(3.238.186.43) 您好!臺灣時間:2021/02/26 12:42
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:陳又瀅
研究生(外文):CHEN,YOU-YING
論文名稱:光合細菌產類胡蘿蔔素的抗氧化與細胞毒殺之研究
論文名稱(外文):Evaluation for Antioxidative and Cytotoxic Activities of Carotenoids from Photosynthetic Bacteria
指導教授:張瑞璋張瑞璋引用關係
指導教授(外文):CHANG,REY-CHANG
口試委員:林順富吳淑黎呂美津朱惠鈴
口試委員(外文):LIN,SHUEN-FUHWU,SHWU-LILU,MEI-CHINCHU,HEUY-LING
口試日期:2017-07-14
學位類別:碩士
校院名稱:國立高雄海洋科技大學
系所名稱:海洋生物技術研究所
學門:自然科學學門
學類:海洋科學學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:中文
論文頁數:134
中文關鍵詞:光合細菌類胡蘿蔔素抗氧化劑抗酪胺酸酶細胞毒性
外文關鍵詞:photosynthetic bacteriacarotenoidantioxidantantityrosinasecytotoxicity
相關次數:
  • 被引用被引用:1
  • 點閱點閱:128
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
摘要 I
Abstract III
壹、前言 1
貳、文獻回顧 4
2.1 光合細菌 4
2.1.1 光合細菌的分類 5
2.1.2 光合細菌的生理特性 5
2.2 類胡蘿蔔素 5
2.2.1 類胡蘿蔔素的功能 10
2.3 自由基(free radical)與抗氧化 11
2.3.1 自由基(free radical) 11
2.3.2 超氧自由基 11
2.3.3 單重態氧 12
2.3.4 過氧化氫 12
2.3.5 羥基自由基 13
2.3.6 脂質過氧化物 14
2.3.7 過氧亞硝酸自由基 14
2.4 抗氧化劑 16
2.4.1 抗壞血酸 16
2.4.2 生育醇 16
2.4.3 酚類化合物 17
2.4.4 合成酚類抗氧化劑 17
2.4.5 類胡蘿蔔素 18
2.5 抗氧化活性測定 18
2.5.1 DPPH清除自由基能力測定 18
2.5.2 TEAC 總抗氧化能力 19
2.5.3 還原力能力測試 20
2.5.4 亞鐵離子螯合能力測定 21
2.5.5 脂質過氧化抑制能力測定 22
2.5.6 酪胺酸酶抑制活性測定 23
2.6 肝癌 24
2.6.1 肝癌與類胡蘿蔔素 25
2.7 黑色素細胞 25
2.7.1 黑色素細胞與類胡蘿蔔素 27
2.8 發炎反應 28
2.9 細胞凋亡 29
2.9.1 外源性凋亡 30
2.9.2 內源性凋亡 31
2.9.3 細胞活性測定方法 33
2.9.3.1 細胞培養 33
2.9.3.2 細胞冷凍保存 34
2.9.3.3 冷凍細胞活化 34
2.9.3.4 細胞繼代 35
2.9.3.5 細胞存活率分析 35
叁、材料與方法 37
3.1 實驗架構 37
3.2 藥品與材料 38
3.2.1 實驗藥品 38
3.2.2 實驗儀器 39
3.2.3 細胞株 40
3.2.4 培養設備之設計 40
3.3 實驗方法 40
3.3.1 光合細菌之菌種篩選及增殖培養 40
3.3.1.1 樣本採集 40
3.3.1.2 菌種之培養基配方 41
3.3.1.2 菌種篩選與純化分離 42
3.3.2 光合細菌培養實驗 44
3.3.2.1 一般光合細菌的培養 44
3.3.2.2 光合細菌之接菌濃度 44
3.3.3 衡量參數 44
3.3.3.1 光合細菌菌液吸光值之分析 44
3.3.3.2 光合細菌之生長曲線與酸鹼值變化 46
3.3.3.3 光合細菌總類胡蘿蔔素含量之分析 46
3.3.3.4 光合細菌Biomass之分析方法 49
3.3.4 光合細菌產總類胡蘿蔔素之最適培養條件 49
3.3.4.1 明暗週期及通氣厭氧之探討 50
3.3.4.2 最適碳源之探討 51
3.3.4.3 最適氮源之探討 52
3.3.4.4 最適複合式碳氮源 52
