., Waller, RG., Gates, RD. (2011) Formation and structural organization of the egg-sperm bundle of the scleractinian coral Montipora capitata. Coral Reefs. 30(2), 371-380.
Pakes, MJ., Woollacott, RM (2008) Reproduction of the gorgonian Plexaura flexuosa in Bermuda. Journal of Experimental Marine Biology and Ecology. 357(2), 121-127.
Pearl, M., Arav, A (1999) Chilling sensitivity in zebrafish (Brachydanio rerio) oocytes is related to lipid phase transition. CryoLetters. 21(3), 171-178.
Pegg, DE (2007) Principles of cryopreservation. Cryopreservation and freeze-drying protocols. 39-57.
Pereira, RM., Marques, CC (2008) Animal oocyte and embryo cryopreservation. Cell and tissue banking. 9(4), 267-277.
Polge, C., Smith, AU., Parkes, AS (1949) Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature. 164, 666.
Quinn, PJ (1985) A lipid–phase separation model of low–temperature damage to biological membranes. Cryobiology. 22(2), 128-146.
Radhakrishna, TG., Ramanatha, CL., Gupta, C., Reddy, NC (1983) Economics of artificial insemination and calves born from liquid semen and frozen semen. The Indian Journal of Animal Reproduction. 3, 44-45.
Rall, WF., Mazur, PETER., McGrath, JJ (1983) Depression of the ice-nucleation temperature of rapidly cooled mouse embryos by glycerol and dimethyl sulfoxide. Biophysical journal. 41(1), 1-12.
Ray, B., Souzu, H., Speck, ML (1975) Cryoprotection of Escherichia coli by penetrating and nonpenetrating cryopreservatives. Cryobiology. 12(6), 553.
Renard, P (1991) Cooling and freezing tolerances in embryo of the Pacific oyster, Crassostrea gigas: methanol and sucrose effects. Aquaculture 92(1), 43-57.
Ribes, M., Coma, R., Rossi, S., Micheli, M (2007) Cycle of gonadal development in Eunicella singularis (Cnidaria: Octocorallia): trends in sexual reproduction in gorgonians. Invertebrate Biology. 126(4), 307-317.
Rodríguez-Troncoso, AP., Carpizo-Ituarte, E., Leyte-Morales, GE., Chi-Barragán, G., Tapia-Vázquez, O (2011) Sexual reproduction of three coral species from the Mexican South Pacific. Marine biology. 158(12), 2673-2683.
Ruane, J., Sonnino, A (2006) The role of biotechnology in exploring and protecting agricultural genetic resources. Food & Agriculture Org.
Rudolph, AS., Crowe, JH (1985) Membrane stabilization during freezing: the role of two natural cryoprotectants, trehalose and proline. Cryobiology. 22(4), 367-377.
Santiago-Vázquez, L., Newberger, N., Kerr, R (2007) Cryopreservation of the dinoflagellate symbiont of the octocoral Pseudopterogorgia elisabethae. Marine Biology. 152(3), 549-556.
Santos, RR., Van den Hurk, R., Rodrigues, AP., Costa, SH., Martins, FS., Matos, MH., Celestino, JJ., Figueiredo, JR (2007). Effect of cryopreservation on viability, activation and growth of in situ and isolated ovine early-stage follicles. Animal reproduction science, 99(1), 53-64.
Saragusty, J., Arav, A (2011) Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification. Reproduction. 141(1), 1-19.
Seo, SY., Hwang, SJ., Song, JI (2008) Sexual reproduction of anthoplexaura dimorpha (Gorgonacea: Octocorallia) from Munseom, Jejudo islands, Korea. Animal Cells and Systems, 12(4), 231-240.
Smythe, BM (1967) Sucrose crystal growth. Australian Journal of Chemistry. 21, 1097-1114.
Steponkus, PL (1984) Role of the plasma membrane in freezing injury and cold acclimation. Annual Review of Plant Physiology. 35, 543-584.
Storey, B., Noiles, E., Thompson, K (1998) Comparison of glycerol, other polyols, trehalose, and raffinose to provide a defined cryoprotectant medium for mouse sperm cryopreservation. Cryobiology. 37(1), 46-58.
