臺灣博碩士論文加值系統

白點症病毒(white spot syndrome virus, WSSV)主要以海洋甲殼類為宿主，並且對對蝦類有極高的致死率，自1992年蝦白點症爆發以來，造成水產養殖業的經濟損失，然而目前仍缺乏有效的治療方式。白點症病毒於感染宿主後首先藉由宿主的轉錄因子協助表現其極早期基因，這些基因包含病毒的轉錄調控因子，因此在病毒的生活史中是連結宿主與病毒調控機制的樞紐。極早期蛋白質WSSV108、WSSV126 (IE1)、WSSV136及WSSV156曾被報導具有轉錄調節活性，但目前對於白點症病毒自身基因的轉錄調控機制仍了解不多。本研究室先前研究發現白點症病毒極早期蛋白質WSSV108能活化wssv304及其他的病毒基因，驗證了WSSV108在細胞中具有調控轉錄的能力，而本研究透過免疫螢光染色發現WSSV108會聚集於細胞核中，確實可能參與轉錄作用。本研究也透過GST pull-down及冷光報導分析，發現WSSV108可調控其自身的啟動子，也會與白蝦(Litopenaeus vannamei)轉錄因子LvYY1的N端區域結合，並共同促進wssv108啟動子的轉錄，顯示wssv108的表現存在正回饋的機制，利於病毒快速表現WSSV108並促進其下游基因表現。而透過RNA干擾抑制病毒wssv108表現則導致病毒複製能力大幅減弱，並顯著提升了宿主受病毒感染後的存活率，顯示WSSV108對於白點症病毒是複製與致病過程中重要的蛋白質。總結上述結果，本研究發現WSSV108在白點症病毒的生活史扮演重要的角色，可能透過與宿主的LvYY1共同調控病毒的基因表現。

摘要 i
Abstract ii
目錄 v
1. 前言 1
1.1 白點症(White spot syndrome)的起源與影響 1
1.2 白點症病毒的基本特性 2
1.3 白點症的防治 2
1.4 白點症病毒生活史 4
1.5 白點症病毒的極早期基因 5
1.6 白點症病毒極早期基因wssv108 6
1.7 Yin Yang 1 (YY1)轉錄因子 7
研究目的 10
2. 材料與方法 11
2.1 菌種、細胞株、實驗動物及病毒 11
2.2 質體建構 11
2.3 質體DNA的萃取 11
2.4 暫行性細胞轉染 11
2.5 重組蛋白質的誘導表現 12
2.6 蛋白質的純化 12
2.7 抗體的純化 13
2.8 免疫沉澱法(Immunoprecipitation) 14
2.9 免疫共沉澱法(Co-immunoprecipitation) 14
2.10 西方墨點法(Western blotting) 14
2.11 冷光報導分析(Luciferase reporter assay) 15
2.12 GST pull-down 15
2.13 免疫螢光染色法(Immunofluorescence analysis) 15
2.14 雙股RNA的製備 16
2.15 雙股RNA與病毒注射 16
2.16 組織RNA的萃取與反轉錄 17
2.17 組織DNA的萃取與PCR 17
3. 結果 18
3.1 抗WSSV108抗體的製備及純化 18
3.2 WSSV108在受白點症病毒感染的白蝦中的表現 19
3.3 WSSV108於細胞中的分布 19
3.4 WSSV108與LvYY1對wssv108啟動子的調控 20
3.5 WSSV108對白點症病毒感染蝦宿主的重要性 21
4. 討論 24
5. 圖表 31
表1、引子列表 33
表2、質體列表 31
圖1、WSSV108在大腸桿菌E. coli BL21(DE3)的表現 34
圖2、抗WSSV108抗體效力的測試 35
圖3、WSSV108於受感染宿主中的表現 36
圖4、WSSV108入核序列的線上分析 37
圖5、WSSV108在昆蟲細胞Sf21中的分布 38
圖6、LvYY1與WSSV108的結合區域分析 39
圖7、WSSV108與LvYY1在細胞中的結合 40
圖8、在Sf21細胞中WSSV108和LvYY1對wssv108啟動子的影響 41
圖9、利用雙股RNA抑制WSSV108的表現 42
圖10、抑制wssv108的表現對WSSV複製的影響 43
圖11、抑制wssv108於WSSV感染時的表現對宿主的影響 44
參考文獻 45
附錄 61
圖S1、His-WSSV108抗原的純化 61
圖S 2、抗WSSV108抗體的純化 62
圖S 3、以大腸桿菌表現WSSV108及其部分片段 63
圖S 4、在Sf21細胞中WSSV108和LvYY1對ie1啟動子的影響 64
圖S 5、在S2細胞中WSSV108和LvYY1對wssv108及ie1啟動子的影響 65

Austen, M., B. Luscher & J. M. Luscher-Firzlaff (1997) Characterization of the transcriptional regulator YY1. The bipartite transactivation domain is independent of interaction with the TATA box-binding protein, transcription factor IIB, TAFII55, or cAMP-responsive element-binding protein (CPB)-binding protein. J Biol Chem, 272, 1709-17.
