(3.237.178.91) 您好!臺灣時間:2021/03/02 21:41
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:李鍾山
研究生(外文):Chung- San Lee
論文名稱:設計具有二聚體結構之單鏈抗體對抗蝦白點病毒之研究
論文名稱(外文):Study of single chain antibody fragment designed with dimeric constructs against the white spot syndrome virus in shrimp
指導教授:韓玉山韓玉山引用關係
口試日期:2017-07-28
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:漁業科學研究所
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:英文
論文頁數:65
中文關鍵詞:白點病毒大腸桿菌表現系統中和抗體單鍊抗體片段亮胺酸拉鍊二聚化
外文關鍵詞:white spot syndrome virusE. coli expression systemneutralizing antibodysingle-chain variable fragmentleucine zipperdimerization
相關次數:
  • 被引用被引用:0
  • 點閱點閱:86
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
蝦類養殖產業是水產養殖中重要的一部分,每年有高達220億美金的產值。但養蝦產業卻常受到疾病侵擾,導致大量的損失。蝦的白點病(white spot disease, WSD)尤為嚴重,WSD典型病徵是在病蝦的頭胸部、附肢及體表出現白色斑點,且在病徵出現後短短10天內,死亡率高達90-100%。WSD是由白點病毒(white spot syndrome virus, WSSV)所導致。超過40個WSSV病毒蛋白已經被了解,包含結構和非結構蛋白。其中,VP28是WSSV主要的包膜蛋白,被認為在病毒感染細胞的過程中,扮演重要的角色。
WSD發現20多餘年,一直沒有有效的解決辦法。美國Transcending Biotechnologies Inc. 研發了單株抗體AP-1,能夠和WSSV的VP28緊密結合,阻擋病毒感染細胞的過程,進而防止蝦類受到WSSV的感染。但單株抗體利用動物細胞生產的成本太高,不適合大規模應用在養殖現場。先前冠群生技公司利用AP-1的變異片段(variable fragment)製成了單鏈抗體sAP-1,完整保留了AP-1的抗原結合位,成功在大腸桿菌原核表現系統中大量表現。但在先前的研究中發現了sAP-1的抗病毒效果不如AP-1。合理懷疑,具有兩個抗原結合位的AP-1,比只有一個抗原結合位的sAP-1,能跟病毒結合的更牢固,因此具有更好的抗病毒效果。
因此,實驗嘗試在sAP-1蛋白後端接上亮胺酸拉鏈( leucine zipper )胺基酸序列,使sAP-1形成二聚體dsAP-1,進而提升抗病毒效果。以利後續應用微生物製造的dsAP-1在大規模養殖中,防止WSSV的感染。
在ELISA的結果中發現,dsAP-1跟sAP-1相比,具有較好的WSSV結合能力。而在攻毒實驗的結果中,dsAP-1組比起sAP-1組,具有較高的存活率。顯示sAP-1形成二聚體dsAP-1後,抗白點病毒的能力有些提升的趨勢。
Shrimp farming is an important part of aquaculture industry, with output value reaching 22 billion U.S. dollar annually. However, pathogens always devastate shrimp farming, causing enormous economic losses. White spot disease (WSD) is one of the most potent and widespread disease, with the typical syndrome white spots on the cuticle of the shrimp cephalothorax, carapace and appendage. WSD can cause up to 90-100% mortality within 10 days after symptoms appear. WSD is caused by white spot syndrome virus (WSSV). More than 40 viral proteins of WSSV were identified and studied, including structural and non-structural proteins. One of them, VP28, is a major envelope protein of WSSV, may play an important role in the infection process of WSD.
WSD has been found for more than 20 years, but there are still no available treatment measures. Transcending Biotechnologies Inc. (U.S.A.) developed a monoclonal antibody, AP-1. AP-1 can bind to VP28 tightly and interfere the interaction between WSSV and host cell, thus preventing shrimp from infection of WSSV. However, the production cost of monoclonal antibody is too high to be practically utilized for shrimp farming. The variable fragment of AP-1, which contains complete antigen-binding site, was synthesized as the single chain peptide, sAP-1. sAP-1 was successfully expressed in huge quantity by E. coli expression system. However, previous studies found that the anti-WSSV activity of monomeric sAP-1 is weaker than that of the dimeric AP-1. We speculate that AP-1 with two antigen-binding site may bind to VP28 more tightly than sAP-1 with only one antigen-binding site, thus AP-1 has stronger anti-WSSV ability.
