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研究生:鍾易蓁
研究生(外文):Yi-Chen Chung
論文名稱:鮑魚菇蛋白 Pcy 對小鼠腹腔巨噬細胞的活化作用
論文名稱(外文):A Protein Pcy Isolated from Pleurotus cystidiosus Induces the Activation of Mouse Peritoneal Macrophages
指導教授:許輔許輔引用關係
指導教授(外文):Fuu Sheu
口試日期:2017-07-10
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:園藝暨景觀學系
學門:農業科學學門
學類:園藝學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:中文
論文頁數:82
中文關鍵詞:鮑魚菇免疫調節蛋白小鼠腹腔巨噬細胞類鐸受體
外文關鍵詞:Pleurotus cystidiosusimmunomodulatory proteinmouse peritoneal macrophageToll-like receptor
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鮑魚菇 (Pleurotus cystidiosus) 是臺灣常見菇類,為側耳科 (Pleurotaceae) 側耳屬 (Pleurotus) 真菌,過去文獻指出,鮑魚菇萃取物具有抗高血壓、抗癌細胞增生與降低血糖等多項生物活性,然而卻少有實驗使用純物質進行研究。因此本研究目的以純化鮑魚菇 14 kDa 之蛋白 (Pcy) 為材料,並研究 Pcy 對小鼠腹腔巨噬細胞 (peritoneal macrophage, pMø) 的免疫調節活性與活化路徑。
經實驗結果發現,將 12.5 μg/mL Pcy 與 pMø 共培養,可以活化 pMø 並增強 pMø 吞噬大腸桿菌之活性,增加表面抗原 CD86 與 MHC class II 表現,提高細胞激素 TNF-α 與 IL-1β 分泌量,同時也提高 TNF-α、IL-1β、IL-12p35、IL-12p40、CD38、EGR2 和 IL-10 等細胞激素之基因表現量。另外,Pcy 可以增加 M1 型 CCL3、CCL4 等趨化素基因之表現,以及 M2 型趨化素 CCL17 和 CCL22 基因表現量也會增加。在訊息傳導途徑方面,分別使用 TLR2 與 TLR4 基因剔除小鼠,發現 Pcy 會活化 TLR2-/- 小鼠腹腔巨噬細胞活化產生 TNF-α 與 IL-1β,然而於 TLR4-/- 小鼠之 TNF-α 與 IL-1β 分泌量皆不明顯,因此推測 Pcy 活化小鼠腹腔巨噬細胞之途徑和 TLR4 相關,然而還需進一步研究驗證。由以上實驗結果可知 Pcy 能活化小鼠腹腔巨噬細胞及其表面標誌,提升宿主免疫反應。
Abalone mushroom (Pleurotus cystidious) is one of the most popular edible mushrooms in Asia. Previous studies have shown that extracts from P. cystidious possessed several biological activities including antihypertension, antiproliferation of tumor cells and hypoglycemic effect. However, little research put emphasis on purified protein from P. cystidious. In this study, a 14 kDa immunomodulatory protein, Pcy, was purified from P. cystidious, and the effects of Pcy on activation, differentiation and TLR-signaling pathways of mouse peritoneal macrophages were investigated. According to the results, Pcy could induce phagocytic activity, increase the secretion of TNF-α and IL-1β, and enhance the expression of surface markers CD86 and MHC class II of mouse peritoneal macrophages. Pcy could upregulate mRNA expression of TNF-α, IL-1β, IL-12p35, IL-12p40, CD38, EGR2, and IL-10 cytokines. Pcy could also increase mRNA expression of both M1 and M2 type chemokine genes. Finally, TLR2 and TLR4 knockout mice were used to investigate the signaling pathway. Pcy could still induce macrophages to secrete TNF-α and IL-1β from TLR2 knockout mice, but the same activation was not obvious in TLR4 knockout mice. We suggest that the activation of Pcy on macrophage might relate to TLR4 signaling pathway, and more researches should be done for further confirmation. In conclusion, Pcy is an immunomodulatory protein, which could activated macrophages and strengthen the host immunity.