Balasubramanian, G., M. Sarathi, C. Venkatesan, J. Thomas & A. S. Sahul Hameed (2008) Oral administration of antiviral plant extract of Cynodon dactylon on a large scale production against White spot syndrome virus (WSSV) in Penaeus monodon. Aquaculture, 279, 2-5.
Bernhard, W., K. Barreto, S. Raithatha & I. Sadowski (2013) An upstream YY1 binding site on the HIV-1 LTRcontributes to latent infection. PLoS One, 8.
Bertani, G. (1951) Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J Bacteriol, 62, 293-300.
Bhalla, S. S., L. Robitaille & M. Nemer (2001) Cooperative activation by GATA-4 and YY1 of the cardiac B-type natriuretic peptide promoter. J Biol Chem, 276, 11439-45.
Bonnet, M. C., R. Weil, E. Dam, A. G. Hovanessian & E. F. Meurs (2000) PKR Stimulates NF-κB Irrespective of Its Kinase Function by Interacting with the IκB Kinase Complex. Mol Cell Biol, 20, 4532-42.
Brameier, M., A. Krings & R. M. MacCallum (2007) NucPred--predicting nuclear localization of proteins. Bioinformatics, 23, 1159-60.
Chang, L.-K., P.-H. Huang, W.-T. Shen, S.-H. Yang, W.-J. Liu & C.-F. Lo (2012) Role of Penaeus monodon Kruppel-like factor (PmKLF) in infection by white spot syndrome virus. Developmental & Comparative Immunology, 36, 121-129.
Chang, L. K., Y. H. Lee, T. S. Cheng, Y. R. Hong, P. J. Lu, J. J. Wang, W. H. Wang, C. W. Kuo, S. S. Li & S. T. Liu (2004) Post-translational modification of Rta of Epstein-Barr virus by SUMO-1. J Biol Chem, 279, 38803-12.
Chang, P.-S., H.-C. Chen & Y.-C. Wang (1998) Detection of white spot syndrome associated baculovirus in experimentally infected wild shrimp, crab and lobsters by in situ hybridization. Aquaculture, 164, 233-242.
Chang, P. J., Y. S. Chang & S. T. Liu (1998) Role of Rta in the Translation of Bicistronic BZLF1 of Epstein-Barr Virus. J Virol, 72, 5128-36.
Chang, Y. S., C. F. Lo, S. E. Peng, K. F. Liu, C. H. Wang & G. H. Kou (2002) White spot syndrome virus (WSSV) PCR-positive Artemia cysts yield PCR-negative nauplii that fail to transmit WSSV when fed to shrimp postlarvae. Dis Aquat Organ, 49, 1-10.
Chen, A. J., L. Gao, X. W. Wang, X. F. Zhao & J. X. Wang (2013) SUMO-conjugating enzyme E2 UBC9 mediates viral immediate-early protein SUMOylation in crayfish to facilitate reproduction of white spot syndrome virus. J Virol, 87, 636-47.
Chen, K. Y., T. C. Hsu, P. Y. Huang, S. T. Kang, C. F. Lo, W. P. Huang & L. L. Chen (2009) Penaeus monodon chitin-binding protein (PmCBP) is involved in white spot syndrome virus (WSSV) infection. Fish Shellfish Immunol, 27, 460-5.
Chen, L. L., H. C. Wang, C. J. Huang, S. E. Peng, Y. G. Chen, S. J. Lin, W. Y. Chen, C. F. Dai, H. T. Yu, C. H. Wang, C. F. Lo & G. H. Kou (2002) Transcriptional analysis of the DNA polymerase gene of shrimp white spot syndrome virus. Virology, 301, 136-47.
Cheng-Ming, C. & R. G. Roeder (1995) Cloning of an intrinsic human TFIID subunit that interacts with multiple transcriptional activators. Science, 267, 531.
Chou Hy, H. C. Y., C. H. Wang, H. C. Chiang & C. F. Lo (1995) Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan. Diseases of Aquatic Organisms, 23, 165-173.