For those reasons, this study aims to modify sAP-1 by adding leucine zipper after its C-terminus, making two sAP-1 monomers forming dimmer, dsAP-1. The anti-WSSV efficacy of ds-AP-1 is proposed to be improved. The dsAP-1 may be used at large scale shrimp farming to prevent WSSV infection.
The result of ELISA showed that the WSSV binding ability of dsAP-1 was slightly better than that of sAP-1. WSSV viral challenge also showed that the survival rate of dsAP-1 group was better than sAP-1 group at the same amount of protein. The dimerized sAP-1 has better anti-WSSV activity than sAP-1.
致謝 i
中文摘要 ii
ABSTRACT iii
CONTENTS v
LIST OF FIGURES vii
LIST OF TABLES viii
Chapter 1 Introduction 1
1.1 Shrimp farming 1
1.2 White Spot Syndrome Virus 2
1.3 Neutralization antibody & Single chain antibody fragment 4
1.4 Single chain antibody fragment dimerization 7
Chapter 2 Materials and methods 9
2.1 Experimental animals and WSSV 9
2.2 Expression and purification of proteins 9
2.3 Biochemical analysis of recombinant proteins 14
2.4 Analysis of neutralizing activity 15
2.5 WSSV viral challenge assay 16
Chapter 3 Results 18
3.1 Proteins expression 18
3.2 Biochemical analysis of recombinant proteins 19
3.3 Analysis of neutralizing activity 19
3.4 WSSV viral challenge assay 20
Chapter 4 Discussion 22
4.1 Proteins expression 22
4.2 Biochemical analysis of recombinant proteins 23
4.3 Analysis of neutralizing activity 24
4.4 WSSV viral challenge assay 24
Chapter 5 Conclusion 26
References 27
Figures 43
Tables 61
Ahmad, Z. A., Yeap, S. K., Ali, A. M., Ho, W. Y., Alitheen, N. B., & Hamid, M. (2012). scFv antibody: principles and clinical application. Clin Dev Immunol, 2012, 980250.
Aly, S. M., Ahmed, Y. A. G., Ghareeb, A. A. A., & Mohamed, M. F. (2008). Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia nilotica (Oreochromis niloticus) to challenge infections. Fish Shellfish Immunol, 25(1), 128-136.
Bell, T. A., & Lightner, D. V. (1984). IHHN virus: infectivity and pathogenicity studies in Penaeus stylirostris and Penaeus vannamei. Aquaculture, 38(3), 185-194.
Biedendieck, R., Borgmeier, C., Bunk, B., Stammen, S., Scherling, C., Meinhardt, F., Wittmann, C., Jahn, D. (2011). Systems biology of recombinant protein production using Bacillus megaterium. Methods Enzymol, 500, 165-195.
Bonami, J. R., Hasson, K. W., Mari, J., Poulos, B. T., & Lightner, D. V. (1997). Taura syndrome of marine penaeid shrimp: characterization of the viral agent. J Gen Virol, 78 ( Pt 2), 313-319.
Boss, M. A., Kenten, J. H., Wood, C. R., & Emtage, J. S. (1984). Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised in E. coli. Nucleic Acids Res, 12(9), 3791-3806.
Chang, C. F., Su, M. S., Chen, H. Y., Liao, I. C. (2003) Dietary β-1,3-glucan effectively improves immunity and survival of Penaeus monodon challenged with white spot syndrome virus. Fish & Shellfish Immunology. 15: 297-310.
Chang, P. S., Lo, C. F., Wang, Y. C., & Kou, G. H. (1996). Identification of white spot syndrome associated baculovirus (WSBV) target organs in the shrimp Penaeus monodon by in situ hybridization. Dis Aquat Organ, 27(2), 131-139.
Chantanachookin, C., Boonyaratpalin, S., Kasornchandra, J., Direkbusarakom, S., Ekpanithanpong, U., Supamataya, K., Sriurairatana, S., Flegel, T. W. (1993). Histology and ultrastructure reveal a new granulosis-like virus in Penaeus monodon affected by yellow-head disease. Dis Aquat Organ, 17, 145-145.
