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研究生:沈宏璋
研究生(外文):SHEN, HUNG-CHANG
論文名稱:Semaphorin-1a 調控果蠅嗅覺投射神經樹突之分析
論文名稱(外文):Semaphorin-1a Modulates Projection Neuron Dendrites in Drosophila Antennal Lobes
指導教授:游宏祥
指導教授(外文):YU, HUNG-HSIANG
口試委員:簡正鼎林書葦吳嘉霖高智飛
口試委員(外文):CHIEN, CHENG-TINGLIN, SUEWEIWU, CHIA-LINKAO, CHIH-FEI
口試日期:2017-05-19
學位類別:博士
校院名稱:國防醫學院
系所名稱:生命科學研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:英文
論文頁數:130
中文關鍵詞:果蠅嗅小球嗅覺系統投射神經元樹突
外文關鍵詞:drosophilaglomerulusSemaphorin-1aolfactory systemprojection neuronsdendrites
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中文摘要 I
Abstract III
Contents V
List of Figures VIII
List of Tables X
Chapter 1 Introduction 1
1.1 The olfactory system of Drosophila 1
1.2 Development of neuronal connectivity in Drosophila antennal lobes during pupal stages 3
1.3 Molecules regulate development and function of neural circuits 3
Chapter 2 Results 9
2.1 Dendrites of vPNs share different regions in the putative adult AL, then intermingle with adPN and lPN dendrites in the developing AL 9
2.2 DA1 vPN as a model neuron to select molecules that effect dendritic patterns during development stage 10
2.3 Identification of cell surface molecules that participate in DA1 vPN morphogenesis 13
2.4 Loss-of-function of Sema-1a results in mis-targeting of vPN dendrites to the DA3 glomerulus 18
2.5 The adPN, but not lPN, dendrites that project near the DA3 glomerulus also exhibit DA3 dendritic glomerular mis-targeting defects in the Sema-1a loss-of-function mutant 21
2.6 Ectopic expression of Sema-1a in Sema-1a mutant DA3 adPNs causes dendritic mis-projections into the DL3 and DA4l glomeruli 23
2.7 An additional PN dendritic mis-targeting region in the AL is identified in the absence of Sema-1a 25
2.8 Identification of molecules potentially inducing DA3 mis-targeting for VA1d and DC3 adPNs and DA1 lPNs morphogenesis using double knock-down RNAi screen with Sema-1a. 27
2.9 Identification of DA3 mis-targeting in VA1d and DC3 adPNs and DA1 lPNs morphogenesis by knock-down of Sema-1a with mutants. 29
Chapter 3 Discussion 31
Chapter 4 Materials and methods 39
4.1 Fly strains 39
4.2 Fly brain preparation and image processing 39
4.3 Visualization of wild-type, Sema-1a LOF, and Sema-1a rescued PNs. 40
4.4 Quantification of dendritic patterns of DL1 adPNs along the DL-to-VM axis 41
Chapter 5 References 43
Chapter 6 Figures 54
Chapter 7 Tables 94

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