Chou, H. Y., C. Y. Huang, C. F. Lo & G. H. Kou (1998) Studies on transmission of white spot syndrome associated baculovirus (WSBV) in Penaeus monodon and P. japonicus via waterborne contact and oral ingestion. Aquaculture, 164, 263-276.
Cock, J., T. Gitterle, M. Salazar & M. Rye (2009) Breeding for disease resistance of Penaeid shrimps. Aquaculture, 286, 1-11.
Durand, S., D. V. Lightner, L. M. Nunan, R. M. Redman, J. Mari & J. R. Bonami (1996) Application of gene probes as diagnostic tools for White Spot Baculovirus (WSBV) of penaeid shrimp. Diseases of Aquatic Organisms, 27, 59-66.
Flegel, T. W. (2006) Detection of major penaeid shrimp viruses in Asia, a historical perspective with emphasis on Thailand. Aquaculture, 258, 1-33.
Grodzki, A. C. & E. Berenstein (2010) Antibody purification: affinity chromatography - protein A and protein G Sepharose. Methods Mol Biol, 588, 33-41.
Guan, Y., Q. Zhu, D. Huang, S. Zhao, L. J. Lo & J. Peng (2015) An equation to estimate the difference between theoretically predicted and SDS PAGE-displayed molecular weights for an acidic peptide. Scientific reports, 5, 13370.
Hansen, L. H., S. Knudsen & S. J. Sørensen (1998) The effect of the lacY gene on the induction of IPTG inducible promoters, studied in Escherichia coli and Pseudomonas fluorescens. Current Microbiology, 36, 341-347.
Harlow, E. & D. Lane (1988) A laboratory manual. New York: Cold Spring Harbor Laboratory, 579.
He, F. & J. Kwang (2008) Identification and characterization of a new E3 ubiquitin ligase in white spot syndrome virus involved in virus latency. Virol J, 5, 151.
Huang, J., F. Li, J. Wu & F. Yang (2015) White spot syndrome virus enters crayfish hematopoietic tissue cells via clathrin-mediated endocytosis. Virology, 486, 35-43.
Huang, P. H., T. Y. Huang, P. S. Cai & L. K. Chang (2017) Role of Litopenaeus vannamei Yin Yang 1 in the regulation of the white spot syndrome virus immediate-early gene ie1. J Virol, 91.
Huang, P. H., S. C. Lu, S. H. Yang, P. S. Cai, C. F. Lo & L. K. Chang (2014) Regulation of the immediate-early genes of white spot syndrome virus by Litopenaeus vannamei kruppel-like factor (LvKLF). Dev Comp Immunol, 46, 364-72.
Huang, X.-D., L. Zhao, H.-Q. Zhang, X.-P. Xu, X.-T. Jia, Y.-H. Chen, P.-H. Wang, S.-P. Weng, X.-Q. Yu, Z.-X. Yin & J.-G. He (2010) Shrimp NF-κB binds to the immediate-early gene ie1 promoter of white spot syndrome virus and upregulates its activity. Virology, 406, 176-180.
Huang, Z. J., S. T. Kang, J. H. Leu & L. L. Chen (2013) Endocytic pathway is indicated for white spot syndrome virus (WSSV) entry in shrimp. Fish Shellfish Immunol, 35, 707-15.
Itami, T., M. Asano, K. Tokushige, K. Kubono, A. Nakagawa, N. Takeno, H. Nishimura, M. Maeda, M. Kondo & Y. Takahashi (1998) Enhancement of disease resistance of kuruma shrimp, Penaeus japonicus, after oral administration of peptidoglycan derived from Bifidobacterium thermophilum. Aquaculture, 164, 277-288.
Jaree, P., S. Senapin, I. Hirono, C.-F. Lo, A. Tassanakajon & K. Somboonwiwat (2016) WSV399, a viral tegument protein, interacts with the shrimp protein PmVRP15 to facilitate viral trafficking and assembly. Developmental & Comparative Immunology, 59, 177-185.
Kang, S. T., H. C. Wang, Y. T. Yang, G. H. Kou & C. F. Lo (2013) The DNA virus white spot syndrome virus uses an internal ribosome entry site for translation of the highly expressed nonstructural protein ICP35. J Virol, 87, 13263-78.
Kavran, J. M. & D. J. Leahy (2014) Coupling cntibody to Cyanogen Bromide-activated sepharose. Methods Enzymol, 541, 27-34.
Khalil, M. I., M. Sommer, A. Arvin, J. Hay & W. T. Ruyechan (2014) Cellular transcription factor YY1 mediates the varicella-zoster virus (VZV) IE62 transcriptional activation. Virology, 449, 244-53.