3.4 光合細菌總類胡蘿蔔素最適萃取方法 54
3.5 光合細菌之類胡蘿蔔素安定性試驗 55
3.6 抗氧化活性測定 55
3.6.1 DPPH清除自由基能力測定 56
3.6.2 TEAC 總抗氧化能力 56
3.6.3 還原力能力測試 56
3.6.4 亞鐵離子螯合能力測定 57
3.7 酪胺酸酶活性抑制測定 58
3.8 細胞實驗 58
3.8.1 細胞毒殺活性測試 (MTT assay) 58
3.9 光合細菌之類胡蘿蔔素分離純化 59
肆、結果與討論 60
4.1 菌種篩選 60
4.2 菌種鑑定 60
4.3 環境對PSB生長的影響 61
4.4 PSB生長曲線與酸鹼值變化 62
4.5 PSB之類胡蘿蔔素最適萃取方法 62
4.6 PSB產總類胡蘿蔔素最適培養條件探討 63
4.6.1 最適碳源 63
4.6.2 最適氮源 63
4.6.3 最適複合碳氮源 64
4.7 PSB之類胡蘿蔔素萃取率 64
4.8 鹽度對PSB生長的影響 64
4.9 光源種類對PSB生長及類胡蘿蔔素生成的影響 66
4.10 PSB之類胡蘿蔔素安定性試驗 66
4.11 PSB最適培養條件對類胡蘿蔔素的影響 67
4.12 抗氧化活性測定試驗 67
4.12.1 DPPH清除自由基能力測定 67
4.12.2 TEAC 總抗氧化能力測定 68
4.12.3 還原力能力測試 69
4.12.4 亞鐵離子螯合能力測定 69
4.13 酪胺酸酶活性抑制測定 69
4.14 細胞實驗 70
4.14.1 細胞毒殺活性試驗 70
4.15 TC-S2 分離純化 71
五、結論 72
參考文獻 75


吳育甄、林峰右、黃致中、葉信利,光合菌Rhodovulum sulfidophilum固氮基因之選殖。水試專訊,2014,46,7-9
黃世鈴、楊豐隆、黃麗玲,在水產養殖場使用光合菌的益處。水試專訊,2013,44,48-50
黃貴閩,莢膜紅假單胞菌類胡蘿蔔素的穩定性能初探。福州大學學報, 1000- 2243 (2006) 04- 0616- 04。
劉如林,刁虎欣,梁鳳來,等. 光合細菌及其應用[M]. 北京: 中國農業科技出版社,1991: 95.
鄭榮梁、魏耀揮、趙崇義、黃中洋、陳建春,自由基生物醫學,藝軒圖書出版社 (2013) 。
簡道南,會進行光合作用的神奇細菌--光合細菌(Photosynthetic Bacteria 簡稱PSB)。臺肥季刊,2013,39-44



Arathi, B.P., Sowmya, P.R.-R., Vijay, K., Baskaran, V., and Lakshminarayana, R. (2015). Metabolomics of carotenoids: The challenges and prospects – A review. Trends in Food Science & Technology 45, 105–117.
Asgari, M.M., Brasky, T.M., and White, E. (2012). Association of Vitamin A and Carotenoid Intake with Melanoma Risk in a Large Prospective Cohort. Journal of Investigative Dermatology 132, 1573–1582.
Baron, M., Davies, S., Alexander, L., Snellgrove, D., and Sloman, K.A. (2008). The effect of dietary pigments on the coloration and behaviour of flame-red dwarf gourami, Colisa lalia. Animal Behaviour 75, 1041–1051.
Bauernfeind, J. Christopher, ed. Carotenoids as Colorants and Vitamin A Precursors: Technological and Nutritional Applications. Food Science and Technology. New York, N.Y: Academic Press, 1981.
