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研究生:陳俊文
研究生(外文):CHEN, JYUN-WUN
論文名稱:莫三比克吳郭魚司坦尼氏降鈣素序列之分析
論文名稱(外文):Sequence Analysis of Stanniocalcin-1 in Tilapia(Oreochromis Mossambicus)
指導教授:曾登裕曾登裕引用關係
指導教授(外文):TSENG, DENG-YU
口試委員:林鳳嬌張德生
口試委員(外文):LIN, FENG-CHIAOCHANG, DE-SHENG
口試日期:2017-06-22
學位類別:碩士
校院名稱:國立臺南大學
系所名稱:生物科技學系碩士班
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:中文
論文頁數:59
中文關鍵詞:吳郭魚基因選殖司坦尼氏降鈣素鈣離子
外文關鍵詞:Oreochromis mossambicaGene cloningStanniocalcinCalcium
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生活在水中的魚類,必須面對滲透壓不平衡的問題,魚類透過鰓、皮膚、腎等器官其上的離子細胞及膜蛋白來運輸離子和水分,其中鈣離子是生物生理反應和骨頭的必需元素,魚利用鰓、小腸及腎臟自水中攝取鈣離子,而維生素 D、皮質醇(Cortisol)及降鈣素(Calcitonin)等荷爾蒙能調節鈣的吸收。司坦尼氏降鈣素(Stanniocalcin, STC)是一個大小約 50-kDa 的雙體醣蛋白,由附著在腎臟上的司坦尼氏小體(Corpuscle of Stannius, CS)所分泌,主要透過降低表皮鈣離子通道蛋白(Epithelial calcium channel, ECaC)表現量來抑制鈣離子吸收。

莫三比克吳郭魚(Oreochromis mossambica)對環境的離子變化具有很高的耐受性,是一種廣鹽性魚類。本實驗選殖到一條長度約 1,313 bp 的司坦尼氏降鈣素基因序列,可轉譯出 256 個胺基酸長度的蛋白質,此蛋白質序列與長吻雀鱔(Lepisosteus osseus)有 77 % 相似,弓鰭魚(Amia calva)有 75 % 相似,斑馬魚(Danio rerio)有 63 % 相似,家鼠(Mus musculus)有 54 % 相似,非洲爪蟾(Xenopus laevis)有 49 % 相似。基因表現方面,將孵化後仔魚經由皮質醇處理,進行定量聚合酶鏈鎖反應,測定表皮鈣離子通道和此基因 mRNA 的表現量,在第九小時兩者表現量有顯著上升,並確認此段基因的表現有所改變。實驗結果顯示此選殖序列為司坦尼氏降鈣素基因,並具有功能性的變化。

The regulation of osmotic pressure and ion are important for fish to live in freshwater or seawater. Some elements such as gill, skin, kidney and membrane protein are involved with transportation of ion and water. Calcium is an important component of a mineral necessary for all animals. It plays an important role in bone structure and biochemistry of organisms and the cell. One of the steroid hormones which can increase calcium concentration in blood is Cortisol. Stanniocalcin(STC)was originally identified in fish and suggested to participate in Ca2+ homeostasis. It’s a homodimeric glycoprotein that is secreted from the corpuscles of Stannius(CS), which are attached to the kidneys.

In the study, we find the 1313 base of overall nucleotide sequence of stanniocalcin in tilapia(Oreochromis mossambica)by gene cloning. The expression of ECaC and STC-1 during tilapia larvae development in environment of additional cortisol is obviously increased in 9 hours sample. The relation between ECaC and STC-1 in tilapia will be the purpose.

目次
摘要 2
Abstract 3
目錄 5
前言 6
材料與方法 15
結果 28
討論 31
參考文獻 37
表 48
圖 51
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