Kim, C. S., Z. Kosuke, Y. K. Nam, S. K. Kim & K. H. Kim (2007) Protection of shrimp (Penaeus chinensis) against white spot syndrome virus (WSSV) challenge by double-stranded RNA. Fish & Shellfish Immunology, 23, 242-246.
Kleiman, E., H. Jia, S. Loguercio, A. I. Su & A. J. Feeney (2016) YY1 plays an essential role at all stages of B-cell differentiation. Proc Natl Acad Sci U S A, 113, E3911-20.
Kono, T., R. Savan, M. Sakai & T. Itami (2004) Detection of white spot syndrome virus in shrimp by loop-mediated isothermal amplification. Journal of Virological Methods, 115, 59-65.
Kou, G.-H., S.-E. Peng, Y.-L. Chiu & C.-F. Lo (1998) Tissue distribution of white spot syndrome virus (WSSV) in shrimp and crabs. Advances in shrimp biotechnology, 267-271.
Lan, Y., W. Lu & X. Xu (2002) Genomic instability of prawn white spot bacilliform virus (WSBV) and its association to virus virulence. Virus Research, 90, 269-274.
Lee, J. S., K. M. Galvin, R. H. See, R. Eckner, D. Livingston, E. Moran & Y. Shi (1995) Relief of YY1 transcriptional repression by adenovirus E1A is mediated by E1A-associated protein p300. Genes Dev, 9, 1188-98.
Lee, K. Y., T. R. Broker & L. T. Chow (1998) Transcription factor YY1 represses cell-free replication from human Papillomavirus origins. J Virol, 72, 4911-7.
Lee, T., Y. Zhang & R. Schwartz (1994) Bifunctional transcriptional properties of YY1 in regulating muscle actin and c-myc gene expression during myogenesis. Oncogene, 9, 1047-1052.
Lee, T. C., Y. Shi & R. J. Schwartz (1992) Displacement of BrdUrd-induced YY1 by serum response factor activates skeletal alpha-actin transcription in embryonic myoblasts. Proc Natl Acad Sci U S A, 89, 9814-8.
Li, D. F., M. C. Zhang, H. J. Yang, Y. B. Zhu & X. Xu (2007) Beta-integrin mediates WSSV infection. Virology, 368, 122-32.
Li, F., M. Li, W. Ke, Y. Ji, X. Bian & X. Yan (2009) Identification of the immediate-early genes of white spot syndrome virus. Virology, 385, 267-74.
Li, W., L. M. Desmarets, G. M. De Gryse, S. Theuns, V. Van Tuan, K. Van Thuong, P. Bossier & H. J. Nauwynck (2015) Virus replication cycle of white spot syndrome virus in secondary cell cultures from the lymphoid organ of Litopenaeus vannamei. J Gen Virol, 96, 2844-54.
Lightner, D. V., R. M. Redman, C. R. Pantoja, K. F. J. Tang, B. L. Noble, P. Schofield, L. L. Mohney, L. M. Nunan & S. A. Navarro (2012) Historic emergence, impact and current status of shrimp pathogens in the Americas. Journal of Invertebrate Pathology, 110, 174-183.
Lin, F., H. Huang, L. Xu, F. Li & F. Yang (2011) Identification of three immediate-early genes of white spot syndrome virus. Archives of Virology, 156, 1611-4.
Liu, B. H., Y. C. Lin, C. S. Ho, C. C. Yang, Y. T. Chang, J. F. Chang, C. Y. Li, C. S. Cheng, J. Y. Huang, Y. F. Lee, M. H. Hsu, F. C. Lin, H. C. Wang, C. F. Lo, S. Y. Yang & H. C. Wang (2015a) A novel detection platform for shrimp white spot syndrome virus using an ICP11-dependent immunomagnetic reduction (IMR) assay. PLoS One, 10.
Liu, W. J., Y. S. Chang, W. T. Huang, I. T. Chen, K. C. Wang, G. H. Kou & C. F. Lo (2011) Penaeus monodon TATA box-binding protein interacts with the white spot syndrome virus transactivator IE1 and promotes its transcriptional activity. J Virol, 85, 6535-47.
Liu, W. J., Y. S. Chang, A. H. J. Wang, G. H. Kou & C. F. Lo (2007) White spot syndrome virus annexes a shrimp STAT to enhance expression of the immediate-early gene ie1. J Virol, 81, 1461-71.
