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研究生:陳詣蓁
研究生(外文):Chen, Yi-Zhen
論文名稱:台灣藜殼調節倉鼠血脂功效之探討
論文名稱(外文):Regulation effect of djulis hull on blood lipids in hyperlipidemic hamsters
指導教授:陳與國
指導教授(外文):Chen, Yu-Kuo
口試委員:林季千陳樺翰羅至佑
口試日期:2017-06-26
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:食品科學系所
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:中文
論文頁數:71
中文關鍵詞:台灣藜殼高血脂症三酸甘油酯總膽固醇
外文關鍵詞:Djulis hullhyperlipidemictriglyceridetotal cholesterol
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根據行政院衛福部最新統計資料顯示,2015年台灣十大死因中,與血脂異常相關的心臟疾病與腦血管疾病分別位居第二、三位,而根據文獻指出,導致此類心血管相關疾病之最主要因素為動脈粥狀硬化 (Atherosclerosis),而造成動脈粥狀硬化的主因是血液中膽固醇濃度過高。歷年來罹患心血管相關疾病的人口持續增加,因此健康養生的飲食與適當的運動,已成為國人必須重視的課題之一。台灣藜 (Chenopodium formosanum),俗稱紅藜,為藜麥屬,是台灣特有原生種植物,而台灣藜最主要的抗氧化成分-台灣藜殼 (DH),含有許多機能性成分,根據文獻指出,其成分中的水溶性纖維、多酚化合物,皆具有降血脂的功效。因此本實驗目的在探討DH調節血脂濃度來達到預防動脈粥狀硬化的功效。實驗分為五組:控制組 (C)、高膽固醇組 (高油脂飼料含0.2%膽固醇;HFC)、低劑量樣品組 (HFC飼料並管餵50 mg/kg/day DH;LDH)、中劑量樣品組(HFC飼料並管餵100 mg/kg/day DH;MDH)、高劑量樣品組 (HFC飼料並管餵250 mg/kg/day DH;HDH)。實驗進行六週,結果顯示HFC組血清中三酸甘油酯 (TG:356±16 mg/dl)、總膽固醇 (TC:463±88 mg/dl) 及低密度脂蛋白膽固醇 (LDL-C:153±15 mg/dl) 含量顯著高於C組 (TG:128±15 mg/dl;TC:130±10 mg/dl;LDL-C:21.3±5 mg/dl)。經過DH處理後,血清中TG (LDH:260±65 mg/dl;MDH:226±70 mg/dl;HDH:201±29 mg/dl)、TC (LDH:280±49 mg/dl;MDH:277±37 mg/dl;HDH:251±39 mg/dl) 及LDL-C (LDH:133±55 mg/dl;MDH:133±1 mg/dl;HDH:79±33 mg/dl) 顯著降低HFC血清中TG、TC及LDL-C含量。此外,DH處理組肝臟及糞便中TG與TC含量皆低於HFC組。上述結果推論,於HFC餵飼倉鼠模式中,給予DH具降血脂之潛力,對於預防高血脂症是一種有益的功能性食品。
Heart disease and cerebrovascular disease are the second and third leading cause of death in Taiwan in 2015. Many studies showed that the most important factor leading to cardiovascular-related diseases is atherosclerosis, and the main caused by atherosclerosis is high blood cholesterol levels. Over the years, the risk of cardiovascular disease associated with population continues to increase, so the healthy diet and exercise have become one of the important issues. Djulis (Chenopodium formosanum) is a native cereal plant in Taiwan, and the djulis hull (DH) was found to be its major source of antioxidant compound. Studies showed that it possesses several active components with hypolipidemic activities, such as dietary fiber and polyphenol compounds. The objective of this investigation was to evaluate the regulation effects of DH in blood lipid levels to achieve the prevention of atherosclerosis effect. Five weeks old hamsters were randomly divided into five groups: (1) control group (C), (2) hyperlipidemic group (high fat diet containing 0.2% cholesterol; HFC), (3) low-dose DH group (HFC diet and treated with 50 mg/kg/day DH; LDH), medium-dose DH group (HFC diet and treated with 100 mg/kg/day DH; MDH), high-dose DH group (HFC diet and treated with 250 mg/kg/day DH; HDH). After 6 weeks of feeding study, the results showed that levels of triglyceride (TG: 356±16 mg/dl), total cholesterol (TC: 463±88 mg/dl) and low-density lipoprotein cholesterol (LDL-C: 153±15 mg/dl) in serum were significantly increased in HFC group compared with C group (TG: 128±15 mg/dl; TC: 130±10 mg/dl; LDL-C: 21.3±5 mg/dl). After DH administration, the results showed that levels of TG (LDH: 260±65 mg/dl; MDH: 226±70 mg/dl; HDH: 201±29 mg/dl), TC (LDH: 280±49 mg/dl; MDH: 277±37 mg/dl; HDH: 251±39 mg/dl) and LDL-C (LDH: 133±55 mg/dl; MDH: 133±1 mg/dl; HDH: 79±33 mg/dl) in serum were significantly reduced by DH administration compared with those of HFC group. Moreover, levels of TG and TC in liver and feces were lower than HFC group. These results indicate that DH possesses hypolipidemic potential to be a benefit functional food to prevent high fat/high cholesterol diet-induced hyperlipidemia.