Bergey, D. H, and John G Holt. Bergey’s Manual of Determinative Bacteriology. Baltimore: Williams & Wilkins, 1993.
Blair, I.A. Lipid hydroperoxide-mediated DNA damage. Experimental Gerontology , 2001, 36, 1473–1481.
Blokhina, O., Virolainen, E., and Fagerstedt, K.V. (2003). Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann. Bot. 91 Spec No, 179–194.
Bone, R.A., and Landrum, J.T. (1992). [33] Distribution of macular pigment components, zeaxanthin and lutein, in human retina. Methods in Enzymology 213, 360–366.
Canabady-Rochelle, L.L.S., Harscoat-Schiavo, C., Kessler, V., Aymes, A., Fournier, F., and Girardet, J.-M. (2015). Determination of reducing power and metal chelating ability of antioxidant peptides: Revisited methods. Food Chemistry 183, 129–135.
Cantrell, A., McGarvey, D.J., George Truscott, T., Rancan, F., and Böhm, F. (2003). Singlet oxygen quenching by dietary carotenoids in a model membrane environment. Archives of Biochemistry and Biophysics 412, 47–54.
Chang, T.-S. (2009). An updated review of tyrosinase inhibitors. Int J Mol Sci 10, 2440–2475.
Chen, D., Han, Y., and Gu, Z. (2006). Application of statistical methodology to the optimization of fermentative medium for carotenoids production by Rhodobacteraceae sphaeroides. Process Biochemistry 41, 1773–1778.
Cooper, D.A. (2004). Carotenoids in Health and Disease: Recent Scientific Evaluations, Research Recommendations and the Consumer. J. Nutr. 134, 221S–224S.
Dreher, D., and Junod, A.F. (1996). Role of oxygen free radicals in cancer development. Eur. J. Cancer 32A, 30–38.
Fenton, H.J.H. (1894). LXXIII.—Oxidation of tartaric acid in presence of iron. J. Chem. Soc., Trans. 65, 899–910.
Griendling, K.K., and FitzGerald, G.A. (2003). Oxidative Stress and Cardiovascular Injury. Circulation 108, 1912–1916.
Huang, C.-S., Liao, J.-W., and Hu, M.-L. (2008). Lycopene Inhibits Experimental Metastasis of Human Hepatoma SK-Hep-1 Cells in Athymic Nude Mice. J. Nutr. 138, 538–543.
Huang, C.-S., Shih, M.-K., Chuang, C.-H., and Hu, M.-L. (2005). Lycopene inhibits cell migration and invasion and upregulates Nm23-H1 in a highly invasive hepatocarcinoma, SK-Hep-1 cells. J. Nutr. 135, 2119–2123.
Kim, J.K. Lee, B.K. (2000). Mass production of Rhodopseudomonas palustris as diet for aquaculture Aquacultural Engineering 23 281–293.
Koller, M., Muhr, A., and Braunegg, G. (2014). Microalgae as versatile cellular factories for valued products. Algal Research 6, 52–63.
Koppenol, W.H. (2001). The Haber-Weiss cycle--70 years later. Redox Rep. 6, 229–234.
Kotake-Nara, E., Kushiro, M., Zhang, H., Sugawara, T., Miyashita, K., Nagao, A. (2001). Carotenoids affect proliferation of human prostate cancer cells. J Nutr 131:3303-3306.
Krinsky, N.I., and Johnson, E.J. (2005). Carotenoid actions and their relation to health and disease. Molecular Aspects of Medicine 26, 459–516.
Kuo, F.-S., Chien, Y.-H., and Chen, C.-J. (2012). Effects of light sources on growth and carotenoid content of photosynthetic bacteria Rhodopseudomonas palustris. Bioresour. Technol. 113, 315–318.
LaClair, D. D. (2006). Optimization and Characterization of the Growth of the Photosynthetic Bacterium Blastochloris Viridis and a Brief Survey of Its Potential as a Remediative Tool. ProQuest.
Larimer, F.W., Chain, P., Hauser, L., Lamerdin, J., Malfatti, S., Do, L., Land, M.L., Pelletier, D.A., Beatty, J.T., Lang, A.S., et al. (2004). Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris. Nat Biotech 22, 55–61.