Liu, W. J., Y. S. Chang, C. H. Wang, G. H. Kou & C. F. Lo (2005) Microarray and RT-PCR screening for white spot syndrome virus immediate-early genes in cycloheximide-treated shrimp. Virology, 334, 327-41.
Liu, W. J., Y. S. Chang, H. C. Wang, J. H. Leu, G. H. Kou & C. F. Lo (2008) Transactivation, dimerization, and DNA-binding activity of white spot syndrome virus immediate-early protein IE1. J Virol, 82, 11362-73.
Liu, W. J., C. F. Lo, G. H. Kou, J. H. Leu, Y. J. Lai, L. K. Chang & Y. S. Chang (2015b) The promoter of the white spot syndrome virus immediate-early gene WSSV108 is activated by the cellular KLF transcription factor. Dev Comp Immunol, 49, 7-18.
Lo, C.-F., Y.-S. Chang, S.-E. Peng & G.-H. Kou (2003) Major viral diseases of Penaeus monodon in Taiwan. JOURNAL-FISHERIES SOCIETY OF TAIWAN, 30, 1-14.
Lo, C., S. Peng, Y. Chang & G.-H. Kou. 2005. White spot syndrome—what we have learned about the virus and the disease. In Diseases in Asian Aquaculture V. Proceedings of the 5th Symposium on Diseases in Asian Aquaculture. Fish Health Section, Asian Fisheries Society, Manila, 421-433.
Lu, H., L. Ruan & X. Xu (2011) An immediate-early protein of white spot syndrome virus modulates the phosphorylation of focal adhesion kinase of shrimp. Virology, 419, 84-89.
Luo, J., X. Jiang, L. Cao, K. Dai, S. Zhang, X. Ge, X. Zhou & X. Lu (2014) Expression of YY1 correlates with progression and metastasis in esophageal squamous cell carcinomas. Onco Targets Ther, 7, 1753-9.
Margolis, D. M., M. Somasundaran & M. R. Green (1994) Human transcription factor YY1 represses human immunodeficiency virus type 1 transcription and virion production. J Virol, 68, 905-10.
Marks, H., J. J. van Duijse, D. Zuidema, M. C. van Hulten & J. M. Vlak (2005a) Fitness and virulence of an ancestral White Spot Syndrome Virus isolate from shrimp. Virus Res, 110, 9-20.
Marks, H., O. Vorst, A. M. van Houwelingen, M. C. van Hulten & J. M. Vlak (2005b) Gene-expression profiling of White spot syndrome virus in vivo. J Gen Virol, 86, 2081-100.
Martelli, F., C. Iacobini, M. Caruso & A. Felsani (1996) Characterization of two novel YY1 binding sites in the polyomavirus late promoter. J Virol, 70, 1433-8.
Momoyama, K., M. Hiraoka, H. Nakano & M. Sameshima (1998) Cryopreservation of penaeid rodshaped DNA virus (PRDV) and its survival in sea water at different temperatures. Fish pathology, 33, 95-96.
Muromoto, R., O. Ikeda, K. Okabe, S. Togi, S. Kamitani, M. Fujimuro, S. Harada, K. Oritani & T. Matsuda (2009) Epstein–Barr virus-derived EBNA2 regulates STAT3 activation. Biochemical and Biophysical Research Communications, 378, 439-443.
Muromoto, R., K. Okabe, M. Fujimuro, K. Sugiyama, H. Yokosawa, T. Seya & T. Matsuda (2006) Physical and functional interactions between STAT3 and Kaposi''s sarcoma-associated herpesvirus-encoded LANA. FEBS Letters, 580, 93-98.
Nadala, E. C., Jr. & P. C. Loh (1998) A comparative study of three different isolates of white spot virus. Dis Aquat Organ, 33, 231-4.
Natesan, S. & M. Z. Gilman (1993) DNA bending and orientation-dependent function of YY1 in the c-fos promoter. Genes Dev, 7, 2497-509.
Nguyen, A. T. V., C. K. Pham, H. T. T. Pham, H. L. Pham, A. H. Nguyen, L. T. Dang, H. A. Huynh, S. M. Cutting & T.-N. Phan (2014) Bacillus subtilis spores expressing the VP28 antigen: a potential oral treatment to protect Litopenaeus vannamei against white spot syndrome. FEMS Microbiology Letters, 358, 202-208.
Nilsen, P., M. Karlsen, K. Sritunyalucksana & S. Thitamadee (2017) White spot syndrome virus VP28 specific double-stranded RNA provides protection through a highly focused siRNA population. Sci Rep, 7.