口試委員會審定書 Ⅰ
致謝 Ⅱ
摘要 Ⅳ
Abstract Ⅴ
目錄 Ⅵ
圖目錄 Ⅸ
第一章 前言 1
第一節 食藥用菇類之生理活性 1
1.1. 食藥用菇類的重要性 1
1.2. 食藥用菇類的抗腫瘤與免疫調節活性 1
1.3. 菇類中已知的生理活性成分 2
1.4. 具生理機能性的菇類蛋白 3
第二節 鮑魚菇簡介 4
2.1. 鮑魚菇分類與特性 4
2.2. 鮑魚菇之營養成分 5
2.3. 鮑魚菇已知成分與功能 5
第三節 免疫調節反應 8
3.1. 先天性免疫反應 9
3.1.1. 單核細胞 (Monocyte) 的生理功能 9
3.1.2. Toll-like receptors 與訊息傳導 10
3.1.3. TLR2 10
3.1.4. TLR4 11
3.1.5. TLR5 12
3.2. 巨噬細胞之免疫反應 12
3.3. 適應性免疫反應 13
3.4. 調節適應性免疫反應 13
3.4.1. 細胞激素 (cytokines) 14
3.4.2. 趨化素 (chemokines) 15
第四節 研究動機與目的 16
第二章 材料與方法 17
第一節 鮑魚菇免疫調節蛋白之純化與鑑定 17
1.1. 鮑魚菇之純化 17
1.2. SDS-PAGE 膠體電泳分析 19
1.3. 醣蛋白染色分析 20
1.4. 血球凝集活性 21
第二節 Pcy 活化小鼠腹腔巨噬細胞之免疫活性 21
第三節 小鼠腹腔巨噬細胞吞噬活性測試 25
第四節 小鼠腹腔巨噬細胞之抗原呈獻能力測試 27
第五節 Pcy 刺激之腹腔巨噬細胞基因表現量之測定 29
第六節 TLRs 與 Pcy 活化巨噬細胞之相關性 32
第七節 統計分析 34
第三章 結果 35
第一節 鮑魚菇蛋白Pcy之純化與性質 35
第二節 Pcy 對小鼠脾細胞之影響 36
第三節 Pcy 對小鼠腹腔巨噬細胞吞噬能力之影響 36
第四節 Pcy 對小鼠腹腔巨噬細胞抗原呈獻能力之影響 37
第五節 Pcy 增加小鼠腹腔巨噬細胞細胞激素之分泌 38
第六節 Pcy 對小鼠腹腔巨噬細胞之細胞激素基因表現量之影響 39
第七節 Pcy對小鼠腹腔巨噬細胞趨化素基因表現量之影響 40
第八節 Pcy活化小鼠巨噬細胞之相關路徑 41
第四章 討論 42
第一節 鮑魚菇蛋白 Pcy 之選擇與純化 42
第二節 Pcy 對小鼠脾臟細胞和巨噬細胞之影響 42
第三節 Pcy 可增加巨噬細胞吞噬能力及抗原呈獻能力 43
第四節 Pcy 可促進細胞激素基因之表現與分泌 44
第五節 Pcy 可增加巨噬細胞趨化素基因之表現 45
第六節 TLRS 路徑與 Pcy 活化巨噬細胞之相關性 46
第五章 結論 47
第六章 參考文獻 48
附錄 60
Aderem, A., & Underhill, D. M. (1999). Mechanisms of phagocytosis in macrophages. Annual review of immunology, 17(1), 593-623.
Akira, S., Takeda, K., & Kaisho, T. (2001). Toll-like receptors: critical proteins linking innate and acquired immunity. Nature immunology, 2(8), 675-680.
Akira, S., Uematsu, S., & Takeuchi, O. (2006). Pathogen recognition and innate immunity. Cell, 124(4), 783-801.
Auffray, C., Sieweke, M. H., & Geissmann, F. (2009). Blood monocytes: development, heterogeneity, and relationship with dendritic cells. Annual review of immunology, 27, 669-692.
Banchereau, J., Briere, F., Caux, C., Davoust, J., Lebecque, S., Liu, Y.-J., Pulendran, B., & Palucka, K. (2000). Immunobiology of dendritic cells. Annual review of immunology, 18(1), 767-811.
Belardelli, F. (1995). Role of interferons and other cytokines in the regulation of the immune response. Apmis, 103(1‐6), 161-179.
Biswas, S. K., & Mantovani, A. (2010). Macrophage plasticity and interaction with lymphocyte subsets: cancer as a paradigm. Nature immunology, 11(10), 889-896.
Blander, J. M., & Medzhitov, R. (2004). Regulation of phagosome maturation by signals from toll-like receptors. Science, 304(5673), 1014-1018.
Bowie, A. G., & Haga, I. R. (2005). The role of Toll-like receptors in the host response to viruses. Molecular immunology, 42(8), 859-867.
C Ooi, V. E., & Liu, F. (2000). Immunomodulation and anti-cancer activity of polysaccharide-protein complexes. Current medicinal chemistry, 7(7), 715-729.