Lin, C.L ., Kao, J.H., (2008). Epidemiology of Hepatocellular Carcinoma. J. Chinese Oncol. 24(5), 277-281 .
Liu, W.-S., Kuan, Y.-D., Chiu, K.-H., Wang, W.-K., Chang, F.-H., Liu, C.-H., and Lee, C.-H. (2013). The Extract of Rhodobacter sphaeroides Inhibits Melanogenesis through the MEK/ERK Signaling Pathway. Marine Drugs 11, 1899–1908.
Llorente, B., Martinez-Garcia, J.F., Stange, C., and Rodriguez-Concepcion, M. (2017). Illuminating colors: regulation of carotenoid biosynthesis and accumulation by light. Current Opinion in Plant Biology 37, 49–55.
Lobo, V., Patil, A., Phatak, A., and Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 4, 118–126.
McCord, J.M. (2000). The evolution of free radicals and oxidative stress. Am. J. Med. 108, 652–659.
Miki, W. (1991). Biological functions and activities of animal carotenoids. Pure Appl. Chem., PAC 63, 141–146.
Miller, S.B. (2006). Prostaglandins in Health and Disease: An Overview. Seminars in Arthritis and Rheumatism 36, 37–49.
Miyamoto, S., Martinez, G.R., Medeiros, M.H.G., and Di Mascio, P. (2014). Singlet molecular oxygen generated by biological hydroperoxides. J. Photochem. Photobiol. B, Biol. 139, 24–33.
Niranjana, R., Gayathri, R., Nimish Mol, S., Sugawara, T., Hirata, T., Miyashita, K., and Ganesan, P. (2015). Carotenoids modulate the hallmarks of cancer cells. Journal of Functional Foods 18, Part B, 968–985.
Nisar, N., Li, L., Lu, S., Khin, N.C., and Pogson, B.J. (2015). Carotenoid Metabolism in Plants. Molecular Plant 8, 68–82.
Nishino, H. (1997). Cancer prevention by natural carotenoids. J. Cell. Biochem. Suppl. 27, 86–91.
Oikawa, S., Nishino, K., Oikawa, S., Inoue, S., Mizutani, T., and Kawanishi, S. (1998). Oxidative DNA damage and apoptosis induced by metabolites of butylated hydroxytoluene. Biochem. Pharmacol. 56, 361–370.
Oliveira, S., Coelho, P., Prudêncio, C., Vieira, M., Soares, R., Guerreiro, S.G., and Fernandes, R. (2016). Melanoma and obesity: Should antioxidant vitamins be addressed? Life Sciences 165, 83–90.
Qi, Z., Zhang, X.-H., Boon, N., and Bossier, P. (2009). Probiotics in aquaculture of China — Current state, problems and prospect. Aquaculture 290, 15–21.
Robak, J., and Gryglewski, R.J. (1988). Flavonoids are scavengers of superoxide anions. Biochem. Pharmacol. 37, 837–841.
Roy, S., Llewellyn, C.A., Egeland, E.S., and Johnsen, G. (2011). Phytoplankton Pigments: Characterization, Chemotaxonomy and Applications in Oceanography (Cambridge University Press).
Scheibmeir, H.D., Christensen, K., Whitaker, S.H., Jegaethesan, J., Clancy, R., and Pierce, J.D. (2005). A review of free radicals and antioxidants for critical care nurses. Intensive Crit Care Nurs 21, 24–28.
Sheikhpour, R. (2013). Diabetes and Oxidative Stress: The Mechanism and Action. Iranian Journal of Diabetes and Obesity 5, 40–45.
Shimada, K. (Yamaguchi W.U., Fujikawa, K., Yahara, K., and Nakamura, T. (1992). Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of Agricultural and Food Chemistry (USA).
Singh, M., Kapoor, A., and Bhatnagar, A. (2015). Oxidative and reductive metabolism of lipid-peroxidation derived carbonyls. Chem Biol Interact 234, 261–273.