Chen, L. Y. (2015). Single chain antibody fragment against the horizontal transmission of white spot syndrome virus in Penaeus vannamei (Master''s thesis). Institute of Fisheries Science, College of Life Science, National Taiwan University, 1-61.
Chesson, A., Franklin, A., Aumaître, A., Sköld, O., Leclercq, R., von Wright, A., Guillot, J. F. (2002). Opinion of the scientific committee on animal nutrition on the criteria for assessing the safety of microorganisms resistant to antibiotics of human and veterinary importance. Directorate C-Scientific Opinions.
Chiang, H. C., & Lo, C. F. (1995). Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan. Dis Aquat Organ, 23, 165-173.
Chou, H. Y., Huang, C. Y., Lo, C. F., & Kou, G. H. (1998). Studies on transmission of white spot syndrome associated baculovirus (WSBV) in Penaeus monodon and P. japonicus via waterborne contact and oral ingestion. Aquaculture, 164(1), 263-276.
Cutting, S. M. (2011). Bacillus probiotics. Food Microbiol, 28(2), 214-220.
de la Pena, L. D., Lavilla-Pitogo, C. R., Villar, C. B., Paner, M. G., Sombito, C. D., & Capulos, G. C. (2007). Prevalence of white spot syndrome virus (WSSV) in wild shrimp Penaeus monodon in the Philippines. Dis Aquat Organ, 77(3), 175-179.
Durand, S. V., Tang, K. F. J., & Lightner, D. V. (2000). Frozen commodity shrimp: potential avenue for introduction of white spot syndrome virus and yellow head virus. J Aquat Anim Health, 12(2), 128-135.
Elliston, J. F., Mcmicken, H. W., & Hansen, T. N. (1994). Expression and Purification of Glutathione-Reductase as a Fusion Protein by the Baculovirus Vector System. Pediatric Research, 35: A66-A66.
Escobedo-Bonilla, C. M., Alday-Sanz, V., Wille, M., Sorgeloos, P., Pensaert, M. B., & Nauwynck, H. J. (2008). A review on the morphology, molecular characterization, morphogenesis and pathogenesis of white spot syndrome virus. J Fish Dis, 31(1), 1-18.
Escobedo-Bonilla, C. M., Wille, M., Sanz, V. A., Sorgeloos, P., Pensaert, M. B., & Nauwynck, H. J. (2005). In vivo titration of white spot syndrome virus (WSSV) in specific pathogen-free Litopenaeus vannamei by intramuscular and oral routes. Dis Aquat Organ, 66(2), 163-170.
Flegel, T. W., Fegan, D. F., & Sriurairatana, S. (1995). Environmental control of infectious shrimp diseases in Thailand. Diseases in Asian aquaculture II, 65-79.
Flegel, T. W., Lightner, D. V., Lo, C. F., & Owens, L. (2008). Shrimp disease control: past, present and future. Diseases in Asian Aquaculture VI. Fish Health Section, Asian Fisheries Society. Manila, Philippines, 355-378.
Frangioni, J. V., & Neel, B. G. (1993). Solubilization and Purification of Enzymatically Active Glutathione-S-Transferase (Pgex) Fusion Proteins. Analytical Biochemistry, 210: 179-87.
Gao, W., Cai, L. T., Xu, X. D., Fan, J. X., Xue, X. L., Yan, X. J., Qu, Q. R.,Wang, X. H., Zhang, C., & Wu, G. Q. (2014). Anti-CTGF Single-Chain Variable Fragment Dimers Inhibit Human Airway Smooth Muscle (ASM) Cell Proliferation by Down-Regulating p-Akt and p-mTOR Levels. Plos One, 9(12).
Guo, J., Luo, Y., Fan, D., Yang, B., Gao, P., Ma, X., Zhu, C. (2010). Medium optimization based on the metabolic-flux spectrum of recombinant Escherichia coli for high expression of human-like collagen II. Biotechnol Appl Biochem, 57(2), 55-62.
Hebert, G. A., Pelham, P. L., & Pittman, B. (1973). Determination of the optimal ammonium sulfate concentration for the fractionation of rabbit, sheep, horse, and goat antisera. Applied microbiology, 25(1), 26-36.