摘 要 I
Abstract III
謝 誌 V
目 錄 VII
圖目錄 IX
表目錄 XI
壹、前言 1
貳、文獻回顧 2
一、血管異常與動脈粥狀硬化之形成 2
二、脂質代謝 3
(一) 三酸甘油酯 (Triglyceride) 3
(二) 膽固醇 (Cholesterol) 5
(三) 脂蛋白 (Lipoprotein) 6
三、低密度脂蛋白與氧化修飾型低密度脂蛋白 (OxLDL) 8
四、高血脂症 9
(一) 簡介 9
(二) 危險因子 10
五、高血脂倉鼠動物模式 11
六、台灣藜殼 11
(一) 藜麥簡介 11
(二) 台灣藜殼成分與功效 12
參、材料與方法 25
一、實驗架構 25
二、實驗倉鼠飼養進程表 26
三、實驗材料與儀器設備 27
(一) 實驗樣品 27
(二) 化學藥品、溶劑 27
(三) 生化分析試紙 27
(四) 酵素套組 28
(五) 動物飼料用材料 28
(六) 儀器設備 28
四、實驗方法 30
(一) 動物飼養與分組 30
(二) 禁食狀態血液之採集與處理 31
(三) 動物犧牲與檢體收集之分析 32
肆、結果與討論 37
一、實驗期間倉鼠之生長情形及攝食量變化 37
二、血清中三酸甘油酯 (TG) 濃度分析 38
三、血清中總膽固醇 (TC) 濃度分析 39
四、血清中丙氨酸轉氨酶 (ALT) 濃度分析 39
五、血清中葡萄糖 (GLU) 濃度分析 40
六、血清中各種脂蛋白膽固醇含量分析 40
七、血清中低密度脂蛋白氧化遲滯期之分析 41
八、臟器組織重量 42
九、肝臟外觀及病理組織分析 43
十、肝臟中三酸甘油酯及總膽固醇濃度 44
十一、糞便中三酸甘油酯及總膽固醇含量 44
伍、結論 45
陸、實驗圖表 46
柒、參考文獻 64
行政院衛生福利部。2003。高血脂防治手冊。
行政院衛生福利部。2016。中華民國104年國人主要死因統計資料。
吳佩樺。2008。不同品種及生長季節之紅藜抗氧化活性的探討。國立屏東科技大學食品科學系碩士論文。
吳亮宜、簡永亮、蘇正元、何承璋、盧崇如、孫璐西。2003。市售綠茶飲品對倉鼠血脂及低密度脂蛋白氧化之影響。臺灣營養學會雜誌。28卷。158-167。
林俞芳。2014。台灣藜與白藜種子皂素成分與抑菌效能之比較。國立屏東科技大學食品科學研究所。碩士論文。
徐松蘭。2004。台灣五葉松對倉鼠降血脂作用的影響。國立屏東科技大學食品科學研究所。碩士論文。
徐振訓。2007。以高血脂動物模式評估紫蘇油與納豆激酶對調節血脂功能之影響。亞洲大學生物科技與生物資訊學系。碩士論文。
郭耀綸、楊遠波、蔡碧仁、葛孟杰。2008。紅藜推廣手冊。國立屏東科技大學。屏東。
劉新裕、林俊義、張成國。2002。藥用植物專輯。藥用植物對人體健康之維護與貢獻。行政院農業委員會農業試驗所編印,36頁。
蔡蕙芸。2007。龍眼花水翠物對高果糖誘發代謝症候群大鼠之影響。國立台灣大學生物資源暨農學院食品科技研究所。碩士論文。
蕭明熙、黃渝棋、張寶文、高寰宇、林怡伶。2001。從調節血脂到抗氧化與抗動脈粥狀硬化。榮民總醫院教學研究部。台北。
簡永亮。2010。「靈芝-豆科」發酵產物之抗氧化活性及其對倉鼠脂質之影響。國立台灣大學食品科技研究所。博士論文。
Arbeeny CM, Meyers DS, Bergquist KE, Gregg RE. 1992. Inhibition of fatty acid synthesis decreases very low density lipoprotein secretion in the hamster. J Lipid Res. 6: 843-851.
Bell RM, Coleman RA. 1980. Enzymes of glycerolipid synthesis in eukaryotes. Annu Rev Biochem. 49: 459-487.
Bezard J, Bugaut M. 1986. In: Fat absorption. Vol. 1. Boca Ration, pp 119-158.
Brindley DN. 1991. Metabolism of triacylglycerols. In: Biochemistry of lipids, lipoproteins and membrances. Amsterdam: Elsevier, pp 171-203.