Ning, D., X. Leng, Q. Li & W. Xu (2011) Surface-displayed VP28 on Bacillus subtilis spores induce protection against white spot syndrome virus in crayfish by oral administration. J Appl Microbiol, 111, 1327-36.
Nupan, B., A. Phongdara, M. Saengsakda, J. H. Leu & C. F. Lo (2011) Shrimp Pm-fortilin inhibits the expression of early and late genes of white spot syndrome virus (WSSV) in an insect cell model. Developmental & Comparative Immunology, 35, 469-475.
Oei, S. L. & Y. Shi (2001) Transcription factor Yin Yang 1 stimulates Poly(ADP-Ribosyl)ation and DNA repair. Biochemical and Biophysical Research Communications, 284, 450-454.
Otta, S., M. Poornima, P. E. Praveena, T. Bhuvaneswari, R. A. Raja, T. S. Kumar & S. Alavandi (2016) Prevention & Management of white spot disease of shrimp.
Pan, C., W. Wang, H. Yuan, L. Yang, B. Chen, D. Li & J. Chen (2017) The immediate early protein WSV187 can influence viral replication via regulation of JAK/STAT pathway in Drosophila. Developmental & Comparative Immunology, 72, 89-96.
Pradeep, B., P. Rai, S. A. Mohan, M. S. Shekhar & I. Karunasagar (2012) Biology, Host Range, Pathogenesis and Diagnosis of White spot syndrome virus. Indian J Virol, 23, 161-74.
Qi, W. (2002) Social and economic impacts of aquatic animal health problems in aquaculture in China. population, 250, 750.
Rameshthangam, P. & P. Ramasamy (2007) Antiviral activity of bis(2-methylheptyl)phthalate isolated from Pongamia pinnata leaves against white spot syndrome virus of Penaeus monodon Fabricius. Virus Research, 126, 38-44.
Ran, X., X. Bian, Y. Ji, X. Yan, F. Yang & F. Li (2013) White spot syndrome virus IE1 and WSV056 modulate the G(1)/S transition by binding to the host retinoblastoma protein. J Virol, 87, 12576-82.
Raught, B., B. Khursheed, A. Kazansky & J. Rosen (1994) YY1 represses beta-casein gene expression by preventing the formation of a lactation-associated complex. Molecular and cellular biology, 14, 1752-1763.
Rezai-Zadeh, N., X. Zhang, F. Namour, G. Fejer, Y. D. Wen, Y. L. Yao, I. Gyory, K. Wright & E. Seto (2003) Targeted recruitment of a histone H4-specific methyltransferase by the transcription factor YY1. Genes Dev, 17, 1019-29.
Sánchez-Paz, A. (2010) White spot syndrome virus: an overview on an emergent concern. Vet Res, 41.
Sano, K.-I., K. Maeda, M. Oki & Y. Maéda (2002) Enhancement of protein expression in insect cells by a lobster tropomyosin cDNA leader sequence. FEBS Letters, 532, 143-146.
Satheesh Kumar, S., R. Ananda Bharathi, J. J. S. Rajan, S. V. Alavandi, M. Poornima, C. P. Balasubramanian & A. G. Ponniah (2013) Viability of white spot syndrome virus (WSSV) in sediment during sun-drying (drainable pond) and under non-drainable pond conditions indicated by infectivity to shrimp. Aquaculture, 402–403, 119-126.
Schneider, I. (1972) Cell lines derived from late embryonic stages of Drosophila melanogaster. J Embryol Exp Morphol, 27, 353-65.
Seetang-Nun, Y., W. Jaroenram, S. Sriurairatana, R. Suebsing & W. Kiatpathomchai (2013) Visual detection of white spot syndrome virus using DNA-functionalized gold nanoparticles as probes combined with loop-mediated isothermal amplification. Molecular and Cellular Probes, 27, 71-79.
Seto, E., B. Lewist & T. Shenk (1993) Interaction between transcription factors Spl and YY1. Nature, 365, 462-464.
Shi, Y., E. Seto, L.-S. Chang & T. Shenk (1991) Transcriptional repression by YY1, a human GLI-Krüippel-related protein, and relief of repression by adenovirus E1A protein. Cell, 67, 377-388.
Sriton, A., N. Kanthong, W. Gangnonngiw, S. Sriurairatana, S. Ubol & T. W. Flegel (2009) Persistent expression of shrimp-virus antigens in two insect cell lines challenged with two shrimp viruses. Fish Pathology, 44, 86-93.
Stentiford, G. D., J. R. Bonami & V. Alday-Sanz (2009) A critical review of susceptibility of crustaceans to Taura syndrome, Yellowhead disease and White Spot Disease and implications of inclusion of these diseases in European legislation. Aquaculture, 291, 1-17.