Hsieh, C. Y., Chuang, P. C., Chen, L. C., Tu, C., Chien, M. S., Huang, K. C., Kao, H. F., Tung, M. C., Tsai, S. S. (2006). Infectious hypodermal and haematopoietic necrosis virus (IHHNV) infections in giant freshwater prawn, Macrobrachium rosenbergii. Aquaculture, 258(1), 73-79.
Hu, X., O''Hara, L., White, S., Magner, E., Kane, M., & Wall, J. G. (2007). Optimisation of production of a domoic acid-binding scFv antibody fragment in Escherichia coli using molecular chaperones and functional immobilisation on a mesoporous silicate support. Protein Expr Purif, 52(1), 194-201.
Huang, C. C., & Song, Y. L. (1999). Maternal transmission of immunity to white spot syndrome associated virus (WSSV) in shrimp (Penaeus monodon). Dev Comp Immunol, 23(7-8), 545-552.
Huang, J., Yu, J., Song, X. L., Kong, J., & Yang, C. H. (1995). Studies on fine structure, nucleic acid, polypeptide and serology of hypodermal and hematopoietic necrosis baculovirus of penaeid shrimp. Mar. Fish Res, 16, 11-23.
Huhn, C., Selman, M. H. J., Ruhaak, L. R., Deelder, A. M., & Wuhrer, M. (2009). IgG glycosylation analysis. Proteomics, 9(4), 882-913.
Huston, J. S., George, A. J., Adams, G. P., Stafford, W. F., Jamar, F., Tai, M. S., McCartney, J. E., Oppermann, H., Heelan, B. T., Peters, A. M. (1996). Single-chain Fv radioimmunotargeting. The quarterly journal of nuclear medicine: official publication of the Italian Association of Nuclear Medicine (AIMN) and the International Association of Radiopharmacology (IAR), 40(3), 320-333.
Ishibashi, N., & Yamazaki, S. (2001). Probiotics and safety. Am J Clin Nutr, 73(2), 465s-470s.
Jiravanichpaisal, P., Bangyeekhun, E., Söderhall, K., & Söderhall, I. (2001). Experimental infection of white spot syndrome virus in freshwater crayfish Pacifastacus leniusculus. Dis Aquat Organ, 47(2), 151-157.
Jordan, E., Al-Halabi, L., Schirrmann, T., Hust, M., & Dubel, S. (2007). Production of single chain Fab (scFab) fragments in Bacillus megaterium. Microb Cell Fact, 6, 38.
Jordan, E., Hust, M., Roth, A., Biedendieck, R., Schirrmann, T., Jahn, D., Dubel, S. (2007). Production of recombinant antibody fragments in Bacillus megaterium. Microb Cell Fact, 6, 2.
Kasornchandra, J., Boonyaratpalin, S., & Itami, T. (1998). Detection of white-spot syndrome in cultured penaeid shrimp in Asia: Microscopic observation and polymerase chain reaction. Aquaculture, 164(1), 243-251.
Kim, C. K., Kim, P. K., Sohn, S. G., Sim, D. S., Park, M. A., Heo, M. S., Lee, T. H., Lee, J. D., Jun, H. K., Jang, K. L. (1998). Development of a polymerase chain reaction (PCR) procedure for the detection of baculovirus associated with White Spot Syndrome (WSBV) in penaeid shrimp. J Fish Dis, 21(1), 11-17.
King, T. P. (1972). Separation of proteins by ammonium sulfate gradient solubilization. Biochemistry, 11(3), 367-371.
Klasse, P. J. (2014). Neutralization of virus infectivity by antibodies: old problems in new perspectives. Advances in Biology, 2014.
Knobloch, D., Ostermann, K., & Rodel, G. (2012). Production, secretion, and cell surface display of recombinant Sporosarcina ureae S-layer fusion proteins in Bacillus megaterium. Appl Environ Microbiol, 78(2), 560-567.
Korneli, C., Biedendieck, R., David, F., Jahn, D., & Wittmann, C. (2013). High yield production of extracellular recombinant levansucrase by Bacillus megaterium. Appl Microbiol Biotechnol, 97(8), 3343-3353.
Landschulz, W. H., Johnson, P. F., & Mcknight, S. L. (1988). The Leucine Zipper - a Hypothetical Structure Common to a New Class of DNA-Binding Proteins. Science, 240, 1759-64.