Strauss, G., Schurtenberger, P., Hauser, H (1986) The interaction of saccharides with lipid bilayer vesicles: Stabilization during freeze-thawing and freeze drying. Biochimica et Biophysica Acta. 858, 169-180.
Suquet, M., Labbé, C., Puyo, S., Mingant, C., Quittet, B., Boulais, M., Haffray, P (2014) Survival, growth and reproduction of cryopreserved larvae from a marine invertebrate, the Pacific oyster (Crassostrea gigas). PloS ONE. 9(4), e93486.
Toner, M., Cravalho, EG., Karel, M (1990) Thermodynamics and kinetics of intracellular ice formation during freezing of biological cells. Journal of Applied Physics. 67(3), 1582-1593.
Tsai, S., Chang, WC., Chavanich, S., Viyakarn, V., Lin, C (2016) Ultrastructural observation of oocytes in six types of stony corals. Tissue and Cell. 48(4), 349-355.
Tsai, S., Chang, WC., Lin, C (2015) Ultrastructural observation of oocytes of six types of stony corals. Tissue and Cell. 48(4), 349-355.
Tsai, S., Chen, J., Spikings, E., Li, J., Lin, C (2014) Degradation of mitochondrial DNA in cryoprotectant-treated hard coral (Echinopora spp.) oocytes. Mitochondrial DNA. Early Online, 1-6.
Tsai, S., Chong, G., Meng, PJ., Lin, C (2017) Sugars as supplemental cryoprotectants for marine organisms. Reviews in Aquaculture. 10.1111/raq.12195.
Tsai, S., Jhuang, Y., Spikings, E., Sung, P.J., Lin, C (2014) Ultrastructural observations of the early and late stages of gorgonian coral (Junceella juncea) oocytes. Tissue and Cell. 46(4), 225-232.
Tsai, S., Kuit, V., Lin, ZG., Lin, C (2014) Application of a functional marker for the effect of cryoprotectant agents on gorgonian coral (Junceella juncea and J. fragilis) sperm sacs. CryoLetters. 35(1), 1-7.
Tsai, S., Lin, C (2009) Effects of cryoprotectant on the embryos of banded coral shrimp (Stenopus hispidus); preliminary studies to establish freezing protocols. CryoLetters. 30(5), 373-381.
Tsai, S., Rawson, DM., Zhang, T (2009) Development of cryopreservation protocols for early stage zebrafish (Danio rerio) ovarian follicles using controlled slow cooling. Theriogenology. 71(8), 1226-1233.
Tsai, S., Spikings, E., Huang, IC., Lin, C (2011) Study on the mitochondrial activity and membrane potential after exposing later stage oocytes of two gorgonian corals (Junceella juncea and Junceella fragilis) to cryoprotectants. CryoLetters. 32(1), 1-12.
Tsai, S., Spikings, E., Kuo, FW., Lin, NC., Lin, C (2010) Use of an adenosine triphosphate assay, and simultaneous staining with fluorescein diacetate and propidium iodide, to evaluate the effects of cryoprotectants on hard coral (Echinopora spp.) oocytes. Theriogenology. 73(5), 605-611.
Tsai, S., Yen, W., Chavanich, S., Viyakarn, V., Lin, C (2015) Development of cryopreservation techniques for gorgonian (Junceella juncea) oocytes through vitrification. PLoS ONE. 10(5), e0123409.
Tsvetkov, T., Naydenova, Z (1987) Activity of ATP synthetase complex after low temperature treatment or freeze-drying of mitochondria isolated from skeletal muscles. Cryobiology. 24(3), 280-284.
Viveiros, A., Maria, AN., Amaral, TB., Orfão, LH., Isau, ZA., Veríssimo‐Silveira, R (2012) Spermatozoon ultrastructure and sperm cryopreservation of the Brazilian dry season spawner fish pirapitinga, Brycon nattereri. Aquaculture Research. 43(4), 546-555.
