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研究生:黃筱粧
研究生(外文):Huang, Xiao-Zhuang
論文名稱:應用氣相層析儀/哨子技術探討酸性溶液對種子萌發及呼吸作用的影響
論文名稱(外文):The use of a gas chromatography/Milli-whistle technique for the on-line monitoring simulated acid solution effect on Mung bean germination and respiration
指導教授:林震煌
學位類別:碩士
校院名稱:國立臺灣師範大學
系所名稱:化學系
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:中文
論文頁數:66
中文關鍵詞:微型發音哨氣相層析儀LabVIEW呼吸作用種子萌發
外文關鍵詞:milli-whistlegas chromatographyseed respirationLabVIEW
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本研究以氣相層析/聲波感測方式,長時間對綠豆種子發芽過程中的呼吸商 (每分鐘氧消耗量和二氧化碳產生量之比;Respiratory Quotient)進行即時偵 測。藉由計算呼吸作用反應的速率常數與溫度之間的關係式,依照 Arrhenius 方程式作圖,成功獲得不同溫度及不同 pH 之下的反應活化能。 氣相層析/聲波感測法是利用串接在氣相層析管柱尾端的微哨感測器,當種子 發芽過程產生的氧氣與二氧化碳,經氣相層析管柱分離後,會在哨子端產生不 同頻率的聲波。經快速傅立葉轉換後,可以得到頻率即時的層析圖譜。呼吸商則可以用來了解各種營養基底被利用的比例。呼吸商為 1 時, 能量主要由碳水化合物供給。本實驗先配製不同p值的溶液(pH 值:3~7),在暗室中進行不同溫度(溫 度:25~30 度)的綠豆呼吸作用反應,並同步長時間測量呼吸商的數值(每 5 分 鐘測量一次,連續 12 小時的即時線上記錄監測)。實驗結果發現,酸性越強的溶液中,種子的呼吸速率也隨之下降。在中性的環境下(pH, 7.0),萌芽前 的 5 個小時內,呼吸商的數值都非常接近 1。這表示此階段主要是以碳水化合物作為呼吸作用的養份來源。而隨後的 8 個小時內,呼吸商逐漸降至 0.6以下。這表示養份的利用將逐漸轉成為其他成分。此外,種子在酸性溶液中,碳水化合物的利用容易受到抑制。但是,如果將
II
溫度增加的話,葡萄糖被抑制使用的情況可以得到舒減。由上述的實驗結果,依照 Arrhenius 方程式作圖,可以得到不同條件下的反應活化能。在溶液的 pH 值為 3, 4, 5, 6, 7 的情況下,反應 活化能分別為17.5834,17.0047,16.2955,11.5170,11.2704 kJ mol-1。尤其當 pH=3 時,活化能為 17.5834,明顯大於其他 pH 值之下的活化能,清楚說明了強酸的情況下需要較大活化能才能維持種子萌發的生理機能。 利用本實驗裝置可以即時分析種子發芽時所需要的最佳 pH 環境及最佳溫度。本研究所開發的裝置,非常容易用來探知呼吸最緩慢的條件。日後可應用在蔬 果食品保存或魚鮮肉類輸送過程,所需節能省碳等的最佳條件。
In this study, the dynamic response of seed germination and respiration of Mung bean to simulated acid rain stress were investigated. The sterilized seeds were dipped in simulated acid rain solution with pH 7, 6, 5 ,4 ,3 for 12 hours and temperature effect was also considered. To evaluate the respiration rate during the seed germination, a gas chromatography/milli-whistle device was used to on-line monitor the production of oxygen and carbon dioxide. The milli-whistle was connected to the outlet of a GC capillary, and when the analyte gases and the GC carrier gas pass through it, a sound with a fundamental frequency is produced. The online data obtained for frequency vs. retention time can be recorded after a fast Fourier transform. With the decrement of pH of acid rain solution, the respiration rate of Mung bean was decreased, The effect of simulated acid rain on the germinated energy in pH value 7 to 3 the activated energy was 17.5834kJ mol-1, 17.0047kJ mol-1, 16.2955kJ mol-1, 11.5170kJ mol-1 and 11.2704kJ mol-1 respectively. Simulation of acid rain could restrain the germination of seed in some degree and the restraining effect strengthened with the rise of acid rain degree.
中文摘要 I
Abstract III
目錄 IV
圖目錄 VII
表目錄 X
第一章 緒論 1
1-1 研究目的 1
1-2 酸性環境的植物萌發 2
第二章 研究方法及原理 3
2-1 微哨聲波感測器 3
2-1-1 微哨感測器的偵測原理 4
2-1-2 微哨感測器的開發設計 8
2-1-3 偵測極限與儀器改良 10
2-2 種子的萌發 16
2-2-1 呼吸作用 16
2-2-2呼吸作用指標 19
2-2-3影響呼吸作用的因素 22
2-3 生物活化能的理論計算 23
第三章 儀器、藥品與實驗方法 25
3-1 實驗儀器 25
3-1-1微哨感測器 27
3-1-2氣相層析儀 28
3-1-3自組裝恆溫槽 29
3-1-4自組裝自動進樣裝置 30
3-2 裝置控制、資料處理與LabVIEW編程 32
3-3 儀器設備與藥品列表 36
第四章 研究過程與結果討論 39
4-1 酸性溶液配置 39
4-2種子處理 39
4-3 種子萌發初期的反應條件與操作 39
4-4氧氣、二氧化碳檢量線 39
4-5 與市售氣體感測器之比較 42
4-6 呼吸作用反應過程 45
4-7 種子在不同酸性條件下的生理表現 47
4-7-1呼吸速率的測定 47
4-7-2 種子萌發率 49
4-7-3 呼吸商隨時間變化 50
4-8 呼吸作用的活化能計算 53
4-9 其他氣體之偵測應用 57
4-9-1 GC/哨子應用於即時偵測金屬錯合物氧氣催化反應 57
4-9-2 測定人體呼氣中氧氣/二氧化碳比與血糖的關係 59
第五章 結論 62
參考資料 63
學會發表 66
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