Lescar, J., Stouracova, R., Riottot, M. M., Chitarra, V., Brynda, J., Fabry, M., Horejsi, M., Sedlacek, J., Bentley, G. A. (1997). Three-dimensional structure of an Fab-peptide complex: structural basis of HIV-1 protease inhibition by a monoclonal antibody. J Mol Biol, 267(5), 1207-1222.
Liao, I. C., Peng, C.(2016). Biologics that inhibiting white spot syndrome virus and their application in shrimp farming. Progress of Shrimp and Prawn Aquaculture in the World. 337-353.
Lightner, D. V. (1996). A handbook of shrimp pathology and diagnostic procedures for diseases of cultured penaeid shrimp.
Lightner, D. V. (1999). The penaeid shrimp viruses TSV, IHHNV, WSSV, and YHV: current status in the Americas, available diagnostic methods, and management strategies. Journal of Applied Aquaculture, 9(2), 27-52.
Lo, C. F., & Kuo, G. H. (1998). Virus-associated white spot syndrome of shrimp in Taiwan. Fish Pathology (Japan).
Lo, C. F., Leu, J. H., Ho, C. H., Chen, C. N., Peng, S. E., Chen, Y. T., Chou, C. M., Yeh, P. Y., Huang, C. J., Chou, H. Y. (1996). Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimps using polymerase chain reaction. Dis Aquat Organ, 25, 133-141.
Macias-Rodriguez, N. A., Manon-Rios, N., Romero-Romero, J. L., Camacho-Beltran, E., Magallanes-Tapia, M. A., Leyva-Lopez, N. E., Magallón-Barajas, F. J., Perez-Enriquez, R., Sánchez-González, S. (2014). Prevalence of viral pathogens WSSV and IHHNV in wild organisms at the Pacific Coast of Mexico. J Invertebr Pathol, 116, 8-12.
Moll, J. R., Ruvinov,S. B., Pastan , I., & Vinson, C. (2001). Designed heterodimerizing leucine zippers with a range of pIs and stabilities up to 10(-15) M. Protein Sci, 10, 649-55.
Msangi, S., Kobayashi, M., Batka, M., Vannuccini, S., Dey, M. M., & Anderson, J. L. (2013). Fish to 2030: Prospects for Fisheries and Aquaculture. World Bank Report(83177-GLB).
Nunan, L. M., Arce, S. M., Staha, R. J., & Lightner, D. V. (2001). Prevalence of infectious hypodermal and hematopoietic necrosis virus (IHHNV) and white spot syndrome virus (WSSV) in Litopenaeus vannamei in the Pacific Ocean off the coast of Panama. Journal of the World Aquaculture Society, 32(3), 330-334.
Ochoa-Solano, J. L., & Olmos-Soto, J. (2006). The functional property of Bacillus for shrimp feeds. Food Microbiol, 23(6), 519-525.
OIE. (2003). Diagnostic Manual for Aquatic Animal Diseases, 4th Editio): OIE Fish Diseases Commission.
Olmedo, P., Hernandez, A. F., Pla, A., Femia, P., Navas-Acien, A., & Gil, F. (2013). Determination of essential elements (copper, manganese, selenium and zinc) in fish and shellfish samples. Risk and nutritional assessment and mercury-selenium balance. Food Chem Toxicol, 62, 299-307.
Osipova, I. G., Sorokulova, I. B., Tereshkina, N. V., & Grigor''eva, L. V. (1997). Safety of bacteria of the genus Bacillus, forming the base of some probiotics. Zhurnal mikrobiologii, epidemiologii, i immunobiologii(6), 68-70.
Owen, J. A., Punt, J., Stranford, S. A., & Jones, P. P. (2013). Kuby immunology: WH Freeman New York.
Pack, P., & Pluckthun, A. (1992). Miniantibodies - Use of Amphipathic Helices to Produce Functional, Flexibly Linked Dimeric Fv Fragments with High Avidity in Escherichia-Coli. Biochemistry, 31, 1579-84.
Park, J. H., Lee, Y. S., Lee, S., & Lee, Y. (1998). An infectious viral disease of penaeid shrimp newly found in Korea. Dis Aquat Organ, 34(1), 71-75.