Wang, HR., Li, XX., Wang, MQ., Clarke, Sm., Gluis, M (2014) The development of oocyte cryopreservation techniques in blue mussels Mytilus galloprovincialis. Fisheries Science 80, 1257-1267.
Wang, WB., Olive, PJW (2000) Effects of cryopreservation on the ultrastructural anatomy of 3‐segment Nereis virens larvae(Polychaeta). Invertebrate Biology. 119(3), 309-318.
Wiggers, HAL (1832) Untersuchungüberdas Mutterkorn, Secale cornutum. Annalen der Pharmacie. 1, 129-182.
Wilkinson, C (2008) Status of coral reefs of the world: 2008. Global Coral Reef Monitoring Network and Reef and Rainforest Research Centre, Townsville, Australia. pp. 296.
Yön, ND., Akbulut, C (2014) Histological Changes in Zebrafish (Danio rerio) Ovaries Following Administration of Bisphenol A. Pakistan Journal of Zoology 46(4).
Yoon, YH., Pope, J., Wolfe, J (1998) The effects of solutes on the freezing properties of and hydration forces in lipid lamellarphases. Biophysical Journal. 74, 1949-1965.
Zelazowska, M (2014) Ultrastructure and histochemistry of the periplasm in oocytes of sturgeons during egg envelope formation. Folia biologica. 62(4), 377-385.
Zhang, GJ., Steponkus, PL (1996) Effect of a glass transformation on the liquidcrystalline to gel phase transition temperature of phospholipids in dehydrated sugar–phospholipid mixtures. Cryobiology. 33, 624-662.
Zhang, K. J., lou, Y. D., zhang, Y. J., Wu, P (1997) studies on cryopreservation of three fishes'embryos. Journal of Fisheries of China. 4, 002.
方永強., 趙維信., 林秋明 (1993) 文昌魚卵母細胞發育成熟激素調控的研究. 中國科學.
王春花., 田永勝 (2009) 牙鮃胚胎冷凍損傷的觀察. 生物學雜誌, 26(6), 5-7.
朱豪磊 (2013) 香港牡蠣(Crassostrea hongkongensis)精子冷凍保存的研究 (Master's thesis, 廣西大學).
李怡青 (2014) 白鞭珊瑚(Junceella fragilis)卵母細胞低溫傷害超顯微結構之研究. 國立東華大學海洋生物科技研究碩士論文.
林政嘉 (2015) 低溫誘發柳珊瑚(Ellisella robusta)卵母細胞型態與超微結構變化之研究.國立東華大學海洋生物科技研究碩士論文.
張永泉., 佳張., 穎佟., 廣香 (2013) 不同發育時期哲羅魚卵黃的超微結構. 動物學雜誌, (2), 249-255.
張威傑 (2013) 六種石珊瑚卵母細胞超微結構解析應用於低溫冷凍保存技術之研究. 國立東華大學海洋生物科技研究碩士論文.
章龍珍., 劉鵬., 莊平 (2008) 超低溫冷凍對西伯利亞鱘 精子形態結構損傷的觀察. 水產學報. 32(4), 558-565.
莊雅婷 (2012) 低溫保存對柳珊瑚(Junceella juncera) 卵母細胞超微結構之影響.國立東華大學海洋生物科技研究碩士論文.
黃意築 (2007) 台灣南部海域紅鞭珊瑚與白鞭珊瑚的生殖與生長變異. 國立屏東科技大學水產養殖系所碩士論文.
趙夢然., 康現江., 穆淑梅., 郭明申., 葛少欽 (2009). 中華鋸齒米蝦卵母細胞卵黃發生的超微結構. 河北大學學報 (自然科學版), 29(3), 318-323.
蔣玉榮 (2016) 皺紋盤鮑 (Haliotis discus) 精子和幼蟲低溫保存的研究 (Master's thesis, 大連海洋大學).
連結至畢業學校之論文網頁點我開啟連結
註: 此連結為研究生畢業學校所提供,不一定有電子全文可供下載,若連結有誤,請點選上方之〝勘誤回報〞功能,我們會盡快修正,謝謝!
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top