Slaga, T.J. (1995). Inhibition of skin tumor initiation, promotion, and progression by antioxidants and related compounds. Crit Rev Food Sci Nutr 35, 51–57.
Snodderly, D.M. (1995). Evidence for protection against age-related macular degeneration by carotenoids and antioxidant vitamins. Am. J. Clin. Nutr. 62, 1448S–1461S.
Stajčić, S., Ćetković, G., Čanadanović-Brunet, J., Djilas, S., Mandić, A., and Četojević-Simin, D. (2015). Tomato waste: Carotenoids content, antioxidant and cell growth activities. Food Chemistry 172, 225–232.
Sugawara, T., Ganesan, P., Li, Z., Manabe, Y., and Hirata, T. (2014). Siphonaxanthin, a Green Algal Carotenoid, as a Novel Functional Compound. Mar Drugs 12, 3660–3668.
Szabó, C., Ischiropoulos, H., and Radi, R. (2007). Peroxynitrite: biochemistry, pathophysiology and development of therapeutics. Nat Rev Drug Discov 6, 662–680.
Torres-Fuentes, C., Alaiz, M., and Vioque, J. (2012). Iron-chelating activity of chickpea protein hydrolysate peptides. Food Chemistry 134, 1585–1588.
Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T.D., Mazur, M., and Telser, J. (2007). Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 39, 44–84.
Van den Berg, R., Haenen, G.R.M.M., van den Berg, H., and Bast, A. (1999). Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures. Food Chemistry 66, 511–517.
Wang, H., Yang, A., Zhang, G., Ma, B., Meng, F., Peng, M., and Wang, H. (2017). Enhancement of carotenoid and bacteriochlorophyll by high salinity stress in photosynthetic bacteria. International Biodeterioration & Biodegradation 121, 91–96.
Wang, W., Asimakopoulos, A.G., Abualnaja, K.O., Covaci, A., Gevao, B., Johnson-Restrepo, B., Kumosani, T.A., Malarvannan, G., Minh, T.B., Moon, H.-B., et al. (2016). Synthetic Phenolic Antioxidants and Their Metabolites in Indoor Dust from Homes and Microenvironments. Environ. Sci. Technol. 50, 428–434.
Wu, P., Zhang, G., Li, J., Lu, H., and Zhao, W. (2012). Effects of Fe2+ concentration on biomass accumulation and energy metabolism in photosynthetic bacteria wastewater treatment. Bioresource Technology 119, 55–59.
Yang, A., Zhang, G., Yang, G., Wang, H., Meng, F., Wang, H., and Peng, M. (2017). Denitrification of aging biogas slurry from livestock farm by photosynthetic bacteria. Bioresource Technology 232, 408–411.
Yen, G.-C., Duh, P.-D., and Tsai, H.-L. (2002). Antioxidant and pro-oxidant properties of ascorbic acid and gallic acid. Food Chemistry 79, 307–313.
Zhou, Q., Zhang, P., and Zhang, G. (2014). Enhancement of cell production in photosynthetic bacteria wastewater treatment by low-strength ultrasound. Bioresource Technology 161, 451–454.
Zhou, Q., Zhang, P., Zhang, G., and Peng, M. (2015). Biomass and pigments production in photosynthetic bacteria wastewater treatment: Effects of photoperiod. Bioresource Technology 190, 196–200.
Venkata Mohan, S., and Devi, M.P. (2014). Salinity stress induced lipid synthesis to harness biodiesel during dual mode cultivation of mixotrophic microalgae. Bioresource Technology 165, 288–294.
Deepti D. Dhere, S. M. Dharmadhikari. (2015). Optimization of Carotenoid Production by Paracoccus beibuensis Isolated from Lonar Crater. International Journal of Science and Research, 2319-7064.
Chan, Y.Y., Kim, K.H., and Cheah, S.H. (2011). Inhibitory effects of Sargassum polycystum on tyrosinase activity and melanin formation in B16F10 murine melanoma cells. Journal of Ethnopharmacology 137, 1183–1188.

電子全文 電子全文(網際網路公開日期:20220701)
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關點閱論文
 
系統版面圖檔 系統版面圖檔