Cao, Q.-Z., & Lin, Z.-b. (2004). Antitumor and anti-angiogenic activity of Ganoderma lucidum polysaccharides peptide. Acta Pharmacologica Sinica, 25, 833-838.
CFR Ferreira, I., A Vaz, J., Vasconcelos, M. H., & Martins, A. (2010). Compounds from wild mushrooms with antitumor potential. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents), 10(5), 424-436.
Chinetti-Gbaguidi, G., Colin, S., & Staels, B. (2015). Macrophage subsets in atherosclerosis. Nature Reviews Cardiology, 12(1), 10-17.
Ching, L., Abdullah, N., & Shuib, A. S. (2011). Characterization of antihypertensive peptides from pleurotus cystidiosus ok miller (abalone mushroom). In Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products (ICMBMP7), (pp. 314-323).
Clatza, A., Bonifaz, L. C., Vignali, D. A., & Moreno, J. (2003). CD40-induced aggregation of MHC class II and CD80 on the cell surface leads to an early enhancement in antigen presentation. The Journal of Immunology, 171(12), 6478-6487.
Conti, P., Kempuraj, D., Frydas, S., Kandere, K., Boucher, W., Letourneau, R., Madhappan, B., Sagimoto, K., Christodoulou, S., & Theoharides, T. (2003). IL-10 subfamily members: IL-19, IL-20, IL-22, IL-24 and IL-26. Immunology letters, 88(3), 171-174.
De Silva, D. D., Rapior, S., Fons, F., Bahkali, A. H., & Hyde, K. D. (2012). Medicinal mushrooms in supportive cancer therapies: an approach to anti-cancer effects and putative mechanisms of action. Fungal Diversity, 55(1), 1-35.
Dong, Y., Kwan, C.-Y., Chen, Z.-N., & Yang, M. M.-P. (1996). Antitumor effects of a refined polysaccharide peptide fraction isolated from Coriolus versicolor: in vitro and in vivo studies. Research communications in molecular pathology and pharmacology, 92(2), 140-148.
Endo, M., Beppu, H., Akiyama, H., Wakamatsu, K., Ito, S., Kawamoto, Y., Shimpo, K., Sumiya, T., Koike, T., & Matsui, T. (2010). Agaritine purified from Agaricus blazei Murrill exerts anti-tumor activity against leukemic cells. Biochimica et Biophysica Acta (BBA)-General Subjects, 1800(7), 669-673.
Ferrão, J., Bell, V., Calabrese, V., Pimentel, L., Pintado, M., & Fernandes, T. (2017). Impact of Mushroom Nutrition on Microbiota and Potential for Preventative Health. Journal of Food and Nutrition Research, 5(4), 226-233.
Fournier, B., & Philpott, D. J. (2005). Recognition of Staphylococcus aureus by the innate immune system. Clinical microbiology reviews, 18(3), 521-540.
Gao, Y., Gao, H., Chan, E., Tang, W., Xu, A., Yang, H., Huang, M., Lan, J., Li, X., & Duan, W. (2005). Antitumor activity and underlying mechanisms of ganopoly, the refined polysaccharides extracted from Ganoderma lucidum, in mice. Immunological investigations, 34(2), 171-198.
Gilgun-Sherki, Y., Rosenbaum, Z., Melamed, E., & Offen, D. (2002). Antioxidant therapy in acute central nervous system injury: current state. Pharmacological reviews, 54(2), 271-284.
Gordon, S. (2002). Pattern recognition receptors: doubling up for the innate immune response. Cell, 111(7), 927-930.
Gordon, S. (2003). Alternative activation of macrophages. Nature Reviews Immunology, 3(1), 23-35.
Gordon, S. (2007). The macrophage: past, present and future. European journal of immunology, 37(S1), S9-S17.
Han, S., Yoon, Y., Ahn, H., Lee, H., Lee, C., Yoon, W., Park, S., & Kim, H. (2003). Toll-like receptor-mediated activation of B cells and macrophages by polysaccharide isolated from cell culture of Acanthopanax senticosus. International immunopharmacology, 3(9), 1301-1312.
Hao-Chi, H., Chyong-Ing, H., Rong-Hwa, L., Chian-Liang, K., & Jung-Yaw, L. (1997). Fip-vvo, a new fungal immunomodulatory protein isolated from Volvariella volvacea. Biochemical Journal, 323(2), 557-565.
Hayashi, F., Smith, K. D., Ozinsky, A., Hawn, T. R., Eugene, C. Y., Goodlett, D. R., Eng, J. K., Akira, S., Underhill, D. M., & Aderem, A. (2001). The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature, 410(6832), 1099-1103.