Proba, K., WoÈrn, A., Honegger, A., & PluÈckthun, A. (1998). Antibody scFv fragments without disulfide bonds, made by molecular evolution. Journal of molecular biology, 275(2), 245-253.
Putnam, F. W., Liu, Y. S., & Low, T. L. (1979). Primary structure of a human IgA1 immunoglobulin. IV. Streptococcal IgA1 protease, digestion, Fab and Fc fragments, and the complete amino acid sequence of the alpha 1 heavy chain. Journal of Biological Chemistry, 254(8), 2865-2874.
Radha, S., & Gunasekaran, P. (2008). Sustained expression of keratinase gene under PxylA and PamyL promoters in the recombinant Bacillus megaterium MS941. Bioresour Technol, 99(13), 5528-5537.
Rajan, P. R., Ramasamy, P., Purushothaman, V., & Brennan, G. P. (2000). White spot baculovirus syndrome in the Indian shrimp Penaeus monodon and P. indicus. Aquaculture, 184(1), 31-44.
Rajendran, K. V., Vijayan, K. K., Santiago, T. C., & Krol, R. M. (1999). Experimental host range and histopathology of white spot syndrome virus (WSSV) infection in shrimp, prawns, crabs and lobsters from India. J Fish Dis, 22(3), 183-191.
Reffetto, M. (2014). Glycemic Index Diet for Dummies: John Wiley & Sons.
Robalino, J., Payne, C., Parnell, P., Shepard, E., Grimes, A. C., Metz, A., Prior, S., Witteveldt, J., Vlak, J. M., Gross, P. S., Warr, G., Browdy, C. L. (2006). Inactivation of white spot syndrome virus (WSSV) by normal rabbit serum: implications for the role of the envelope protein VP28 in WSSV infection of shrimp. Virus Res, 118(1), 55-61.
Sánchez‐Martínez, J. G., Aguirre‐Guzmán, G., & Mejía‐Ruíz, H. (2007). White spot syndrome virus in cultured shrimp: a review. Aquaculture Research, 38(13), 1339-1354.
Sarathi, M., Simon, M. C., Venkatesan, C., & Hameed, A. S. S. (2008). Oral administration of bacterially expressed VP28dsRNA to protect Penaeus monodon from white spot syndrome virus. Mar Biotechnol (NY), 10(3), 242-249.
Shahidi, F., & Brown, J. A. (1998). Carotenoid pigments in seafoods and aquaculture. Crit Rev Food Sci Nutr, 38(1), 1-67.
Sietske, A., & Diderichsen, B. (1991). On the safety of Bacillus subtilis and B. amyloliquefaciens: a review. Appl Microbiol Biotechnol, 36(1), 1-4.
Sila, A., Ghlissi, Z., Kamoun, Z., Makni, M., Nasri, M., Bougatef, A., Sahnoun, Z. (2015). Astaxanthin from shrimp by-products ameliorates nephropathy in diabetic rats. Eur J Nutr, 54(2), 301-307. doi: 10.1007/s00394-014-0711-2
Smith, K. L., & Guentzel, J. L. (2010). Mercury concentrations and omega-3 fatty acids in fish and shrimp: Preferential consumption for maximum health benefits. Mar Pollut Bull, 60(9), 1615-1618.
Song, Y. L., & Hsieh, Y. T. (1994). Immunostimulation of tiger shrimp (Penaeus monodon) hemocytes for generation of microbicidal substances: analysis of reactive oxygen species. Dev Comp Immunol, 18(3), 201-209.
Soto, M. A., & Lotz, J. M. (2001). Epidemiological parameters of white spot syndrome virus infections in Litopenaeus vannamei and L-setiferus. J Invertebr Pathol, 78, 9-15.
Sun, B. Y. (2013). The application of biologics in the prevention of horizontal transmitted White Spot Syndrome Virus (Master''s thesis). Institute of Fisheries Science, College of Life Science, National Taiwan University, 1-91.
Thakur, P. C., Corsin, F., Turnbull, J. F., Shankar, K. M., Hao, N. V., Padiyar, P. A., Madhusudhan, M., Morgan, K. L., Mohan, C. V. (2002). Estimation of prevalence of white spot syndrome virus (WSSV) by polymerase chain reaction in Penaeus monodon postlarvae at time of stocking in shrimp farms of Karnataka, India: a population-based study. Dis Aquat Organ, 49(3), 235-243.
