本研究主要利用簡單、快速之方法來製作紙基流體檢測試片。在傳統的比色法中，反應試紙可以直接拿來與色階表作比對，因為樣本濃度的不同使得檢測試片在經過一連串的化學反應下，呈現的顏色強度會有所不同；然而檢測片上產生的顏色變化會隨著時間而衰變，因而須在特定的某段時間內，將檢測片與色階表作比對而得知樣本濃度值。對羥苯甲酸甲酯(Methyl p-hydroxybenzoate, Methyl Paraben)廣泛地被運用來當作食品、化妝品及藥品中的防腐劑，且對黴菌、酵母菌及細菌具有良好的抑菌效果。但此化合物恐引發接觸性皮膚炎和藥物過敏，也有報告指出其會促進乳癌的發生。為了更有效率的檢測對羥苯甲酸甲酯，而開發紙基檢測試片。實驗方法主要利用Fenton反應產生的氫氧自由基來氧化對羥苯甲酸甲酯成為苯酚的型態，接著與Fe2+進行錯合，而產生黃綠色之錯化物。分光光度法中反應五至十分鐘後，可達到最高的吸光值，對羥苯甲酸甲酯標準品在200-1000 ppm的濃度下得廻歸方程式為y = 0.0007x – 0.1429，R2=0.9918，偵測極限可達200 ppm。而在紙基分析法中使用WhatmanTM濾紙作為實驗工具，反應四至五分鐘後具有最深之顏色，並以拍攝照片的方式記錄色階，再利用Vision Assistant 軟體讀取紅色(R)、綠色(G)、藍色(B)值，因反應後產物為黃綠色，依據光學原理選用R+G之數值進行分析。對羥苯甲酸甲酯標準品在1000-5000 ppm的濃度下得到廻歸方程式為y (R+G) = -0.031 x + 451.3，R2=0.996，紙基檢測片偵測極限約為1000 ppm。將開發之紙基檢測片應用於真實化妝品中，並與認證實驗室依衛福部公告之方法所得相比較，約可達85 %相符合度，因此此新方法具有潛力以應用於化妝品中防腐劑之檢測。由於在化妝品的限量規範中，最多可添加量為1％(10000 ppm)，且市售化妝品中含量約為1000~5000 ppm，因此利用此簡易檢測裝置可快速得知樣本中對羥苯甲酸甲酯的濃度，且不需花費高成本及使用昂貴的儀器，即可達到半定量的效果。

This thesis aims to fabricate a paper-based microfludic device with simplicity and fast speed. In conventional colormetric method, test strip would be compared with laves table after sequential color developments through chemical reactions. Color on test strip would change over time, which necessitate the comparison with laves table within predefined time frame, in order to quantify the contents by color development. Methyl p-hydroxybenzoate, commonly known as Methyl Paraben, has been widely used in foods, cosmetics, and pharmaceuticals, because its fine inhibitory effect on molds, yeasts, and bacterial. However, this compound could cause contact dermatitis and drug allergy, also reported as associated with breast cancer.
In order to detect methyl paraben contents, the principles of Fenton reaction were applied. Hydroxyl radical via Fenton mechanism would oxidize methyl paraben into phenol configuration, further complexed with Fe2+ to yield yellow-green coordination compound. Maximum absorbance was obtained after reaction of 5-10 minute in spectrophometric method, regression equation as y = 0.0007x – 0.1429, R2=0.9918, was acquired with standard solutions in 200-1000 ppm of methyl paraben. The limit of detection (LOD) by spectrophometric method was determined as 200 ppm. Paper-based device was designed utilizing WhatmanTM filter paper, and color developed via 4-5 minute of reaction on paper. Color laves were photographed and analyzed with Vision Assistant software. R (red), G (green), and B (blue) values were recorded, and R+G value was chosen to represent the color on paper-based device in accordance with principles of optics. Regression equation as y (R+G) = -0.031 x + 451.3, R2=0.996, was acquired with standard solutions in 1000-5000 ppm of methyl paraben. The limit of detection (LOD) by paper-based device was determined as 1000 ppm.
The device was applied to commercial cosmetics with approximately 85% of pertinence, in comparison with the results carried out by accredited laboratory via standard method (by TFDA), which indicates the application potential of this paper-based device for cosmetics. The maximum allowable addition of methyl paraben in cosmetics has been set at 1% (10000 ppm), and was commonly found between 1000 to 5000 ppm, which highlights the applicability of this simple detection device. Semi-quantitative results could be drawn without high cost nor sophisticated instruments.

目錄
中文摘要 I
Abstract III
誌謝 V
目錄 VI
圖目錄 X
表目錄 XII
第1章 前言 1
第2章 文獻回顧 2
2.1微流體設備 2
2.1.1紙基分析設備 2
2.1.2微流體紙基分析設備製造技術 3
2.1.3紙基設備的應用 6
2.2對羥苯甲酸酯類 7
2.2.1對羥基苯甲酸甲酯之應用 7
2.2.2對羥苯甲酸酯之抑菌能力 11
2.2.3國內外化妝品之對羥苯甲酸酯限量標準 12
2.2.4衛生福利部公告對羥苯甲酸甲酯之檢測方法 12
2.3 Fenton反應 17
2.4 Fenton反應之影響因子 21
2.4.1 pH值 21
2.4.2 H2O2添加量 21
2.4.3鐵離子濃度影響 21
2.5 Fenton反應之應用 22
第3章 材料與方法 23
3.1研究目的 23
3.2實驗材料 23
3.2.1 藥品 23
3.2.2 材料與儀器 25
3.2.3檢液及反應試劑製備 25
3.3分光光度法 26
3.3.1實驗原理 26
3.3.2檢測實驗步驟 26
3.3.3全波長掃描 26
3.3.4時間穩定性試驗 26
3.3.5對羥苯甲酸甲酯之檢量線(分光光度法) 27
3.3.6干擾性試驗 27
3.4紙基測定法 27
3.4.1紙基設計 27
3.4.2實驗步驟 27
3.4.3最佳反應時間 27
3.4.4氯化鈉最佳塗覆濃度 28
3.4.5對羥苯甲酸甲酯之檢量線(紙基測定法) 28
3.5真實樣品之檢測 28
3.5.1高效能液相層析分析法(衛福部公告字號1021950692) 28
3.5.2分光光度法及紙基測定法 29
3.5.3真實樣品回添試驗 30
第4章 結果與討論 33
4.1分光光度法 33
4.1.1全波長掃描 33
4.1.2 對羥苯甲酸甲酯之檢量線(分光光度法) 33
4.1.3時間穩定性 33
4.1.4干擾性試驗 34
4.2紙基測定法 40
4.2.1對羥苯甲酸甲酯之檢量線(紙基測定法) 40
4.2.2最佳反應時間 40
4.2.3氯化鈉最佳塗覆濃度 40
4.3實際樣品檢測結果 47
4.3.1分光光度法與HPLC之結果比較 47
4.3.2紙基分析法與HPLC之結果比較 47
4.3.3對羥苯甲酸甲酯與各種分析方法之比較 47
第5章 結論 52
第6章 參考文獻 53
作者簡介 61

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