Iwasaki, A., & Medzhitov, R. (2010). Regulation of adaptive immunity by the innate immune system. Science, 327(5963), 291-295.
Janeway Jr, C. A., & Medzhitov, R. (2002). Innate immune recognition. Annual review of immunology, 20(1), 197-216.
Jayasuriya, W., Suresh, T., Abeytunga, D. T. U., Fernando, G., & Wanigatunga, C. (2012). Oral hypoglycemic activity of culinary-medicinal mushrooms Pleurotus ostreatus and P. cystidiosus (higher basidiomycetes) in normal and alloxan-induced diabetic Wistar rats. International Journal of medicinal mushrooms, 14(4).
Jiménez-Dalmaroni, M. J., Radcliffe, C. M., Harvey, D. J., Wormald, M. R., Verdino, P., Ainge, G. D., Larsen, D. S., Painter, G. F., Ulevitch, R., & Beutler, B. (2015). Soluble human TLR2 ectodomain binds diacylglycerol from microbial lipopeptides and glycolipids. Innate immunity, 21(2), 175-193.
Johnson, J. G., & Jenkins, M. K. (1994). Minireview The role of anergy in peripheral T cell unresponsiveness. Life Sciences, 55(23), 1767-1780.
Kawai, T., & Akira, S. (2007). Signaling to NF-κB by Toll-like receptors. Trends in molecular medicine, 13(11), 460-469.
Kawai, T., & Akira, S. (2009). The roles of TLRs, RLRs and NLRs in pathogen recognition ARTICLE. International immunology, 21(4), 317-337.
Keswani, C., Mishra, S., Sarma, B. K., Singh, S. P., & Singh, H. B. (2014). Unraveling the efficient applications of secondary metabolites of various Trichoderma spp. Applied microbiology and biotechnology, 98(2), 533-544.
Kino, K., Yamashita, A., Yamaoka, K., Watanabe, J., Tanaka, S., Ko, K., Shimizu, K., & Tsunoo, H. (1989). Isolation and characterization of a new immunomodulatory protein, ling zhi-8 (LZ-8), from Ganoderma lucidium. Journal of Biological Chemistry, 264(1), 472-478.
Ko, J. L., Hsu, C. I., Lin, R. H., Kao, C. L., & Lin, J. Y. (1995). A New Fungal Immunomodulatory Protein, FIP‐fve Isolated from the Edible Mushroom, Flammulina velutipes and its Complete Amino Acid Sequence. The FEBS Journal, 228(2), 244-249.
Lam, S., & Ng, T. (2001a). First simultaneous isolation of a ribosome inactivating protein and an antifungal protein from a mushroom (Lyophyllum shimeji) together with evidence for synergism of their antifungal effects. Archives of Biochemistry and Biophysics, 393(2), 271-280.
Lam, S., & Ng, T. (2001b). Hypsin, a novel thermostable ribosome-inactivating protein with antifungal and antiproliferative activities from fruiting bodies of the edible mushroom Hypsizigus marmoreus. Biochemical and biophysical research communications, 285(4), 1071-1075.
Lau, C.-C., Abdullah, N., Shuib, A. S., & Aminudin, N. (2012). Proteomic analysis of antihypertensive proteins in edible mushrooms. Journal of agricultural and food chemistry, 60(50), 12341-12348.
Lee, S.-S., Wei, Y.-H., Chen, C.-F., Wang, S.-Y., & Chen, K.-Y. (1995). Antitumor effects of Ganoderma lucidum. 中醫藥雜誌, 6(1), 1-12.
Lemaitre В, N. Е., Michaut, L., & Reichhart, J. (1996). Ноffman JА The dorsoventral regulatory gene cassette spätzle/Toll/cactus controls the potent antifungal respon-se in Drosophila adults. Cell, 86, 973.
Lenschow, D. J., Walunas, T. L., & Bluestone, J. A. (1996). CD28/B7 system of T cell costimulation. Annual review of immunology, 14(1), 233-258.
Li, L., Ng, T., Song, M., Yuan, F., Liu, Z., Wang, C., Jiang, Y., Fu, M., & Liu, F. (2007). A polysaccharide–peptide complex from abalone mushroom (Pleurotus abalonus) fruiting bodies increases activities and gene expression of antioxidant enzymes and reduces lipid peroxidation in senescence-accelerated mice. Applied microbiology and biotechnology, 75(4), 863-869.
Li, N., Li, L., Fang, J. C., Wong, J. H., Ng, T. B., Jiang, Y., Wang, C. R., Zhang, N. Y., Wen, T. Y., & Qu, L. Y. (2012). Isolation and identification of a novel polysaccharide–peptide complex with antioxidant, anti-proliferative and hypoglycaemic activities from the abalone mushroom. Bioscience reports, 32(3), 221-228.