Chen WJ, Anderson JW, Jennings D. 1984. Propionate may mediate the hypochlesterolemic effects of certain soluble plant fibers in cholesterol-fed rats. Proc. Soc. Exp. Biol. Med. 175(2): 215-218.
Coleman RA, Haynes EB. 1984. Hepatic monoacylglycerol acyltransferase. Characterization of an activity associated with the suckling period in rats. J Biol Chem. 259(14): 8934-8938.
Dahlen G, Guyton JR, Attar M, Farmer JA, Kautz JA, Gotto AJ. 1986. Association of levels of lipoprotein Lp (a), plasma lipids, and other lipoproteins with coronary artery disease documented by angiography. Circulation. 74(4): 758-765.
Diaz MN, Frei B, Vita JA, Keaney JF Jr. 1997. Antioxidants and atherosclerotic heart diease. N Engl J Med. 337(6): 408-416.
Dichtl W, Nilsson L, Goncalves I. 1999. Very low-density lipoprotein activates nuclear factor-kB in endothelial cells. Circ Res. 84(9): 1085-1094.
Ding YA, Shen MMS, Shieh SM, Li YB. 1984. High density lipoproteins (HDL) and low density lipoproteins (LDL) subfractionations on ischemic cerebral vascular disease in Chinese. J Formosa Med Assoc. 83: 1076-1086.
Esterbauer H, Gebicki J, Puhl H, Jürgens G. 1992. The role of lipid peroxidation and antioxidants in oxidative modification of LDL. Free Radic Biol Med. 13(4): 341-349.
Fielding CJ, Fielding PE. 1995. Molecular physiology of reverse cholesterol transport. J Lipid Res. 36(2): 211-328.
Frankel EN, Bosanek CA, Meyer AS, Silliman K, Kirk LL. 1998. Commercial grape juices inhibit the in vitro oxidation of human low-density lipoprotein. J. Agric. Food Chem. 46(3): 834-836.
Glass CK, Witztum JL. 2001. Atherosclerosis the road ahead. Cell. 104(4): 503-516.
Goulinet S, Chapman MJ. 1993. Plasma lipoproteins in the Golden Syrian hamster (Mesocricetus auratus): heterogeneity of apoB- and apoA-I-containing particles. J Lipid Res. 34:943-959.
Grundy SM, Denke MA. 1990. Dietary influences on serum lipids and lipoproteins. J Lipid Res. 31(7): 1149-1172.
