臺灣博碩士論文加值系統

本論文以水解魚蛋白(FPH)、水解大豆蛋白(SPH)、水解大豆蛋白與大豆粉1:1(w:w)混合物(SPHSB)探討飼料中取代不同魚粉蛋白比例對金目鱸的成長及免疫反應影響。
實驗一：以FPH分別取代魚粉蛋白0% (FPH0)、5% (FPH5)、10% (FPH10)、15% (FPH15)、20% (FPH20)之飼料，投餵初重0.91g ± 0.01g的金目鱸，實驗進行6週。飼料粗蛋白、粗油脂分別為50.12-51.59%、10.93-11.79%。金目鱸增重率以餵食飼料FPH5組有最高增重率，與控制組、FPH10、FPH20組無顯著差異，顯著高於FPH15組。O2–產生率在FPH5組顯著高於控制組、FPH10、FPH15、FPH20組；FPH20組有最低O2–產生率，顯著低於控制組、FPH5、FPH10組，與FPH15組無顯著差異。SOD活性各組無顯著差異。
實驗二：以SPH分別取代魚粉蛋白0% (SPH0)、10% (SPH10)、20% (SPH20)、30% (SPH30)、40% (SPH40)、50% (SPH50)之飼料，投餵初重0.43g ± 0.01g金目鱸，實驗進行6週。飼料粗蛋白、粗油脂分別為47.50-48.17%、14.82-15.29%。金目鱸最佳增重率為餵食飼料SPH30組，顯著高於控制組、SPH10、SPH20、SPH40、SPH50組，與SPH20組無顯著差異；SPH10、SPH20、SPH40、SPH50則與控制組無顯著差異；而O2–產生率部分控制組最高，與SPH10、SPH20和SPH30組無顯著差異，SPH40和SPH50組顯著低於SPH10和控制組；而SPH20、SPH30、SPH40、SPH50組之間無顯著差異。SOD活性各組皆無顯著差異。
實驗三：以SPHSB分別取代魚粉蛋白0%(SPHSB0)、10%(SPHSB10)、20%(SPHSB20)、30%(SPHSB30)、40%(SPHSB40)、50%(SPHSB50)之飼料，投餵初重0.36g ± 0.00g金目鱸，實驗進行6週。飼料粗蛋白、粗油脂分別為44.88-45.97%、11.17-12.78%。金目鱸最佳增重率為餵食飼料SPHSB10組，顯著高於SPHSB30、SPHSB40、SPHSB50組，與控制組、SPHSB20組無顯著差異；SPHSB40、SPHSB50組顯著低於SPHSB10、SPHSB20、SPHSB30和控制組；O2–產生率在SPHSB20組最高，與SPHSB30、SPHSB40組無顯著差異，顯著高於控制組、SPHSB10、SPHSB50組；而SPHSB10、SPHSB30、SPHSB40、SPHSB50組與控制組無顯著差異。SOD活性各組間無顯著差異。

This study investigated the effects of replacement of fishmeal protein with fish protein hydrolysate (FPH), soybean protein hydrolysate (SPH), soybean protein hydrolysate and soybean meal mixture (SPHSB) on the growth performance and immune parameters of Asian seabass, Lates calcarifer.
In experiment I, 0.91 g fish were fed five isonitrogenous (50%) and isolipidic (11%) diets containing FPH to replace 0% (FPH0), 5% (FPH5), 10% (FPH10), 15% (FPH15) and 20% (FPH20) of fish meal protein for 6 weeks. The triplicate groups of fish were fed three times a day ad lib. The weight gain of fish fed FPH5 was significantly higher than that of fish fed FPH15. The weight gain of fish fed FPH5 had no significant differences from that of fish fed FPH0, FPH10 and FPH20. The fish fed FPH5 had significantly higher superoxide anion (O2 -) production ratio than those fed other treatments. The SOD activity of fish showed no significant difference among treatments.
In experiment II, 0.43 g fish were fed six isonitrogenous (48%) and isolipidic (15%) diets containing SPH to replace 0% (SPH0), 10% (SPH10), 20% (SPH20), 30% (SPH30), 40% (SPH40) and 50% (SPH50) of fish meal protein for 6 weeks. The triplicate groups of fish were fed three times a day ad lib. The weight gain of fish fed SPH30 was significantly higher than that of fish fed other treatments except SPH20. The weight gain of fish fed SPH0 had no significant difference from that of fish fed SPH10, SPH20, SPH40 and SPH50. The fish fed SPH40 and SPH50 had significantly lower superoxide anion (O2 -) production ratio than those fed other treatments. The SOD activity showed no significant differences among treatments.
In experiment III, 0.36 g fish were fed six isonitrogenous (45%) and isolipidic (12%) diets containing SPHSB to replace 0% (SPHSB0), 10% (SPHSB10), 20% (SPHSB20), 30% (SPHSB30), 40% (SPHSB40) and 50% (SPHSB50) of fish meal protein for 6 weeks. The triplicate groups of fish were fed three times a day ad lib. The weight gain of fish fed SPHSB10 was significantly higher than that of fish fed SPHSB30, SPHSB40 and SPHSB50. The weight gain of fish fed SPHSB10 had no significant differences from that of fish fed SPHSB0 and SPHSB20. The fish fed SPHSB20 had significantly higher superoxide anion (O2 -) production ratio than those fed SPHSB0, SPHSB10, SPHSB50.The superoxide anion (O2 -) production ratio of fish fed SPHSB20 showed no significant difference from that of fish fed SPHSB30 and SPHSB40.The SOD activity of fish showed no significant difference among treatments.

謝辭......................................................I
摘要.....................................................II
Abstract................................................III
List of Tables...........................................VI
前言......................................................1
文獻整理..................................................2
1.金目鱸分類及介紹.........................................2
2.蛋白質..................................................5
3.蛋白水解物..............................................6
4.魚類非特異免疫..........................................9
5.水解物作為免疫刺激物應用.................................10
材料方法..................................................11
一、實驗動物..............................................11
二、實驗設計..............................................11
三、實驗飼料..............................................12
四、魚體採樣及收集.........................................12
五、粗成份分析............................................13
六、免疫分析..............................................14
七、成長參數..............................................15
八、統計分析..............................................15
結果.....................................................16
討論.....................................................19
結論.....................................................24
參考文獻..................................................25

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