Li, Q., Wang, X., Chen, Y., Lin, J., & Zhou, X. (2010). Cytokines expression induced by Ganoderma sinensis fungal immunomodulatory proteins (FIP-gsi) in mouse spleen cells. Applied biochemistry and biotechnology, 162(5), 1403-1413.
Li, Y., Zhang, G., Ng, T. B., & Wang, H. (2010). A novel lectin with antiproliferative and HIV-1 reverse transcriptase inhibitory activities from dried fruiting bodies of the monkey head mushroom Hericium erinaceum. BioMed Research International, 2010.
Lis, H., & Sharon, N. (1986). Lectins as molecules and as tools. Annual review of biochemistry, 55(1), 35-67.
Liu, Ooi, V., & Chang, S. (1997). Free radical scavenging activities of mushroom polysaccharide extracts. Life Sciences, 60(10), 763-771.
Liu, Y.-C., Zou, X.-B., Chai, Y.-F., & Yao, Y.-M. (2014). Macrophage polarization in inflammatory diseases. International journal of biological sciences, 10(5), 520.
Mantovani, A., Locati, M., Vecchi, A., Sozzani, S., & Allavena, P. (2001). Decoy receptors: a strategy to regulate inflammatory cytokines and chemokines. Trends in immunology, 22(6), 328-336.
Mantovani, A., Sica, A., Sozzani, S., Allavena, P., Vecchi, A., & Locati, M. (2004). The chemokine system in diverse forms of macrophage activation and polarization. Trends in immunology, 25(12), 677-686.
Mantovani, A., Sozzani, S., Locati, M., Allavena, P., & Sica, A. (2002). Macrophage polarization: tumor-associated macrophages as a paradigm for polarized M2 mononuclear phagocytes. Trends in immunology, 23(11), 549-555.
Mau, J.-L., Beelman, R. B., & Ziegler, G. R. (1992). Effect of 10-oxo-trans-8-decenoic acid on growth of Agaricus bisporus. Phytochemistry, 31(12), 4059-4064.
Medzhitov, R. (2001). Toll-like receptors and innate immunity. Nature Reviews Immunology, 1(2), 135-145.
Menikpurage, I. P., Abeytunga, D., Jacobsen, N. E., & Wijesundara, R. (2009). An Oxidized Ergosterol from Pleurotus cystidiosus Active Against Anthracnose Causing Colletotrichumgloeosporioides. Mycopathologia, 167(3), 155.
Miyake, K. (2004). Innate recognition of lipopolysaccharide by Toll-like receptor 4–MD-2. Trends in microbiology, 12(4), 186-192.
Miyake, K. (2007). Innate immune sensing of pathogens and danger signals by cell surface Toll-like receptors. In Seminars in immunology, vol. 19 (pp. 3-10): Elsevier.
Mizuno, T., Sakai, T., & Chihara, G. (1995). Health foods and medicinal usages of mushrooms. Food Reviews International, 11(1), 69-81.
Mosser, D. M. (2003). The many faces of macrophage activation. Journal of leukocyte biology, 73(2), 209-212.
Mosser, D. M., & Edwards, J. P. (2008). Exploring the full spectrum of macrophage activation. Nature Reviews Immunology, 8(12), 958-969.
Mukherjee, S., Karmakar, S., & Babu, S. P. S. (2016). TLR2 and TLR4 mediated host immune responses in major infectious diseases: a review. Brazilian Journal of Infectious Diseases, 20(2), 193-204.
Murray, P. J., & Wynn, T. A. (2011). Protective and pathogenic functions of macrophage subsets. Nature Reviews Immunology, 11(11), 723-737.
Muzio, M., Polentarutti, N., Bosisio, D., Prahladan, M., & Mantovani, A. (2000). Toll-like receptors: a growing family of immune receptors that are differentially expressed and regulated by different leukocytes. Journal of leukocyte biology, 67(4), 450-456.
Nagai, Y., Akashi, S., Nagafuku, M., Ogata, M., Iwakura, Y., Akira, S., Kitamura, T., Kosugi, A., Kimoto, M., & Miyake, K. (2002). Essential role of MD-2 in LPS responsiveness and TLR4 distribution. Nature immunology, 3(7), 667-672.
Nathan, C., & Shiloh, M. U. (2000). Reactive oxygen and nitrogen intermediates in the relationship between mammalian hosts and microbial pathogens. Proceedings of the National Academy of Sciences, 97(16), 8841-8848.