Sudheer, N. S., R. Philip & I. S. B. Singh (2011) In vivo screening of mangrove plants for anti WSSV activity in Penaeus monodon, and evaluation of Ceriops tagal as a potential source of antiviral molecules. Aquaculture, 311, 36-41.
Takahashi, Y., T. Itami, M. Kondo, M. Maeda, R. Fujii, S. Tomonaga, K. Supamattaya & S. Boonyaratpalin (1994) Electron Microscopic Evidence of Bacilliform Virus Infection in Kuruma Shrimp (Penaeus japonicus). Fish Pathology, 29, 121-125.
Thitamadee, S., J. Srisala, S. Taengchaiyaphum & K. Sritunyalucksana (2014) Double-dose beta-glucan treatment in WSSV-challenged shrimp reduces viral replication but causes mortality possibly due to excessive ROS production. Fish Shellfish Immunol, 40, 478-84.
Trabucco, S. E., R. M. Gerstein & H. Zhang (2016) YY1 regulates the germinal center reaction by inhibiting apoptosis. J Immunol, 197, 1699-707.
Usheva, A. & T. Shenk (1994) TATA-binding protein-independent initiation: YY1, TFIIB, and RNA polymerase II direct basal transcription on supercoiled template DNA. Cell, 76, 1115-1121.
Vaughn, J. L., R. H. Goodwin, G. J. Tompkins & P. McCawley (1977) The establishment of two cell lines from the insect spodoptera frugiperda (lepidoptera; noctuidae). In Vitro, 13, 213-217.
Verbruggen, B., L. K. Bickley, R. van Aerle, K. S. Bateman, G. D. Stentiford, E. M. Santos & C. R. Tyler (2016) Molecular mechanisms of white spot syndrome virus infection and perspectives on treatments. Viruses, 8.
Vlak, J. M., J.-R. Bonami, T. W. Flegel, G.-H. Kou, D. V. Lightner, C.-f. Lo, P. C. Loh & P. J. Walker. 2002. Nimaviridae, a new virus family infecting aquatic invertebrates. In XIIth international congress of Virology, Paris.
Vlak, J. M., J. R. Bonami, T. W. Flegel, G. H. Kou, D. V. Lightner, C. F. Lo, P. C. Loh & P. W. Walker (2005) Nimaviridae. VIIIth Report of the International Committee on Taxonomy of Viruses, 187-192.
Walker, P. J. & C. Mohan (2009) Viral disease emergence in shrimp aquaculture: origins, impact and the effectiveness of health management strategies. Reviews in Aquaculture, 1, 125-154.
Wang, C. H., C. F. Lo, J. H. Leu, C. M. Chou, P. Y. Yeh, H. Y. Chou, M. C. Tung, C. F. Chang, M. S. Su & G. H. Kou (1995) Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon. Diseases of Aquatic Organisms, 23, 239-242.
Wang, P. H., Z. H. Gu, D. H. Wan, B. D. Liu, X. D. Huang, S. P. Weng, X. Q. Yu & J. G. He (2013) The shrimp IKK–NF-κB signaling pathway regulates antimicrobial peptide expression and may be subverted by white spot syndrome virus to facilitate viral gene expression. Cell Mol Immunol, 10, 423-36.
Wang, Q., B. L. White, R. M. Redman & D. V. Lightner (1999a) Per os challenge of Litopenaeus vannamei postlarvae and Farfantepenaeus duorarum juveniles with six geographic isolates of white spot syndrome virus. Aquaculture, 170, 179-194.
Wang, Y. G., M. D. Hassan, M. Shariff, S. M. Zamri & X. Chen (1999b) Histopathology and cytopathology of white spot syndrome virus (WSSV) in cultured Penaeus monodon from peninsular Malaysia with emphasis on pathogenesis and the mechanism of white spot formation. Diseases of Aquatic Organisms, 39, 1-11.
Wang, Z., H. K. Chua, A. A. Gusti, F. He, B. Fenner, I. Manopo, H. Wang & J. Kwang (2005) RING-H2 protein WSSV249 from white spot syndrome virus sequesters a shrimp ubiquitin-conjugating enzyme, PvUbc, for viral pathogenesis. J Virol, 79, 8764-72.
Wangman, P., R. Siriwattanarat, S. Longyant, C. Pengsuk, P. Sithigorngul & P. Chaivisuthangkura (2017) High sensitivity immunochromatographic strip test (ICP11 strip test) for white spot syndrome virus detection using monoclonal antibodies specific to ICP11 non-structural protein. Aquaculture, 470, 25-31.