Grundy SM. 1997. Cholesterol and coronary heart diease. Arch Intern Med. 157(11): 1177-1184.
Heinecke JW. 1998. Oxidants and antioxidants in the pathogenesis of atherosclerosis: implication for the oxidized low density lipoprotein hypothesis. Atherosclerosis. 141(1): 1-15.
Hitunen TP, Luoma JS, Nikkari T, Y-H S. 1998. Expression of LDL receptor, VLDL receptor, LDL receptor-related protein, and Scavenger receptor in rabbit atherosclerotic lesions. Circulation. 97: 1079-1086.
Huang WC, Chen YM, Kan NW, Ho CS, Wei L, Chan CH, Huang HY, Huang CC. 2015. Hypolipidemic effects and safety of Lactobacillus Reuteri 263 in a hamster model of hyperlipidemia. Nutrients 7(5): 3767-3782.
Huang WC, Chen YM, Kan NW, Ho CS, Wei L, Chan H, Huang HU, Huang CC. 2015. Hypolipidemic effects and safety of Lactobacillus reuteri 263 in a hamster model of hyperlipidemia. Nutrients.7(5): 3767-3782.
Jamdar SC, Cao WF. 1992. Properties of monoglycerol acyltransferase in rat adipocytes. Arch Biochem Biophys. 296(2): 419-425.
Jasen MD, Haymond MW, Gerich JE, Cryer PE, Miles JM. 1987. Lipolysis during fasting. J Clin Invest. 79(1): 207-213.
Jialal I, Devaraj S. 1996. Low-density lipoprotein oxidation, antioxidants, and atherosclerosis: a clinical biochemistry perspective. Clin Chem. 42(4): 498-506.
Kleinveld HA, Hak-Lemmers HL, Stalenhoef AF, 1992. Demacker PN. Improved measurement of low-density-lipoprotein susceptibility to copper-induced oxidation: application of a short procedure for isolating low-density lipoprotein. Clin Chem. 38(10): 2066-2072.
Knekt P, Jarvinen R, Reunanen A, Maatela J. 1996. Flavonoid intake and coronary motality in Finland: a cohort study. Br. Med. J. 312(7029): 478-481.
Kowala MC, Nunnari JJ, Durham SK, NicolosiRJ. 1991. Doxazosin and cholestyramine similarly decrease fatty streak formation in the aortic arch of hyperlipidemic hamsters. Atherosclerosis. 91(1-2): 35-49.
Kragel AH, Reddy SG, Wittes JT, Roberts WC. 1989. Morphometric analysis of coronary arteries in acute myocardial infarction and in sudden coronary death. Circulation. 80(6): 1747-1756.
Krkoskova B, Mrazova Z. 2005. Prophylactic components of buckwheat. Food Res Intern. 38: 561-568.
Lusis AJ, Pajukanta P. 2008. A treasure trove for lipoprotein biology. Nat Genet. 40(2): 129-130.
Luskey KL. 1988. Regulation of cholesterol synthesis: mechanism for control of HMG-CoA reductase. Recent Prog Horm Res. 44: 35-51.
Mayakrishnana V, Kannappanb P, Abdullaha N and Ali-Ahmedc AB. 2013. Cardioprotective activity of polysaccharides derived from marine algae: An overview. Trends Food Sci Tech. 30(2): 98-104.
Mayse PA. 1988. Harper’s biochemistry, 21th eds, pp 241-252.
Mertens A, Holvoet P. 2001. Oxidized LDL and HDL: antagonists in atherothtombosis. FASEB J. 15(12): 2073-2084.
Narkprasoma k, Wangb SP, Hsiaoc SM, Tsaic PJ. 2012. Kinetics of color loss of djulis (Chenopodium formosanum koidz.) extracts during storage in different concentrations of alcohol and temperature. APCBEE Procedia. 2: 32-36.
Nistor A, Bulla A, Filip DA, Radu A. 1987. The hyperlipidemic hamster as a model of experimental atherosclerosis. Atherosclerosis. 68(1-2): 159-173.
Puhl H, Waeg G, Esterbauer H. 1994. Methods to determine oxidation of low-density lipoproteins. Methods Enzymol. 233: 425-441.