Ng, S., Benjamin, J., McCarthy, N., Hedin, C., Koutsoumpas, A., Plamondon, S., Price, C., Hart, A., Kamm, M., & Forbes, A. (2011). Relationship between human intestinal dendritic cells, gut microbiota, and disease activity in Crohn''s disease. Inflammatory bowel diseases, 17(10), 2027-2037.
Ng, T. B., Lam, Y. W., & Wang, H. (2003). Calcaelin, a new protein with translation-inhibiting, antiproliferative and antimitogenic activities from the mosaic puffball mushroom Calvatia caelata. Planta medica, 69(03), 212-217.
Ngai, P. H., & Ng, T. (2003). Lentin, a novel and potent antifungal protein from shitake mushroom with inhibitory effects on activity of human immunodeficiency virus-1 reverse transcriptase and proliferation of leukemia cells. Life Sciences, 73(26), 3363-3374.
Oliver, C., Starke-Reed, P., Stadtman, E., Liu, G., Carney, J., & Floyd, R. (1990). Oxidative damage to brain proteins, loss of glutamine synthetase activity, and production of free radicals during ischemia/reperfusion-induced injury to gerbil brain. Proceedings of the National Academy of Sciences, 87(13), 5144-5147.
Park, B. S., Song, D. H., Kim, H. M., Choi, B.-S., Lee, H., & Lee, J.-O. (2009). The structural basis of lipopolysaccharide recognition by the TLR4–MD-2 complex. Nature, 458(7242), 1191-1195.
Pavlov, V., & Tracey, K. (2004). Neural regulators of innate immune responses and inflammation. Cellular and Molecular Life Sciences, 61(18), 2322-2331.
Peng, Y., Zhang, L., Zeng, F., & Kennedy, J. F. (2005). Structure and antitumor activities of the water-soluble polysaccharides from Ganoderma tsugae mycelium. Carbohydrate Polymers, 59(3), 385-392.
Pohleven, J., Obermajer, N., Sabotič, J., Anžlovar, S., Sepčić, K., Kos, J., Kralj, B., Štrukelj, B., & Brzin, J. (2009). Purification, characterization and cloning of a ricin B-like lectin from mushroom Clitocybe nebularis with antiproliferative activity against human leukemic T cells. Biochimica et Biophysica Acta (BBA)-General Subjects, 1790(3), 173-181.
Radi, R., Beckman, J. S., Bush, K. M., & Freeman, B. A. (1991). Peroxynitrite-induced membrane lipid peroxidation: the cytotoxic potential of superoxide and nitric oxide. Archives of Biochemistry and Biophysics, 288(2), 481-487.
Ren, D., Jiao, Y., Yang, X., Yuan, L., Guo, J., & Zhao, Y. (2015). Antioxidant and antitumor effects of polysaccharides from the fungus Pleurotus abalonus. Chemico-biological interactions, 237, 166-174.
Rock, F. L., Hardiman, G., Timans, J. C., Kastelein, R. A., & Bazan, J. F. (1998). A family of human receptors structurally related to Drosophila Toll. Proceedings of the National Academy of Sciences, 95(2), 588-593.
Rollins, B. J. (1997). Chemokines. Blood, 90(3), 909-928.
Schnare, M., Barton, G. M., Holt, A. C., Takeda, K., Akira, S., & Medzhitov, R. (2001). Toll-like receptors control activation of adaptive immune responses. Nature immunology, 2(10), 947-950.
Serbina, N. V., Jia, T., Hohl, T. M., & Pamer, E. G. (2008). Monocyte-mediated defense against microbial pathogens. Annu. Rev. Immunol., 26, 421-452.
Seth, R. B., Sun, L., & Chen, Z. J. (2006). Antiviral innate immunity pathways. Cell research, 16(2), 141-147.
Sheu, F., Chien, P.-J., Hsieh, K.-Y., Chin, K.-L., Huang, W.-T., Tsao, C.-Y., Chen, Y.-F., Cheng, H.-C., & Chang, H.-H. (2009). Purification, cloning, and functional characterization of a novel immunomodulatory protein from Antrodia camphorata (bitter mushroom) that exhibits TLR2-dependent NF-κB activation and M1 polarization within murine macrophages. Journal of agricultural and food chemistry, 57(10), 4130-4141.
Shi, X., Zhao, Y., Jiao, Y., Shi, T., & Yang, X. (2013). ROS-dependent mitochondria molecular mechanisms underlying antitumor activity of Pleurotus abalonus acidic polysaccharides in human breast cancer MCF-7 cells. PLoS One, 8(5), e64266.
