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研究生:邱柏豪
研究生(外文):Po-hao Chiu
論文名稱:粗首鱲複合種群之粒線體基因族群遺傳研究
論文名稱(外文):Population genetics of Opsariichthys pachycephalus (Teleostei: Cyprinidae) species complex based on mitochondrial genes
指導教授:廖德裕
指導教授(外文):Te-Yu Liao
學位類別:碩士
校院名稱:國立中山大學
系所名稱:海洋科學系研究所
學門:自然科學學門
學類:海洋科學學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:中文
論文頁數:67
中文關鍵詞:粗首鱲複合種群親緣地理族群遺傳粒線體基因
外文關鍵詞:Opsariichthys pachycephalusPopulation geneticsMitochondrial DNA
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台灣的粗首鱲複合種群包含粗首鱲(Opsariichthys pachycephalus)及高屏鱲(O. kaopingensis)兩種。粗首鱲複合種群已有同功酶(isoenzyme)與粒線體基因D-loop之族群研究,發現台灣粗首鱲複合種群以曾文溪以北為一群,高屏溪以南為一群,但目前為止粗首鱲複合種群缺乏粒線體基因cytochrome b之研究,且過去研究其採樣溪流數量較少,所提供的資訊有限。因此本研究採集台灣西部22條河川之粗首鱲複合種群,共257個個體,定序粒線體cyt b及D-loop基因分析其族群遺傳結構。親緣關係樹及Nestwork analysis分析顯示粗首鱲複合種群分為兩大譜系,Lineage 1主要為曾文溪以北的溪流個體及枋山溪(含)以南恆春半島個體,再加上高屏溪及林邊溪各一個體。另一譜系(Lineage 2)為高屏溪、東港溪、林邊溪及士文溪。Lineage 1與Lineage 2的單型基因歧異度(H)為0.958及0.630,以及核苷酸歧異度(
The Opsariichthys pachycephalus species complex includes O.pachycephalus and O. kaopingensis. Previous studies of population genetics on O. pachycephalus species complex using isoenzyme and mitochondria DNA control region showed that this species complex were divided into two lineages, viz. north of Tengwen River and south of Kaoping River. In this study, two hundred and fifty-seven fishes of O.pachycephalus species complex from 22 rivers throughout the range of western Taiwan were collected. Population genetics structure of O. pachycephalus species complex were studied based on mitochondrial cytochrome b and control region sequences. The reconstructed phylogenetic tree indicates two distinct clades present in O. pachycephalus species complex, viz. Lineage 1 (from Lanyang River to Zengwen River, Fangshan River, Sizhong River, and Gangkoui River) and Lineage 2 (Kaoping River, Linbian River, Donggang River, and Shih Wen Creek). Relatively high levels of haplotype diversity (h=0.96) and low levels of nucleotide diversity (
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論文審定書…………………………………………………………………………….i
公開授權書…………………………………………………………………………....ii
謝辭…………………………………………………………………………………...iii
中文摘要……………………………………………………………………………...iv
英文摘要……………………………………………………………………………...vi
目錄………………………………………………………………………………….viii
表目錄…………………………………………………………………………….......ix
圖目錄………………………………………………………………………………....x
壹、 前言………………………………………………………………………………1
貳、 材料與方法………………………………………………………………………8
參、 結果……………………………………………………………………………..18
肆、 討論……………………………………………………………………………..24
伍、 結論……………………………………………………………………………..32
參考文獻……………………………………………………………………………..33
附表…………………………………………………………………………………..41
附圖…………………………………………………………………………………..50
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