Raal FJ, Areias AJ, Waisberg R, von Arb M. 1995. Susceptibility of low density lipoprotein to oxidation in familial hypercholesterolaemia. Atherosclerosis. 115(1): 9-15.
Schaefer EJ. 1997. Effects of dietary fatty acids on lipoproteins and cardiovascular disease risk: summary. Am J Clin Nutr. 5: 1655-1656.
Schaffer JE, Lodish HF. 1994. Expression cloning and characterization of a novel adipocyte long chain fatty acid transport protein. Cell. 79(3): 427-436.
Seo JY, Kim SS, Kim JS. 2014. Enhancement of alcohol metabolism by sprouted peanut extract in SD rats. Prev. Nutr. Food Sci. 19(1): 1-4.
Shi Y, Guo R, Wang X, Yuan D, Zhang S, Wang J, Yan X, Wang C. 2014. The regulation of alfalfa saponin extract on key genes involved in hepatic cholesterol metabolism in hyperlipidemic rats. PLoS ONE. 9(2): e88282.
Smith CJ, Levy I, Sabin CA, Kaya E, Johnson MA, Lipman MCI. 2004. Cardiovascular disease risk factors and antiretroviral therapy in an HIV-positive UK population. HIV Med. 5(2): 88-92.
Stalenhoef AFH, Malloy MJ, Kane JP. 1984. Metabolism of apolipoprotein B-48 and B-100 of triglyceride-rich lipoproteins in normal and lipoprotein lipase-deficient humans. Proc Natl Acad Sci U S A. 81(6): 1939-1843.
Steinberg D, 1997. Oxidative modification of LDL and atherogenesis. Circulation. 95(4): 1062-1071.
Steinberg D. 1986. Studies on the mechanism of action of probucol. Am J Cardiol. 57:16H-21H.
Sullivan MP, Cerda JJ, Robbins FL, Burgin CW, Beatty RJ. 1993. The gerbil, hamster, and guinea pig as rodent models for hyperlipidemia. Lab Anim. 43(6): 575-578.
Thompson PD, Crouse SF, Goodpaster B, Kelley D, Moyna, Pescatello L. 2001. The acute versus the chronic response to exercise. Med Sci Sport Exer. 33: S438-S445.
Tsai PJ, Chen YS, Sheu CH, Chen CY. 2011. Effect of nanogrinding on the pigment and bioactivity of djulis (Chenopodium formosanum koidz.). J Agric Food Chem. 59(5): 1814-1820.
Tsai PJ, Sheu CH, Wu PH, Sun YF. 2010. Thermal and pH stability of beta-cyanin pigment of djulis (Chenopodium formosanum) in Taiwan and their relation to antioxidant activity. J Agric Food Chem. 58(2): 1020-1025.
Wilkinson J, Higgins JA, Fitzsimmons C, Bowyer DE. 1998. Dietary fish oils modify the assembly of VLDL and expression of the LDL receptor in rabbit liver. Arterioscler Thromb Vasc Biol. 18(9): 1490-1497.
Yoshida H, Kisugi R. 2010. Mechanisms of LDL oxidation. Clinica Chimica Acta. 411(23-24): 1875-1882.
Yusuf S, Ounpuu S. 2003. Tackling the growing global burden of atherosclerotic cardiovascular disease. Eur J. Cardiovasc Prev. Rehab. 10: 236-239.
Zhang Z, Wang H, Jiao R, Peng C, Wong YM, Yeung VS, HuangY, Chen ZY. 2009. Choosing hamsters but not rats as a model for studying plasma cholesterol-lowering activity of functional foods. Mol Nutr Food Res. 53(7): 921-930.
Zhu RG, Sun YD, Li TP, Chen G, Peng X, Duan WB, Zheng ZZ, a, Shi SL, Xu JG, Liu YH, Jin XY. 2015. Comparative effects of hawthorn (Crataegus pinnatifida Bunge) pectin and pectin hydrolyzates on the cholesterol homeostasis of hamsters fed high-cholesterol diets. Chem Biol Interact. 238: 42-47. 
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