臺灣博碩士論文加值系統

細菌纖維素因其特殊的奈米纖維結構使其具有高孔隙度與高孔洞表面積，又因纖維素具親水性，因此具有高含水量 (water holding capacity, WHC)的特性，本實驗利用Chen (2015) 所提出之瀝乾法測量細菌纖維素之含水量，並將測量裝置加以改良。
實驗定義瀝乾時間間隔：∆T為水滴落下的時間間隔，當實驗滿足水滴持續∆T min不再落下則瀝乾實驗結束。本研究確立一瀝乾準則：瀝乾時間間隔 ∆T = 6 min, 纖維素面積A = 16 cm2，在此準則下測量細菌纖維素的含水量，其實驗結果能客觀地代表此纖維素的含水量。此時細菌纖維素含水量變異係數 (coefficient of variation)小於5 %，瀝乾時間不超過30 min且實驗過程中蒸發損失小於 1.5 %。
本研究利用已確立之瀝乾準則測量甘油/纖維素膜含有甘油水溶液之能力 (glycerol holding capacity, GHC)，實驗發現隨著甘油濃度增加，甘油/纖維素膜含有溶液的孔洞體積會略微增加，纖維素逐漸膨潤，導致GHC隨之增加。
利用瀝乾法測量熱風乾燥後之甘油/纖維素膜之復水量 (water rehydration capability, WRC)。實驗發現當甘油濃度大於10 wt %時，熱風乾燥後殘留在纖維素結構內的甘油能避免纖維素結構被破壞，使纖維素之復水量提升到未乾燥纖維素含水量的90 %以上。
熱風乾燥後之甘油/纖維素膜，其復水後之孔隙度與通透性相對於熱風乾燥纖維素有明顯的提升，孔隙度由96 %以下提升至 99.3 %以上，而其通透性由2*10-6 ~3*10-6 cm2/sec提升到6*10-6 ~6.5*10-6 cm2/sec，但兩者皆略小於未乾燥之纖維素。

Bacterial Cellulose (BC) of unique nano-fibers structure and strong hydrophilicity is noted for its high water holding capacity (WHC). In this study WHC was measured by a draining method proposed by Chen (2015) with a slight modification.
Experimental results suggested that a convenient set of draining criteria: time interval between draining droplets (∆T) = 6 min and the size of BC (A) = 16 cm2 to measure. WHC would satisfactorily result in less than 5 % coefficient of variation, and evaporation loss is less than 1.5 % within 30 mins.
Furthermore, GHC (glycerol holding capacity) of gly/BC membrane was measured via this draining method by soaking BC memebrane in glycerol solution of various concentrations. The results showed that as the concentration of glycerol solution increased, GHC of gly/BC increased. Meanwhile, as the concentration of glycerol solution increased, up to 12 % swelling effect was noted.
As to WRC (water rehydration capability) of BC dried by hot air, the experimental results showed that the WRC of gly/BC (glycerol concentration 10 wt % or more) could retain at least 90 % of capability compared to only 5 % by hot-air dried BC and 20~40% by freeze-dried BC.
The porosity of rehydrated gly/BC was higher than 99.3 % and the permeability was about 6*10-6 ~6.5*10-6 cm2/sec, which were slightly lower than never-dried BC, but far greater than the hot-air dried BC and freeze-dried BC.

謝辭 I
摘要 II
Abstract III
目錄 IV
表目錄 VII
圖目錄 IX
第一章 緒論 1
第二章 文獻回顧 2
2.1 細菌纖維素生產菌株 2
2.1.1 Acetobacter xylinum 4
2.1.2 細菌纖維素的合成機制 7
2.2 細菌纖維素 10
2.2.1 細菌纖維素的特性 11
2.3 細菌纖維素之含水量 16
2.3.1 纖維素與水之交互作用 16
2.3.2 測量細菌纖維素含水量之方法 19
2.4 細菌纖維素之修飾與含水量 25
2.4.1 原位修飾 (In situ modification) 26
2.4.2 異位修飾 (Ex situ modification) 29
2.5 細菌纖維素之應用 32
2.5.1 細菌纖維素應用於傷口敷料 33
2.5.2 細菌纖維素應用於面膜 36
2.6 細菌纖維素之通透性 38
2.6.1 通透性 (permeability)之計算 40
第三章 實驗方法 45
3.1 實驗菌株 45
3.2 培養基組成 46
3.3 實驗方法 49
3.3.1 細菌纖維素 (bacterial cellulose, BC) 49
3.3.2 細菌纖維素含水量之測量 51
3.3.3 細菌纖維素復水量之測量 52
3.3.4 細菌纖維素通透性之測量 54
3.3.5 細菌纖維素孔隙度之測量 55
3.3.6 甘油濃度之定量 57
3.3.7 葡萄糖濃度之定量 60
3.4 實驗藥品 63
3.5 實驗儀器 64
第四章 實驗結果與討論 65
4.1 瀝乾法測量裝置 65
4.1.1 測量裝置設計 65
4.1.2 測量環境設計 68
4.1.3 細菌纖維素濕重之讀取 70
4.1.4 瀝乾時間間隔 (∆T) 71
4.1.5 細菌纖維素在尼龍網上之擺放方式 72
4.2 確立瀝乾準則 79
4.2.1 瀝乾時間間隔與含水量之關係 79
4.2.2 含水量變異係數 (coefficient of variation) 83
4.2.3 落下水滴重對含水量改變量之影響 88
4.2.4 瀝乾時間 (t) 90
4.2.5 蒸發損失 93
4.2.6 瀝乾準則之確立 96
4.2.7 細菌纖維素厚度與含水量之關係 98
4.3 甘油/纖維素膜含有甘油溶液之能力 102
4.3.1 甘油/纖維素膜含有甘油溶液之能力 103
4.3.2 甘油/纖維素膜之孔洞體積 107
4.3.3 甘油/纖維素膜之吸濕性 112
4.4 細菌纖維素復水量 118
4.4.1 細菌纖維素復水量 119
4.4.2 甘油/纖維素膜熱風乾燥後之甘油含量 124
4.4.3 細菌纖維素復水分率之比較 130
4.5 細菌纖維素復水後之通透性 133
4.5.1 熱風乾燥纖維素之通透性 134
4.5.2 熱風乾燥甘油/纖維素膜之通透性 137
4.5.3 含水量、復水量與通透性之比較 143
4.6 細菌纖維素復水後之孔隙度 146
第五章 結論 150
參考文獻 153

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