臺灣博碩士論文加值系統

丹參 (Salvia miltiorrhiza Bung) 為唇形科 (Labiatae) 鼠尾草(Salvia) 的多年生藥用植物，使用部位為乾燥根部。主要活性成分以親脂性的二萜類 (diterpenoids) 與以及親水性的酚酸類 (phenolic acids) 為主。採後的乾處理是中草藥為了保存重要環節，但由於丹參的活性成分不穩定，易受溫度與外在環境影響而改變。因此本實驗使用冷凍乾燥、熱風乾燥、陰乾與曬乾等方式進行乾燥。並開發使用高效液相層析 (High performance liquid chromatography, HPLC) 之成分分析方法究探討不同的乾燥方式對於丹參根部中活性成分之影響。結果顯示酚酸類以冷凍乾燥處理的含量為最高分別為danshensu 744.89±51.89 μg/g、salvianolic acid B 13,534.37±417.42 μg/g及salvianolic acid A 193.69±8.78 μg/g，二萜類是以曬乾的含量為最高分別是dihydrotanshinone 120.50±4.72 μg/g、cryptotanshinone 657.86±34.77 μg/g、tanshinone I 240.24±22.04 μg/g及tanshinone IIA 600.66±40.31 μg/g，冷凍乾燥為次之。另外以不同溫度的熱風乾燥進行乾燥溫度試驗，分別以40℃、50℃、60℃與70℃來進行試驗，結果顯示酚酸類以50℃的烘乾有最高的含量分別是danshensu 503.70±25.41 μg/g、salvianolic acid B 17,110.78±466.40 μg/g及salvianolic acid A 166.58±11.00 μg/g親脂性的丹參酮類分別是50℃烘乾的dihydrotanshinone 97.08±2.72 μg/g以及70℃烘乾的cryptotanshinone 461.65±27.73 μg/g、tanshinone I 294.42±14.74 μg/g及tanshinone IIA 768.78±154.55 μg/g。50℃烘乾與70℃烘乾有最佳的含量。研究結果顯示由冷凍乾燥來進行乾燥的丹參中其的活性成分含量較高；如以烘乾進行乾燥則為50℃為最佳。

Danshen (Salvia miltiorrhiza Bunge), whose roots were often used as medicine, belongs to the family Labiatae and Salvia genus. The main active components are two categories that the lipophilic diterpenoids and the hydrophilic phenolic acids. Post-harvest drying is the important procedure in order to preserve Chinese herbal medicine. But the active ingredients of danshen is unstable, susceptible to temperature and external environmental changes. So the experiment is discussing the differences by HPLC (High performance liquid chromatography) component analysis method to explore the idifferent drying methods such as freeze-drying, hot air drying, shade drying and sun drying for the roots of danshen in the active ingredients of the impact. The results show the highest content of phenolic acids is in freeze-drying that danshensu is 744.89±51.89 μg/g, salvianolic acid B is 13,534.37±417.42 μg/g and salvianolic acid A is 387.39 ± 16.95 μg/g, The highest content of tanshinone is in sunburn that dihydrotanshinone is 120.50±4.72 μg/g, cryptotanshinone is 657.86±34.77 μg/g, tanshinone I 240.24±22.04 μg/g and tanshinone IIA is 600.66±40.31 μg/g, second is by freeze-drying. The drying test was carried out at 40℃, 50℃, 60℃ and 70℃, respectively. The results showed that the highest content of phenolic acids is at 50℃ that danshensu is 503.70±25.41 μg/g, salvianolic acid B is 17,110.78±466.40 μg/g and salvianolic acid A is 166.58±11.00 μg/g, The tanshinones were best at 50℃, dihydrotanshinone is 97.08±2.72 μg/g and 70℃ dried cryptotanshinone is 461.65±27.73 μg/g, tanshinone I is 294.42±14.74 μg/g and tanshinone IIA 768.78±154.55 μg/g. 50℃ drying and 70℃ have the best contents. The results showed that the content of active ingredients in S. miltiorrhiza was higher by freeze drying, and 50℃ was the best when dried by hot air drying.

摘 要 I
Abstract III
謝 誌 V
目 錄 VI
圖目錄 IX
表目錄 X
壹、前言 1
貳、文獻回顧 3
一、丹參介紹 3
二、丹參形態特徵 3
三、丹參之成分 4
(一) 丹參相關研究 4
(二) 萜類化合物 4
(三) 酚酸類化合物 5
四、丹參藥理活性 8
五、HPLC分析方法 10
(一) 親水性化合物 10
(二) 親酯性化合物 11
(三) 同時測定親水性與親酯性成分 12
六、丹參不同乾燥處理之成分比較 13
(一) 不同乾燥方式比較 13
(二) 不同乾燥溫度比較 13
參、材料與方法 15
一、實驗架構 15
二、試驗材料 16
(一) 材料來源 16
(二) 試藥與溶劑 16
(三) 儀器設備 18
三、丹參切片試驗 18
四、丹參定量分析試驗 22
(一) 檢品溶液製備 22
(二) 標準品溶液之製備 24
(三) 內部標準品溶液之製備 24
(四) 檢量線製作 25
(五) 丹參分析方法之確效試驗 25
(六) 高效液相層析法分析條件 27
(七) 資料統計分析 27
肆、結果 29
一、丹參品種鑑定 29
二、丹參成分之定量分析 34
(一) 成分分析 34
(二) 檢量線之分析 34
(三) 分析方法之確效試驗 35
三、丹參的產物定量分析試驗 41
(一) 最佳萃取條件 41
(二) 成分定量分析 42
伍、討論 54
一、兩品種丹參之基原鑑定 54
二、不同萃取條件之探討 54
(一) 不同萃取法之探討 55
(二) 不同萃取溶劑之探討 55
三、丹參成分之定量分析 56
(一) 不同粗細之丹參根 56
(二) 不同乾燥方式之影響 57
(三) 不同乾燥溫度之影響 58
陸、結論 60
參考文獻 62
作者簡介 73

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