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研究生:陳夙倩
研究生(外文):Chen, Su-Qian
論文名稱:有機肥料對仙草生長、產量與成分分析之研究
論文名稱(外文):Studies on the Organic Fertilizer for Growth, Yield, and Components Analysis in Hsian-tsao (Mesona chinensis Benth.)
指導教授:賴宏亮賴宏亮引用關係
指導教授(外文):Lay, Horng-Liang
口試委員:林資哲顏銘宏
口試委員(外文):Lin, Tzu-CheYen, Ming-Hong
口試日期:2017-06-28
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:農園生產系所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:中文
論文頁數:93
中文關鍵詞:仙草有機質肥料高效液相層析法
外文關鍵詞:Mesona chinensis Benth.organic fertilizerhigh performance liquid chromatography (HPLC)
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仙草 (Mesona chinensis Benth.)為唇形花科 (Lamiaceae)仙草屬(Mesona)一年生或越年生植物。富含凝膠,傳統採收地上部貯藏後製成消暑飲料及甜點食用,具保肝、預防糖尿病腎病變、抗氧化、抗突變和抗癌等功效。本研究施用植物性混合有機質肥料 (OF1)、植物性與動物性混合有機質肥料 (OF2)與有機質雞糞 (OF3)等三種有機肥,並與未施用有機質肥 (CK)進行對照,比較桃園1號 (TYU 1)及桃園2號 (TYU 2)生長盛期植株及TYU 2於不同採收期之生長、生產及七種成分之影響。探討最佳萃取條件並開發高效液相層析法 (High performance liquid chromatography, HPLC)進行定量分析。生產力調查結果顯示,土壤pH過高及其他氣候因素,引起生理障礙、養分逆境,因此三種有機肥料處理間無明顯的差異。以75% ethanol搭配超音波震盪萃取方法進行成分之分析,比較有機質肥料處理TYU 1及TYU 2比較兩品種生長盛期植株以及TYU 2於不同採收期植株之成分含量,結果顯示,以TYU 2含量較TYU 1高,且TYU 2以OF1處理可以提高protocatechuic acid、caffeic acid、rosmarinic acid及apigenin含量,分別為21.03±0.01 μg/g、56.59±1.00 μg/g、18,380.78±14.19 μg/g及1,015.80±9.83 μg/g。本論文研究結果可供作未來仙草栽培有機質肥料選擇、最佳採收適期及成分分析之參考。
Hsian-tsao (Mesona chinensis Benth.) is kind of annual or perennial herb plant, and belong to Mesona genus in Lamiaceae family. In general, harvested the leaf and stem, additionally increased gel content because of stored in several years. It has been consumed as a herbal drink and dessert. The main pharmatheutical activity effective against hepatic damage, diabetic nephropathy, anti-oxidant, anti-mutation and anticancer. In this study, applying three kinds of organic fertilizers, including plant residues (OF1), plant residues combined animal manures (OF2), chicken manure (OF3) and compared with no supplied organic fertilizer (CK). The effects of organic fertilizers treatment have compared Taoyuan No. 1 (TYU 1) with Taoyuan No. 2 (TYU 2) both varieties of M. chinensis Benth. at growth stage. Furthermore, TYU 2 has contrasted between monthly investigated of different harvest period. Studies on these treatment effect on growth, yield and seven components content. Studies also conducted on extraction conditions and quantitative analysis by high performance liquid chromatography (HPLC). The results indicated that the experimental soil above pH 7 or climatic conditions caused physiological and nutrient stress. Therefore, three organic fertilizers treatment are not significant difference. Extraction conditions, ultrasonic extraction method with 75% ethanol as extraction solvent. Analysis of components content of roots, stems and leave of growth stage TYU 1 and TYU 2, and the flowers. The results showed that TYU 2 is better than TYU 1 of components content. The treatment of OF1 increased the content of protocatechuic acid, caffeic acid, rosmarinic acid and apigenin were 21.03±0.01 μg/g、56.59±1.00 μg/g、18,380.78±14.19 μg/g及1,015.80±9.83 μg/g, respectively. The study could be the reference for cultivar and component analysis in the future.
摘要 I
Abstract II
謝誌 IV
目錄 V
圖表目錄 VII
壹、前言 1
貳、文獻回顧 3
一、仙草介紹 3
二、仙草形態特徵 3
三、仙草之栽培 4
四、仙草之採收與調製 4
五、仙草之生物活性與藥理活性 5
六、仙草成分分離與鑑定 7
七、仙草成分之藥理活性 9
參、材料與方法 12
一、研究架構 12
二、試驗材料 13
三、組織切片 15
四、田間試驗 18
五、有機質肥料處理仙草之定量分析 19
肆、結果 24
一、栽培環境氣象資料收集 24
二、組織切片 25
三、栽培土壤理化性質分析 31
四、有機質肥料對生長盛期TYU 1及TYU 2生長及產量之影響 33
五、有機質肥料處理下各月份TYU 2生長及產量之情形 39
六、有機質肥料處理對仙草成分之定量分析 49
伍、討論 78
一、組織鑑別 78
二、仙草生長及產量之探討 78
三、仙草成分分析之探討 79
陸、結論 81
參考文獻 82
作者簡介 93
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