Sindrilaru, A., Peters, T., Wieschalka, S., Baican, C., Baican, A., Peter, H., Hainzl, A., Schatz, S., Qi, Y., & Schlecht, A. (2011). An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice. The Journal of clinical investigation, 121(3), 985-997.
Smetanina, O. F., Kalinovsky, A. I., Khudyakova, Y. V., Pivkin, M. V., Dmitrenok, P. S., Fedorov, S. N., Ji, H., Kwak, J.-Y., & Kuznetsova, T. A. (2007). Indole alkaloids produced by a marine fungus isolate of Penicillium janthinellum Biourge. Journal of natural products, 70(6), 906-909.
Swirski, F. K., Nahrendorf, M., Etzrodt, M., Wildgruber, M., Cortez-Retamozo, V., Panizzi, P., Figueiredo, J.-L., Kohler, R. H., Chudnovskiy, A., & Waterman, P. (2009). Identification of splenic reservoir monocytes and their deployment to inflammatory sites. Science, 325(5940), 612-616.
Tadashi, K., SHIOSE, Y., NAGAI, K., & SAKUSHIMA, M. (1992). Polysaccharides in Fungi. XXIX. Structural Features of Two Antitumor Palysaccharides from the Fruiting Bodies of Armillariella tabescens. Chemical and pharmaceutical bulletin, 40(8), 2212-2214.
Takeda, K., & Akira, S. (2004). TLR signaling pathways. In Seminars in immunology, vol. 16 (pp. 3-9): Elsevier.
Takeda, K., & Akira, S. (2005). Toll-like receptors in innate immunity. International immunology, 17(1), 1-14.
Tanaka, S., Ko, K., Kino, K., Tsuchiya, K., Yamashita, A., Murasugi, A., Sakuma, S., & Tsunoo, H. (1989). Complete amino acid sequence of an immunomodulatory protein, ling zhi-8 (LZ-8). An immunomodulator from a fungus, Ganoderma lucidium, having similarity to immunoglobulin variable regions. Journal of Biological Chemistry, 264(28), 16372-16377.
Tobias, P. S., Soldau, K., Gegner, J. A., Mintz, D., & Ulevitch, R. J. (1995). Lipopolysaccharide binding protein-mediated complexation of lipopolysaccharide with soluble CD14. Journal of Biological Chemistry, 270(18), 10482-10488.
Tripathi, P., & Aggarwal, A. (2006). NF-kB transcription factor: a key player in the generation of immune response. CURRENT SCIENCE-BANGALORE-, 90(4), 519.
Uematsu, S., Fujimoto, K., Jang, M. H., Yang, B.-G., Jung, Y.-J., Nishiyama, M., Sato, S., Tsujimura, T., Yamamoto, M., & Yokota, Y. (2008). Regulation of humoral and cellular gut immunity by lamina propria dendritic cells expressing Toll-like receptor 5. Nature immunology, 9(7), 769-776.
Uematsu, S., Jang, M. H., Chevrier, N., Guo, Z., Kumagai, Y., Yamamoto, M., Kato, H., Sougawa, N., Matsui, H., & Kuwata, H. (2006). Detection of pathogenic intestinal bacteria by Toll-like receptor 5 on intestinal CD11c+ lamina propria cells. Nature immunology, 7(8), 868-874.
Underhill, D. M., Ozinsky, A., Smith, K. D., & Aderem, A. (1999). Toll-like receptor-2 mediates mycobacteria-induced proinflammatory signaling in macrophages. Proceedings of the National Academy of Sciences, 96(25), 14459-14463.
Van Furth, R., & Cohn, Z. A. (1968). The origin and kinetics of mononuclear phagocytes. The Journal of experimental medicine, 128(3), 415.
Verreck, F. A., de Boer, T., Langenberg, D. M., Hoeve, M. A., Kramer, M., Vaisberg, E., Kastelein, R., Kolk, A., de Waal-Malefyt, R., & Ottenhoff, T. H. (2004). Human IL-23-producing type 1 macrophages promote but IL-10-producing type 2 macrophages subvert immunity to (myco) bacteria. Proceedings of the National Academy of Sciences of the United States of America, 101(13), 4560-4565.
Visintin, A., Latz, E., Monks, B. G., Espevik, T., & Golenbock, D. T. (2003). Lysines 128 and 132 enable lipopolysaccharide binding to MD-2, leading to Toll-like receptor-4 aggregation and signal transduction. Journal of Biological Chemistry, 278(48), 48313-48320.
