近年來由於全球溫室效應影響，登革熱為目前世界上廣為蔓延之疾病之一，發病率在全球大幅上升，而目前在預防及治療登革熱策略上，極少有疫苗或藥劑，因此開發治療抗登革熱藥物為刻不容緩之事。
印度塔樹 (Polyalthia longifolia) 為番荔枝科 (Annonaceae) 植物，是一種多年生常綠喬木，廣泛分布於熱帶及亞熱帶地區，於傳統上用來治療糖尿病、高血壓、皮膚方面等疾病，並具有抗發炎、抗癌之活性。
過去本植物曾發現內含雙萜類與生物鹼類之化合物，本研究首次發現四個新化合物為雙萜類與生物鹼類化合物相互鏈結之新化合物。目前共鑑定出三個新clerodane 雙萜類與aporphine 生物鹼鏈結化合物，Polyalongirin A-C (1-3) 與一個新clerodane 雙萜類與proaporphine 生物鹼鏈結化合物Polyalongirin D (4)。上述所有化合物皆利用管柱色層分析、高效能液相層析儀與再結晶法等進行分離純化，其結構透過各種物理數據 (紅外線光譜、質譜、紫外線光譜、旋光值)，配合核磁共振圖譜，解析其化學結構。再利用X-ray與圓二色光譜確定其絕對立體結構。
在活性試驗中，對登革熱病毒進行初步測試，針對人類肝癌Huh-7
細胞株，以登革熱第二型 (strain 16681) 病毒感染後，分別以Polyalongirin A-C (1-3) 進行登革病毒細胞感染活性試驗，實驗結果發現Polyalongirin B (2) 對NS2B蛋白之表現量具有明顯抑制效果，未來將進一步探討其作用機轉及動物試驗。

In the recent years, due to the greenhouse effect, dengue fever is the most widely spread diseases and its morbidity increased significantly in the world. To date, there are less vaccine or pharmacy to prevent and treat dengue fever, so the development of anti-dengue fever drugs was urgent.
Polyalthia longifolia, belonging to the Annonaceae family, is an everygreen tree that widely distributed in tropical and subtropics region. The species is traditionally used for the treatment of diabetes, hypertension and skin disease and exhibits anti-inflammatory and anticancer activities.
In the past, it had been found diterpenes and alkaloids compounds in this plant. In this study, we found four compounds with diterpene and alkaloid linkage for the first time, containing diterpene and alkaloid compounds linked to each other. Now, we have identified three novel clerdone diterpenes and aporphine alkaloids linked compounds, Polyalongirin A-C (1-3) with a novel clerdone diterpenes and proaporphine alkaloids linked compound, Polyalongirin D (4). Further purification was carried out by column chromatography, high performance liquid chromatograph and recrystallization.
All structures of the compounds were elucidated by NMR spectra and physical data ( IR, mass spectrometry, ultraviolet, optical rotation ). Their absolute structure were determined by X-ray and CD spectrum.
In the activity test, preliminary testing of dengue virus, against the human hepatocyte cell line Huh-7 with dengue fever type 2 (strain 16681) virus infection. Selected compounds were then investigated for their ability to inhibit dengue NS2B protein. Among these compounds, Polyalongirin B (2) exhibited significant inhibitory effects against NS2B protein expression in the infected Huh-7 cells replication. We will further explore the mechanism of action and animal experiments in the future.

摘要....................................................I
Abstract...............................................II
誌謝....................................................Ⅲ
目錄....................................................Ⅳ
圖表目錄.................................................Ⅴ
壹、前言................................................1
一、緒言................................................1
二、植物簡介............................................8
三、研究動機...........................................13
貳、文獻回顧...........................................14
一、番荔枝科暗羅屬.....................................14
二、抗登革熱病毒.......................................30
參、材料與方法.........................................32
一、化合物分離與純化....................................32
(一) 實驗藥品與儀器..................................32
(二) 實驗方法.......................................34
(三) 結構之鑑定.....................................38

二、登革熱病毒之NS2B蛋白表現試驗.........................40
肆、結果 ...........................................41
一、Polyalongirin A (1) 之結構解析......................42
二、Polyalongirin B (2) 之結構解析......................56
三、Polyalongirin C (3) 之結構解析......................69
四、Polyalongirin D (4) 之結構解析......................82
五、Circular Dichroism Spectrum結果....................95
六、登革病毒初步活性試驗結果.............................96
伍、結論................................................97
參考文獻................................................99
作者簡介...............................................106
附錄...................................................107

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