Tu, C., Cheng, S. H., Tsai, J. C., & Tsai, H. J. (2012). Surveillance for Viral Diseases of Farmed Fish and Shrimp - Taiwan, 2012. Exp.Rep AHRI., No.48, 75~82.
Tu, C., Huang, H. T., Chuang, S. H., Hsu, J. P., Kuo, S. T., Li, N. J., Hsu, T. L., Li, M. C., Lin, S. Y. (1999). Taura syndrome in Pacific white shrimp Penaeus vannamei cultured in Taiwan. Dis Aquat Organ, 38(2), 159-161.
van Hulten, M. C., Witteveldt, J., Snippe, M., & Vlak, J. M. (2001). White spot syndrome virus envelope protein VP28 is involved in the systemic infection of shrimp. Virology, 285(2), 228-233.
Vaseeharan, B., & Ramasamy, P. (2003). Control of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon. Lett Appl Microbiol, 36(2), 83-87.
Verma, A. K., Gupta, S., Verma, S., Mishra, A., Nagpure, N. S., Singh, S. P., Pathak, A. K., Sarkar, U. K., Singh, S. P., Singh, M., Seth, P. K. (2013). Interaction between shrimp and white spot syndrome virus through PmRab7-VP28 complex: an insight using simulation and docking studies. J Mol Model, 19(3), 1285-1294.
Wang, C. H., Lo, C. F., Leu, J. H., Chou, C. M., Chou, H. Y., Tung, M. C., Chang, C. F., Su, M. S., Kou, G. H. (1995). Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon. Dis Aquat Organ, 23(3), 239-242.
Wang, Y., Zhang, X., Yuan, L., Xu, T., Rao, Y., Li, J., Dai, H. (2008). Generation of recombinant monoclonal antibodies to study structure-function of envelope protein VP28 of white spot syndrome virus from shrimp. Biochem Biophys Res Commun, 372(4), 902-906.
Ward, E. S. (1992). Antibody engineering using Escherichia coli as host. Advances in pharmacology (San Diego, Calif.), 24, 1-20.
Wonteerasupaya, C., Vickers, J. E., Sriurairatana, S., Nash, G. L., Akarajamorn, A., Boonsaeng, V., Panyim, S., Tassanakajon, A., Withyachumnarnkul, B., Flegel, T. W. (1995). A non-occluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in the black tiger prawn Penaeus monodon. Dis Aquat Organ, 21(1), 69-77.
Wu, J. L., Namikoshi, A., Nishizawa, T., Mushiake, K., Teruya, K., & Muroga, K. (2001). Effects of shrimp density on transmission of penaeid acute viremia in Penaeus japonicus by cannibalism and the waterborne route. Dis Aquat Org, 47, 129-35.
Ye, T., Wu, X., Wu, W., Dai, C., & Yuan, J. (2015). Ferritin protect shrimp Litopenaeus vannamei from WSSV infection by inhibiting virus replication. Fish Shellfish Immunol, 42(1), 138-143.
Yearbook, F. (2012). Fishery and Aquaculture Statistics. Food and Agriculture Organization of the United Nations. Rome, 219.
Yi, G. H., Wang, Z. M., Qi, Y. P., Yao, L. G., Qian, J., & Hu, L. B. (2004). Vp28 of shrimp white spot syndrome virus is involved in the attachment and penetration into shrimp cells. J Biochem Mol Biol, 37(6), 726-734.
Zhang, J. S., Dong, S. L., Dong, Y. W., Tian, X. L., Cao, Y. C., Li, Z. J., & Yan, D. C. (2010). Assessment of the role of brine shrimp Artemia in white spot syndrome virus (WSSV) transmission. Vet Res Commun, 34, 25-32.
Zhang, Y., Ning, J. F., Qu, X. Q., Meng, X. L., & Xu, J. P. (2012). TAT-mediated oral subunit vaccine against white spot syndrome virus in crayfish. J Virol Methods, 181(1), 59-67.
Zou, C., Duan, X., & Wu, J. (2014). Enhanced extracellular production of recombinant Bacillus deramificans pullulanase in Escherichia coli through induction mode optimization and a glycine feeding strategy. Bioresour Technol, 172, 174-179.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
系統版面圖檔 系統版面圖檔