White, B. L., P. J. Schofield, B. T. Poulos & D. V. Lightner (2002) A laboratory challenge method for estimating Taura syndrome virus resistance in selected lines of Pacific white shrimp Litopenaeus vannamei. Journal of the World Aquaculture Society, 33, 341-348.
Wu, J., Q. Lin, T. K. Lim, T. Liu & C. L. Hew (2007) White spot syndrome virus proteins and differentially expressed host proteins identified in shrimp epithelium by shotgun proteomics and cleavable isotope-coded affinity tag. J Virol, 81, 11681-9.
Xue, S., J. Wei, J. Li, X. Geng & J. Sun (2015) Effects of total ammonia, temperature and salinity on the mortality and viral replication of WSSV‐infected Chinese shrimp (Fenneropenaeus chinensis). Aquaculture Research.
Yan, D. C., S. L. Dong, J. Huang, X. M. Yu, M. Y. Feng & X. Y. Liu (2004) White spot syndrome virus (WSSV) detected by PCR in rotifers and rotifer resting eggs from shrimp pond sediments. Dis Aquat Organ, 59, 69-73.
Yang, F., J. He, X. Lin, Q. Li, D. Pan, X. Zhang & X. Xu (2001) Complete genome sequence of the shrimp white spot bacilliform virus. J Virol, 75, 11811-20.
Yang, W. M., C. Inouye, Y. Zeng, D. Bearss & E. Seto (1996) Transcriptional repression by YY1 is mediated by interaction with a mammalian homolog of the yeast global regulator RPD3. Proc Natl Acad Sci U S A, 93, 12845-50.
Yant, S. R., W. Zhu, D. Millinoff, J. L. Slightom, M. Goodman & D. L. Gumucio (1995) High affinity YY1 binding motifs: identification of two core types (ACAT and CCAT) and distribution of potential binding sites within the human beta globin cluster. Nucleic Acids Res, 23, 4353-62.
Yao, D., L. Ruan, H. Lu, H. Shi & X. Xu (2016) Shrimp STAT was hijacked by white spot syndrome virus immediate-early protein IE1 involved in modulation of viral genes. Fish Shellfish Immunol, 59, 268-275.
Zamanian-Daryoush, M., T. H. Mogensen, J. A. DiDonato & B. R. G. Williams (2000) NF-κB activation by double-stranded-RNA-activated Protein Kinase (PKR) is mediated through NF-κB-inducing kinase and IκB kinase. Mol Cell Biol, 20, 1278-90.
Zhang, J. Y., Q. H. Liu & J. Huang (2014) Multiple proteins of White spot syndrome virus involved in recognition of beta-integrin. J Biosci, 39, 381-8.
Zhang, X., C. Huang, X. Xu & C. L. Hew (2002) Identification and localization of a prawn white spot syndrome virus gene that encodes an envelope protein. Journal of General Virology, 83, 1069-1074.
Zhou, Q., R. W. Gedrich & D. A. Engel (1995) Transcriptional repression of the c-fos gene by YY1 is mediated by a direct interaction with ATF/CREB. J Virol, 69, 4323-30.
Zhu, F. & X. Zhang (2012) Protection of shrimp against White spot syndrome virus (WSSV) with β-1,3-d-glucan-encapsulated vp28-siRNA Particles. Marine Biotechnology, 14, 63-68.
王開立 (2016) 白蝦YY1及Dorsal蛋白質協同調控白點症病毒極早期基因的表現。國立臺灣大學生命科學院生化科技學系碩士論文。
吳柏軒 (2016a) 學者找到基因 抗病冷凍草蝦將問世。自由時報。
吳柏軒 (2016b) 篩選出抗病草蝦 成大學者要引領搶攻國際市場。自由時報。
吳智宇 (2016) 白點症病毒WSSV108蛋白質對極早期基因wssv304之轉錄調控。國立臺灣大學生命科學院生化科技學系碩士論文。
黃庭儀 (2014) 白蝦YY1調控白點症病毒極早期基因wssv126 (ie1)轉錄活性。國立臺灣大學生命科學院生化科技學系碩士論文。
楊舒涵 (2011) 白蝦Kruppel-like factor於白點症病毒感染時之功能性探討。國立臺灣大學生命科學院生化科技學系碩士論文。
蔡沛賜 (2014) 白蝦YY1調控白點症病毒極早期基因wssv108的機轉。國立臺灣大學生命科學院生化科技學系碩士論文。