Visintin, A., Mazzoni, A., Spitzer, J. A., & Segal, D. M. (2001). Secreted MD-2 is a large polymeric protein that efficiently confers lipopolysaccharide sensitivity to Toll-like receptor 4. Proceedings of the National Academy of Sciences, 98(21), 12156-12161.
Wang, C. R., Ng, T. B., Li, L., Fang, J. C., Jiang, Y., Wen, T. Y., Qiao, W. T., Li, N., & Liu, F. (2011). Isolation of a polysaccharide with antiproliferative, hypoglycemic, antioxidant and HIV‐1 reverse transcriptase inhibitory activities from the fruiting bodies of the abalone mushroom Pleurotus abalonus. Journal of Pharmacy and Pharmacology, 63(6), 825-832.
Wang, H., & Ng, T. (2000). Flammulin: a novel ribosome-inactivating protein from fruiting bodies of the winter mushroom Flammulina velutipes. Biochemistry and Cell Biology, 78(6), 699-702.
Wang, H., & Ng, T. (2001). Isolation of pleuturegin, a novel ribosome-inactivating protein from fresh sclerotia of the edible mushroom Pleurotus tuber-regium. Biochemical and biophysical research communications, 288(3), 718-721.
Wang, H., Ng, T., & Liu, Q. (2002). Isolation of a new heterodimeric lectin with mitogenic activity from fruiting bodies of the mushroom Agrocybe cylindracea. Life Sciences, 70(8), 877-885.
Wang, H., & Ng, T. B. (2001). Isolation and characterization of velutin, a novel low-molecular-weight ribosome-inactivating protein from winter mushroom (Flammulina velutipes) fruiting bodies. Life Sciences, 68(18), 2151-2158.
Wasser, S. P. (2011). Current findings, future trends, and unsolved problems in studies of medicinal mushrooms. Applied microbiology and biotechnology, 89(5), 1323-1332.
Wasser, S. P. (2014). Medicinal mushroom science: Current perspectives, advances, evidences, and challenges. Biomed J, 37(6), 345-356.
Wasser, S. P., & Weis, A. L. (1999). Medicinal properties of substances occurring in higher basidiomycetes mushrooms: current perspectives (review). International Journal of medicinal mushrooms, 1(1).
Wong, J. H., Wang, H., & Ng, T. (2008). Marmorin, a new ribosome inactivating protein with antiproliferative and HIV-1 reverse transcriptase inhibitory activities from the mushroom Hypsizigus marmoreus. Applied microbiology and biotechnology, 81(4), 669-674.
Wynn, T. A., Chawla, A., & Pollard, J. W. (2013). Macrophage biology in development, homeostasis and disease. Nature, 496(7446), 445-455.
Yang, B.-K., Kim, G.-N., Jeong, Y.-T., Jeong, H., Mehta, P., & Song, C.-H. (2008). Hypoglycemic effects of exo-biopolymers produced by five different medicinal mushrooms in STZ-induced diabetic rats. Mycobiology, 36(1), 45-49.
Yang, J.-H., Lin, H.-C., & Mau, J.-L. (2001). Non-volatile taste components of several commercial mushrooms. Food chemistry, 72(4), 465-471.
Yang, J.-H., Lin, H.-C., & Mau, J.-L. (2002). Antioxidant properties of several commercial mushrooms. Food chemistry, 77(2), 229-235.
Yang, N., Li, D.-F., Feng, L., Xiang, Y., Liu, W., Sun, H., & Wang, D.-C. (2009). Structural basis for the tumor cell apoptosis-inducing activity of an antitumor lectin from the edible mushroom Agrocybe aegerita. Journal of molecular biology, 387(3), 694-705.
Yang, Y., & Wilson, J. M. (1996). CD40 ligand-dependent T cell activation: requirement of B7-CD28 signaling through CD40. Science, 273(5283), 1862.
Zheng, Y., Pang, H., Wang, J., Shi, G., & Huang, J. (2015). New apoptosis-inducing sesquiterpenoids from the mycelial culture of Chinese edible fungus Pleurotus cystidiosus. Journal of agricultural and food chemistry, 63(2), 545-551.
Zhou, D., Huang, C., Lin, Z., Zhan, S., Kong, L., Fang, C., & Li, J. (2014). Macrophage polarization and function with emphasis on the evolving roles of coordinated regulation of cellular signaling pathways. Cellular signalling, 26(2), 192-197.
王谦, & 贾震. (2010). 食药用真菌的药理作用研究进展. 医学研究与教育, 27(5), 67-70.
江金標. (1973). 鮑魚菇栽培之基礎研究. 中國園藝, 19(6), 410-415.
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